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Full-Text Articles in Veterinary Medicine

The Human Ehrlichioses In The United States, Jennifer H. Mcquiston, Christopher D. Paddock, Robert C. Holman, James E. Childs Jan 1999

The Human Ehrlichioses In The United States, Jennifer H. Mcquiston, Christopher D. Paddock, Robert C. Holman, James E. Childs

Zoonotics and Wildlife Disease: Publications

The emerging tick-borne zoonoses human monocytic ehrlichiosis (HME) and human granulocytic ehrlichiosis (HGE) are underreported in the United States. From 1986 through 1997, 1,223 cases (742 HME, 449 HGE, and 32 not ascribed to a specific ehrlichial agent) were reported by state health departments. HME was most commonly reported from southeastern and southcentral states, while HGE was most often reported from northeastern and upper midwestern states. The annual number of reported cases increased sharply, from 69 in 1994 to 364 in 1997, coincident with an increase in the number of states making these conditions notifiable. From 1986 through 1997, 827 …


Long-Term Studies Of Hantavirus Reservoir Populations In The Southwestern United States: A Synthesis, James N. Mills, Thomas G. Ksiazek, C.J. Peters, James E. Childs Jan 1999

Long-Term Studies Of Hantavirus Reservoir Populations In The Southwestern United States: A Synthesis, James N. Mills, Thomas G. Ksiazek, C.J. Peters, James E. Childs

Zoonotics and Wildlife Disease: Publications

A series of intensive, longitudinal, mark-recapture studies of hantavirus infection dynamics in reservoir populations in the southwestern United States indicates consistent patterns as well as important differences among sites and host-virus associations. All studies found a higher prevalence of infection in older (particularly male) mice; one study associated wounds with seropositivity. These findings are consistent with horizontal transmission and transmission through fighting between adult male rodents. Despite very low rodent densities at some sites, low-level hantavirus infection continued, perhaps because of persistent infection in a few long-lived rodents or periodic reintroduction of virus from neighboring populations. Prevalence of hantavirus antibody …


Diagnosis Of Human Ehrlichiosis By Pcr Assay Of Acute-Phase Serum, James A. Comer, William L. Nicholson, John W. Sumner, James G. Olson, James E. Childs Jan 1999

Diagnosis Of Human Ehrlichiosis By Pcr Assay Of Acute-Phase Serum, James A. Comer, William L. Nicholson, John W. Sumner, James G. Olson, James E. Childs

Zoonotics and Wildlife Disease: Publications

A PCR assay of 43 acute-phase serum samples was evaluated as a method for early detection of human granulocytic ehrlichiosis (HGE) and determination of etiology when serologic testing is inconclusive. Sequenceconfirmed products of the HGE agent were amplified from three individuals residing or having exposure history in Minnesota or Wisconsin, and similarly confirmed products from Ehrlichia chaffeensis were amplified from three individuals from Florida or Maryland. Etiology, as determined by PCR and serology, was the same whenever there was a fourfold difference between the maximum titers of antibodies to both antigens, indicating that presumptive determination of etiology may be based …


Dusky-Footed Wood Rats (Neotoma Fuscipes) As Reservoirs Of Granulocytic Ehrlichiae (Rickettsiales: Ehrlichieae) In Northern California, William L. Nicholson, Martin B. Castro, Vicki L. Kramer, John W. Sumner, James E. Childs Jan 1999

Dusky-Footed Wood Rats (Neotoma Fuscipes) As Reservoirs Of Granulocytic Ehrlichiae (Rickettsiales: Ehrlichieae) In Northern California, William L. Nicholson, Martin B. Castro, Vicki L. Kramer, John W. Sumner, James E. Childs

Zoonotics and Wildlife Disease: Publications

Dusky-footed wood rats (Neotoma fuscipes) and Peromyscus sp. mice (P. maniculatus and P. truei) were collected from one site in Placer County, one site in Santa Cruz County, and two sites in Sonoma County in northern California. Serum or plasma samples from 260 rodents were tested for antibodies to the agent of human granulocytic ehrlichiosis. Of these, samples from 25 wood rats (34% of those tested) and 10 (8%) Peromyscus sp. mice were found to be seropositive, but only those from one site. PCR assays targeting the groESL heat shock operon were conducted on all seropositive …


