Veterinary Medicine Commons^™

Efflux Of Osmolyte Amino Acids During Isovolumic Regulation In Hippocampal Slices, Rodrigo Franco, Octavio Quesada, Herminia Pasantes-Morales

School of Veterinary and Biomedical Sciences: Faculty Publications

The efflux of potassium (K⁺) and amino acids from hippocampal slices was measured after sudden exposure to 10% (270 mOsm), 25% (225 mOsm) or 50% (150 mOsm) hyposmotic solutions or after gradual decrease (22.5 mOsm/min) in external osmolarity. In slices suddenly exposed to 50% hyposmotic solutions, swelling was followed by partial (74%) cell volume recovery, suggesting regulatory volume decrease (RVD). With gradual hyposmotic changes, no increase in cell water content was observed even when the solution at the end of the experiment was 50% hyposmotic, showing the occurrence of isovolumic regulation (IVR). The gradual decrease in osmolarity elicited …

Equine Laminitis Reviewed, Jocelyn Nelson

Undergraduate Honors Capstone Projects

Laminitis, also known as founder, is one of the most devastating and crippling conditions affecting horses throughout the world today. Traditionally defined as the inflammation of the laminae within the hoof, laminitis actually presents itself as a complicated, multi-system disease, that, through a series of interrelated events, leads to a characteristic lameness. This lameness has serious economic and humane consequences because affected horses are often seen in pain, and frequently must be destroyed due to permanent damages within the hoof.

Ceftriaxone-Resistant Salmonella Infection Acquired By A Child From Cattle, Paul D. Fey, Thomas J. Safranek, Mark E. Rupp, Eileen F. Dunne, Efrain Ribot, Peter C. Iwen, Patricia A. Bradford, Frederick J. Angulo, Steven H. Hinrichs

Zoonotics and Wildlife Disease: Publications

Background
The emergence of resistance to antimicrobial agents within the salmonellae is a worldwide problem that has been associated with the use of antibiotics in livestock. Resistance to ceftriaxone and the fluoroquinolones, which are used to treat invasive salmonella infections, is rare in the United States. We analyzed the molecular characteristics of a ceftriaxone-resistant strain of Salmonella enterica serotype typhimurium isolated from a 12-year-old boy with fever, abdominal pain, and diarrhea.
Methods
We used pulsed-field gel electrophoresis and analysis of plasmids and β-lactamases to compare the ceftriaxone-resistant S. enterica serotype typhimurium from the child with four isolates of this strain …

Genetic Resistance To Experimental Infection With In Red Deer (Cervus Elaphus), Colin G. Mackintosh, Tariq Qureshi, Ken Waldrup, Robert E. Labes, Ken G. Dodds, J. Frank T. Griffin

Michigan Bovine Tuberculosis Bibliography and Database

Tuberculosis (Tb) caused by Mycobacterium bovis is a worldwide threat to livestock and humans. One control strategy is to breed livestock that are more resistant to Mycobacterium bovis. In a 3-year heritability study 6 farmed red deer stags were selected from 39 on the basis of their differing responses to experimental challenge via the tonsillar sac with approximately 500 CFU of M. bovis. Two stags remained uninfected, two were moderately affected, and two developed serious spreading Tb. Seventy offspring, bred from these six stags by artificial insemination using stored semen, were similarly challenged with M. bovis …

Proceedings From The Conference On Bovine Tuberculosis – March 2000

Michigan Bovine Tuberculosis Bibliography and Database

Table of Contents:

The Challenge, USDA by Craig Reed, DVM; John Clifford, DVM

The Comprehensive Strategic Plan by Joe VanTiem, DVM, MS

Surveillance of Non-Cervid (Cattle and Goats) Livestock by Nathan Zauel, DVM

Surveillance of Bovine Tuberculosis in Michigan Captive Cervidae Mike VanderKlok, DVM Surveillance of Domestic Carnivores for Tuberculosis Steven L. Halstead, DVM, MS Human Health Update: Bovine Tuberculosis in Michigan by Barbara Robinson-Dunn, PhD; Mary Grace Stobierski, DVM, MPH

Risks Associated with M. bovis in Michigan Free-ranging White-tailed Deer by Barbara Corso, DVM, MS

Economic Impact of Wildlife-Related Recreation in the 5-County Area: An Update by Dennis Propst, …

The Expression Of P53, C-Erbb-2 And C-Yes In Canine Skin Tumors, Anudep Rungsipipat, Susumu Tateyama, Yuichi Murakami, Kazuyuki Uchida

