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Full-Text Articles in Veterinary Medicine
Analysis Of Virulence Genes And Pathogenicity Of Thai Mycoplasma Gallisepticum Isolates, Pacharaporn Khumpim, Rapeewan Tampaisarn, Jiroj Sasipreeyajan, Somsak Pakpinyo
Analysis Of Virulence Genes And Pathogenicity Of Thai Mycoplasma Gallisepticum Isolates, Pacharaporn Khumpim, Rapeewan Tampaisarn, Jiroj Sasipreeyajan, Somsak Pakpinyo
The Thai Journal of Veterinary Medicine
Mycoplasma gallisepticum (MG) causes chronic respiratory disease in poultry. MG organisms consist of several virulence genes which are related to infection. The aim of this study was to analyze the virulence genes and determine the pathogenicity of Thai MG isolates. In this study, there were 3 experiments; nineteen MG isolates; and 2 reference strains, F and S6 strains, analyzed to detect 4 virulence genes (LP, gapA, pvpA and mgc2) by PCR assay (experiment 1). The pathogenicity study of sham negative control group, S6 strain, 3 selected isolates (AHRL 31/46, 54/46 and 58/46) and F strain, respectively, were inoculated into 6 …
Infection Of Thai Influenza A Viruses Subtype H5n1 Using Tracheal Culture, Napawan Bunpapong, Supanat Boonyapisitsopa, Kamol Suwannakarn, Rachod Tantilertchareon, Roongroje Thanawongnuwech, Alongkorn Amonsin
Infection Of Thai Influenza A Viruses Subtype H5n1 Using Tracheal Culture, Napawan Bunpapong, Supanat Boonyapisitsopa, Kamol Suwannakarn, Rachod Tantilertchareon, Roongroje Thanawongnuwech, Alongkorn Amonsin
The Thai Journal of Veterinary Medicine
Avian influenza (AI) is caused by influenza A virus subtype H5N1. The virus causes severe disease and death in several avian and mammalian species. This study investigated the ability of swine and chicken tracheal cultures to support highly pathogenic avian influenza subtype H5N1 (HPAI-H5N1) viral replication. Three Thai HPAI-H5N1 viruses isolated from chicken (A/chicken/Thailand/CU-K2/04), duck (A/duck/Thailand/CU-328/07), and tiger (A/tiger/Thailand /CU-T7/04) were used to infect the swine and chicken tracheal cultures. Our results indicated that the chicken tracheal culture could support virus replication in contrast to the swine tracheal culture, suggesting that pig has low susceptibility to Thai HPAI-H5N1 infection. Immunohistochemical …
Genetic Variant Of Elephant Endotheliotropic Herpesvirus Detected From Captive Asian Elephants (Elephas Maximus) In Thailand From 2007 To 2013, Preeda Lertwatcharasarakul, Pornchai Sanyathitiseree, Nikorn Thongtip, Patara Charoenphan3, Benjamas Boonyasart, Nattha Maneewan, Thaweesak Songserm
Genetic Variant Of Elephant Endotheliotropic Herpesvirus Detected From Captive Asian Elephants (Elephas Maximus) In Thailand From 2007 To 2013, Preeda Lertwatcharasarakul, Pornchai Sanyathitiseree, Nikorn Thongtip, Patara Charoenphan3, Benjamas Boonyasart, Nattha Maneewan, Thaweesak Songserm
The Thai Journal of Veterinary Medicine
The study was aimed at characterizing elephant endotheliotropic herpesvirus (EEHV) that was detected in captive Asian elephants in Thailand from 2007 to 2013. Six tissue samples of dead elephants and two EDTA blood samples of surviving elephants in Thailand showed clinical signs or had lesions of the viral infection. Samples were extracted for DNA amplification using a PCR technique with strain specific primers based on terminase and DNA polymerase genes. Six samples gave positive amplicons for EEHV1 specific primers and two samples gave positive amplicons for EEHV3/4 specific primers. Nucleotide sequencing analysis was assured for strain identification. Five out of …