Veterinary Medicine Commons^™

Development And Diagnostic Application Of Monoclonal Antibodies Against Swine Acute Diarrhea Syndrome Coronavirus (Sads-Cov), Molly Kroeger

Schultz-Werth Award Papers

Swine Acute Diarrhea Syndrome Coronavirus (SADS-CoV) is a member of the Coronaviridae family. The virus is associated with severe small intestine inflammation and diarrhea in suckling piglets. In 2017, SADS-CoV was first detected and identified as the causative agent of a devasting swine disease outbreak in southern China. Routine monitoring and early detection of the source of infection is therefore integral to the prevention and control of a SADS-CoV outbreak in the United States. However, the United States does not currently have any diagnostic or surveillance tests to identify this emerging disease. To address these industry needs, we developed monoclonal …

Growing South Dakota (Winter 2020), College Of Agriculture, Food And Environmental Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

This issue contains the SDSU Extension 2019 Annual Report

[Page] 2-3 South Dakota Animal Disease Research and Diagnostic Laboratory Ribbon Cutting Ceremony
[Page] 4 SDSU Extension
[Page] 4 From the Director of Extension Karla Trautman
[Page] 5 2019 USDA Risk Management Agency Crop Indemnities
[Page] 6-8 SDSU Extension Responds to Mother Nature in 2019
[Page] 9 Successful Energize! Conference Buoys Community Efforts in Lemmon & Beyond
[Page] 10-11 Helping Loved Ones Transition Smoothly
[Page] 12 AmeriCorps VISTA Members Expand Extension Programming
[Page] 13 South Dakota Wellness Coalitions Make Big Impacts in Small Communities [Page] 14-16 Extension Programs in Our Own …

Growing South Dakota (Summer 2019), College Of Agriculture, Food And Environmental Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 3 Celebration of Faculty Excellence
[Page] 6 Ag and Biosystems Engineering - Quarter Scale Tractor Team Named Reserve National Champs - Jackrabbit Wrestler Passionate About Sharing the Story of Pork - Mesonet Spray Tool Aids Precision Application Decisions
[Page] 10 Ag Business and Economics -POET Commodity Trading Fund
[Page] 12 Ag Education, Communication and Leadership - Leadership Opportunities Launch Recent Graduate to Career Readiness
[Page] 14 Agronomy, Horticulture & Plant Science - Local Foods Education Center Donates Over 5,000 Pumpkins - Seeding Success - Doug Malo Retires After 43 Years of Teaching - Howard Woodard's 29-Year Career Had a …

Senecavirus A: Pathogenicity And Interactions With Host Cell Death Pathway, Maureen Hoch Vieira Fernandes

Electronic Theses and Dissertations

Senecavirus A (SVA) is a positive sense single-strand RNA virus of the genus Senecavirus, family Picornaviridae. Senecavirus A is an emerging picornavirus of swine, which causes vesicular disease (VD) in pigs that is clinically indistinguishable from footand- mouth disease and other high consequence VDs of swine. There is evidence that SVA has been circulating in the US swine population since the late eighty’s, however, recently the virus emerged worldwide, and it has been associated with several VD outbreaks in pigs in many of the major swine producing countries. Despite the recent surge in the number of cases of …

Alternative Treatment Options For Controlling Anthelmintic-Resistant Haemonchus Contortus Populations In South Dakota Sheep Herds, Adam Sarah

Electronic Theses and Dissertations

Haemonchus contortus is a common economically important gastrointestinal nematode that obliges the survival and productivity of small ruminants, especially sheep and goats. Anthelmintic resistance is a primary challenge in ruminant health management programs in preventing and controlling the H. contortus populations; therefore, there is a great necessity in finding and developing natural plant products for use as alternatives to commercial anthelmintics for controlling H. contortus in pastured sheep and goats. This dissertation has three major aims conducted in three separate studies: the first in vivo one was to evaluate the extended effect of a springtime, combined-treatment with 3 anthelmintics having …

