Veterinary Medicine Commons^™

Evaluating The Prevalence And Effectiveness Of Breed-Specific Legislation, Felicia E. Trembath

Open Access Dissertations

Dog bites pose a persistent public health problem, which some jurisdictions pass breed-specific legislation (BSL) to address. However, very little non-anecdotal evidence regarding the efficacy of BSL has been presented. Currently, BSL research is hampered by the absence of standard terminology, an established prevalence, or a scientific consensus on its effectiveness. The purpose of this study is to propose standardized terminology for BSL, establish the prevalence of each type of BSL in the USA, and conduct a systematic review of the effectiveness of BSL.

After review of terminology currently in use, as well as review of the regulatory actions of …

Evaluation Of Negative Energy And Mineral Balance In Periparturient Holstein-Friesian Cattle, Ameer Ahmed Abdelhameed

Open Access Dissertations

Most cows experience a state of negative energy and mineral balance during the periparturient period. This imbalance is a major problem in the dairy industry and it is more frequently seen in multiparous cattle due to their high milk production. A large amount of fat and protein is mobilized in order to address the energy deficit during the periparturient period. The ability to accurately monitor energetics and electrolytes status using accurate, rapid, and low cost point-of-care instruments will assist in optimizing animal health and productivity.

This dissertation contains three studies that evaluated point-of-care instruments for measuring blood and plasma glucose, …

The Effects Of Acidified Pepsin On Porcine Vocal Fold Tissue: Developing A Porcine Model Of Laryngopharyngeal Reflux Disease, Abigail Cox Durkes

Open Access Dissertations

Approximately 7.5 million Americans are affected by a voice disorder. In the last year, 7.2% of people missed one or more days of work due to a voice problem. For professional voice users, such as teachers, the rate increases to 20% and an annual cost of $2.5 billion. Voice disorders are complex and multi-faceted, as well as difficult to diagnose and treat. Prospective animal studies are necessary to study the pathophysiology of voice disorders, optimize our understanding of laryngeal pathology, and improve treatment outcomes. The pig provides a unique opportunity to test hypotheses relating to laryngeal disease because porcine vocal …

Repurposing Celecoxib As A Topical Antimicrobial Agent, Shankar Thangamani, Waleed Younis, Mohamed N. Seleem

Department of Comparative Pathobiology Faculty Publications

There is an urgent need for new antibiotics and alternative strategies to combat multidrug-resistant bacterial pathogens, which are a growing clinical issue. Repurposing existing approved drugs with known pharmacology and toxicology is an alternative strategy to accelerate antimicrobial research and development. In this study, we show that celecoxib, a marketed inhibitor of cyclooxygenase-2, exhibits broad-spectrum antimicrobial activity against Gram-positive pathogens from a variety of genera, including Staphylococcus, Streptococcus, Listeria, Bacillus, and Mycobacterium, but not against Gram-negative pathogens. However, celecoxib is active against all of the Gram-negative bacteria tested, including strains of, Acinetobacter, and Pseudomonas, when …

Baylisascaris Spp. In Non-Raccoon Procyonid Hosts And Assessment Of Potential Risk Of Human Exposure, Max Carlin Parkanzky

Open Access Theses

Baylisascaris procyonis (Bp) is a large roundworm of the common raccoon (Procyon lotor) which serves as the definitive host. Bp is an important cause of clinical larva migrans, including severe neurological disease, across numerous taxa including humans. Other procyonids, as well as occasionally dogs, can act as definitive hosts for this or other Baylisascaris spp. Many of these animals are becoming more common as household pets, posing a risk to people who come in contact with these animals. We have investigated whether patent Baylisascaris spp. infection exists in captive non-raccoon procyonids and if humans who contact these animals …

