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Articles 31 - 60 of 98
Full-Text Articles in Veterinary Medicine
Pharmacokinetics Of Micronized Progesterone Administration In Female Dogs, Raphael Anthony Malbrue
Pharmacokinetics Of Micronized Progesterone Administration In Female Dogs, Raphael Anthony Malbrue
LSU Master's Theses
Hypoluteoidism in the bitch is described as a reproductive condition in which insufficient levels of endogenous progesterone are present resulting in failure to maintain a functional secretory endometrium. This condition can prevent normal embryo implantation, development, and ultimately end in pregnancy loss. Hypoluteoidism in the bitch is a rising concern in small animal theriogenology and current medical therapies available to veterinarians are limited. The aim of this study was to determine the pharmacokinetics (PK) of intravaginally (Crinone®, Serono Laboratories, Norwell, MA) and orally delivered micronized progesterone (Prometrium®, Solvay Pharmaceuticals, Inc., Marietta, GA) in the bitch. We hypothesized that both vaginal …
Quantitation Of Anti-Pythium Insidiosum Antibodies Before And After Immunotherapy In Healthy Dogs, Carmen Beatriz Arsuaga
Quantitation Of Anti-Pythium Insidiosum Antibodies Before And After Immunotherapy In Healthy Dogs, Carmen Beatriz Arsuaga
LSU Master's Theses
Pythium insidiosum is an aquatic oomycete that causes invasive, progressive granulomatous lesions of the skin in dogs, horses, and cats, and of the gastrointestinal tract in dogs. Quantitation of anti-P. insidiosum IgG antibodies can be used in dogs to both confirm a suspected diagnosis and to monitor response to therapy. Recently, an immunotherapeutic product (IP) has been marketed for the treatment of pythiosis in dogs, horses, and people. The aim of this study was to evaluate the effect of administration of this product on anti-P. insidiosum IgG concentrations in dogs. The IP was administered to seven, healthy hound mixes on …
Development Of Mouse Models For The Study Of Zika Virus Pathogenesis And Antibody Response, Anna Beatriz Kawiecki
Development Of Mouse Models For The Study Of Zika Virus Pathogenesis And Antibody Response, Anna Beatriz Kawiecki
LSU Master's Theses
After the emergence of Zika virus (ZIKV) in the Americas in 2015, ZIKV infection was associated for the first time since its discovery with severe symptoms in both adults and congenital cases, including neurological, ocular, and developmental manifestations. Previous ZIKV circulation in Africa and Southeast Asia has been characterized by mild symptoms and small-scale case-counts. It is unclear whether the unprecedented size and severity of the ZIKV outbreak in the Americas are the consequence of a change in the virus, different background flaviviral immunity in the population, or a reporting issue. In addition, ZIKV has been shown to be transmitted …
Evaluation Of Broad Anti-Herpesviral Activity With Α-Hydroxytropolones, Shannon David Dehghanpir
Evaluation Of Broad Anti-Herpesviral Activity With Α-Hydroxytropolones, Shannon David Dehghanpir
LSU Master's Theses
Herpesviruses are ubiquitous in animals and cause economic losses concomitant with many diseases, including upper respiratory disease, keratitis, abortion, neonatal death, and neurologic disease. The majority of the domestic animal herpesviruses are within the subfamily Alphaherpesvirinae, along with the prototypical human herpes simplex virus 1 (HSV-1). Suppression of HSV-1 replication has been reported with α-hydroxytropolones (αHTs), which are aromatic ring compounds that have broad bioactivity due to potent chelating activity. It is postulated that αHTs inhibit enzymes within the nucleotidyltransferase superfamily (NTS), similarly structured enzymes that require divalent cations for nucleic acid cleavage activity. One potential herpesviral target includes the …
The Role Of Nadph Oxidase 2 (Nox2) In High-Fat Diet-Induced Adiposopathy And Brain Dysfunction In A Mouse Model, Jennifer Kathleen Pepping