Rumen Microbial Culture Library And In Vitro Analysis Of Selected Bacterial Species On Colonization Resistance Against Bovine Enteric Pathogens, Maria Antony

Electronic Theses and Dissertations

The anaerobic habitat of the four chambers in ruminant’s foregut plays a vital role in the fermentation of the food by the active involvement of the residing microbial community. The cultivability and the potential role of rumen bacteria in the field of livestock production, host health and immunity are important to study. The developed anaerobic culturing techniques enable us to culture and screen different rumen bacteria to study the anti-pathogenic effects. Whole genome sequencing and analysis help us to understand the genome level characteristics of the rumen bacterial species individually. Since the majority of the rumen biosphere is still not …

Performance Differences Between Two Groups Of Commercial Pigs Following Experimental Infection With Prrs Virus 1-7-4, Erin Little

Electronic Theses and Dissertations

Porcine reproductive and respiratory syndrome virus (PRRSV) is the costliest swine disease in North America. Vaccines and management alone have not been effective in controlling this disease. Genetic selection for resilience may be a complimentary approach for controlling PRRSV. The objective of this study was to estimate performance differences between two groups of pigs from the same commercial line following infection with PRRSV 1-7-4: 1) pigs sired by boars selected based on a standard index (TN-S), which emphasized feed efficiency and carcass quality; and 2) pigs sired by boars selected based on an experimental index (TN-E), which emphasized feed intake, …

Development And Evaluation Of Vaccine Candidates For Senecavirus A, Bishwas Sharma

Electronic Theses and Dissertations

Senecavirus A (SVA) has been associated with several outbreaks of vesicular disease (VD) since 2014 in major swine-producing countries around the world. The virus causes VD that is clinically indistinguishable from foot-and-mouth disease (FMD) in pigs. Currently there are no commercial vaccines available for SVA. Here, we developed a live attenuated SVA strain by mutating a wild type SVA strain SD15-26 (wt SVA). The live attenuated virus, rSVA mSacII, replicated less efficiently in cell cultures in vitro and expressed lower amounts of VP1 and VP2 capsid proteins than wt SVA. Most importantly, the rSVA mSacII virus failed to induce clinical …

Bovine Cellular And Innate Immune Response To Hemostatic Polysaccharides, Brian Kobylkevich

Electronic Theses and Dissertations

Chronic wounds are longstanding dermal wounds that do not heal in a normal amount of time and can commonly last for over a year. These wounds plague humans and farm animals alike. Wounds are characterized by chronic inflammation and are often painful, difficult to heal, have a high recurrence rate, and are potentially debilitating. Long term wounds that do not fully heal are distressing for both humans and animals, but understandably more resources have been directed towards humans. Some treatment options for humans may be suitable for adaptation into the field of animal care. Chronic lesions such as bovine digital …

Growing South Dakota (Winter 2019), College Of Agriculture, Food And Environmental Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

This is issue contains the South Dakota Agricultural Experiment Station 2018 Annual Report, and the SDSU Extension 2018 Annual Report.

[Page] 2 Setting Into role as Dean
[Page] 4 Raven Precision Ag Center Groundbreaking Ceremony
[Page] 10 Collaborating for Research-Based Solutions
[Page] 12 From the SDSU Extension Director
[Page] 14 Empowering South Dakotans Through Training and Certifications
[Page] 18 Mental Health and Opioid Education
[Page] 21 Science of Agriculture
[Page] 23 Community Gardens Bring Hope to Pine Ridge Indian Reservation
[Page] 26 Energize! Conference
[Page} 28 Disaster Preparedness Training
[Page] 30 Bush Foundation Change Network
[Page] 32 SDSU to Participate …