Outcome Of Diagnostic Tests Using Samples From Patients With Culture-Proven Human Monocytic Ehrlichiosis: Implications For Surveillance, James E. Childs, John W. Sumner, William L. Nicholson, Robert F. Massung, Steven M. Standaert, Christopher D. Paddock Jan 1999

Outcome Of Diagnostic Tests Using Samples From Patients With Culture-Proven Human Monocytic Ehrlichiosis: Implications For Surveillance, James E. Childs, John W. Sumner, William L. Nicholson, Robert F. Massung, Steven M. Standaert, Christopher D. Paddock

Zoonotics and Wildlife Disease: Publications

We describe the concordance among results from various laboratory tests using samples derived from nine culture-proven cases of human monocytic ehrlichiosis (HME) caused by Ehrlichia chaffeensis. A class-specific indirect immunofluorescence assay for immunoglobulin M (IgM) and IgG, using E. chaffeensis antigen, identified 44 and 33% of the isolation-confirmed HME patients on the basis of samples obtained at initial clinical presentation, respectively; detection of morulae in blood smears was similarly insensitive (22% positive). PCR amplifications of ehrlichial DNA targeting the 16S rRNA gene, the variable-length PCR target gene, and the groESL operon were positive for whole blood specimens obtained …


Hidden Mortality Attributable To Rocky Mountain Spotted Fever: Immunohistochemical Detection Of Fatal, Serologically Unconfirmed Disease, James E. Childs, Patricia W. Greer, Tara L. Ferebee, Joseph Singleton Jr., Don B. Mckechnie, Tracee A. Treadwell, John W. Krebs, Matthew J. Clarke, Robert C. Holman, James G. Olson, Christopher D. Paddock, Sherif R. Zaki Jan 1999

Hidden Mortality Attributable To Rocky Mountain Spotted Fever: Immunohistochemical Detection Of Fatal, Serologically Unconfirmed Disease, James E. Childs, Patricia W. Greer, Tara L. Ferebee, Joseph Singleton Jr., Don B. Mckechnie, Tracee A. Treadwell, John W. Krebs, Matthew J. Clarke, Robert C. Holman, James G. Olson, Christopher D. Paddock, Sherif R. Zaki

Zoonotics and Wildlife Disease: Publications

Rocky Mountain spotted fever (RMSF) is the most severe tickborne infection in the United States and is a nationally notifiable disease. Since 1981, the annual case-fatality ratio for RMSF has been determined from laboratory-confirmed cases reported to the Centers for Disease Control and Prevention (CDC). Herein, a description is given of patients with fatal, serologically unconfirmed RMSF for whom a diagnosis of RMSF was established by immunohistochemical (IHC) staining of tissues obtained at autopsy. During 1996-1997, acute-phase serum and tissue samples from patients with fatal disease compatible with RMSF were tested at the CDC. As determined by indirect immunofluorescence assay, …


Search For The Ebola Virus Reservoir In Kikwit, Democratic Republic Of The Congo: Reflections On A Vertebrate Collection, Herwig Leirs, James N. Mills, John W. Krebs, James E. Childs, Dudu Akaibe, Neal Woollen, George Ludwig, Clarence J. Peters, Thomas G. Ksiazek Jan 1999

Search For The Ebola Virus Reservoir In Kikwit, Democratic Republic Of The Congo: Reflections On A Vertebrate Collection, Herwig Leirs, James N. Mills, John W. Krebs, James E. Childs, Dudu Akaibe, Neal Woollen, George Ludwig, Clarence J. Peters, Thomas G. Ksiazek