The Role Of Nadph Oxidase 2 (Nox2) In High-Fat Diet-Induced Adiposopathy And Brain Dysfunction In A Mouse Model, Jennifer Kathleen Pepping
LSU Master's Theses
Obesity can have numerous detrimental consequences, namely metabolic syndrome, type 2 diabetes, cardiovascular disease, cancer, and Alzheimer’s disease. The pathogenesis and physiologic consequences of obesity are unknown, but they are often associated with increased inflammation and oxidative stress in both the body and in the brain. One factor that has been implicated in causing inflammation associated with a high fat diet is the enzyme NADPH oxidase, or NOX, specifically the subunit NOX2. Two studies were performed in order to assess the effects of a high fat diet in combination with a universal NOX2 deficiency and a NOX2 deficiency targeted to …
The Effects Of Prostatic Fluid On Functional Characteristics Of Cooled Canine Semen, Reto Fritsche
The Effects Of Prostatic Fluid On Functional Characteristics Of Cooled Canine Semen, Reto Fritsche
LSU Master's Theses
The objectives of this study were to investigate concentration dependent effects of canine prostatic fluid (PF) on in vitro seminal parameters of cooled canine semen. Sperm motility parameters, plasma membrane integrity and stability, acrosome integrity and DNA fragmentation were measured after the addition of 0%, 10%, 25%, or 50% PF to extended semen of fertile dogs. Assessments were made at 0 h pre-cooling, at 24, and 48 h of cooled storage (4 °C), and after freezing and thawing followed by incubation (37 °C) at 0, 4 and 24 h. Our hypothesis was that lower dilutions of canine semen with PF …
Correlation Of Ultrasonographic Small Intestinal Wall Layering With Histology In Normal Dogs, Alexandre Benjamin Le Roux
Correlation Of Ultrasonographic Small Intestinal Wall Layering With Histology In Normal Dogs, Alexandre Benjamin Le Roux
LSU Master's Theses
Five intestinal layers are commonly described ultrasonographically in dogs. However, current high-frequency endosonography allows the identification of 9 layers in people. The aim of this study was to describe ex vivo small intestinal layering in dogs and correlate ultrasonographic layering with histological layers. Our hypothesis was that, similar to findings in humans, discrepancies exist in thickness and visibility of intestinal layers between histology and ultrasound in dogs. Twelve adult dogs were included in the study. They were euthanized for reasons unrelated to gastrointestinal disease, but extensive medical history was unavailable. Duodenum, jejunum and ileum samples were resected immediately after euthanasia. …
The Role Of Monocyte Chemoattractant Protein-1 In Innate Immunity Against Methicillin Resistant Staphylococcus Aureus Pneumonia, Mary Katherine Leissinger
The Role Of Monocyte Chemoattractant Protein-1 In Innate Immunity Against Methicillin Resistant Staphylococcus Aureus Pneumonia, Mary Katherine Leissinger
LSU Master's Theses
Monocyte chemoattractant protein-1 is critical for monocyte recruitment to the lungs in response to bacterial infection. MCP-1 is also essential for protective neutrophil recruitment to the lungs during Escherichia coli and Klebsiella pneumoniae infection. Staphylococcus aureus pneumonia, specifically strain USA300, carries a high morbidity and mortality rate and is an important pathogen in hospital/ventilator and community acquired pneumonia. In the current study, we investigated the role of MCP-1 in pulmonary innate immunity to S. aureus in C57Bl/6, MCP-1-/- and MCP-1 AB blocked mice. As compared to C57Bl/6, MCP-1-/- mice showed increased concentrations of neutrophils in the airways and lung parenchyma …
Cardiovascular Tolerance And Safety Of Intravenous Lidocaine In The Broiler Chicken (Gallus Gallus Domesticus), Joao Manuel Lemos Brandao
Cardiovascular Tolerance And Safety Of Intravenous Lidocaine In The Broiler Chicken (Gallus Gallus Domesticus), Joao Manuel Lemos Brandao
LSU Master's Theses
