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Full-Text Articles in Veterinary Medicine
Phenotypic And Genotypic Characterization Of Antimicrobial Resistance In Commonly Isolated Salmonella Serovars From Chickens, Seyyi̇de Sariçam İnce, Mehmet Akan
Phenotypic And Genotypic Characterization Of Antimicrobial Resistance In Commonly Isolated Salmonella Serovars From Chickens, Seyyi̇de Sariçam İnce, Mehmet Akan
Turkish Journal of Veterinary & Animal Sciences
Salmonellosis caused by Salmonella agents is the second most common zoonotic infection in humans. In recent years, Salmonella's increasing antimicrobial resistance (AMR) has been a concern. The major transmission route of Salmonella is consumption of contaminated poultry products. Therefore, monitoring of antimicrobial resistance in chicken-originated Salmonella is critically important. This study investigated AMR in four commonly isolated Salmonella serovars from chickens, namely Salmonella Enteritidis (S. Enteritidis), Salmonella Infantis (S. Infantis), Salmonella Kentucky (S. Kentucky), and Salmonella Typhimurium (S. Typhimurium). A total of 133 isolates were examined by phenotypic and genotypic AMR characterization. Resistance to 14 different antimicrobials and eight …
A Surveillance For Avian Coronavirus Infectious Bronchitis Virus, Infectious Laryngotracheitis Virus, Avian Metapneumovirus, And Avian Reovirus In Poultry Flocks With Respiratory Signs In Türkiye, Özge Ardiçli, Serpi̇l Kahya Demi̇rbi̇lek, Fethi̇ye Çöven, Kami̇l Tayfun Carli
A Surveillance For Avian Coronavirus Infectious Bronchitis Virus, Infectious Laryngotracheitis Virus, Avian Metapneumovirus, And Avian Reovirus In Poultry Flocks With Respiratory Signs In Türkiye, Özge Ardiçli, Serpi̇l Kahya Demi̇rbi̇lek, Fethi̇ye Çöven, Kami̇l Tayfun Carli
Turkish Journal of Veterinary & Animal Sciences
In this study, avian coronavirus infectious bronchitis virus (IBV), infectious laryngotracheitis virus (ILTV), avian metapneumovirus (AMPV), and avian reovirus (ARV) were evaluated in broiler and layer flocks. For this purpose, tracheal swabs from 48 broiler and 45 layer flocks with respiratory signs were inoculated SPF embryonated chicken eggs for virus isolation. The viruses were identified by real-time PCR. Results showed that the most common virus in both broiler and layer farms was IBV with incidence rates of 58.33% and 46.67%, respectively. ILTV, AMPV, and ARV incidences in the samples were found to be 22.22%, 13.33%, and 4.44% in layer flocks …
Ginkgo Biloba Extract Egb761 Improved Anti-Heat Stress Responses In Chickens In Vivo Via Regulation Of Heat-Shock Protein Expression And Distribution, Xiaohui Zhang, Miao Zhang, Endong Bao
Ginkgo Biloba Extract Egb761 Improved Anti-Heat Stress Responses In Chickens In Vivo Via Regulation Of Heat-Shock Protein Expression And Distribution, Xiaohui Zhang, Miao Zhang, Endong Bao
Turkish Journal of Veterinary & Animal Sciences
Heat stress is a lethal threat to chickens. This study investigated the protective effect of a Ginkgo biloba extract EGB761 against heat stress in chickens in vivo. A total of 200 one-day-old hens were separated randomly into control (Con), heat stress (HS), 0.1% EGB administration and heat stress (0.1% EGB+HS), 0.3% EGB administration and heat stress (0.3% EGB+HS), and 0.6% EGB administration and heat stress (0.6% EGB+HS) groups. After EGB761 administration for 45 days, ten chickens were selected randomly, assigned to one of the designated groups, and exposed to a one-time heat stress condition of 38 ± 1 °C for …
Humoral Immunity Provided By A Novel Infectious Bronchitis Vaccine Supplemented Bybacterium-Like Particles (Blps), Emadoddin Tohidi, Abolfazl Ghaniei, Hamid Farzin, Alireza Haghparast
Humoral Immunity Provided By A Novel Infectious Bronchitis Vaccine Supplemented Bybacterium-Like Particles (Blps), Emadoddin Tohidi, Abolfazl Ghaniei, Hamid Farzin, Alireza Haghparast
Turkish Journal of Veterinary & Animal Sciences