Zoonotics and Wildlife Disease: Publications

A 3-month ecologic investigation was done to identify the reservoir of Ebola virus following the 1995 outbreak in Kikwit, Democratic Republic of the Congo. Efforts focused on the fields where the putative primary case had worked but included other habitats near Kikwit. Samples were collected from 3066 vertebrates and tested for the presence of antibodies to Ebola (subtype Zaire) virus: All tests were negative, and attempts to isolate Ebola virus were unsuccessful. The investigation was hampered by a lack of information beyond the daily activities of the primary case, a lack of information on Ebola virus ecology, which precluded the …


Population Dynamics Of A Naturally Occurring Heterogeneous Mixture Of Borrelia Burgdorferi Clones, Erick K. Hofmeister, Gregory E. Glass, James E. Childs, David H. Persing Jan 1999

Population Dynamics Of A Naturally Occurring Heterogeneous Mixture Of Borrelia Burgdorferi Clones, Erick K. Hofmeister, Gregory E. Glass, James E. Childs, David H. Persing

Zoonotics and Wildlife Disease: Publications

Two unique isolates of Borrelia burgdorferi, differing in plasmid content and outer surface protein C expression, were cultured on sequential captures of a single free-living Peromyscus leucopus mouse and were examined for differences in transmissibility. Both isolates were transmissible from inoculated C.B-17 mice to larval Ixodes scapularis ticks and, subsequently, from infected nymphal ticks to C3H/HeJ mice. Plasmid and protein analyses suggested that the original isolates were a mixed population of B. burgdorferi, and cloning by limiting dilution resulted in the identification of two clonal groups. In addition to being heterogeneous in plasmid and genomic macrorestriction analyses, the …


Isolation Of A New Subspecies, Bartonella Vinsonii Subsp. Arupensis, From A Cattle Rancher: Identity With Isolates Found In Conjunction With Borrelia Burgdorferi And Babesia Microti Among Naturally Infected Mice, David F. Welch, Karen C. Carroll, Erick K. Hofmeister, David H. Persing, Denise A. Robison, Arnold G. Steigerwalt, Don J. Brenner Jan 1999

Isolation Of A New Subspecies, Bartonella Vinsonii Subsp. Arupensis, From A Cattle Rancher: Identity With Isolates Found In Conjunction With Borrelia Burgdorferi And Babesia Microti Among Naturally Infected Mice, David F. Welch, Karen C. Carroll, Erick K. Hofmeister, David H. Persing, Denise A. Robison, Arnold G. Steigerwalt, Don J. Brenner

Zoonotics and Wildlife Disease: Publications

Bacteremia with fever due to a novel subspecies of Bartonella vinsonii was found in a cattle rancher. The subspecies shared major characteristics of the genus Bartonella in terms of most biochemical features and cellular fatty acid profile, but it was distinguishable from other subspecies of B. vinsonii by good growth on heart infusion agar supplemented with X factor and by its pattern of enzymatic hydrolysis of peptide substrates. DNA relatedness studies verified that the isolate belonged to the genus Bartonella and that it was genotypically related to B. vinsonii. The highest level of relatedness was observed with recently characterized …


Oral Transmission And Early Lymphoid Tropism Of Chronic Wasting Disease PrpRes In Mule Deer Fawns (Odocoileus Hemionus), Christina J. Sigurdson, Elizabeth S. Williams, Michael W. Miller, Terry R. Spraker, Katherine I. O'Rourke, Edward A. Hoover Jan 1999

Oral Transmission And Early Lymphoid Tropism Of Chronic Wasting Disease PrpRes In Mule Deer Fawns (Odocoileus Hemionus), Christina J. Sigurdson, Elizabeth S. Williams, Michael W. Miller, Terry R. Spraker, Katherine I. O'Rourke, Edward A. Hoover