Lidocaine, an amide local anesthetic agent, is commonly used in mammals, including humans. There is a general assumption that birds are more sensitive to lidocaine than mammals. Relatively low doses of lidocaine have been suggested to cause toxic effects in birds. While this information appears to be anecdotal, it has been perpetuated in the literature. The overall objective of this thesis research was to determine the tolerance and safety of intravenous lidocaine in broiler chickens. To assess the cardiovascular effects of lidocaine, relative changes on heart rate and mean blood pressure were calculated. Clinically significant cardiovascular effects were defined as …
Autologous Adipose Derived Adult Multipotent Stromal Cells Alter The Porcine Systemic Immune And Bone Biomarkers Response To Cancellous Bone Xenografts, Jonathan Francis Bova
Autologous Adipose Derived Adult Multipotent Stromal Cells Alter The Porcine Systemic Immune And Bone Biomarkers Response To Cancellous Bone Xenografts, Jonathan Francis Bova
LSU Master's Theses
The use of a porcine model in assessing bone grafts in vivo is common when a large animal model is necessary. In this thesis we aimed to improve the porcine model of facial reconstruction through the use of a local anesthesia and novel methods of assessing the immune response to and bone forming ability of adult adipose derived mesenchymal stromal cells (ASC). The goals of the research were: 1) evaluate the effect of a bupivacaine mandibular nerve block on blood pressure (BP) and heart rate (HR) in response to surgical stimulation and the need for systemic analgesics postoperatively, 2) quantify …
Characterization Of Ige-Mediated Cutaneous Immediate And Late-Phase Reactions In Non-Allergic Horses, Michelle Woodward
Characterization Of Ige-Mediated Cutaneous Immediate And Late-Phase Reactions In Non-Allergic Horses, Michelle Woodward
LSU Master's Theses
ABSTRACT Objectives – To characterize the response of horse skin following intradermal injection of polyclonal rabbit anti-canine IgE (anti-IgE) and rabbit immunoglobulin G (IgG) in an attempt to develop a model of equine allergic skin disease. Study design - In vivo study. Animals – 10 Adult Thoroughbred horses. Methods –Horses were injected intradermally with one of two different concentrations of anti-IgE and rabbit immunoglobulin G (IgG). Wheal measurements and injection site biopsies were obtained before and 20 min, 6 hr, 24 hr, and 48 hr after injection. Tissue sections were stained with Hematoxylin and Eosin, Luna, and Toluidine Blue. Immunohistochemistry …
Computed Tomographic Assessment Of Canine Arytenoid Lateralization, James Richard Wignall
Computed Tomographic Assessment Of Canine Arytenoid Lateralization, James Richard Wignall
LSU Master's Theses
Unilateral arytenoid lateralization is a commonly performed surgical treatment for laryngeal paralysis in dogs. It involves fixing the moveable arytenoid cartilage to the thyroid (TAL) or cricoid (CAL) cartilage or both (CTAL). This increases the area of the rima glottidis (RGA), to allow reduced airway pressure and laryngeal resistance in vitro and ameliorates clinical signs in vivo. It may also increase the patient’s predisposition for aspiration pneumonia, which occurs in around 20% of clinical patients. No surgical technique has been correlated with clinical outcome or risk of aspiration pneumonia. Objective analysis of the effects of surgery on the three dimensional …
Copper Oxide Wire Particles Used To Control Haemonchus Infections : Efficacy In Giraffe (Giraffa Camelopardalis) At Busch Gardens Tampa And Potential Mechanism Of Action, Allyson Kinney Moscona
Copper Oxide Wire Particles Used To Control Haemonchus Infections : Efficacy In Giraffe (Giraffa Camelopardalis) At Busch Gardens Tampa And Potential Mechanism Of Action, Allyson Kinney Moscona
LSU Master's Theses
Gastrointestinal nematode (GIN) infections affect production systems and exotic hoofstock in zoos, particularly giraffe (Giraffa camelopardalis). Anthelmintic resistance (AR) prevalence is increasing in production systems and zoos. To combat the AR that compounds GIN problems, alternative control methods are used. One such alternative is copper oxide wire particles (COWP), which control the abomasal nematode Haemonchus contortus. COWP was given to seven adult giraffe at Busch Gardens Tampa, at descending dosages: 25 g, 12.5 g, 6.3 g. Treatment administration time was determined by fecal egg count (FEC) being above 600 eggs per gram (EPG), and therefore varied with each animal. FEC …