Infectious bronchitis (IB) is a notable disease of poultry flocks that results in economic loss. As a consequence of the presence of various IB virus (IBV) serotypes, control strategies, such as vaccination, should be replaced by provide broad protective immunity against the disease to date. Gram-positive enhancer matrix particles, or so-called bacterium like particles (BLPs), obtained from the bacterium Lactococcus lactis (L. lactis), have demonstrated an adjuvancy effect by providing demanding mucosal and humoral immune responses, as well as a protective cellular immunity whenever delivered admixed with a vaccine via intranasal or intraocular administration. In this study, for the first …
Effect Of In-Bag Carcass Decontamination Method On Shelf Life Of Whole Chicken Carcassespackaged In Plastic Bags, İbrahi̇m Alper Uysal, Gökhan Kürşad İnci̇li̇, Ömer Çakmak, Mehmet Çalicioğlu
Effect Of In-Bag Carcass Decontamination Method On Shelf Life Of Whole Chicken Carcassespackaged In Plastic Bags, İbrahi̇m Alper Uysal, Gökhan Kürşad İnci̇li̇, Ömer Çakmak, Mehmet Çalicioğlu
Turkish Journal of Veterinary & Animal Sciences
This study was undertaken to investigate the changes in the microbiological and sensorial attributes of fresh chicken carcasses immersed in decontamination solutions, followed by packaging in plastic bags in which decontamination solution was added during storage at 4 °C. The samples were taken on days 0, 3, 7, and 10 for microbiological and sensory attributes. Results showed that there was a limited antimicrobial effect on microbial flora in the treatment groups within the first 3 days of storage compared to the control group. However, the difference between the groups completely disappeared on days 7 and 10. The changes in the …
Evaluation Of The In Vivo Efficacy Of Pumpkin (Cucurbita Pepo) Seeds Against Gastrointestinal Helminths Of Chickens, Jezie A. Acorda, Imma Ysabela Emille C. Mangubat, Billy P. Divina
Evaluation Of The In Vivo Efficacy Of Pumpkin (Cucurbita Pepo) Seeds Against Gastrointestinal Helminths Of Chickens, Jezie A. Acorda, Imma Ysabela Emille C. Mangubat, Billy P. Divina
Turkish Journal of Veterinary & Animal Sciences
The present study was conducted to evaluate the in vivo efficacy of pumpkin seeds as an alternative natural anthelmintic for chickens. Ninety Philippine Jolo native chickens of mixed sexes, aged 4?5 months and weighing 1?2 kg, were randomly distributed into three treatment groups with 30 chickens per group. Control group A was fed basic mash feed, group B received feed mixed with ground pumpkin seeds (2 g/bird per day), and group C received mebendazole-medicated feed (30 mg/kg body weight). Fifteen randomly selected chickens from each group were euthanized and necropsied before treatment, and the remaining fifteen in each group were …
Genetic Analysis Of Iranian Mycoplasma Gallisepticum Isolates Using Gene-Targeted Sequencing Of Complete Pvpa Gene, Naghmeh Kabiri, Mohsen Bashashati, Mehdi Vasfi Marandi, Mansour Banani, Fereshteh Sabouri
Genetic Analysis Of Iranian Mycoplasma Gallisepticum Isolates Using Gene-Targeted Sequencing Of Complete Pvpa Gene, Naghmeh Kabiri, Mohsen Bashashati, Mehdi Vasfi Marandi, Mansour Banani, Fereshteh Sabouri
Turkish Journal of Veterinary & Animal Sciences
This study aims to evaluate Mycoplasma gallisepticum (MG) genetic diversity through the complete sequencing of the pvpA gene among six MG isolates present in industrial poultry from 2015 to 2016. The genetic comparison of these six isolates showed similarities of over 94% and 92% in terms of nucleotide and amino acid sequences, respectively. The size of the PvpA protein in the studied isolates included three different sizes with 361, 364, and 366 amino acids. The carboxy-terminus of the studied PvpA proteins contained 11 tetrapeptides of P-R-P-M/Q/N motifs with truncation in the proline-rich region. Based on the BLAST analysis, the pvpA …
