Veterinary Medicine Commons^™

Effect Of Rosmarinic Acid On Dog Semen Quality After Freeze-Drying By Applying Freeze-Drying Machines For Vaccine Production, Janjira Phavaphutanon, Phannipa Phungsombun, Kornchai Kornkaewrat, Sudtisa Laopiem, Wilairat Chumsing, Pattra Moonjit

The Thai Journal of Veterinary Medicine

Freeze-drying (FD) is a sperm preservation method that eliminates the need for liquid nitrogen. However, the FD process can expose sperm to free radicals and temperature variations. This study aimed to determine the impact of rosmarinic acid (RA) as an antioxidant, initial sperm motility, and storage temperature on the DNA integrity of freeze-dried dog sperm. Forty-four ejaculates from 15 dogs of various breeds were divided into control (FD extender) and RA (FD extender with RA) groups, further categorized by motility (above or below 70%). After FD, samples were stored for three months at room temperature and 4 °C. Sperm quality …

Relationship Of Hyperlipidemia And Gallbladder Sludge Scores In Dogs With Hyperadrenocorticism, Jayeop Jo, Dohee Lee, Taesik Yun, Yoonhoi Koo, Yeon Chae, Dongwoo Chang, Byeong-Teck Kang, Mhan-Pyo Yang, Kyung-Duk Min, Hakhyun Kim

The Thai Journal of Veterinary Medicine

Hyperadrenocorticism (HAC) and hyperlipidemia have been identified as the causes of cholestatic disease in dogs. However, the possibility of direct association of HAC with a cholestatic disease or secondary hyperlipidemia-induced cholestatic disease in dogs with HAC has not been elucidated. This study aimed to evaluate the differences in serum triglyceride (TG), total cholesterol (TC) and basal cortisol (BC) concentrations based on the gallbladder (GB) sludge score in dogs with HAC. This study was a retrospective cohort study. In total, 36 dogs with pituitary-dependent hyperadrenocorticism (PDH) were divided into six groups (GB scores 0–5) based on the ultrasonographic evaluation of the …

Effects Of Prostaglandin Administration After Farrowing On Milk Output, Vaginal Discharge, And Mastitis During The Early Postpartum Period In Sows Under Field Conditions, G. Romano, R. Saleri, F. De Rensis, E. Dall’Olio, Padet Tummaruk, M. Andrani, C. Mazzoni

The Thai Journal of Veterinary Medicine

The present study evaluates the clinical effects of early post-partum prostaglandin administration on milk output, vaginal discharge and mastitis in sow. Two groups of sows were used: sows (n = 25) treated with prostaglandin F-2alpha (PGF-2α) intramuscularly 36 h after farrowing and a control group (n = 48) that did not receive any treatment. Seventy-two hours after farrowing, all animals were scored for rectal temperature, respiratory rate, feed ingestion, milk output, vaginal discharge and mastitis. Rectal temperature, feed intake, and milk output did not differ between the groups (P = 0.43). Regarding the clinical data, vaginal discharge was detected in …

Symmetric Dimethylarginine In Dogs With Pulmonary Arterial Hypertension, Siwayu Rattanakanokchai, Numfa Fungbun, Ketmanee Senaphan, Supranee Jitpean, Trasida Ployngam

The Thai Journal of Veterinary Medicine

Pulmonary arterial hypertension (PAH) causes impaired kidney function in humans. However, this occurrence has not been well investigated in PAH dogs. Serum symmetrical dimethylarginine (SDMA) is one of the early biomarkers of kidney impairment. The present study aims to evaluate SDMA levels of first diagnosed PAH (n=14) compared with healthy dogs (n=12) and to analyze the correlation between SDMA levels and significant echocardiographic parameters of PAH dogs. The mean SDMA of PAH dogs was statistically higher than that of healthy dogs (P = 0.003), while the mean serum creatine level had no difference among groups (P = 0.456). Precapillary PAH …

Normal Thymus Evaluation Using Radiographs And Computed Tomographic Images In Young And Adult Dogs, Yoenhee Park, Sang-Kwon Lee, Sooyoung Choi, Hojung Choi, Youngwon Lee, Kija Lee

The Thai Journal of Veterinary Medicine

The thymus generally presents as a sail-shaped soft-tissue opacity on thoracic radiographs in young, pre-pubescent dogs. After puberty, lymphatic tissue is replaced by adipose tissue and can usually no longer be identified on radiographs; however, thymic remnants have often been observed in adult dogs. The objective of this retrospective descriptive study was to investigate the incidence of thymic remnants using thoracic radiographs and to analyze the incidence in dogs by age, sex and breed. We analyzed the data from 1,464 dogs that underwent both thoracic ventrodorsal and right lateral radiography, of which 40 underwent both radiography and computed tomography (CT). …

Development Of Wireless Device Prototype For Measurement Of Rumen Ph And Temperature Continuously In Cattle, Piyanat Prasomsri, Apirak Tudsorn, Aekaluck Leklerdsiriwong, Chaidate Inchaisri, Siraphop Tooprakai

The Thai Journal of Veterinary Medicine

This study aims to develop a prototype rumen pH and rumen temperature measuring device for continuous measurement. The benefit is for the monitoring and early diagnosis of metabolic diseases in cattle, especially rumen acidosis and subacute ruminal acidosis. The developed prototype device was approximately 50 millimeters in diameter, 145 millimeters in length and weighed 400 grams. The outer structure of the prototype was made from abs plastic made by a 3D printer, while the internal device contained pH and temperature sensors, a microcontroller and battery. The prototype was placed in the ventral sac of the rumen of fistulated cattle for …