The Thai Journal of Veterinary Medicine

The exact role of the p53 tumor suppressor gene, c-erbB-2 and c-yes oncogenes in the development of canine skin tumors is still debatable. To elucidate the involvement of these oncogenes in canine skin carcinogenesis, we examined the expression of the protein in 60 skin tumor samples using avidin-biotin complex immunohistochemistry, with polyclonal antibodies directed against each protein, on formalinfixed, paraffin-embedded sections. Positive nuclear staining for p53 protein was observed in 12/60 cases (20%) which included, 6/15(40%) squamous cell carcinomas (SCC) and 4/8(50%) perianal gland tumors (PGT). Out of all the tumors examined, mixed membrane/ cytoplasmic staining of c-erbB-2 protein was …

Artificial Insemination Research Developments In The Pig, Annop Kunavongkrit

The Thai Journal of Veterinary Medicine

Artificial Insemination (AI) in pigs has been well developed especially in the area of instrument design and semen preservation. AI techniques normally practiced as a routine, work well in Thailand swine breeding farms. Current research for the development of AI in pigs at the Department of Obstetrics Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University consists of: fractionated insemination, semen evaluation testing the ability of sperms to penetration oocytes and fertilise and the economic evaluation of AI boars raised in evaporative cooling systems. These researches have been reviewed in this report.

Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn

The Thai Journal of Veterinary Medicine

No abstract provided.

Embryo And Oocyte Collection In Thai Swamp Buffalo (Bubalus Bubalis) After Superovulation, Mongkol Techakumphu, Yant Sukwong, Malee Apimeteetumrong, Siriwat Intaramongkol, Jintana Intaramongkol

The Thai Journal of Veterinary Medicine

Six-day embryos were recovered from 25 swamp buffalo cows treated with pregnant mare serum gonadotropin (2,500 iu PMSG, n=9) and follicle stimulating hormone (280 mg, n=12 and 400 mg FSH, n=4). Two techniques, non surgical collection from live animals and direct flushing after slaughter were compared. The oocytes were collected from ovaries immediately after slaughter by aspiration with or without ovarian chopping. The studies showed that 0.44+0.59 embryos were collected from PMSG group, while 1.38+1.31 embryos were collected in FSH group. Fifty percent to 60% of recovered embryos were transferable. The non surgical technique gave a low recovery rate (14.40%) …

A Preliminary Study On Oocyte Collection Using Transvaginal Ultrasound In Swamp Buffalo Heifers (Bubalas Bubalis), Akachart Promdireg, Mongkol Techakumphu, Nawapen Phutikanit, Ancharlie Na-Chiangmai

The Thai Journal of Veterinary Medicine

The objective of the study was to investigate the possibility of collecting oocytes using transvaginal ultrasound-guidance, in swamp buffalo heifers, after gonadotropin stimulation. Five swamp buffalo heifers were given a single dose of 3,000 IU of Pregnant Mare Serum Gonadotropin (PMSG) intramuscularly in order to stimulate the ovaries. Examination of the ovarian responses were carried out 48h after stimulation, the follicles were subsequently punctured and the oocytes examine under a stereomicroscope. The results of the ovarian response were 6.6 ± 1.1 follicles per animal with an average diameter of 7.1 ± 4.1 mm. The mean number of oocytes collected per …

The Subdural Sinus Of The Freshwater Crocodile (Crocodylus Siamensis), Wimon Pothiwong, Pakorn Prachammuang, Weerapong Koykul

The Thai Journal of Veterinary Medicine

A blood sinus suitable for the collection of blood was studied in four freshwater crocodiles (Crocodylus siamensis). The sinus was located in the neck between the dura mater and the archnoid, which gave it its name "the subdural sinus". The procedure of blood collection in crocodiles is discussed in terms of possible injury to the spinal cord and the chance of getting cerebrospinal fluid mixed into the blood sample.

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.

What Is Your Diagnosis?, Pranee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.

Virus-Induced Neuronal Apoptosis Blocked By The Herpes Simplex Virus Latency-Associated Transcript, Guey-Chuen Perng, Clinton J. Jones, Janice Ciacci-Zanella, Melissa Stone, Gail A. Henderson, Ada Yukht, Susan M. Slanina, Florence M. Hofman, Homayon Ghiasi, Anthony B. Nesburn, Steven L. Wechsler

School of Veterinary and Biomedical Sciences: Faculty Publications

Latent infections with periodic reactivation are a common outcome after acute infection with many viruses. The latency-associated transcript (LAT) gene is required for wild-type reactivation of herpes simplex virus (HSV). However, the underlying mechanisms remain unclear. In rabbit trigeminal ganglia, extensive apoptosis occurred with LAT^- virus but not with LAT⁺ viruses. In addition, a plasmid expressing LAT blocked apoptosis in cultured cells. Thus, LAT promotes neuronal survival after HSV-1 infection by reducing apoptosis.