Intestinal Barrier And Mucosal Immunity In Broilers, Thai Betong, And Native Thai Praduhangdum Chickens, Sirin Theerawatanasirikul, Nunyarat Koomkrong, Autchara Kayan, Chaiwat Boonkaewwan
Intestinal Barrier And Mucosal Immunity In Broilers, Thai Betong, And Native Thai Praduhangdum Chickens, Sirin Theerawatanasirikul, Nunyarat Koomkrong, Autchara Kayan, Chaiwat Boonkaewwan
Turkish Journal of Veterinary & Animal Sciences
The intestinal barrier includes physical and chemical components for preventing the invasion of pathogenic and toxic agents. The aim of this study was to investigate small intestinal morphology, antimicrobial peptides, and tight junction (TJ) distribution among broilers, Thai Betong chickens, and native Thai Praduhangdum chickens. Intestinal samples from 40 chickens of each breed were collected. The results of the histological and morphological examination revealed that the duodenum of all breeds had the maximum villus height compared to the other parts. The intestinal tract of both Betong and Praduhangdum chickens was low in the number of mucin and goblet cells. In …
The Protective Efficacy Of Immunoglobulin Y From Immunized Chickens Against Salmonella Infections In Mice, Hasan Hüseyi̇n Hadi̇mli̇, Zafer Sayin, Gökçenur Sani̇oğlu Gölen
The Protective Efficacy Of Immunoglobulin Y From Immunized Chickens Against Salmonella Infections In Mice, Hasan Hüseyi̇n Hadi̇mli̇, Zafer Sayin, Gökçenur Sani̇oğlu Gölen
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine the efficacy of immunoglobulin Y (IgY) obtained from chickens immunized with Salmonella vaccines. Chickens were vaccinated three times with inactivated monovalent, bivalent, and combined vaccines. Immunized hen eggs were collected after the third vaccination and IgYs were purified. In total, 100 mice were orally challenged with Salmonella serotypes. After the challenge, IgYs were orally administered to mice. Mice were observed for morbidity and mortality. Fecal samples from the mice were also cultured for the reisolation of Salmonella serotypes. The antibody titers in the serum samples of vaccinated chickens were higher than those …
Pharmacokinetics And Bioavailability Of Three Promising Tilmicosin-Loaded Lipid Nanoparticles In Comparison With Tilmicosin Phosphate Following Oral Administration In Broiler Chickens, Ali Rassouli, Alwan Al-Qushawi, Fatemeh Atyabi, Seyed Mostafa Peighambari, Mehdi Esfandyari-Manesh, Gholam Reza Shams
Pharmacokinetics And Bioavailability Of Three Promising Tilmicosin-Loaded Lipid Nanoparticles In Comparison With Tilmicosin Phosphate Following Oral Administration In Broiler Chickens, Ali Rassouli, Alwan Al-Qushawi, Fatemeh Atyabi, Seyed Mostafa Peighambari, Mehdi Esfandyari-Manesh, Gholam Reza Shams
Turkish Journal of Veterinary & Animal Sciences
Tilmicosin (TLM) is a semisynthetic antimicrobial agent used mainly in poultry and cattle, but it has relatively poor oral bioavailability. This study was conducted to compare the bioavailability (BA) and main pharmacokinetic (PK) parameters of TLM after oral administration of tilmicosin phosphate (TLM-PH) and three newly prepared lipid nanoparticles (LNPs) of TLM including solid lipid nanoparticles (SLNs), nanostructured lipid carriers (NLCs), and lipid-core nanocapsules (LNCs). Sixty broiler chickens were divided into eight groups. In four treatment groups (n = 10), each bird was given a single oral dose (20 mg/kg) of a TLM formulation after overnight fasting, and in four …
A Novel Approach For Determination Of Chicken Sexing At An Early Stage Of Development By Using Loop-Mediated Isothermal Amplification Method, Sung Woo Kim, Jin Seok Choi, Neelesh Sharma, Yeoung Gyu Ko, Yoon Jung Do, Mijeong Byun, Hwan Hoo Seong, Soo Bong Park, Dong Kee Jeong
A Novel Approach For Determination Of Chicken Sexing At An Early Stage Of Development By Using Loop-Mediated Isothermal Amplification Method, Sung Woo Kim, Jin Seok Choi, Neelesh Sharma, Yeoung Gyu Ko, Yoon Jung Do, Mijeong Byun, Hwan Hoo Seong, Soo Bong Park, Dong Kee Jeong