Evaluation Of Plasma Sprayed Hydroxyapatite Coated Ao Cortical Screws In Equine Third Metacarpal Bone, Myra Elizabeth Durham
Evaluation Of Plasma Sprayed Hydroxyapatite Coated Ao Cortical Screws In Equine Third Metacarpal Bone, Myra Elizabeth Durham
LSU Master's Theses
ABSTRACT Objectives - To compare the osteointegration of plasma sprayed hydroxyapatite (HA) coated and uncoated 5.5 mm cortical screws in equine third metacarpal (MC3) bones in combination with a 4.5mm broad dynamic compression plate (DCP). Study design - In vivo study. Animals – 6 Adult Thoroughbred horses. Methods – For each horse, four HA coated screws were placed unicortically through a 4.5mm broad DCP in the dorsal cortex of a randomly chosen MC3 bone, with 4 uncoated screws placed in an identical manner in the contralateral MC3 bone. All screws were tightened to a torque of 5.4 N-m. Extraction torques …
Isolation And Characterization Of Adult Progenitor Cells From Healthy And Laminitic Hoof Tissue, Vanessa Pinto
Isolation And Characterization Of Adult Progenitor Cells From Healthy And Laminitic Hoof Tissue, Vanessa Pinto
LSU Master's Theses
Laminitis is an often fatal condition in horses with few available and only moderately effective treatment options. Separation of laminar dermis and epidermis lead to rotation or ventral deviation of the third phalanx inside the hoof capsule. Despite being a modified skin, equine laminar tissue does not completely return to normal after laminitis. The hypothesis tested was that adult progenitor cells in the equine laminar tissue are irreversibly damaged by laminitis. A method to harvest and culture cells in vitro from the equine lamina was established; and progenitor cells from unaffected and laminitic hooves were characterized and compared. Laminar tissue …
Role Of Intraocular Leptospira Infections In The Pathogenesis Of Equine Recurrent Uveitis In The Southern United States, Florence Polle
Role Of Intraocular Leptospira Infections In The Pathogenesis Of Equine Recurrent Uveitis In The Southern United States, Florence Polle
LSU Master's Theses
To investigate the role of intraocular leptospiral infections in horses with Equine Recurrent Uveitis (ERU), ocular fluid samples were collected from donated and client-owned horses with a history and ocular findings consistent with chronic ERU. Additionally, eyes were harvested from horses with normal ophthalmic examinations as a control group. Blood samples were obtained for Leptospira serology using microscopic agglutination test (MAT). Aqueous and vitreous humor samples were aseptically obtained and submitted for aerobic culture and Leptospira culture, PCR and MAT. Twenty-one control horses (40 eyes) and 31 ERU horses (46 eyes) were available for study. Serology results were available for …
Immunization Of West Nile Recombinant Envelope Domain Iii With Equine Cd40 Ligand Protein Vaccine Induced Specific Immune Response In Rabbits And Horses, Shiliang Anthony Liu
Immunization Of West Nile Recombinant Envelope Domain Iii With Equine Cd40 Ligand Protein Vaccine Induced Specific Immune Response In Rabbits And Horses, Shiliang Anthony Liu
LSU Master's Theses
West Nile virus (WNV) is one of several flaviruses known to infect mammalian species, including humans. There were 15,257 horse cases reported in 2002 and 1,086 in 2006 in United States. Recently, significant increases in equine and human cases have been reported in United States. Domain III of the WNV envelope protein binds to cellular receptors, and induces a significant portion of the neutralizing antibody response against the virus. CD40 Ligand (CD40L, CD154) enhances productive interactions between T cells and APC and has been shown to function as a potential adjuvant. In this study, we constructed and expressed a fusion …