Show Horse Welfare, Melissa A. Voigt

Open Access Dissertations

In recent years there has been an increase in the public's attention to situations where trainers, owners, and handlers have compromised the well-being of show horses for the sake of winning. These situations may be due to training negligence or naivety of individuals working with the horse. Either way, due to these incidents, increasing pressure has been placed on the horse industry to address show horse welfare. The purpose of this research was to expound on the welfare of stock-type show horses through the perspective of those directly involved; considering the understanding of welfare, the value placed on welfare, and …

Antibacterial Activity Of Novel Cationic Peptides Against Clinical Isolates Of Multi-Drug Resistant Staphylococcus Pseudintermedius From Infected Dogs, Mohamed F. Mohamed, G. Kenitra Hammac, Lynn F. Guptil, Mohamed N. Seleem

Department of Comparative Pathobiology Faculty Publications

Staphylococcus pseudintermedius is a major cause of skin and soft tissue infections in companion animals and has zoonotic potential. Additionally, methicillin-resistant S. pseudintermedius (MRSP) has emerged with resistance to virtually all classes of antimicrobials. Thus, novel treatment options with new modes of action are required. Here, we investigated the antimicrobial activity of six synthetic short peptides against clinical isolates of methicillin-susceptible and MRSP isolated from infected dogs. All six peptides demonstrated potent antistaphylococcal activity regardless of existing resistance phenotype. The most effective peptides were RRIKA (with modified C terminus to increase amphipathicity and hydrophobicity) and WR-12 (α-helical peptide consisting exclusively …

Ontogenetic Scaling Patterns And Functional Anatomy Of The Pelvic Limb Musculature In Emus (Dromaius Novaehollandiae), Luis P. Lamas, Russell P. Main, John R. Hutchinson

Department of Basic Medical Sciences Faculty Publications

Emus (Dromaius novaehollandiae) are exclusively terrestrial, bipedal and cursorial ratites with some similar biomechanical characteristics to humans. Their growth rates are impressive, as their body mass increases eighty-fold from hatching to adulthood whilst maintaining the same mode of locomotion throughout life. These ontogenetic characteristics stimulate biomechanical questions about the strategies that allow emus to cope with their rapid growth and locomotion, which can be partly addressed via scaling (allometric) analysis of morphology. In this study we have collected pelvic limb anatomical data (muscle architecture, tendon length, tendon mass and bone lengths) and calculated muscle physiological cross sectional area …

Efficacy Of Short Novel Antimicrobial And Anti-Inflammatory Peptides In A Mouse Model Of Methicillin-Resistant Staphylococcus Aureus (Mrsa) Skin Infection, Mohamed F. Mohamed, Mohamed N. Seleem

Department of Comparative Pathobiology Faculty Publications

The therapeutic efficacy of two novel short antimicrobial and anti-inflammatory peptides (RR and RRIKA) was evaluated in a mouse model of staphylococcal skin infection. RR (2%) and RRIKA (2%) significantly reduced the bacterial counts and the levels of proinflammatory cytokines, tumor necrosis factor (TNF)-α, and interleukin (IL)-6, in methicillin-resistant Staphylococcus aureusUSA 300-0114 skin lesions. Furthermore, the combined therapy of RRIKA (1%) and lysostaphin (0.5%) had significantly higher antistaphylococcal and anti-inflammatory activity compared to monotherapy. This study supports the potential use of these peptides for topical treatment of methicillin-resistant Staphylococcus aureus skin infections.

Review Of The Pathogenesis And Treatment Of Acute Spinal Cord Injury And Investigation Into The Use Of Urine 3-Hpma As A Novel Biomarker Of Secondary Injury After Acute Spinal Cord Injury In The Dog, Andrea Sangster

Open Access Theses

Acute spinal cord injury (ASCI) has two pathophysiological stages of injury: the primary injury and the secondary injury cascade. Primary injury includes the initial or mechanical insult to the spinal cord. Secondary injury is a cascade of biochemical events that propagates damage of adjacent, healthy tissue increasing the overall volume of spinal cord tissue that is affected. Acrolein is a toxic byproduct of lipid peroxidation produced during secondary injury. A metabolite of acrolein-glutathione adduct found in urine, 3-HPMA, has recently been shown to increase after spinal cord injury in a rat model. The aim of our study was to apply …