Growing South Dakota (Winter 2018), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 Learn About Our Leaders
[Page] 4 Academic Programs at a Glace
[Page] 6 New Precision Ag Major Takes Off
[Page] 9 Raven Gives $5 Million Gift
[Page] 10 New Plant Science Research Facility
[Page] 13 Technical Training to Four-Year Degree
[Page] 15 Ag-Bio Career Fair Hits Record
[Page] 16 Breaking Ground : SD ADRDL
[Page] 19 Distinguished Professor Eric Nelson
[Page] 20 SDSU’s Veterinary Students
[Page] 24 Conservation Planning and Park Management
[Page] 25 Natural Resource Law Enforcement
[Page] 26 Medical Stethoscope Ceremony
[Page] 29 Master’s Degree in Human Biology
[Page] 30 Scott Pedersen Prepares Students
[Page] 32 …

Evaluation Of Berberine As An Alternative To Antibiotics In Nursery Pig Diets, Emily Scholtz

Electronic Theses and Dissertations

Pig weaning process results in reduced growth performance and gut health of weaned pigs. Antibiotics can be added in diets for weaned pigs to improve growth performance and gut health, but their use is being discouraged because they can lead to development of antimicrobial resistant microorganisms. Thus, there is need for alternatives to antibiotics in diets for weaned pigs. The overall goal of this thesis research was to determine the effects of plant extracts (berberine, quercetin, and allyl isothiocyante [AITC]) as alternatives to antibiotics in weaned pig diets. The first objective of the research was to determine in vitro antimicrobial …

Universal Swine Influenza Antigen And Vaccine, Zhao Wang

Electronic Theses and Dissertations

Swine influenza A viruses pose a serious concern for global health and worldwide swine industry considering their evasion from vaccine-induced immune responses. Formulation of effective influenza vaccines can be complicated by both antigenic shift to a different subtype of hemagglutinin (HAs) and antigenic drift within a particular HA subtype. To address these concerns, we employed a systematic approach to determine the antigenic determinants of swine influenza A viruses by focusing on H1 and H3 subtypes. In parallel, we used a cross-disciplinary approach including structural biology and modeling, virology, and immunology to identify swine influenza A virus-derived HA fusion intermediates broadly …

Serological Evidence For The Co-Circulation Of Two Lineages Of Influenza D Virus In Equine Populations Of The Midwest United States, Hunter Theodore Nedland

Electronic Theses and Dissertations

Influenza D virus (IDV) is a newly described lineage of the Orthomyxoviridae virus family that was first isolated from diseased swine in 2011 and has subsequently been detected in cattle around the world in 2014. In addition, serological evidence for IDV infection in humans has been recently established. Despite all the progress, the full range of susceptible hosts for this novel virus has yet to be determined, but includes swine, bovine, small ruminants and human. This study was designed to determine if equine is a possible host to this newly emerging influenza virus. 364 equine serum samples were collected in …

The Effects Of Bovine Viral Diarrhea Virus And Bovine Herpesvirus Type 1 On Monocyte-Derived Dendritic Cells, Jacob Sobraske

Electronic Theses and Dissertations

Bovine viral diarrhea virus (BVDV) and bovine herpesvirus type 1 (BHV-1) are important pathogens that affect cattle. In this in vitro study, the main goal was determining the direct effects of BVDV and BHV-1 infections of monocyte-derived dendritic cells (MDDC) on cytokine mRNA expression. For the BVDV infected MDDC experiments, Holstein Friesian and Brown Swiss calves were included with a breed comparison with cytokine expression. Surface marker expression was also measured in BHV-1-infected MDDCs. For both viruses, a high virulent strain (1373 for BVDV and Cooper for BHV-1) and lower virulent strains (20508 for BVDV and Los Angeles for BHV-1) …

Effects Of Enzymatically Hydrolyzed Yeast Supplementation And Supplementation Frequency On Immune Parameters, Performance, And Digestibility Among Periparturient Beef Cows And Calves, Janine Swartz