Turkish Journal of Veterinary & Animal Sciences
The monomorphism of newly hatched chicks is a major hurdle for poultry farmers at the time of sexing. The traditional sexing techniques are inaccurate and time-consuming. Therefore, the present study aimed to develop a loop-mediated isothermal amplification (LAMP)-based sex determination method to obtain a higher separation accuracy rate as compared to conventional methods. LAMP is a simple, quick, and relatively inexpensive diagnostic tool. Genomic DNA was extracted from the blood samples of Leghorn and Korean native chicks for LAMP. Two sets of primer were designed, consisting of four isothermal amplification primers in each, to amplify the female sex-specific sequence (HUR0417) …
Study On The Relationship Between Single Nucleotide Polymorphisms Of The Coccidiosis-Resistance Candidate Zyxin Gene Exon 1 And Carcass Traits In The Jinghai Yellow Chicken, Guojun Dai, Dahui Sun, Mingming Sun, Yuxin Lin, Xiang Wang, Genxi Zhang, Kaizhou Xie, Miaomiao Shi, Olajide Olowofeso, Jinyu Wang
Study On The Relationship Between Single Nucleotide Polymorphisms Of The Coccidiosis-Resistance Candidate Zyxin Gene Exon 1 And Carcass Traits In The Jinghai Yellow Chicken, Guojun Dai, Dahui Sun, Mingming Sun, Yuxin Lin, Xiang Wang, Genxi Zhang, Kaizhou Xie, Miaomiao Shi, Olajide Olowofeso, Jinyu Wang
Turkish Journal of Veterinary & Animal Sciences
Previous research revealed that several single nucleotide polymorphisms (SNPs) of zyxin were significantly associated with coccidiosis-resistance parameters. In this research, the Jinghai yellow chicken was chosen to detect the SNPs of zyxin gene exon 1 by PCR-SSCP and DNA sequencing techniques, and was also used to analyze the effect of zyxin SNPs on carcass traits. A single mutation was found with 3 genotypes, designated as AA, AB, and BB. The results showed that BB was significantly different from AA and AB in terms of abdominal fat weight and slaughter rate for cockerel (P < 0.05), while there was no significant difference between AA and AB (P > 0.05). The heart weight, breast muscle rate, …
Growth Performance And Intestinal Histomorphology In Egg-Type Growing Roosters Fed Recycled Food Waste Containing Effective Microorganisms, Jassada Ruttanavut, Koh En Yamauchi
Growth Performance And Intestinal Histomorphology In Egg-Type Growing Roosters Fed Recycled Food Waste Containing Effective Microorganisms, Jassada Ruttanavut, Koh En Yamauchi
Turkish Journal of Veterinary & Animal Sciences
To evaluate the effects of a recycled food waste (eco-feed) containing effective microorganisms derived from Japanese mugwort silage juice (EJP) on growth performance and intestinal histomorphology, 48 male chickens of 14 days old were divided into 0%, 20%, 40%, and 60% EJP diet groups. At 20 weeks of age, body weight gain was similar in the 20% EJP group but lower in the 40% and 60% EJP groups (P < 0.05) compared to that in the control group. The relative total intestinal length and weight tended to be long and heavy, respectively, in the 20% and 40% EJP groups, but short and light, respectively, in the 60% EJP group. Although numerous villi were found in the jejunum and ileum of all EJP groups, the villus height and size observed in these groups were smaller. Cell area and cell mitosis number in the duodenum and jejunum of all EJP groups were lower than those of the control group (P < 0.01). Epithelial cells on the villus apical surface of the 20% EJP group showed morphology similar to that of cells on the villus apical surface of the control group in all of the intestinal segments. The growth performance data suggested that EJP could be incorporated at up to a level of 20% in chicken diets. The intestinal histological results suggested that long-term feeding of an EJP diet at up to the 40% level may result in a long and heavy intestine by increasing the numbers of miniature intestinal villi.