The Effects Of Heat Stress On Immunity In Laying Hens And Dairy Cattle, Rebecca Ann Strong

Open Access Theses

With the increase in global climate change and the population growth driving the high demand for additional food production, heat stress (HS) is a major concern in the livestock industry across all species. Animals experience HS when exposed to high environmental temperatures outside their thermal neutral zone. The level of the effects can vary due to the length and intensity of HS to which the animal is exposed to. In experiment one, laying hens with access to cooled perches during HS had a lower heterophil to lymphocyte ratio compared to the control hens after 4 h of acute heat stress, …

Prediction Of Parturition And Dystocia In Holstein-Friesian Cattle, And Cesarean Section In Dystocic Beef Cattle, Wen Han Mark Hiew

Open Access Dissertations

Dystocia is a major problem in the dairy industry as it causes livestock and economic loss. It is more frequently seen in primiparous cattle compared to their multiparous counterparts due to their smaller stature and the slow maturation of pelvic dimensions. In some instances, human intervention of the parturition process is imperative to avoid pain, injury, and mortality of the neonate and the dam. The ability to accurately predict dystocia and the time of parturition will ensure that timely assistance can be given to animals that are of high risk of dystocia.^ The present thesis contains four studies that explore …

Clinical Investigation Of Plethysmographic Variability Index: A Derivative Index Of Pulse Oximetry In Anesthetized Dogs, Vaidehi V. Paranjape

Open Access Theses

Plethysmographic Variability Index (PVI) is a derivative index of pulse oximetry that allows evaluating an individual's intravascular volume status. Perfusion Index (PI) represents the strength of pulse signal at the anatomic site of measurement from which PVI is calculated using changes in PI over respiratory cycles. Plethysmographic Variability Index has been used to detect hypovolemia and predict fluid responsiveness in mechanically ventilated human patients however, fewer studies are available in spontaneously breathing patients. The use of PVI has not been explored extensively in dogs so far. The goals of this study were to establish a common range for PVI and …

Cut-Off Values For Gait Variables To Detect Forelimb Lameness In Individual Dogs, Jennifer G. Carr

Open Access Theses

The objective of this study was to characterize kinetic and kinematic variables in dogs with forelimb lameness and determine lameness cutoff values of gait variables using ROC analysis with observational gait analysis (OGA) as reference. Twenty client-owned dogs with unilateral lameness were included. Dogs underwent orthopedic exam, including OGA, and instrumented gait analysis (IGA; kinetic and kinematic analysis). Kinetic variables with the highest accuracy were PVF and %WD with an area under the curve (AUC) of 0.73 and 0.92, respectively. Optimal cutoff value for PVF and %WD were ≤ 10.6 (sensitivity 70% and specificity 75%) and ≤ 29.7 (sensitivity 90% …

Pharmacokinetics And Toxicity Of The Novel Oral Demethylating Agent Zebularine In Laboratory And Tumor Bearing Dogs, Christopher Michael Fulkerson

Open Access Theses

The aim of this thesis is to investigate the pharmacokinetics and toxicity of the novel oral demethylating agent zebularine (zeb) in laboratory dogs and tumor bearing dogs. This thesis focuses on the application of this therapeutic strategy in dogs with naturally occurring invasive transitional cell carcinoma (InvTCC), which serve as a relevant model of the human disease.