Electronic Theses and Dissertations

Effects of enzymatically hydrolyzed yeast (EHY) and supplementation frequency (FREQ) on immune parameters among beef cows and calves was evaluated. Eighty multiparous (parity = 4.2 ± 0.3) cows were fed a common brome hay-based diet (CP = 8.0 ± 0.17%). Cows were blocked by expected calving date and stratified by body condition score (BCS) before random assignment of treatment. Beginning 88 ± 5 d prior to parturition, cows were provided 1 kg daily or 3 kg every 3 d of a soybean hull-based supplement (CP = 34.0, % of DM) that contained 0 or 3 g/kg EHY. The daily supplement …

Growing South Dakota (Summer 2017), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

This issue contains the 2016 SDSU Extension Annual Report.

Page] 2 From the Director
[Page] 3 WaterTouches Everything, Multi-State Network Strives to Enhance Water Stewardship
[Page] 6 From RubbleTo Renewal, Unique Art Project Helps Delmont Community Heal
[Page] 7 Future Focused, Community Development Program Helps Harness Ideas Into Action
[Page] 8 Health Help, Statewide Program Strives To Help Individuals Better Manage Chronic Health Condition
[Page] 10 Making An Impact, Master Gardener Program Helps Individuals & Communities Flourish
[Page] 12 Statewide Highlights & Happenings
[Page] 14 Outreach to Youth, Programs Foster Engagement With Diverse Youth Audiences
[Page] 17 A Look At …

Growing South Dakota (Spring 2017), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

This issue contains the 2016 South Dakota Agricultural Experiment Station Annual Report.

[Page] 3 Opportunities With Oats SDSU Researchers Strive to Improve Varieties [Page] 6 Stopping Stem Canker Plant Pathologists Making Progress To Keep This Disease At Bay
[Page] 7 Disease Detectives Vet Scientists Researching Another Emerging Virus [Page] 8 Community Support Devastating West River Fire Brings Ag Industry Together
[Page] 10 Research In Progress Dakota Lakes Research Farm Investigates Three Big Ideas
[Page] 14 Water Quality Quest Understanding E. Coli Behavior Aims To Aid Water Quality In Rivers & Streams
[Page] 15 Verifying Ag Land Valuation Researchers Assist In …

The Indirect Effect Of Bvdv On Immune Suppression: The Role Of Infected Macrophages In Lymphocyte Apoptosis, Karim Abdelsalam

Electronic Theses and Dissertations

BVDV is an important pathogen of cattle that affects both the dairy and beef industry causing severe economic losses. The main problem of BVDV is the immune suppression that is associated with lymphoid depletion shortly post infection. The mechanism of this immune suppression is not well understood. In this in vitro study, we sought to determine the indirect effect of BVDV infection in macrophages as a key player in the pathogenesis of BVD especially, the apoptotic effect induced on lymphocytes. We infected bovine monocyte-derived macrophages (MDM) with either high or low virulent strains of cytopathogenic or non-cytopathogenic strains of BVDV …

Intranasal Immunization Of Pigs With Porcine Reproductive And Respiratory Syndrome Virus Like Particles Plus 2’, 3’ –Cgamp Vaccigrade™ Adjuvant Exacerbates Viremia After Virus Challenge, Alexandria Marie Van Noort

Electronic Theses and Dissertations

Porcine reproductive and respiratory syndrome virus (PRRSV) is an economically devastating virus that causes a persistent infection and spreads quickly. The virus causes abortions in sows and respiratory diseases in young piglets. Current PRRSV vaccines appear to provide limited cross-protection. A virus-like particles (VLPs) vaccine is made up of key PRRSV structural proteins, which could be cross protective among the different strains. We tested PRRSV VLPs as a vaccine candidate with a 2’, 3’-cGAMP VacciGradeTM adjuvant against pigs infected with PRRSV after intranasal immunization to evaluate the immune response and protective efficacy by the VLPs and adjuvant. The VLPs contain …