Agonistic Behaviour And Biogenic Amines In Shore Crabs Carcinus Maenas, Lynne U. Sneddon, Alan C. Taylor, Felicity A. Huntingford, David G. Watson

Sentience Collection

To investigate the role of certain neurohormones in agonistic behaviour, fights were staged between pairs of size-matched male shore crabs Carcinus maenas, and blood samples were taken immediately after the contests had been resolved. Samples were also taken from these crabs at rest (before and after fighting) and after walking on a treadmill. A control group of crabs also had samples taken on each experimental day. Concentrations of tyramine, dopamine, octopamine, serotonin (5-HT) and norepinephrine were determined in each blood sample using a gas chromatography/mass spectrometry (GC-MS) system. Norepinephrine was not detectable in any of the samples, but the standards …

Bulletin 4394 - Exotic Diseases - Sheep And Goats, Agriculture Western Australia

Bulletins 4000 -

An exotic disease is a disease which does not occur in Australia.

If an exotic disease did occur in Australia it would cause production losses, stock deaths and stop exports of primary production. When export markets are lost they can only be re-established once it is proven the disease is eradicated. Some exotic diseases may be transmitted to humans

Prevalence Of Antibody To Hepatitis E Virus Among Rodents In The United States, Michael O. Favorov, Michael Y. Kosoy, Sergei A. Tsarev, James E. Childs, Harold S. Margolis

Zoonotics and Wildlife Disease: Publications

The recent identification of antibody to hepatitis E virus (HEV) in pigs, sheep, and cattle and characterization of an HEV isolated from domestic pigs suggest animal reservoirs for this virus. To investigate whether rodents might be a natural reservoir of HEV, the prevalence of anti-HEV was determined among a variety of species throughout the United States. Serum samples were obtained from 806 rodents of 26 species in 15 genera. Anti-HEV prevalence was assessed by 2 EIAs (mosaic protein- and 55-kDa protein-based), which gave concordant results. The highest prevalence of antibody was found in the genus Rattus (59.7%; 66/278). Overall, rodents …

Incidence Of Rocky Mountain Spotted Fever Among American Indians In Oklahoma, Jennifer H. Mcquiston, Robert C. Holman, Amy V. Groom, Stephen F. Kaufman, James E. Cheek, James E. Childs

Zoonotics and Wildlife Disease: Publications

Althought he state of Oklahoma has traditionally reported very high incidence rates of Rocky Mountain spotted fever (RMSF) cases, the incidence of RMSF among the American Indian population of the state has not been studied. The authors used data from several sources to estimate the incidence of RMSF among American Indians in Oklahoma.

Primary Isolation Of Ehrlichia Chaffeensis From Patients With Febrile Illnesses: Clinical And Molecular Characteristics, Steven M. Standaert, Tina Yu, Margie A. Scott, James E. Childs, Christopher D. Paddock, William L. Nicholson, Joseph Singleton Jr., Martin J. Blaser

Zoonotics and Wildlife Disease: Publications

Ehrlichia chaffeensis was sought among patients with a history of tick exposure and fever, and the accuracy of other diagnostic tests was compared with that of primary isolation. Among the 38 patients enrolled, E. chaffeensis was isolated from the blood of 7 (18%) and from cerebrospinal fluid specimens of 2 of these 7. All 7 patients also were positive by polymerase chain reaction (PCR) of blood, and 6 patients developed diagnostic titers of antibody to E. chaffeensis. The isolates were characterized by molecular analysis of the 16S rRNA gene, the 120-kDa protein gene, and the variable-length PCR target (VLPT) …

Mortality In Serologically Unconfirmed Mediterranean Spotted Fever [With Reply], Pablo Yagupsky, Christopher D. Paddock, James E. Childs, Sherif R. Zaki, Stephen A. Berger

Zoonotics and Wildlife Disease: Publications

I read with interest the article by Paddock et al. [1], who described the detection of occult mortality due to Rocky Mountain spotted fever (RMSF) by demonstrating Rickettsia rickettsii antigens or DNA in blood and tissues. In Mediterranean countries, including Israel, spotted fever is caused by members of the R. conorii complex, which are antigenically related to R. rickettsii. ...