The Effects Of Iodixanol Present During Equine Semen Cryopreservation, David Paul Beehan
The Effects Of Iodixanol Present During Equine Semen Cryopreservation, David Paul Beehan
LSU Master's Theses
The objectives of this study were to determine what effects iodixanol would have on total and progressive motility, plasma membrane integrity (viability), acrosome integrity and DNA structure when present during cryopreservation of equine spermatozoa,. We hypothesized that the addition of iodixanol would improve post-thaw values for measured parameters. Ejaculates from six stallions were collected, centrifuged at 900 x g for ten minutes to remove supernatant, and suspended to 200 x 106 cells/ml with 0%, 2.5% and 5% iodixanol in an egg-yolk based extender and cryopreserved. Before and after cryopreservation sperm motility was assessed by computer assisted semen analysis, and samples …
Structural And Molecular Pathology Of The Atrium In Boxer Arrhythmogenic Cardiomyopathy, Jorge Luis Vila
Structural And Molecular Pathology Of The Atrium In Boxer Arrhythmogenic Cardiomyopathy, Jorge Luis Vila
LSU Master's Theses
Arrhythmogenic cardiomyopathy (AC), also known as arrhythmogenic right ventricular cardiomyopathy (ARVC), is a disease characterized by fatty or fibro-fatty myocardial replacement, predominantly in the right ventricle and to a lower extent the left ventricle. It is recognized as a disease affecting the cardiac intercalated disc. Clinically, it is associated with ventricular arrhythmias, although atrial arrhythmias and atrial histopathological changes characteristic of AC have occasionally been reported. The full extent of atrial involvement in AC has not been investigated. Therefore, we aimed to apply histopathology, immunochemical detection, immunolocalization and transmission electron microscopy (TEM) techniques to characterize the distribution of desmosomal and …
Biomechanical Evaluation Of Medial And Lateral Approaches For Experimentally Created Medial Condylar Fractures Of The Equine Third Metacarpal Bone, Saybl Beauton Sprinkle
Biomechanical Evaluation Of Medial And Lateral Approaches For Experimentally Created Medial Condylar Fractures Of The Equine Third Metacarpal Bone, Saybl Beauton Sprinkle
LSU Master's Theses
Objective -To compare the compression produced in reduction of experimentally created medial condylar fractures using lag screw fixation with medial vs. lateral approach, and to determine the maximum torque at screw failure. Materials and Methods- Twelve (12) pairs (left and right) 3rd metacarpal bones (MC3) were collected from adult (2-7 years) Thoroughbreds euthanized for reasons unrelated to orthopedic disease. Complete parasagittal medial condylar osteotomies were created at a measurement of 9, 13, and 21 mm axial to the epicondylar fossa on four pairs each of cadaveric MC3 bones resulting in fracture fragments measuring 8, 12, and 20 mm in thickness. …
Mechanical Evaluation Of Aluminum Oxide Roughened Screws In Equine Third Metacarpal Bone, Petrisor Baia
Mechanical Evaluation Of Aluminum Oxide Roughened Screws In Equine Third Metacarpal Bone, Petrisor Baia
LSU Master's Theses
ABSTRACT Objectives – To compare the osseointegration of roughened and electropolished 5.5 mm cortical screws used to secure a 4.5 mm broad dynamic compression plate (DCP) in equine third metacarpal (MC3) bones. Study Design – In vivo study Animals – 5 Adult thoroughbred horses (2-7 years old). Methods – For each horse one MC3 was randomly assigned to secure a 4.5 mm broad DCP with 4aluminum oxide (Al2O3) roughened screws on the dorsal mid diaphysis. Four regular electropolished screws used to secure a similar plate on the contralateral limb served as control. They were removed at 12 weeks and the …
Applicability And Integration Of Plasma Sprayed Hydroxyapatite Coated Ao Cortical Bone Screws In Equine Bone, Timm Gudehus
Applicability And Integration Of Plasma Sprayed Hydroxyapatite Coated Ao Cortical Bone Screws In Equine Bone, Timm Gudehus