Reagents And Tools To Identify Epizootic Hemorrhagic Disease Virus-2, Meghan Syrstad

Electronic Theses and Dissertations

The purpose of this project was to define monoclonal antibodies against viral proteins from Epizootic Hemorrhagic Disease Virus (EHDV) to be used in quality control of vaccines produced by an industrial partner, and development of tools to identify EHDV. EHDV is an Orbivirus, which also includes Bluetongue Virus and African Horse Sickness, which are all transmitted through arthropod vectors. EHDV causes significant morbidity and mortality in white-tailed deer, but has recently been found to infect cattle. EHDV recently caused significant outbreaks affecting both the farmed and wild cervid industry, however few reagents and tools exist to protect against this disease …

Senecavirus A: Epidemiology, Pathogenesis And Infection Dynamics, Lok Raj Joshi

Electronic Theses and Dissertations

Senecavirus A (SVA) is a positive sense single-stranded virus that belongs to the genus Senecavirus within the family Picornaviridae. Senecavirus has been present in US swine population since 1988. However, there have been only a few reports of SVA associated with disease in last two decades. In late 2014 early 2015, a sudden increase in the number of cases of Senecavirus A associated with vesicular disease was observed in Brazil, the US and China. Affected pigs presented vesicles on snout, coronary band, and dewclaw. Additionaly, increased neonatal mortality has been reported in the field. Many aspects of SVA epidemiology, …

Surveillance Of Emerging Livestock Viruses, Faten A. Okda

Electronic Theses and Dissertations

Accurate and rapid diagnostic tests are essential for the management and control of emerging infectious diseases. Therefore, the overall goals of studies presented in this dissertation were to provide improved diagnostic and surveillance capabilities for several emerging viral diseases impacting the livestock industry and to provide insight into appropriate control strategies. Recent, severe outbreaks of porcine epidemic diarrhea virus (PEDV) in Asia and North America highlighted the need for well-validated diagnostic tests for identification of PEDV infected animals and evaluation of their immune status. PEDV was first detected in the U.S. in May 2013 and another swine coronavirus, porcine deltacoronavirus …

Understanding The Effect Of Bvdv On Innate Immune Response Of Neutrophils, Neelu Singh Thakur

Electronic Theses and Dissertations

Bovine viral diarrhea virus (BVDV) is one of the highly prevalent and economically important diseases of cattle industry worldwide. The two major consequences of this disease are persistent infection and immunosuppression. Several studies have been done to determine the underline mechanisms of BVDV-induced immunosuppression targeting antigen presenting cells, adaptive immune system cells and cytokine gene expression. However, very little research has been done to determine the effect of BVDV on neutrophils. Neutrophils are one of the most abundant while blood cells (WBC) in the peripheral blood, which play a critical role in the innate as well as adaptive immune response. …

Animal Health Matters, Jane Hennings, Larry Holler, Russ Daly, Linda Fawcett

Animal Health MATTERS Newsletter

[Page] 1 SDSU and SHIC Study on Senecavirus Offers Keen Insights
[Page] 2 Director's Message: Innovation in the Laboratory and Being Proactive in the Protection of Animal Health
[Page] 2 Bovine Abortion-Sample Submission Guidelines
[Page] 3 South Dakota One Health Meeting Covers Bacterial Zoonotic Disease
[Page] 3 Small Ruminant, Poultry Serology Offerings Expanded at the ADRDL
[Page] 4 Molecular Diagnostics Section Evolves, Adds New Tests
[Page] 5 Recent PCR tests Added by ADRDL
[Page] 6 New Employees
[Page] 7 ADRDL Continues Their Critical Role Supporting Animal Exports