DNA hypermethylation in the promoter region is a common epigenetic change in cancer that silences tumor suppressor genes. Zeb is an oral cytidine analog that acts as a demethylating agent. Zeb has been investigated extensively in vitro as well as in mice, rodents …

Assessing The Preparedness Of The Veterinary Profession To Communicate With Limited English Proficient Spanish-Speaking Pet Owners, Ruth Ellen Landau

Open Access Dissertations

The purpose of the present study was to assess the preparedness of the veterinary profession to communicate with limited English proficient Spanish-speaking pet owners (LEP SSPOs). Results of surveys with 393 Latino pet owners, 383 small animal veterinarians and staff, and over 2000 veterinary students indicate that interaction between veterinary professionals and LEP SSPOs is not a rare occurrence: Veterinarians in states with large established or fast-growing Latino communities are seeing LEP SSPOs and their pets on a regular basis: 89% of veterinarians have LEP Spanish-speaking clients, over half of these practitioners are seeing LEP SSPOs weekly, and over half …

Navigating Diversity And Inclusion In Veterinary Medicine, Lisa M. Greenhill, Kauline Cipriani Davis, Patricia M. Lowrie, Sandra F. Amass

New Directions in the Human-Animal Bond

This book addresses the continued lack of the diversity in veterinary medicine, the least inclusive of all medical professions. Effective navigation of the complexity of diversity and inclusion in veterinary medicine requires clear enumeration, recognition, and understanding of key issues, challenges, and opportunities. In a nation with rapidly changing demographics, public needs and expectations of the veterinary profession will continue to evolve. A more diverse scientific workforce is required to feed the veterinary profession, not just for the purposed of equity, but as necessity for its sustainability and relevance.The book lays out the history of diversity in the veterinary profession, …

Omega-3 Fatty Acid Supplementation In The Management Of Chronic Lower Airway Inflammatory Conditions In Horses, Nora Nogradi

Open Access Theses

Chronic lower airway inflammatory diseases, such as recurrent airway obstruction (RAO) and inflammatory airway disease (IAD) are common in horses. RAO and IAD present many similarities with asthma in people. Based on studies in human asthma, omega - 3 polyunsaturated fatty acid (PUFA) supplementation has been proposed to improve clinical signs in horses with chronic lower airway inflammatory disease. The purpose of this study was to evaluate the clinical efficacy of an omega-3 PUFA containing equine feed supplement (Aleira^TM) in horses with chronic lower airway disease. The study consisted of two separate experiments. First, a pilot study was …

Mycoplasma Haemocanis – The Canine Hemoplasma And Its Feline Counterpart In The Genomic Era, Naila C. Do Nascimento, Andrea P. Santos, Ana Ms Guimaraes, Phillip J. Sanmiguesl, Joanne Messick

Department of Comparative Pathobiology Faculty Publications

Mycoplasma haemocanis is a hemotrophic mycoplasma (hemoplasma), blood pathogen that may cause acute disease in immunosuppressed or splenectomized dogs. The genome of the strain Illinois, isolated from blood of a naturally infected dog, has been entirely sequenced and annotated to gain a better understanding of the biology of M. haemocanis. Its single circular chromosome has 919 992 bp and a low G + C content (35%), representing a typical mycoplasmal genome. A gene-by-gene comparison against its feline counterpart, M. haemofelis, reveals a very similar composition and architecture with most of the genes having conserved synteny extending over their entire chromosomes …

The Effect Of Infection Order Of Porcine Circovirus Type 2 And Porcine Reproductive And Respiratory Syndrome Virus On Dually Infected Swine Alveolar Macrophages, Yi-Chieh Tsai, Hui-Wen Chang, Chian-Ren Jeng, Tsang L. Lin, Chun-Ming Lin, Cho-Hua Wan, Victor Fei Pang

Department of Comparative Pathobiology Faculty Publications

Abstract Background: Concurrent infection with porcine circovirus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV) is known as one of the major causes for porcine respiratory disease complex (PRDC). Dual infection with PCV2 and PRRSV is consistently to have more severe clinical presentations and pulmonary lesions than infection with PCV2 alone or PRRSV alone. However, it is not known if dual infections with PCV2 and PRRSV in different infection order may lead to different clinical symptoms in the host. To mimic the possible field conditions, swine alveolar macrophages (AMs) were inoculated with PCV2 and PRRSV in vitro …

Let's Cure Cancer!, Wallace Morrison, Jessica Schneider, Sandra F. Amass

College of Veterinary Medicine eBooks

From ancient Egypt to modern times, scientists have been learning new ways to prevent, diagnose, and treat cancer. In this book, you’ll learn about cancer and how veterinarians, veterinary technicians, physicians, and other scientists are working together to find a cure for cancer in people and their pets.