Phenotypic Characterisation Of Enterococcus Spp. From Femoral Head Necrosis Lesions Of Chickens, Nevi̇n Oktay, Seran Temelli̇, K. Tayfun Çarli
Phenotypic Characterisation Of Enterococcus Spp. From Femoral Head Necrosis Lesions Of Chickens, Nevi̇n Oktay, Seran Temelli̇, K. Tayfun Çarli
Turkish Journal of Veterinary & Animal Sciences
Two types of Enterococcus species were isolated from femoral head necrosis lesions of chickens. A total of 150 femoral articular tissues, of which 121 were from commercial broilers, 18 from broiler breeders, and 11 from layer breeders, were sampled. Out of 48 Enterococcus isolates of these clinical samples, 37 and 11 isolates were identified as Enterococcus faecalis and Enterococcus faecium, respectively. Additionally, 42 (28%) Escherichia coli and 33 (22%) Staphylococcus aureus isolates were obtained either alone or together with 1 of the 2 Enterococcus species from the same clinical samples, while no bacteria were detected from the remaining 56 samples. …
Single Strand Conformation Polymorphism In Intron I Of The Chicken Apovldl-Ii Gene, And Its Relationship With Triglyceride And Very Low Density Lipoprotein Concentrations, Hassan Hussein Musa, Guo H. Chen, Jin H. Cheng, Wen B. Bao, Adam H. Suleiman
Single Strand Conformation Polymorphism In Intron I Of The Chicken Apovldl-Ii Gene, And Its Relationship With Triglyceride And Very Low Density Lipoprotein Concentrations, Hassan Hussein Musa, Guo H. Chen, Jin H. Cheng, Wen B. Bao, Adam H. Suleiman
Turkish Journal of Veterinary & Animal Sciences
Genetically fat and lean chickens reared identically in the same environment were used to investigate the effect of the apoVLDL-II gene (intron I) on total cholesterol, triglyceride, and lipoprotein concentrations. The overall allele frequency in the 2 populations was 0.59 for allele A and 0.41 for allele B. The allele frequency in the Rugao breed was 0.52 for allele A and 0.48 for allele B; however, in the Anka breed it was 0.65 for allele A and 0.35 for allele B. The apoVLDL-II genotype in the genetically fat Anka breed was associated with a significant increase in serum triacylglyceride concentration …
Pathological And Biochemical Effects Of Intramuscular Gentamicin Administration In Chickens, Ijaz Khan, Muhammad Zargham Khan, Muhammad Kashif Saleemi, Ijaz Javed, Ahrar Khan
Pathological And Biochemical Effects Of Intramuscular Gentamicin Administration In Chickens, Ijaz Khan, Muhammad Zargham Khan, Muhammad Kashif Saleemi, Ijaz Javed, Ahrar Khan
Turkish Journal of Veterinary & Animal Sciences
The objective of this experimental study was to examine the pathological effects of the intramuscular administration of gentamicin to growing chickens. Two experiments of 35 days´ duration were conducted simultaneously. In both experiments White Leghorn cockerels of 6 weeks of age were randomly divided into 4 groups and gentamicin was administered intramuscularly to the birds on days 1 and 3 at a dose of 10, 50, 100, and 0 mg/kg body weight, respectively. Birds administered 50 and 100 mg/kg gentamicin showed depression, diarrhea, increased water intake, and lower body weights. Mortality was 0%, 26%, and 100% in the 10, 50, …
Application Of Mentofin^® In Broilers With Clinical Infectious Bursal Disease To Reduce Escherichia Coli Related Problems After Vaccination Against Newcastle Disease, K. Tayfun Çarli, Kaan Önat, Elçi̇n Günaydin
Application Of Mentofin^® In Broilers With Clinical Infectious Bursal Disease To Reduce Escherichia Coli Related Problems After Vaccination Against Newcastle Disease, K. Tayfun Çarli, Kaan Önat, Elçi̇n Günaydin
Turkish Journal of Veterinary & Animal Sciences
In this study Mentofin^® was used in commercial broiler chickens naturally exposed to infectious bursal disease virus (IBDV) and administered live Newcastle disease virus (NDV) vaccine to evaluate the ability of Mentofin^® to reduce Escherichia coli-related respiratory lesions, immunomodulate Newcastle disease (ND) vaccine response, change pharyngeal aerobic bacterial counts, and have certain impacts on specific production parameters. Mentofin^® was added to the drinking water (100 ml/500 l water) of broiler chickens aged between 3 and 5 days. ND and IBD vaccinations were administered via drinking water to 15-day-old chickens. NDV vaccination was given again when the chickens were 20 days …
Investigation Of Chicken Infectious Anemia Virus Infection By Pcr And Elisa In Chicken Flocks, H. Hüseyi̇n Hadi̇mli̇, Osman Ergani̇ş, Leyla Güler, Uçkun Sai̇t Uçan
