Veterinary Medicine Commons^™

Accuracy Of A Sacral Drilling With A Custom 3d Printed Drilling Guide Or Free-Hand Technique In Canine Experimental Sacroiliac Luxation, Daniel Mccarthy

LSU Master's Theses

ABSTRACT

The objective of this study was to improve the accuracy of drilling during the repair of sacroiliac luxation with a 3D-printed patient-specific drill guide (3D-GDT) compared to free-hand drilling technique (FHDT). A blinded, randomized, prospective study was performed including sixteen canine cadavers (20-25 kg) euthanized for reasons not related to the study. Dorsal, bilateral, sacroiliac luxations (SILs) were created experimentally. A pelvic CT was performed pre- and post-drilling. The FHDT was drilled followed by 3D-GDT for each sacrum. CT and 3D measurements of craniocaudal and dorsoventral angles were compared between FHDT and 3D-GDT, as well as deviations of entry …

The Effects Of Prostatic Fluid On Functional Characteristics Of Cooled Canine Semen, Reto Fritsche

LSU Master's Theses

The objectives of this study were to investigate concentration dependent effects of canine prostatic fluid (PF) on in vitro seminal parameters of cooled canine semen. Sperm motility parameters, plasma membrane integrity and stability, acrosome integrity and DNA fragmentation were measured after the addition of 0%, 10%, 25%, or 50% PF to extended semen of fertile dogs. Assessments were made at 0 h pre-cooling, at 24, and 48 h of cooled storage (4 °C), and after freezing and thawing followed by incubation (37 °C) at 0, 4 and 24 h. Our hypothesis was that lower dilutions of canine semen with PF …

Optimization Of Feline Adipose-Derived Multipotent Stromal Cell Isolation And Canine Cranial Cruciate Ligament Regeneration With Intra-Articular Adipose-Derived Multipotent Stromal Cells, Nan Zhang

LSU Doctoral Dissertations

Adult multipotent stromal cells (MSCs) have become popular within veterinary sciences for direct administration and tissue regeneration due to their differentiation, trophic and immunosuppressive properties. However, current isolation and expansion techniques provide a heterogeneous population with other types of cells, which may affect the efficiency and efficacy of treatments. The progenitor properties and function need to be further evaluated in vitro prior to in vivo application. The first part of this dissertation was to identify the optimal MSC source for general canine orthopedic applications such as intra-articular injection and joint tissue regeneration. Based on side-by-side comparisons of cell doublings, colony …

Computed Tomographic Assessment Of Canine Arytenoid Lateralization, James Richard Wignall

LSU Master's Theses

Unilateral arytenoid lateralization is a commonly performed surgical treatment for laryngeal paralysis in dogs. It involves fixing the moveable arytenoid cartilage to the thyroid (TAL) or cricoid (CAL) cartilage or both (CTAL). This increases the area of the rima glottidis (RGA), to allow reduced airway pressure and laryngeal resistance in vitro and ameliorates clinical signs in vivo. It may also increase the patient’s predisposition for aspiration pneumonia, which occurs in around 20% of clinical patients. No surgical technique has been correlated with clinical outcome or risk of aspiration pneumonia. Objective analysis of the effects of surgery on the three dimensional …

Phenotypic Characterization Of Canine Cranial Cruciate Ligament Associated Synoviocytes, Sunil C. Vasanjee

LSU Master's Theses

Identification of synoviocytes surrounding the canine cranial cruciate ligament (CrCL) has not been investigated. Objectives 1) Develop and validate a technique to identify and quantify normal canine CrCL associated synoviocytes. 2) Compare synoviocyte phenotype proportions surrounding normal and abnormal canine CrCLs Design In vitro experimental Animals Cranial cruciate ligaments from 4 intact female and 6 intact adult male mixed-breed dogs (objective 1) and from 8 adult female hound dogs (objective 2) Methods Objective 1) Normal CrCLs - CD18 and HSP25 epitopes were colocalized using immunohistochemistry. Sagittal sections were quantified in the proximal, middle, and distal aspects of each section. Western …

Urokinase And Urokinase Receptor In The Urinary Tract Of The Dog, Trina Racquel Bailey

LSU Master's Theses

Urokinase (uPA) and urokinase receptor (uPAR) are present in the urinary tract of people, mice, rats and cows. The presence of urokinase and urokinase receptor in the urinary tract of dogs has not been documented. Documenting the presence of uPA and uPAR in the urinary tract of healthy dogs will help determine the role these proteins play in pathology of the urinary tract in further research. Urine was collected from fifty-four healthy dogs. A fluorescence assay was developed using the fluorogenic substrate Z-Gly-Gly-Arg-AMC, HCl. This assay documented presence of active uPA-like protein in concentrated urine. A direct sandwich ELISA assay …

Immunological, Hematological, And Serum Biochemical Effects Of High Level Dietary Fish Oil And Vitamin E Supplementation In The Dog, Casey J. Leblanc

LSU Doctoral Dissertations

Inflammation is a component of the innate immune response. However, severe or prolonged inflammation can be detrimental. Dietary n-3 fatty acid supplementation has proven clinical benefits in chronic inflammatory diseases, most likely due to reduced synthesis of inflammatory mediators and inhibition of lymphocyte proliferation and function. The purposes of this study were to characterize alterations in inflammatory mediator production, and lymphocyte proliferation, in dogs fed a diet consisting of 0.65% n-3 fatty acids (DMB) with an n-6:n-3 fatty acid ratio of 3.4:1. Fifteen dogs were randomly assigned to one of three dietary groups for twelve weeks. Group Sunflower oil received …