Growing South Dakota (Summer 2016), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 Breaking New Ground SDSU Leads Nation With Precision Agriculture Initiative
[Page] 6 In The Driver's Seat Utility Vehicles Provide Students Hands-On Precision Ag Experience
[Page] 7 Introduction To Industry Travel To National Events Opens Opportunities For Students
[Page] 9 On The Ground SDSU Extension Specialists Have Vital Role In Bringing Data To Producers
[Page] 10 Curricula Collaboration SDSU Leaders Among Team Preparing Future Precision Ag Coursework
[Page] 12 Future Focus Ag 2020 Plan Prompts Important Reinvestments In State's Ag Experiment Station
[Page] 13 The Power Of Connections Connected Approach Helps Solve "Real-Life" Problems To Benefit Animal And …

Growing South Dakota (Winter 2016), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

This issue includes the 2015 SDSU Extension Annual Report.

[Page] 2 Contours Program Launched: Aims to Provide Insights & Prompt Discussions
[Page] 4 Local Momentum: Formation of State's First “Food Hub” Comes To Fruition [Page] 6 Spotlight On Soil Health: New Coalition Formed
[Page] 8 Exploring A Range Of Options: Youth Programs Cultivate Interest In Natural Resource Careers
[Page] 9 Profiles In Leadership: Our Mission Continues… Ensuring All Citizens Have Access To Education & Emerging Innovation
[Page] 10 Encouraging Animal Well-Being Workshops & Trainings Help Livestock Producers Become Better Stewards
[Page] 12 Wellness Wisdom: Variety Of Efforts Available To Encourage …

Animal Health Matters, Russ Daly, Jane Hennings

Animal Health MATTERS Newsletter

[Page] 1 VBSD/ADRDL Researchers Benefit from new Oscar Olson Research Laboratories at SDSU
[Page] 2 Director's Message: The ADRDL in State Statute
[Page] 3 Darrell Johnson Honored by Department
[Page] 3 Pork Quality Assurance Advisor Training Scheduled at SDSU
[Page] 4 Specimen Receiving and Necropsy Sections at the ADRDL: Getting the Diagnostic Process off to a Great Start
[Page] 6 New Anaplasmosis PCR test online at ADRDL
[Page] 6 Laura Schanning Joins ADRDL in Specimen Receiving
[Page] 6 Reminder: Case History Helps Calf Scours Diagnosis
[Page] 6 Dr. Beverly Shelbourn Joins Veterinary and Biomedical Sciences Department as Instructor
[Page] 6 …

Growing South Dakota (Winter 2016), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

This issue includes the 2015 South Dakota Agricultural Experiment Station Annual Report.

[Page] 2 Director's Message
[Page] 3 Concentrating On Cattle: Researcher Studying Mechanics To Improve Reproduction Efficiency In Beef Cattle
[Page] 5 At The Forefront: SDSU Diagnostic Lab Scientists Sequence Avian Influenza Genome
[Page] 6 Seeking Superior Wheat: Breeder Using Extensive Genome Testing To Improve Winter Wheat Cultivars
[Page] 8 Profiles In Leadership: Plant And Fungi Interactions May Offer New Plant Production Possibilities
[Page] 9 Targeting Emerging Diseases: Plant Pathologists Working To Better Manage Soybean & Sunflower Diseases
[Page] 10 Compelling New Crop: Carinata Offers Potential For Crop Diversity, …

Development And Characterization Of A Recombinant Orf Virus Vector Expressing The Spike Protein Of Porcine Epidemic Diarrhea Virus, Kyle Hain

Electronic Theses and Dissertations

Orf virus (ORFV), the type member of the genus Parapoxvirus of the family Poxviridae, causes orf or cutaneous pustular dermatitis in sheep and goats. ORFV is a ubiquitous virus capable of re-infecting its hosts multiple times over time. ORFV causes a non-systemic, self-limiting disease which is usually restricted to the skin surrounding the virus entry sites. ORFV has evolved several immunomodulatory proteins (IMPs) that evade and/or modulate host immune responses to infection and contribute to virus virulence and disease pathogenesis. Given biological properties and unique immunomodula tor y properties, ORFV has gained significant attention in recent years for its …