Investigation Of Chicken Infectious Anemia Virus Infection By Pcr And Elisa In Chicken Flocks, H. Hüseyi̇n Hadi̇mli̇, Osman Ergani̇ş, Leyla Güler, Uçkun Sai̇t Uçan
Turkish Journal of Veterinary & Animal Sciences
Presence of the chicken infectious anemia virus (CIAV) infection in chicken flocks in some provinces of Turkey was investigated by ELISA and PCR in this study. From 38 flocks (16 commercial layer, 10 commercial broilers, 4 breeder layers and 8 breeder broilers), 922 serum samples were tested for CIAV-specific antibodies using a commercially available competitive ELISA. CIAV antibodies were positive in 609 (66.0%) sera from 34 flocks (89.5%). A total of 95 thymus samples from 25 flocks, 57 samples from 15 commercial layers, and 38 samples from 10 commercial broiler flocks were tested by PCR. In 53 (55.8%) thymus samples …
Associations Between Ghr And Igf-1 Gene Polymorphisms, And Reproductive Traits In Wenchang Chickens, Hui Fang Li, Wen Qi Zhu, Kuan Wei Chen, Xu Wu, Qing Ping Tang, Yu Shi Gao
Associations Between Ghr And Igf-1 Gene Polymorphisms, And Reproductive Traits In Wenchang Chickens, Hui Fang Li, Wen Qi Zhu, Kuan Wei Chen, Xu Wu, Qing Ping Tang, Yu Shi Gao
Turkish Journal of Veterinary & Animal Sciences
Alleles of physiological candidate genes for reproductive traits, insulin-like growth factor-1 (IGF-1), and growth hormone receptor (GHR) were assessed to determine their associations with total egg production (NE), average days of continual egg-laying (ADCE), and number of double yolk eggs (DYE) in Wenchang chickens (Chinese indigenous breed). PCR-RFLP was used for genotype identification. The frequency of restriction enzyme C1/C2 alleles in the population was 0.53 (C1) and 0.47 (C2) for IGF-1. For GHR-intron 2 it was 0.06 and 0.94 for (A1) and (A2), respectively, while for GHR-intron 5 it was 0.20 (B1) and 0.80 (B2). Four significant associations were found …
Immunomodulatory And Protective Effects Of Sugar Cane Juice In Chickens Against Eimeria Infection, Masood Akhtar, M. Abdul Hafeez, Faqir Muhammad, Ahsan Ul Haq, M. Irfan Anwar
Immunomodulatory And Protective Effects Of Sugar Cane Juice In Chickens Against Eimeria Infection, Masood Akhtar, M. Abdul Hafeez, Faqir Muhammad, Ahsan Ul Haq, M. Irfan Anwar
Turkish Journal of Veterinary & Animal Sciences
Present paper reports the effects of sugar cane juice (SCJ) on immune responses and its protection against Eimeria (mixed species) infections in broiler chickens. Experiment 1 was conducted to test the dose efficacy of SCJ and its effects on chickens against coccidiosis. Maximum weight gain per day was recorded in chickens given SCJ @ 400 mg/kg body weight. Significantly (P < 0.01) low mortality and increased body weight gain per day were recorded in chickens administered SCJ orally and challenged with mixed species of genus Eimeria. Furthermore, mild hemorrhages, lower lesion scores (+1), and significantly lower number of oocysts shed in faeces were recorded in immunized chickens compared to control. Experiment 2 was carried out to evaluate the immunomodulatory effects of SCJ on cellular and humoral responses. Highest antibody titres (1:1024-1:16; GMT 367.28) were recorded in chickens administered SCJ @ 400 mg/kg body weight in comparison with control (1:16-1:2; GMT 2.82). To demonstrate the cell mediated immunity (CMI), amplitude of toe-web swelling 72 h post avian tuberculin injection was recorded in the experimental and control groups. Maximum swelling (8.13 ± 0.21 mm) was recorded in the experimental group as compared to control (1.04 ± 0.10); indicating higher cell mediated immune response. From these results it was concluded that SCJ elicit a significantly higher cellular and humoral responses compared to control, which may provide protection against Eimeria infections (mixed species) in chickens.
Detection Of The Eaea Gene In Escherichia Coli From Chickens By Polymerase Chain Reaction, Ayşe Kiliç, Hasan Basri̇ Ertaş, Adi̇le Muz, Gökben Özbey, Hakan Kalender
Detection Of The Eaea Gene In Escherichia Coli From Chickens By Polymerase Chain Reaction, Ayşe Kiliç, Hasan Basri̇ Ertaş, Adi̇le Muz, Gökben Özbey, Hakan Kalender
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to isolate Escherichia coli from chickens and to determine the presence of the eaeA gene, a virulence factor detected in E. coli, in the isolates by polymerase chain reaction (PCR). Different chicken organs were inoculated onto blood agar and biochemical tests were performed on the suspicious isolates. E. coli was isolated from 48% (48/100) of the samples. DNA was extracted from these isolates and was amplified by PCR, using a pair of primers derived from the eaeA (virulence) gene. In the agarose gel examination of PCR products, 48% (48/100) of the isolates were determined …