Fat Dogs And Coughing Horses: Be The Vet! Solve The Case!, Kauline Cipriani Davis, Jessica Schneider, Henry Green Iii, Thad Blossom

College of Veterinary Medicine eBooks

This activity book highlights various careers in the veterinary profession and provides veterinary medical cases for the reader to solve.

This project was supported by the National Center for Research Resources and the Division of Program Coordination, Planning, and Strategic Initiatives of the National Institutes of Health through Grant Number R25 OD011115-04.

Also attached are the 6 fitness handouts - Canine Obesity, Dog Weight by Breed, Doggie Fitness, Eating Healthy, Healthy Treats, and Weather Tips.

How I Became A Veterinarian, Kauline Cipriani Davis, Sandra F. Amass, Paula Green

College of Veterinary Medicine eBooks

Veterinarians have fun and exciting jobs! In this book you will meet veterinarians from all over the world. These veterinarians work hard to make sure people and their animals stay healthy. Maybe one day you will be a veterinarian too!

Impact Of Preexisting Adenovirus Vector Immunity On Immunogenicity And Protection Conferred With An Adenovirus-Based H5n1 Influenza Vaccine, Aseem Pandey, Neetu Singh, Sai V. Vemula, Laurent Couetil, Jacqueline M. Katz, Ruben Donis, Suryaprakash Sambhara, Suresh K. Mittal

Department of Comparative Pathobiology Faculty Publications

The prevalence of preexisting immunity to adenoviruses in the majority of the human population might adversely impact the development of adaptive immune responses against adenovirus vector-based vaccines. To address this issue, we primed BALB/c mice either intranasally (i.n.) or intramuscularly (i.m.) with varying doses of wild type (WT) human adenovirus subtype 5 (HAd5). Following the development of immunity against HAd5, we immunized animals via the i.n. or i.m. route of inoculation with a HAd vector (HAd-HA-NP) expressing the hemagglutinin (HA) and nucleoprotein (NP) of A/Vietnam/1203/ 04 (H5N1) influenza virus. The immunogenicity and protection results suggest that low levels of vector …

Zwitterionic Chitosan Derivative, A New Biocompatible Pharmaceutical Excipient, Prevents Endotoxin-Mediated Cytokine Release, Gaurav Bajaj, William G. Van Alstine, Yoon Yeo

Department of Comparative Pathobiology Faculty Publications

Chitosan is a cationic polymer of natural origin and has been widely explored as a pharmaceutical excipient for a broad range of biomedical applications. While generally considered safe and biocompatible, chitosan has the ability to induce inflammatory reactions, which varies with the physical and chemical properties. We hypothesized that the previously reported zwitterionic chitosan (ZWC) derivative had relatively low pro-inflammatory potential because of the aqueous solubility and reduced amine content. To test this, we compared various chitosans with different aqueous solubilities or primary amine contents with respect to the intraperitoneal (IP) biocompatibility and the propensity to induce proinflammatory cytokine production …

Genome Of Mycoplasma Haemofelis, Unraveling Its Strategies For Survival And Persistence, Andrea P. Santos, Ana Ms Guimaraes, Naila C. Do Nascimento, Phillip J. Sanmiguel, Samuel W. Martin, Joanne Messick

