Open Access. Powered by Scholars. Published by Universities.®

Veterinary Medicine Commons™

Open Access. Powered by Scholars. Published by Universities.®

Louisiana State University

2007

Articles 1 - 10 of 10

Full-Text Articles in Veterinary Medicine

Influence Of Tumor Necrosis Factor-Alpha And Minocycline On Microglia And Macrophage Activation During Polytropic Retrovirus Infection, Meryll E. Corbin Jan 2007

Influence Of Tumor Necrosis Factor-Alpha And Minocycline On Microglia And Macrophage Activation During Polytropic Retrovirus Infection, Meryll E. Corbin

LSU Master's Theses

Microglia/macrophage activation has been associated with the pathogenesis of various neurological diseases including human immunodeficiency virus encephalitis, transmissible spongiform encephalitis, and Alzheimer's disease (AD). In vitro studies have indicated a role for TNFα in activating these cells which leads to their migration, proliferation, and secretion of proinflammatory cytokines and chemokines that may potentially damage brain tissue. In the current study, we analyzed the phenotype of microglia and macrophages enriched from wild type and TNFα deficient mice infected with a neurovirulent murine retrovirus. Although TNF receptors CD120a and CD120b were expressed on both microglia and macrophage population, unaltered by either retrovirus …

Go to article

Brucella Melitensis: The Evaluation Of A Putative Hemagglutinin Gene's Effect On Virulence In The Caprine Model, Quinesha Laticia Perry Jan 2007

Brucella Melitensis: The Evaluation Of A Putative Hemagglutinin Gene's Effect On Virulence In The Caprine Model, Quinesha Laticia Perry

LSU Doctoral Dissertations

Brucella melitensis is a facultative intracellular bacterial pathogen that causes abortions in goats and sheep and Malta fever in humans. The zoonotic disease brucellosis causes severe economic losses in the Mediterranean region and parts of Africa, Asia, and Latin America. With the completion of the genomic sequences of B. abortus 2308 and B. melitensis 16M, no classical virulence factors were found; and the chromosomes were virtually identical. However, in B. melitensis, a putative hemagglutinin gene was identified which is absent in B. abortus. The possibility of the hemagglutinin gene being a potential virulence factor was evaluated via gene replacement/deletion in …

Go to article

Genetics Of Herpes Simplex Virus Type-1 Tegument Proteins Involved In Virion Morphogenesis And Egress, Preston A. Fulmer Jan 2007

Genetics Of Herpes Simplex Virus Type-1 Tegument Proteins Involved In Virion Morphogenesis And Egress, Preston A. Fulmer

LSU Doctoral Dissertations

Herpes simplex virus type-1 (HSV-1) morphogenesis occurs in multiple stages within infected cells. Initially, the virion capsid assembles within the nucleus and buds through the nuclear membrane into the cytoplasm. Within the cytoplasm, additional tegument proteins attach to the capsid and the fully tegumented capsids bud into trans-Golgi network (TGN) derived vesicles. Enveloped virions are ultimately secreted to extracellular spaces. The process by which the cytoplasmic capsids bud into TGN-derived vesicles is not well understood. The prevalent model calls for specific interactions among viral tegument proteins and membrane proteins and glycoproteins embedded within TGN membranes. To further investigate the roles …

Go to article

Carprofen Compromises The Integrity And Barrier Function Of The Colonic Mucosa Of The Dog, Catherine Alix Briere Jan 2007

Carprofen Compromises The Integrity And Barrier Function Of The Colonic Mucosa Of The Dog, Catherine Alix Briere

LSU Master's Theses

Effects of carprofen on colon of dog have not been investigated. Objectives 1) Measure conductance and permeability to mannitol of transverse, proximal descending and distal descending colonic mucosa of dog. 2) Measure conductance and permeability to mannitol of colonic mucosa of dog in presence of carprofen. Design In vitro experimental - nested, randomized block design Animals Colonic mucosa from 6 (objective 1) and 7 (objective 2) mature mixed-breed dogs. Methods Objective 1) Control - Three sections of mucosa from each region of colon were mounted in Ussing chamber units. Conductance was calculated every 15 min for 240 min. Flux of …

Go to article

Evaluating The Effect Of Two Commercial Antimicrobial Products On Salmonella Spp. In The Aquatic Habitat Of The Red-Eared Slider Turtle (Trachemys Scripta Elegans), Trevor Theadore Zachariah Jan 2007

Evaluating The Effect Of Two Commercial Antimicrobial Products On Salmonella Spp. In The Aquatic Habitat Of The Red-Eared Slider Turtle (Trachemys Scripta Elegans), Trevor Theadore Zachariah