To the Editor -- We appreciate the comments by Yagupsky, which underscore several of the salient features of spotted fever- group rickettsial infections that we emphasized in our report [2]. For patients, diagnosticians, and clinicians faced with the potentially tragic …

Department Of Veterinary And Biomedical Sciences: 2000 Annual Report

School of Veterinary and Biomedical Sciences: Information and History

Foreword, 2000 John A . Schmitz. Professor and Department Head
Veterinary and Biomedical Sciences Personnel
Faculty Profiles
Teaching program
Research program
Extension Program
Nebraska Veterinary and Diagnostic Laboratory Systems
Grants and Contracts Funded or Active in 2000
Patents by VBMS Faculty in 2000
Publications by VBMS Faculty in 2000
Presentations by VBMS Faculty in 2000
Articles Regarding the Department in 2000
Selected Committees, Editorial and Other Appointments of VBMS Faculty
Departmental Budget Summaries
Nebraska Agricultural Statistics 1999

Use Of Gis To Evaluate Livestock-Wildlife Interactions Relative To Tuberculosis Spread On Molokai Island, Hawaii, Stephanie Bany, Jerome Freier

Michigan Bovine Tuberculosis Bibliography and Database

Historically, bovine tuberculosis has occurred and been eradicated several times on the Hawaiian island of Molokai. After the most recent depopulation of cattle, TB was again identified in an adult cow in 1997, thus continuing the investigation into why bovine tuberculosis is reoccurring on the island. The Centers for Epidemiology and Animal Health (CEAH) have implemented spatial analysis using ArcView software that, along with epidemiological investigations, attempts to identify the environmental factors responsible for maintaining this disease in natural foci. To examine the ecological aspects of tuberculosis transmission from wildlife to livestock, and to identify the foci of infection, we …

Quantitative Image Analysis Of Simian Immunodeficiency Virus Replication In Macrophages Coinfected With Mycobacterium Avium Complex, Qingsheng Li, Keith G. Mansfield, Andrew Lackner, Ashley T. Haase

Qingsheng Li Publications

Mycobacterium avium is the most frequent cause of disseminated bacterial infection in patients with human immunodeficiency virus type 1 infection and in rhesus macaques with simian immunodeficiency virus (SIV) infection. This animal model of AIDS was used to test the hypothesis that this frequent association is the result of reciprocal enhancement of replication of both microorganisms. The replication of M. avium and SIV was analyzed in lymphatic tissues obtained from rhesus macaques experimentally inoculated with SIVmac who developed or remained free of overt M. avium infection. In situ hybridization, quantitative image analysis, and staining of M. avium and of macrophages …

Remifentanil In The Horse: Identification And Detection Of Its Major Urinary Metabolite, A. F. Lehner, P. Almeida, J. Jacobs, J Daniel Harkins, W. Karpiesiuk, W. E. Woods, L. Dirikolu, Jeffrey M Bosken, W. G. Carter, J. Boyles, C. Holtz, T. Heller, C. Nattrass, M. Fisher, Thomas Tobin

Maxwell H. Gluck Equine Research Center Faculty Publications

Remifentanil (4-methoxycarbonyl-4-[(1-oxopropyl)phyenylamino]-1- piperidinepropionic acid methyl ester) is a μ-opioid receptor agonist with considerable abuse potential in racing horses. The identification of its major equine urinary metabolite, 4-methoxycarbonyl-4-[(1- oxopropyl)phenylamino]-1-piperidinepropionic acid, an ester hydrolysis product of remifentanil is reported. Administration of remifentanil HCl (5 mg, intravenous) produced clear-cut locomotor responses, establishing the clinical efficacy of this dose. ELISA analysis of postadministration urine samples readily detected fentanyl equivalents in these samples. Mass spectrometric analysis, using solid-phase extraction and trimethylsilyl (TMS) derivatization, showed the urine samples contained parent remifentanil in low concentrations, peaking at 1 h. More significantly, a major peak was identified as …

Effect Of Amitraz And Xylazine On Some Physiological Variables Of Horses, A. Queiroz-Neto, A. B. Carregaro, G. Zamur, J Daniel Harkins, Thomas Tobin, M. I. Mataqueiro, S. C. Gonçalves