Department of Comparative Pathobiology Faculty Publications

Mycoplasma haemofelis is a mycoplasmal pathogen (hemoplasma) that attaches to the host’s erythrocytes. Distributed worldwide, it has a significant impact on the health of cats causing acute disease and, despite treatment, establishing chronic infection. It might also have a role as a zoonotic agent, especially in immunocompromised patients. Whole genome sequencing and analyses of M. haemofelis strain Ohio2 was undertaken as a step toward understanding its survival and persistence. Metabolic pathways are reduced, relying on the host to supply many of the nutrients and metabolites needed for survival. M. haemofelis must import glucose for ATP generation and ribose derivates for …

Comparison Of Genomic And Proteomic Data In Recurrent Airway Obstruction Affected Horses Using Ingenuity Pathway Analysis, Julien Racine, Vinzenz Gerber, Marybeth Miskovic Feutz, C Paige Riley, Jiri Adamec, June E. Swinburne, Laurent Couetil

Department of Comparative Pathobiology Faculty Publications

Background: Recurrent airway obstruction (RAO) is a severe chronic respiratory disease affecting horses worldwide, though mostly in the Northern hemisphere. Environmental as well as genetic factors strongly influence the course and prognosis of the disease. Research has been focused on characterization of immunologic factors contributing to inflammatory responses, on genetic linkage analysis, and, more recently, on proteomic analysis of airway secretions from affected horses. The goal of this study was to investigate the interactions between eight candidate genes previously identified in a genetic linkage study and proteins expressed in bronchoalveolar lavage fluid (BALF) collected from healthy and RAO-affected horses. The …

Complete Genome Sequence Of Mycoplasma Suis And Insights Into Its Biology And Adaption To An Erythrocyte Niche, Ana Ms Guimaraes, Andrea P. Santos, Phillip Sanmiguel, Thomas Walter, Jorge Timenetsky, Joanne Messick

Department of Comparative Pathobiology Faculty Publications

Mycoplasma suis, the causative agent of porcine infectious anemia, has never been cultured in vitro and mechanisms by which it causes disease are poorly understood. Thus, the objective herein was to use whole genome sequencing and analysis of M. suis to define pathogenicity mechanisms and biochemical pathways. M. suis was harvested from the blood of an experimentally infected pig. Following DNA extraction and construction of a paired end library, whole-genome sequencing was performed using GS-FLX (454) and Titanium chemistry. Reads on paired-end constructs were assembled using GS De Novo Assembler and gaps closed by primer walking; assembly was validated by …

Information-Seeking Behaviors Of First-Semester Veterinary Students: A Preliminary Report, Sharon A. Weiner, Gretchen Stephens, Abdelfattah Y.M. Nour

Libraries Faculty and Staff Scholarship and Research

Although emphasis in veterinary education is increasingly being placed on the ability to find, use, and communicate information, studies on the information behaviors of veterinary students or professionals are few. Improved knowledge in this area will provide valuable information for course and curriculum planning and the design of information resources. This article describes a survey of the information-seeking behaviors of first-semester veterinary students at Purdue University. A survey was administered as the first phase of a progressive semester-long assignment for a first semester DVM course in systemic mammalian physiology. The survey probed for understanding of the scientific literature and its …

The P53 Inhibitor Mdm2 Facilitates Sonic Hedgehog- Mediated Tumorigenesis And Influences Cerebellar Foliation, Reem Malek, Jennifer Matta, Natalie Taylor, Mary Ellen Perry, Susan Mendrysa

Department of Basic Medical Sciences Faculty Publications

Disruption of cerebellar granular neuronal precursor (GNP) maturation can result in defects in motor coordination and learning, or in medulloblastoma, the most common childhood brain tumor. The Sonic Hedgehog (Shh) pathway is important for GNP proliferation; however, the factors regulating the extent and timing of GNP proliferation, as well as GNP differentiation and migration are poorly understood. The p53 tumor suppressor has been shown to negatively regulate the activity of the Shh effector, Gli1, in neural stem cells; however, the contribution of p53 to the regulation of Shh signaling in GNPs during cerebellar development has not been determined. Here, we …