LSU Master's Theses
To compare insertion temperatures and torques of Hydroxyapatite (HA) coated and uncoated 5.5 mm AO cortical screws in equine third metatarsal bones (MTIII) in vitro, and to compare insertion and extraction torques of HA coated and uncoated screws after 4, 8, 12 and 16 weeks of healing in equine third metacarpal bones (MCIII) in vivo. No significant temperature differences were recorded in cadaveric bones for AO and HA coated screws. Insertion torques were significantly higher for HA coated implants compared to uncoated screws. In vivo, the AO screws lost 50% of their initial stability within 4 weeks of healing and …
Carprofen-Induced Oxidative Stress In Mitochondria Of The Colonic Mucosa Of The Dog, Lynne A. Snow
Carprofen-Induced Oxidative Stress In Mitochondria Of The Colonic Mucosa Of The Dog, Lynne A. Snow
LSU Master's Theses
Objectives 1) To measure conductance and permeability of canine colonic mucosa exposed to increasing concentrations of carprofen. 2) To compare conductance and permeability of canine colonic mucosa exposed to carprofen or 2,4-dinitrophenol (DNP) and tempol blockade. Design In vitro randomized block design Animal 20 mixed breed dogs Methods Conductance, mannitol flux, and histology were evaluated in colonic mucosa mounted in Ussing chambers. Mucosa was first exposed to increasing concentrations of carprofen. Mucosa was then exposed to either carprofen (200 μg/ml) or DNP (0.25mM) +/- tempol (1mM) pretreatment. Conductance over time, mannitol fluxes, and frequency of histologic categories were analyzed for …
Effects Of Clenbuterol On Skeletal And Cardiac Muscle In Horses, Jessica Thompson
Effects Of Clenbuterol On Skeletal And Cardiac Muscle In Horses, Jessica Thompson
LSU Master's Theses
Clenbuterol is a commonly prescribed β2-adrenergic agonist approved for veterinary use as a bronchodilator in horses with reactive and obstructive airway disease. Potential for abuse of this drug in the horse industry is substantial, due to the perceptions that clenbuterol increases performance and lean muscle mass. Although anabolic effects have been confirmed in multiple species, recent studies into the effects of clenbuterol in exercising horses suggest that clenbuterol doses within therapeutic ranges negatively impact aerobic capacity and cardiac function. Results of studies in murine models demonstrate that clenbuterol directly induces skeletal and cardiac muscle cell death at high doses. Three …
Chondrocyte Death In Canine Osteochondral Explants Exposed To 0.5 Percent Bupivacaine, Geoffrey Stuart Hennig
Chondrocyte Death In Canine Osteochondral Explants Exposed To 0.5 Percent Bupivacaine, Geoffrey Stuart Hennig
LSU Master's Theses
Objective: Our in vitro study evaluated chondrocyte death in canine articular cartilage exposed to 0.5% bupivacaine without (B) and with methylparaben (BP) and compared the viability with intact (SI) and mechanically debrided surfaces (SR). We hypothesized that B or BP would cause chondrocyte death in vitro in a time dependent manner and that chondrocyte death would be increased in SI osteochondral cores compared to SR. Materials and Methods: Intact cartilage cores with underlying subchondral bone were collected aseptically from both humeral heads of 16 fresh canine cadavers. Joints from each dog were randomly assigned to SI or SR. Ten osteochondral …
Influence Of Tick Transmission On The Host Response To Rickettsial Infection, Soma Chowdhury
Influence Of Tick Transmission On The Host Response To Rickettsial Infection, Soma Chowdhury
LSU Master's Theses
Several members of the spotted fever group (SFG) of Rickettsia are transmitted to the host through infected ixodid (hard) ticks, which can serve as both vectors and reservoirs. Multiple studies have demonstrated that ticks secrete proteins into the bite site of the host that suppress innate and adaptive immune responses. While this suppression of immune responses is beneficial to the tick, it may also be beneficial to the transmitted Rickettsia. We hypothesize that Rickettsia utilize the tick’s ability to alter the host immune response at the tick feeding site to successfully establish infection. In the current study, we analyzed how …
