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Full-Text Articles in Veterinary Medicine
Molecular Characterization And Expression Analysis Of Mir-29a In Porcine Cells And Porcine Reproductive And Respiratory Syndrome Virus Infected Peripheral Blood Mononuclear Cells, Win Surachetpong, Sunaree Nantakhruea, Porntippa Lekcharoensuk
Molecular Characterization And Expression Analysis Of Mir-29a In Porcine Cells And Porcine Reproductive And Respiratory Syndrome Virus Infected Peripheral Blood Mononuclear Cells, Win Surachetpong, Sunaree Nantakhruea, Porntippa Lekcharoensuk
The Thai Journal of Veterinary Medicine
MicroRNAs are a group of small non-coding RNA that are involved in posttranscriptional regulation through target mRNA degradation or inhibition of protein synthesis. The objective of this present study was to characterize and determine expression level of porcine miR-29a in various tissues. Our results indicated that porcine miR-29a shared high conserved sequence with miR-29a from other organisms. Furthermore, in silico analysis suggested that the seed region of porcine miR-29a partially matched IFN-γ mRNA. RT-PCR analysis indicated that miR-29a expressed in various tissues including lymph node, spleen, peripheral blood mononuclear cells (PBMCs) and PK-15 cells. Expression profiling of miR-29a in PRRSV-infected …
Analysis Of Mirna-29a Expression In Porcine Peripheral Blood Mononuclear Cells Using Quantitative Reverse Transcription Polymerase Chain Reaction, Sunaree Nantakhruea, Porntippa Lekcharoensuk, Win Surachetpong
Analysis Of Mirna-29a Expression In Porcine Peripheral Blood Mononuclear Cells Using Quantitative Reverse Transcription Polymerase Chain Reaction, Sunaree Nantakhruea, Porntippa Lekcharoensuk, Win Surachetpong
The Thai Journal of Veterinary Medicine
MicroRNAs (miRNAs) are small non coding RNA that regulate gene expression as post transcriptional regulators in mammalian tissues. One of the miRNAs that participates in mammalian immune response is miR-29a. Despite the limited information of miR-29a in porcine cells, it is interesting to determine whether miR-29a involves in immune regulation in pig. In this study, we developed a quantitative reverse transcription polymerase chain reaction using SYBR detection system to examine the expression of primary miR-29a (pri-miR-29a) in porcine peripheral blood mononuclear cells (PBMCs) in the presence of mitogen. PBMCs were stimulated with concanavalin A (Con A) at a concentration of …