LSU Master's Theses

Turtle-associated salmonellosis was recognized as a public health concern in the 1960’s, particularly due to an increase in the incidence of disease among children. In response to the public health threat, the United States Food and Drug Administration (FDA) implemented regulations in 1975 restricting the sale of turtle eggs and turtles with a carapace length less than 10.2 cm. Since that time, attempts to eliminate Salmonella from turtles using antibiotics have been unsuccessful and lead to antibiotic resistant strains of Salmonella on turtle farms. Recent work has focused on identifying non-antibiotic products to suppress or eliminate Salmonella and reverse the …

Go to article

Lymphohistiocytic Proliferative Syndrome Of Alligators (Alligator Mississippiensis): A Cutaneous Manifestation Of West Nile Virus, Javier G. Nevarez Jan 2007

Lymphohistiocytic Proliferative Syndrome Of Alligators (Alligator Mississippiensis): A Cutaneous Manifestation Of West Nile Virus, Javier G. Nevarez

LSU Doctoral Dissertations

ABSTRACT In 1999, there were reports of a new type of lesion in the hides of captive reared alligators from Florida. Similar lesions were first reported from alligator hides in Louisiana in 2001; however, it wasn’t until 2002 that small epizootics became apparent. In 2002, the Louisiana Department of Wildlife and Fisheries began a collaborative effort with the Louisiana State University School of Veterinary Medicine (LSU SVM) to help elucidate the etiology of “PIX” disease, later renamed Lymphohistiocytic Proliferative Syndrome of Alligators (LPSA). Preliminary work concluded that LPSA was a systemic disease affecting multiple tissues. Based on the results of …

Go to article

Evaluation Of Selected Immune Response To Haemonchus Contortus In Gulf Coast Native Compared To Suffolk Lambs, Krishna P. Shakya Jan 2007

Evaluation Of Selected Immune Response To Haemonchus Contortus In Gulf Coast Native Compared To Suffolk Lambs, Krishna P. Shakya

LSU Doctoral Dissertations

Haemonchus contortus is one of the major nematode parasites causing substantial economic losses in small ruminant farming worldwide. Recently, effect of anthelmintic treatment has decreased due to an increasing problem of nematode populations that have developed resistance to anthelmintics. There are certain breeds of sheep that are identified as being relatively resistant to the parasite including Gulf Coast Native (Native) sheep. Understanding the mode of immune response that helps these breeds of sheep control infection could help design vaccines and enhance control programs. This experiment was designed to evaluate the immunological responses of Native, compared with susceptible Suffolk sheep that …

Go to article

Characterization Of A Virulence Related Hypothetical Protein In Edwardsiella Ictaluri, Ildiko Katalin Polyak Jan 2007

Characterization Of A Virulence Related Hypothetical Protein In Edwardsiella Ictaluri, Ildiko Katalin Polyak

LSU Master's Theses

Although the biochemical characterization of E. ictaluri, the subsequent disease progression of enteric septicemia of catfish (ESC), and the associated pathologic lesions are well characterized, the mechanism of invasion of E. ictaluri into a susceptible host is poorly understood. Identification and confirmation of virulence factors and associated genes of E. ictaluri is crucial to elucidating the pathogenesis of this important disease. A signature tagged mutagenesis (STM) study conducted by Thune et al. (2006) identified 50 E. ictaluri clones with transposon insertions in genes potentially involved in pathogenesis. A specific STM mutant, 233PR, carrying a transposon insertion in a gene encoding …

Go to article

Depletion Of 32-Kbp Circular Plasmids From Borrelia Burgdorferi, Amanda Paige Derouen Polito Jan 2007

Depletion Of 32-Kbp Circular Plasmids From Borrelia Burgdorferi, Amanda Paige Derouen Polito

LSU Master's Theses

The Lyme disease spirochete Borrelia burgdorferi has a very unusual genome composed of one linear chromosome and up to 21 linear and circular plasmids. Several plasmids are known to be important either for mammalian infection or tick colonization. A single spirochete harbors up to 7 different cp32 plasmids; however, nothing is known about their role in mammalian infection. The plasmids in this family are well maintained during in vitro cultivation, making it difficult to study their functions. To effectively deplete the plasmids, an 8kbp fragment containing essential elements for replication and partitioning in B. burgdorferi was amplified from one of …

Go to article

The Role Of Toll-Like Receptor 7 In The Neuropathogenesis Of Retrovirus Infection In Neonates, Stephanie Diane Lewis Jan 2007

The Role Of Toll-Like Receptor 7 In The Neuropathogenesis Of Retrovirus Infection In Neonates, Stephanie Diane Lewis

LSU Master's Theses

Viral infections of the central nervous system (CNS) in infants are rare; however, they are associated with high morbidity and mortality rates. These virus infections often induce strong innate immune responses in the brain including: the production of cytokines and chemokines, the activation of astrocytes and microglia and the recruitment of macrophages. Innate immune responses are often initiated by toll-like receptors (TLR). Several studies have demonstrated that toll-like receptor 7 (TLR7) can be stimulated by single-stranded RNA from multiple viruses. In the current study, we examined the mechanism by which TLR7 contributes to neuroinflammation in the neonatal brain using a …

Go to article