Zoonotics and Wildlife Disease: Publications

Mule deer fawns (Odocoileus hemionus) were inoculated orally with a brain homogenate prepared from mule deer with naturally occurring chronic wasting disease (CWD), a prion-induced transmissible spongiform encephalopathy. Fawns were necropsied and examined for PrPres, the abnormal prion protein isoform, at 10, 42, 53, 77, 78 and 80 days post-inoculation (p.i.) using an immunohistochemistry assay modified to enhance sensitivity. PrPres was detected in alimentarytract- associated lymphoid tissues (one or more of the following: retropharyngeal lymph node, tonsil, Peyer’s patch and ileocaecal lymph node) as early as 42 days p.i. and in all fawns examined thereafter …


Prp Genotypes Of Captive And Free-Ranging Rocky Mountain Elk (Cervus Elaphus Nelsoni) With Chronic Wasting Disease, Katherine I. O'Rourke, T. E. Besser, M. W. Miller, T. F. Cline, T. R. Spraker, A. L. Jenny, M. A. Wild, G. L. Zebarth Jan 1999

Prp Genotypes Of Captive And Free-Ranging Rocky Mountain Elk (Cervus Elaphus Nelsoni) With Chronic Wasting Disease, Katherine I. O'Rourke, T. E. Besser, M. W. Miller, T. F. Cline, T. R. Spraker, A. L. Jenny, M. A. Wild, G. L. Zebarth

Zoonotics and Wildlife Disease: Publications

The PrP gene encodes the putative causative agent of the transmissible spongiform encephalopathies (TSEs), a heterogeneous group of fatal, neurodegenerative disorders including human Creutzfeldt–Jakob disease, bovine spongiform encephalopathy, ovine scrapie and chronic wasting disease (CWD) of North American deer and elk. Polymorphisms in the PrP gene are associated with variations in relative susceptibility, pathological lesion patterns, incubation times and clinical course of TSEs of humans, mice and sheep. Sequence analysis of the PrP gene from Rocky Mountain elk showed only one amino acid change (Met to Leu at cervid codon 132). Homozygosity for Met at the corresponding polymorphic site (Met …


Family Cluster Of Rocky Mountain Spotted Fever, James E. Childs, Timothy F. Jones, Allen S. Craig, Christopher D. Paddock, Don B. Mckechnie, Sherif R. Zaki, William Schaffner Jan 1999

Family Cluster Of Rocky Mountain Spotted Fever, James E. Childs, Timothy F. Jones, Allen S. Craig, Christopher D. Paddock, Don B. Mckechnie, Sherif R. Zaki, William Schaffner

Zoonotics and Wildlife Disease: Publications

Soon after a patient from Tennessee died of Rocky Mountain spotted fever (RMSF), several family members developed symptoms suggestive of the disease and were treated presumptively for RMSF. Fifty-four persons visiting the index patient's home were interviewed; serum samples were collected from 35. Three additional cases of RMSF were confirmed, all of which occurred in firstdegree relatives. Time spent at the family home and going into the surrounding woods were significantly associated with developing antibodies to Rickettsia rickettsii. Ticks were collected and examined for rickettsiae by polymerase chain reaction analysis. Because hyperendemic foci and family clusters of RMSF can …


Molecular Cloning And Characterization Of The Ehrlichia Chaffeensis Variable-Length Pcr Target: An Antigen-Expressing Gene That Exhibits Interstrain Variation, John W. Sumner, James E. Childs, Christopher D. Paddock Jan 1999

Molecular Cloning And Characterization Of The Ehrlichia Chaffeensis Variable-Length Pcr Target: An Antigen-Expressing Gene That Exhibits Interstrain Variation, John W. Sumner, James E. Childs, Christopher D. Paddock

Zoonotics and Wildlife Disease: Publications

A clone expressing an immunoreactive protein with an apparent molecular mass of 44 kDa was selected from an Ehrlichia chaffeensis Arkansas genomic library by probing with anti-E. chaffeensis hyperimmune mouse ascitic fluid. Nucleotide sequencing revealed an open reading frame (ORF) capable of encoding a 198-aminoacid polypeptide. The ORF contained four imperfect, direct, tandem 90-bp repeats. The nucleotide and deduced amino acid sequences did not show close homologies to entries in the molecular databases. PCR with primers whose sequences matched the sequences flanking the ORF was performed with DNA samples extracted from cell cultures infected with nine different isolates of E. …