Relation Between Abdominal Fat And Serum Cholesterol, Triglycerides, And Lipoprotein Concentrations In Chicken Breeds, Hassan Hussein Musa, Guo H. Chen, Jin H. Cheng, Galal M. Yousif
Relation Between Abdominal Fat And Serum Cholesterol, Triglycerides, And Lipoprotein Concentrations In Chicken Breeds, Hassan Hussein Musa, Guo H. Chen, Jin H. Cheng, Galal M. Yousif
Turkish Journal of Veterinary & Animal Sciences
This study was undertaken to investigate the relation between abdominal fat and serum biochemical indices in chicken. In the study, 120 chickens at 12 weeks of age from Anka and Rugao breeds were used. They were reared under the same environment and management. Blood samples were taken, the serum was harvested by centrifugation, and then the total cholesterol (TCH), triglycerides (TG), and high-density lipoprotein (HDL) were assayed using an enzymatic kit. Very low-density lipoprotein (VLDL) and low-density lipoprotein (LDL) were determined using the Friedwald equation. Chickens were slaughtered and the carcasses were dissected manually to estimate the abdominal fat weight. …
The Prevalence Of Campylobacter Jejuni In Various Sources In Kayseri, Turkey, And Molecular Analysis Of Isolated Strains By Pcr-Rflp, Fuat Aydin, K. Semi̇h Gümüşsoy, Tuba İça, Bülent Sümerkan, Duygu Eşel, Mehmet Akan, Ayşe Özdemi̇r
The Prevalence Of Campylobacter Jejuni In Various Sources In Kayseri, Turkey, And Molecular Analysis Of Isolated Strains By Pcr-Rflp, Fuat Aydin, K. Semi̇h Gümüşsoy, Tuba İça, Bülent Sümerkan, Duygu Eşel, Mehmet Akan, Ayşe Özdemi̇r
Turkish Journal of Veterinary & Animal Sciences
The objective of this study was to isolate, identify, and genotype Campylobacter jejuni from various sources in the province of Kayseri, Turkey. A total of 6667 samples consisting of 5167 human fecal swabs, 600 dog rectal swabs, 600 cattle gallbladders, and 300 chicken carcasses were examined. The samples were plated onto mCCDA (cefoperazone charcoal desoxycholate agar) agar. In order to identify C. jejuni, phenotypic tests and PCR (polymerase chain reaction) were performed. C. jejuni was isolated in 1.43%, 43.50%, 31.16%, and 56% of the human, dog, cattle, and chicken samples, respectively. Among the 690 C. jejuni strains that were isolated …
Immunohistochemical Expression Of Estrogen And Progesterone Receptors In Oviduct Adenocarcinomas In Laying Hens, M. Özgür Özyi̇ği̇t, Gürsel Sönmez
Immunohistochemical Expression Of Estrogen And Progesterone Receptors In Oviduct Adenocarcinomas In Laying Hens, M. Özgür Özyi̇ği̇t, Gürsel Sönmez
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to demonstrate the presence of estrogen and progesterone receptors (ERs and PRs, respectively) in oviduct adenocarcinomas in laying hens. In all, 32 oviduct adenocarcinomas (18 primary tumors and 14 intestinal metastases) from Babcock B-380 laying hens 16-18 months old were stained with commercially available monoclonal antibodies against mouse-anti-human ERs and mouse-anti-human PRs. In the microscopic examination, 13 tumors (7 oviduct adenocarcinomas and 6 intestinal metastases) stained positively for ERs and 12 tumors (4 oviduct adenocarcinomas and 8 intestinal metastases) gave a positive reaction to PR antibody. The staining pattern of the tumor cells with …
Evaluation Of Two Vaccination Schemes Using Live Vaccines Against Newcastle Disease In Chickens, Mansur-Ud-Din Ahmad, Mamoona Chaudhry, Muhammad Farooq Rai, Hamad Bin Rashid
Evaluation Of Two Vaccination Schemes Using Live Vaccines Against Newcastle Disease In Chickens, Mansur-Ud-Din Ahmad, Mamoona Chaudhry, Muhammad Farooq Rai, Hamad Bin Rashid
Turkish Journal of Veterinary & Animal Sciences
The use of different types of Newcastle disease (ND) vaccine in different vaccination schemes has decreased the incidence of velogenic Newcastle disease (VND) in commercial poultry worldwide. In under-developed countries like Pakistan, these vaccination schemes are not successful due to free-range and backyard poultry production. This trial was conducted in 90 experimental chickens to develop an effective control against ND. The level of antibody response, detected by haemagglutination-inhibition (HI) test, and the degree of protection against the virulent strain of Newcastle disease virus (NDV) were studied. The chickens were immunized with commercially available ND vaccines. In scheme A, primary vaccination …