Maxwell H. Gluck Equine Research Center Faculty Publications

The effect of the intravenous injection of amitraz (0.1 mg/kg) to horses on cardiac activity, respiratory rate, intestinal motor activity, rectal temperature, sweating and masticating was evaluated and compared to the effect of intravenous administration of xylazine (1mg/kg, iv) on heart rate, respiratory rate and intestinal activity. Amitraz caused a significant decrease in heart activity, respiratory rate and intestinal movements, but these effects were not as pronounced as those caused by xylazine. Amitraz also caused a significant relaxation of the rectal smooth muscle, and an apparent increase in sweating and in the frequency of horses found masticating hay. Rectal temperature …

Comparison Of Postmortem Techniques For The Detection Of Mycobacterium Bovis In White-Tailed Deer (Odocoileus Virginianus), Scott D. Fitzgerald, John B. Kaneene, Kelly L. Butler, Kathy R. Clarke, Jean S. Fierke, Stephen M. Schmitt, Colleen S. Bruning-Fann, Rachel R. Mitchell, Dale E. Berry, Janet B. Payeur

Michigan Bovine Tuberculosis Bibliography and Database

A retrospective study of various diagnostic postmortem techniques used in a 4-year surveillance program for detection of Mycobacterium bovis infection in wild white-tailed deer (Odocoileus virginianus) was conducted. The tests evaluated were routine histopathology, acid-fast staining, detection of acid-fast bacilli in culture, and an M. tuberculosis group-specific genetic probe applied to pure cultures. Each of these techniques were compared with a reference or “gold standard” of mycobacterial culture and identification. Histopathology, the most rapid form of testing for M. bovis infection in white-tailed deer samples, had a sensitivity of 98% and a specificity of 87%, resulting in a …

Isovolumic Regulation In Nervous Tissue: A Novel Mechanism Of Cell Volume Regulation, O. Quesada, R. Franco, K. Hernandez-Fonseca, K. Tuz

School of Veterinary and Biomedical Sciences: Faculty Publications

Cell volume regulation is a property present in most animal cell lineages that allows them to recover their original volume after events of swelling or shrinkage. Such events can be caused by changes in external osmolarity or to osmotic gradients generated during normal cell functioning.4,6 The mechanism of cell volume regulation involves transmembrane fluxes of osmotically active solutes in the necessary direction to counteract the net gain or loss of intracellular water.9 The process through which cells recover their normal volume after swelling is named Regulatory Volume Decrease (RVD). This consists of the efflux of inorganic osmolytes, such as K …

Elaeophorosis In Red Deer From Spain, Mónica Santín-Durán, J. M. Alunda, J. M. San Miguel, Eric P. Hoberg, C. De La Fuente

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Elaeophorosis, caused by Elaeophora elaphi, was observed in red deer (Cervus elaphus) from Toledo Province (Spain) for the first time. Adult specimens of Elaeophora elaphi were found in the hepatic vessels of nine of 151 red deer between October 1994 and September 1995; intensity of infection was two to 18 nematodes per host. Adult nematodes were only found during the period from fall through early spring. No differences were present between sex or age groups. Parasites were not found in a limited sample from fallow deer (Dama dama). Blood samples were negative for the presence of …

The Effects Of Narrowed Area And Additional Feeding On Some Physiological Characteristics Ofhoney Bee (Apis Mellifera L.) Colonies, Ahmet Güler

Turkish Journal of Veterinary & Animal Sciences

The study was conducted at Alata Horticultural Research institute between 1994 and 1996. The aim of the study was to obtain a strong colony by way of supersedure and empty frames, feeding with saccharose syrup and encouraging egg laying of queen bees at the end of summer. According to the results, there were significant differences among treatments in terms of the development of colony population, brood rearing activity and honey yield. Treatments had no significant effect on wax building activity and swarming tendency.

Fattening Performance And Carcass Characteristics Of Akkaraman Crosses (F1 And B1) With German Blackheaded Mutton And Hampshire Down Male Lambs, Ali̇ Akmaz, Emi̇n Teki̇n, Cafer Tepeli̇, Ramazan Kadak

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to investigate the fattening and carcass characteristics of German Blackheaded Mutton (GBM) x Akkaraman (A) F1, GBM x (GBM x A) B1, Hampshire Down (HD) x A F1 and HD x (HD x A) B1 male lambs. Ten GBM x A (F1), 4 GBM x (GBM x A) B1, 10 (HD) x A (F1) and 4 HD x (HD x A) B1 male lambs were used for fattening traits, and six of each breed from F1 genotypes, four of each breed from B1 genotypes for carcass traits. The lambs were fed, from the beginning at …