Serologic Testing For Human Granulocytic Ehrlichiosis At A National Referral Center, James A. Comer, William L. Nicholson, James G. Olson, James E. Childs Jan 1999

Serologic Testing For Human Granulocytic Ehrlichiosis At A National Referral Center, James A. Comer, William L. Nicholson, James G. Olson, James E. Childs

Zoonotics and Wildlife Disease: Publications

An indirect immunofluorescence assay (IFA) was used to identify patients with antibodies reactive to the human granulocytic ehrlichiosis (HGE) agent. Serum samples collected from clinically ill individuals were submitted to the Centers for Disease Control and Prevention by physicians via state health departments from throughout the United States and tested against a panel of ehrlichial and rickettsial pathogens. Antibodies reactive to the HGE agent were detected in 142 (8.9%) of 1,602 individuals tested. There were 19 confirmed and 59 probable (n = 78) cases of HGE as defined by seroconversion or a fourfold or higher titer to the HGE agent …


Monitoring Of Transmission Of Tuberculosis Between Wild Boars And Cattle: Genotypical Analysis Of Strains By Molecular Epidemiology Techniques, Andrea Serraino, Giulia Marchetti, Valeria Sanguinetti, Maria Cristina Rossi, Renato Giulio Zanoni, Lidia Catozzi, Alessandra Bandera, Walter Dini, Walter Mignone, Fabio Franzetti, Andrea Gori Jan 1999

Monitoring Of Transmission Of Tuberculosis Between Wild Boars And Cattle: Genotypical Analysis Of Strains By Molecular Epidemiology Techniques, Andrea Serraino, Giulia Marchetti, Valeria Sanguinetti, Maria Cristina Rossi, Renato Giulio Zanoni, Lidia Catozzi, Alessandra Bandera, Walter Dini, Walter Mignone, Fabio Franzetti, Andrea Gori

Michigan Bovine Tuberculosis Bibliography and Database

An epidemiological survey for the monitoring of bovine tuberculosis transmission was carried out in western Liguria, a region in northern Italy. Fifteen Mycobacterium bovis strains were isolated from 63 wild boar samples (62 from mandibular lymph nodes and 1 from a liver specimen). Sixteen mediastinal lymph nodes of 16 head of cattle were collected, and 15 Mycobacterium bovis strains were subsequently cultured. All M. bovisstrains isolated from cattle and wild boars were genotyped by spoligotyping and by restriction fragment length polymorphism (RFLP) analysis with the IS6110 and IS1081 probes. All M. bovis strains showed the typical spoligotype characterized by …


Veterinary And Biomedical Sciences: 1999 Annual Report Jan 1999

Veterinary And Biomedical Sciences: 1999 Annual Report

School of Veterinary and Biomedical Sciences: Information and History

VBMS Personnel
VBMS Postdoctoral Research Associates
Awards and Recognition
Committee Assignments
Teaching Program
Graduate Program
Great Plains Veterinary Educational Center (GPVEC)
Research Program
Extension Program
Nebraska Veterinary Diagnostic Laboratory Systems (NVDLS)
Grants
Publications
Presentations
Selected Committees, Editorial and Other Appointments
Departmental Budget Summaries
Nebraska Agricultural Statistics
Appendix


Development Of A Model Of Natural Infection With Mycobacterium Bovis In White-Tailed Deer, Mitchell V. Palmer, Diana L. Whipple, Steven C. Olsen Jan 1999

Development Of A Model Of Natural Infection With Mycobacterium Bovis In White-Tailed Deer, Mitchell V. Palmer, Diana L. Whipple, Steven C. Olsen