In Vitro Effects Of Some Antibiotics On Glutathione Reductase Obtained From Chicken Liver, Hülya Demi̇r, Mustafa Erat, Hali̇s Şaki̇roğlu
In Vitro Effects Of Some Antibiotics On Glutathione Reductase Obtained From Chicken Liver, Hülya Demi̇r, Mustafa Erat, Hali̇s Şaki̇roğlu
Turkish Journal of Veterinary & Animal Sciences
Glutathione reductase (GR) is important in maintaining high levels of reduced glutathione in cells. The enzyme is inhibited by some drugs used in antimicrobial chemotherapy. The effects of some antibiotics on GR activity were investigated in this study. At first, the enzyme, having a specific activity of 88.28 EU/mg proteins, was purified 1636-fold with a yield of 35%. Purity of the enzyme was checked by means of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Activity of the enzyme was determined spectrophotometrically at 340 nm and in vitro effects of ofloxacin, levofloxacin, cefazolin, and cefepime were determined. The antibiotics used in …
Alterations To Lipid Parameters In Response To Fig Tree (Ficus Carica) Leaf Extract In Chicken Liver Slices, Farzad Asadi, Malihe Pourkabir, Robin Maclaren, Ali Shahriari
Alterations To Lipid Parameters In Response To Fig Tree (Ficus Carica) Leaf Extract In Chicken Liver Slices, Farzad Asadi, Malihe Pourkabir, Robin Maclaren, Ali Shahriari
Turkish Journal of Veterinary & Animal Sciences
Lipid storage, particularly in the abdominal cavity, is a major concern in poultry breeding because it affects the net meat yield. The major contributors to lipid storage in the abdominal cavity are hepatic fatty acid synthesis and VLDL secretion. These factors are strongly correlated with the body fatness in the chicken. The aim of our study was to determine if a traditional food supplement, the extract from fig tree leaves (fig tree leaf extract (FTE)), can be used to decrease hepatic triglyceride (TG) content and secretion of TG and cholesterol (TC) from the liver. Livers from 8-week-old roosters (n = …
The Histochemical And Ultrastructural Structures Of Avian Latissimus Dorsi Muscle Fiber Types And Changes In Them Caused By Water Copper Level, Fati̇me Geyi̇koğlu, Özgen Vuraler, Aysel Temelli̇
The Histochemical And Ultrastructural Structures Of Avian Latissimus Dorsi Muscle Fiber Types And Changes In Them Caused By Water Copper Level, Fati̇me Geyi̇koğlu, Özgen Vuraler, Aysel Temelli̇
Turkish Journal of Veterinary & Animal Sciences
The histochemical and ultrastructural effects of water Cu on the anterior latissimus dorsi (ALD) muscle of 120 newly hatched broilers were investigated in a 45-day experiment. Two doses of water Cu (250 mg Cu/kg water and 500 mg Cu/kg water) were given to the poults. Two distinct categories (IIIA and IIIB) of slow tonic fibers in the control groups were detected by enzyme reactions. The ultrastructure of the Z-line, mitochondrial content and sarcotubular content differed between the 2 fiber types. In the application of 250 mg Cu/kg water, the histochemical characteristics of the IIIB fibers were clearly different from those …
Isolation Of Clostridium Perfringens From Chickens And Detection Of The Alpha Toxin Gene By Polymerase Chain Reaction (Pcr), Hakan Kalender, Hasan Basri̇ Ertaş
Isolation Of Clostridium Perfringens From Chickens And Detection Of The Alpha Toxin Gene By Polymerase Chain Reaction (Pcr), Hakan Kalender, Hasan Basri̇ Ertaş
Turkish Journal of Veterinary & Animal Sciences
This study was carried out to isolate Clostridium perfringens from chickens and to detect the gene encoding the alpha toxin produced by all types of C. perfringens by polymerase chain reaction (PCR). Intestinal contents of 160 slaughtered chickens from 8 different farms in Elazığ province were analyzed. C. perfringens was isolated from 8 (5%) of the samples. DNA samples extracted from suspected isolates grown on selective agar were amplified by PCR using a pair of primers derived from the alpha toxin gene. All of 8 suspected isolates were found to be C. perfringens by conventional methods and PCR. Isolates were …
Ornithobacterium Rhinotrachelae (Or) Infection, Nesri̇n Turan, Seyyal Ak
Ornithobacterium Rhinotrachelae (Or) Infection, Nesri̇n Turan, Seyyal Ak
Turkish Journal of Veterinary & Animal Sciences
Ornithobacterium rhinotracheale infection is characterized by respiratory disease, decreased growth and increased mortality in avians. Although a reasonably new infection, it has been found in many countries in a short period. In this article, this infection, which causes large financial losses in avian enterprises, was investigated.