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Investigation Of Bmp15 And Gdf9 Gene Polymorphisms And Their Effects On Litter Size In Anatolian Sheep Breed Akkaraman, Koray Kirikçi
Investigation Of Bmp15 And Gdf9 Gene Polymorphisms And Their Effects On Litter Size In Anatolian Sheep Breed Akkaraman, Koray Kirikçi
Turkish Journal of Veterinary & Animal Sciences
This study aimed to examine the polymorphism of the BMP15 (FecX$^{G}$ and FecX$^{I}$) and GDF9 (G1 and G4) genes and the association between genotypes and litter size in the Akkaraman breed. Blood samples were taken from 100 ewes in total. Genomic DNA was obtained from whole blood. The PCR-RFLP technique was employed to determine the polymorphisms of the genes under investigation. The G1 was the only polymorphic among the four loci analyzed. The frequencies for the GA and GG genotypes were 0.26 and 0.74, respectively, while the A and G allele frequencies were 0.13 and 0.87, respectively. The observed and …