Michigan Bovine Tuberculosis Bibliography and Database

The objective of this study was to develop a suitable experimental model of natural Mycobacterium bovis infection in white-tailed deer (Odocoileus virginianus), describe the distribution and character of tuberculous lesions, and to examine possible routes of disease transmission. In October 1997, 10 mature female white-tailed deer were inoculated by intratonsilar instillation of 2 3 103 (low dose) or 2 3 105 (high dose) colony forming units (CFU) of M. bovis. In January 1998, deer were euthanatized, examined, and tissues were collected 84 to 87 days post inoculation. Possible routes of disease transmission were evaluated by culture of nasal, …


Diagnosis Of Ovulation Rate And Embriyonal, Foetal Development By Transrectalultrasonography In The White Goats, Di̇lek Arsoy Başaran Jan 1999

Diagnosis Of Ovulation Rate And Embriyonal, Foetal Development By Transrectalultrasonography In The White Goats, Di̇lek Arsoy Başaran

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to check the ovulation rate, early pregnancy diagnosis, and the number of embryos and foetuses, and embryonic and foetal development using either FGA with a low dose PMSG(250 I.U.) or FGA without of PMSG in 2.5- 5.5-year-old white goats (Saanen X Kilis).Transrectal ultrasonography with a 5-7.5 MHz linear probe was used 7, 26, 33, 40, 50 and 57 days after mating. The ovulation rate was determined by the counting of corpora lutea in both of the ovaries 7 days after mating. The ovulation rate at day 7 in the PMSG group was (SE±)10.833±0.79, and …


Effects Of Dietary Supplementation With Organic Acids And Zinc Bacitracinon Ileal Microflora, Ph And Performance In Broilers, Müjdat Alp, Neşe Kocabağli, Recep Kahraman, Kami̇l Bostan Jan 1999

Effects Of Dietary Supplementation With Organic Acids And Zinc Bacitracinon Ileal Microflora, Ph And Performance In Broilers, Müjdat Alp, Neşe Kocabağli, Recep Kahraman, Kami̇l Bostan

Turkish Journal of Veterinary & Animal Sciences

An experiment was conducted to determine the effects of an organic acid combination (Acid Lac Dry: lactic, fumaric, propionic, citric and formic acid) and/or zinc bacitracin on the body weight gain, feed efficiency, microflora and pH in the ileum content of broiler-type chicks. Ross PM3 broiler chicks were placed in an experimental design that consisted of four dietary treatments with 48 broilers in each group. One-day-old chicks were fed either a control diet or a similar diet supplemented with 3 g/kg Acid Lac Dry, 0.1 g/kg zinc bacitracin or both additives in combination during a 42-d feeding trial. Acid Lac …


Parafilariosis In Cattle In Malatya And Environs, Cem Ecmel Şaki̇, Murat Sevgi̇li̇, Edi̇p Özer Jan 1999

Parafilariosis In Cattle In Malatya And Environs, Cem Ecmel Şaki̇, Murat Sevgi̇li̇, Edi̇p Özer

Turkish Journal of Veterinary & Animal Sciences

This study was conducted in cattle on Malatya and environs. A total of 432 cattle were clinically examined for cattle parafilariosis. Cattle parafilariosis was clinically diagnosed in 22 cattle in August 1995, six cattle in September 1995, two cattle in July 1996, one cattle in August 1996, and one cattle in August 1997. Thirty-two out of 432 cattle (7.4%) were diagnosed as having the disease. Upon the microscopic examination of blood samples from wounds, 18 microfilariae were found whereas no eggs were found in 10 cattle. The disease was more widespread in 1-2 year-old cattle (46.8%) and females (68.7%) than …


A Study On Rearing Of Rainbow Trout (Oncorhynchus Mykiss) In The Net Cages In Almus Dam Lake, Mehmet Karataş, A. İhsan Toğlaci Jan 1999

A Study On Rearing Of Rainbow Trout (Oncorhynchus Mykiss) In The Net Cages In Almus Dam Lake, Mehmet Karataş, A. İhsan Toğlaci

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the growth parameters of Rainbow trout (Oncorhynhcus mykiss) in net cages in Almus Dam Lake. The experiment continued for a period of 105 days between May 1 and August 15 1996. During the experiment 6480 rainbow trout with an average weight of 50 g were used. Experimental groups of 50 g mean initial body weight reached 279 g mean final weight by the end of the experiment.


