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The Effects Of Prostatic Fluid On Functional Characteristics Of Cooled Canine Semen, Reto Fritsche
The Effects Of Prostatic Fluid On Functional Characteristics Of Cooled Canine Semen, Reto Fritsche
LSU Master's Theses
The objectives of this study were to investigate concentration dependent effects of canine prostatic fluid (PF) on in vitro seminal parameters of cooled canine semen. Sperm motility parameters, plasma membrane integrity and stability, acrosome integrity and DNA fragmentation were measured after the addition of 0%, 10%, 25%, or 50% PF to extended semen of fertile dogs. Assessments were made at 0 h pre-cooling, at 24, and 48 h of cooled storage (4 °C), and after freezing and thawing followed by incubation (37 °C) at 0, 4 and 24 h. Our hypothesis was that lower dilutions of canine semen with PF …