Quantitative Real-Time Polymerase Chain Reaction (Qpcr) Assay As A Molecular Tool To Assess Rickettsial Replications In Tick Hosts, Andrea S. Zanetti
Quantitative Real-Time Polymerase Chain Reaction (Qpcr) Assay As A Molecular Tool To Assess Rickettsial Replications In Tick Hosts, Andrea S. Zanetti
LSU Master's Theses
During the past century, many species of the Spotted Fever Group Rickettsia (SFGR)have been described, especially, through the introduction of a variety of molecular techniques applied to detect rickettsiae inside of their host. In this study we developed a quantitative realtime polymerase chain reaction (qPCR) assay (1) to characterize the growth and the distribution of a SFGR of unrecognized pathogenicity in naturally infected Amblyomma americanum ticks during physiological events; and (2) to terminate the influence of the host cell specificity in the replication patterns of recognized and unrecognized SFGR during a reciprocal rickettsiae challenge in both mammalian and tick cell …
Biomechanical Evaluation Of A 6.5-Mm Headless, Tapered Variable Pitch Screw (Acutrak Plus) In Equine And Synthetic Bone, Andrew James Czak Lewis
Biomechanical Evaluation Of A 6.5-Mm Headless, Tapered Variable Pitch Screw (Acutrak Plus) In Equine And Synthetic Bone, Andrew James Czak Lewis
LSU Master's Theses
Objectives – To compare compression pressure (CP) of 6.5mm Acutrak Plus (AP) and 4.5mm AO cortical screws (AO) when placed in simulated lateral condylar fractures of variable thickness in cadaveric equine third metacarpal (MC3) bones, and to compare pullout force and strength of AP and AO screws when placed in a synthetic bone substitute. Results – The AO screw configurations generated significantly greater compressive pressure compared to the AP configurations. The ratio of mean CP for AP screws to AO screws at 20, 12, and 8-mm, were 21.6%, 26.2%, and 34.2% respectively. For the pullout study, the AP constructs generated …
Effects Of Centrifugation On Equine Spermatozoa Immediately And After Cooling For 24 Hours, Jose Augusto Len
Effects Of Centrifugation On Equine Spermatozoa Immediately And After Cooling For 24 Hours, Jose Augusto Len
LSU Master's Theses
Len, Jose Augusto, MVZ, Universidad de Guadalajara, 1994 Master of Science, Summer Commencement, 2008 Major: Veterinary Medical Sciences Effects of Centrifugation on Equine Spermatozoa Immediately and After Cooling for 24 Hours Thesis directed by Professor Bruce Eilts Pages in thesis, 60. Words in abstract, 245. ABSTRACT The objectives of this study were to determine the effects of centrifugation on equine sperm progressive motility, plasma membrane integrity (viability), and acrosome integrity. We hypothesized that high centrifugation forces would be detrimental to equine sperm, yet recovery rates would increase. Ejaculates from six stallions were collected, extended (INRA96) to a concentration of 25 …
Comparative Roles Of Herpes Simplex Virus Type 1 (Hsv-1) Glycoproteins In Cytoplasmic Virion Egress, Hyuncheol Lee
Comparative Roles Of Herpes Simplex Virus Type 1 (Hsv-1) Glycoproteins In Cytoplasmic Virion Egress, Hyuncheol Lee
LSU Master's Theses
HSV-1 acquires its final envelope by budding into cytoplasmic vesicles thought to be derived from Trans-Golgi Network (TGN) membranes. This process is facilitated by interactions among the carboxyl termini of viral glycoproteins and tegument proteins. To investigate the relative importance of different viral glycoproteins in cytoplasmic virion morphogenesis, a set of recombinant viruses were constructed silencing expression of the glycoprotein E (ΔgE), the carboxyl terminus of glycoprotein D (gDΔcp), and the membrane protein UL20 (ΔUL20). In addition, recombinant viruses were constructed having the ΔgE+gDΔcp, and the ΔgE+ΔgM (glycoprotein M) deletions. These recombinant viruses were constructed using the double-red, site-directed mutagenesis …