Arsenic Residues In Grey Mullets Caught In Lake Van, Ali̇ Bi̇lgi̇li̇, Ender Yarsan, İdri̇s Türel Jan 1999

Arsenic Residues In Grey Mullets Caught In Lake Van, Ali̇ Bi̇lgi̇li̇, Ender Yarsan, İdri̇s Türel

Turkish Journal of Veterinary & Animal Sciences

In this study, pollution caused by arsenic residues in grey mullets (Chalcarburnus tarichii, Pallas 1811) living in Van Lake were investigated. A Total 120 samples, from the four seasons from 01.01.1994 to 01.01.1995 were collected. The organic parts of the muscles were washed with a mixture of magnesium oxide and magnesium nitrate according to the dry ash method and arsenic residues were measured by silver diethyl dithyocarbamate in a spectrophotometer. By analyzing the 120 fish samples, arsenic residues were calculated to be 0.009 - 0.347 ppm (average 0.051±0.046). In light of these results as the arsenic residues in grey mullets …


Detection Of Listeria Species In Minced Beef, Ufuk Tansel Şi̇reli̇, İrfan Erol Jan 1999

Detection Of Listeria Species In Minced Beef, Ufuk Tansel Şi̇reli̇, İrfan Erol

Turkish Journal of Veterinary & Animal Sciences

In recent years there has been an increase in the number of outbreaks of human listeriosis and contaminated foods have been considered to be a potential source of infection. In this study, a total of 100 minced beef samples were obtained from different butchers and supermarkets in Ankara and examined for the presence and degree of contamination with Listeria species. The minced beef samples were collected in aseptic conditions and transported to the laboratory in cold storage. The method suggested by USDA - FSIS was used for the isolation and identification of Listeria spp. In addition, the degree of contamination …


Determination Of Cyanide Levels In Sorghum Halepense At Different Vegetation Stages In The Aydın Area, Mustafa Sari, Ferda Akar, Fatma Karataş Jan 1999

Determination Of Cyanide Levels In Sorghum Halepense At Different Vegetation Stages In The Aydın Area, Mustafa Sari, Ferda Akar, Fatma Karataş

Turkish Journal of Veterinary & Animal Sciences

In this study, HCN levels in 150 Sorghum halepense samples obtained from the province of Aydın during 3 different vegetation periods were determined. The analysis was carried out using spectrophotometry. According to the results of the analysis, average HCN levels during the tiller, flowering, and postflowering stages in Sorghum halepense leaves, were 460.8, 144.8, and 44.22 ppm respectively. It was concluded that HCN levels in Sorghum halepense growing in the province of Aydın would be toxic for herbivores during the tillers and flowering stages.


Characterization Of Monoclonal Antibodies Againstfoot And Mouth Disease Virus And Determination Of Antigenicvariations In Field Strains, S. İsmet Gürhan, Burhan Gürhan, Gülhan Aynagöz, Ni̇lay Ünal, Gülnur Ünver Jan 1999

Characterization Of Monoclonal Antibodies Againstfoot And Mouth Disease Virus And Determination Of Antigenicvariations In Field Strains, S. İsmet Gürhan, Burhan Gürhan, Gülhan Aynagöz, Ni̇lay Ünal, Gülnur Ünver

Turkish Journal of Veterinary & Animal Sciences

The aim of this project was characterization of monoclonal antibodies (MAbs) against O 1 Man. 69, a vaccine strain of the Ankara Foot and Mouth Disease (FMD) Institute and detection of the antigenic variation in field virus strains. For that purpose,17 MAbs were used. Activities of MAbs against 146S, enzyme treated 146S (146S-T) and 12S antigenic particles of O 1 Man. 69 virus strain were estimated by indirect ELISA. Reaction to viral capsid proteins was detected by Western immunoblotting and detection of the neutralization specificity was done by both in vitro and in vivo neutralization tests and mouse protection assay. …


Factors Affecting Milk Composition And Changes In Milkcomposition With Lactation Stage In Anatolian Buffaloes, Özel Şekerden, Hüseyi̇n Erdem, Bülent Kankurdan, Bülent Özlü Jan 1999

Factors Affecting Milk Composition And Changes In Milkcomposition With Lactation Stage In Anatolian Buffaloes, Özel Şekerden, Hüseyi̇n Erdem, Bülent Kankurdan, Bülent Özlü

Turkish Journal of Veterinary & Animal Sciences

The research was performed to investigate factors affecting milk composition and chaning of milk components with lactation stage in Anatolian buffaloes. The material of the research was formed by 109 milk samples belonging to 28 cows rasied at 12 units of 3 seperate village of Çarsamba District of Samsun Province. Milk samples were taken at AM milkings in one month intervals. Lactation stages' effect was found significant level statistically (P


Effect Of Alcohol Treatment On In Situ Degradabilities Of Some Protein Sources, B. Zehra Sariçi̇çek Jan 1999

Effect Of Alcohol Treatment On In Situ Degradabilities Of Some Protein Sources, B. Zehra Sariçi̇çek

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to determine the effect of alcohol treatment on in situ degradabilities of some protein sources. While the dry matter degradabilities (DMP) of hazelnut kernel oil meal (HKOM), soybean meal (SBM), sunflower meal (SM) and full-fat soy (FFS) for 24 hour incubation period were found to be 86.06, 86.01, 70.66 and 70.21 %, the corresponding values for alcohol treatment were 74.63, 79.41, 61.26 and 63.41%, respectively. For the same incubation period rumen organic matter degradabilities (OMD) of untreated feeds and alcohol treated feeds were found to be 85.20, 90.32, 70.96, 71.29 % and 75.61, 72.24, 62.54 and …


The Effects Of Different Fat Sources On Performance And Fat Deposition Of Broilers, Fi̇gen Kirkpinar, A. Mehmet Taluğ, Ramazan Erkek, Fevzi̇ Sevgi̇can Jan 1999

The Effects Of Different Fat Sources On Performance And Fat Deposition Of Broilers, Fi̇gen Kirkpinar, A. Mehmet Taluğ, Ramazan Erkek, Fevzi̇ Sevgi̇can

Turkish Journal of Veterinary & Animal Sciences

Sunflower oil, cotton seed oil, soybean oil, sunflower oil soapstock, and tallow singly or in combination (1:1) with tallow were added to starter and finisher diets fed to 414 broiler chickens. Nine different isonitrogenic and isocaloric diets were prepared. Body weight differences were significant (P


Letter To The Editor, Nursal Meti̇n Jan 1999

Letter To The Editor, Nursal Meti̇n

Turkish Journal of Veterinary & Animal Sciences

No abstract provided.


Observations On Animal And Human Health During The Outbreak Of Mycobacterium Bovis In Game Farm Wapiti In Alberta, P. Nick Nation, E. Anne Fanning, H. Bim Hopf, Terry L. Church Jan 1999

Observations On Animal And Human Health During The Outbreak Of Mycobacterium Bovis In Game Farm Wapiti In Alberta, P. Nick Nation, E. Anne Fanning, H. Bim Hopf, Terry L. Church

Michigan Bovine Tuberculosis Bibliography and Database

This report describes and discusses the history, clinical, pathologic, epidemiologic, and human health aspects of an outbreak of Mycobacterium bovis infection in domestic wapiti in Alberta between 1990 and 1993, shortly after legislative changes allowing game farming. The extent and seriousness of the outbreak of M. bovis in wapiti in Alberta was not fully known at its onset. The clinical findings in the first recognized infected wapiti are presented and the postmortem records for the herd in which the animal resided are summarized. Epidemiologic findings from the subsequent field investigation are reviewed, the results of recognition and investigation of human …