Qualitative Risk Analysis On Likelihood Of Foot And Mouth Disease (Fmd) Reintroduction In The Philippines Through The Importation Of Dairy Cattle From Fmd-Free Zones With Vaccination In Brazil, Emerson P. Tapdasan, Kenny Oriel A. Olana

The Thai Journal of Veterinary Medicine

The potential of Girolando cattle from Brazil has generated much interest in the Philippines and the possibility of their importation to contribute to the latter’s dairy industry development has been raised. The most crucial issue that needs to be addressed is the probable introduction of exotic livestock diseases or pathogens of different strains like Foot-and-Mouth Disease virus (FMDv) which might destroy the Philippines’ livestock industry. This study involved only the work required to conduct a preliminary qualitative risk analysis (RA) on the likelihood of FMDv being reintroduced into the Philippines through the importation of Girolando cattle from FMD-free zones with …

Comparison Of Different Pinning Techniques For 3d-Printed Radius With Salter-Harris Type I Fracture, Tea-Hyung Kim, Hwi-Yool Kim

The Thai Journal of Veterinary Medicine

The purpose of the present study was to compare different pinning techniques for distal radius with Salter-Harris type I fracture. Computed Tomography (CT) images of a distal antebrachium of the 6.5 kg, growing, 6-month-old intact male mongrel dog were 3D-printed with Polycarbonate (PC) material. Twenty-one synthetic bones were classified into three groups. Cross pinning, Parallel pinning, and HK2 pinning techniques were applied to each group. A compressive shear test was applied to each group, and the results at 1 mm, 2 mm, and 3 mm displacement and the load at failure were analyzed through statistical software. It was found that …

Histories, Cultural Contexts, Health Status, Wildlife Contact Characteristics, And Perception Towards Covid-19 Of The Urak Lawoi Ethnic Group In Koh Lanta District, Krabi Province Of Thailand: A Qualitative Study, Kanokwan Suwannarong, Paisit Boonyakawee, Thanomsin Ponlap, Kannika Thammasutti, Phitsanuruk Kanthawee, Nisachon Bubpa, Chularat Boonyakawee, Vittaya Pulkird, Junsri Changnum

Journal of Health Research

Background: The Urak Lawoi ethnic group is an indigenous community residing in the Andaman Sea of southern Thailand and are engaged in various activities related to wild animals. This qualitative study was conducted from November 2022 to February 2023 to collect information on wildlife contact characteristics and determine the level of awareness and understanding of zoonotic diseases and COVID-19 risks among this ethnic group in Koh Lanta district, Krabi province.

Methods: This qualitative study collected data through key informant interviews (KIIs) and focus group discussions (FGDs). The study respondents included 29 Urak Lawoi village health volunteers (VHVs), To Mo …

Distribution Pattern And Risk Factors Of Foot And Mouth Disease Outbreaks In Java Island, Indonesia, Virgilius Martin Kelake Kedang

Chulalongkorn University Theses and Dissertations (Chula ETD)

Indonesia experienced new outbreaks of foot and mouth disease (FMD) in 2022 after being officially free from the disease for several decades. The outbreak started in April 2022 in East Java and rapidly spread to many regions in Indonesia. This study aimed to investigate the distribution pattern and risk factors associated with the FMD outbreaks in Java Island, Indonesia, from 2022 to 2023. Descriptive, spatial, spatiotemporal, and risk factor analyses were employed to examine the outbreak dynamics and contributing factors. The results indicated that the outbreaks were distributed across the island, with East Java being the most affected region. The …

Epidemiological And Molecular Studies On Lumpy Skin Disease Outbreaks In Beef Cattle In Thailand During 2021-2022, Phurida Sripipattanakul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Lumpy skin disease (LSD) is a vector-borne viral disease of cattle caused by the Lumpy skin disease virus (LSDV). LSD is considered as an emerging infectious disease in cattle in Asia and poses a significant threat to cattle production across Africa, Europe, and Asia. This study aimed to describe the epidemiological characteristics of LSD outbreaks in beef cattle in Thailand and to conduct a molecular survey of LSDV infection in beef cattle from May 2021 to December 2022. The study included 594 beef cattle from 257 farms affected by the LSD outbreaks in 28 provinces. We collected hair samples (n=492) …

Comparative Study Of The Effects Of Dietary Supplements On Reproductive And Growth Performance In Sows And Nursery Pigs, Panumas Kongpanna

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study evaluated the effects of dietary supplementation with a multi-strain probiotic containing Bacillus subtilis (5 × 107 CFU/g) and Lactobacillus plantarum (1 × 109 CFU/g) on reproductive performance in multiparous sows and growth performance in weaned piglets. In Experiment 1, 300 sows were assigned to control or probiotic diets across five parity groups (2, 3, 4, 5 and 6 to 9) using a 2 × 5 factorial design. Experiment 2 involved 120 weaned piglets randomly allocated to four dietary treatments: control, antibiotic, enzyme, or probiotic. Measurements included reproductive and litter performance, colostrum IgG, milk composition, and piglet growth, digestibility, …

Strategies Of Utilizing Sex-Sorted Semen To Improve Beef Cattle Herd Production And Using Lasia Spinosa Thw. And Zinc Supplementation To Improve Semen Quality Of Bulls And Boars With Poor Semen Profile, Supitcha Kaewma

Chulalongkorn University Theses and Dissertations (Chula ETD)

The objectives of the thesis were i) to improve the success of using sex-sorted semen and ii) to enhance the semen quality in low semen quality bulls and boars. Four experiments were conducted to test the hypothesis as 1) on-farm factors influence the success of sexed semen artificial insemination (AI) in cattle herds, 2) estrus synchronization protocols can be integrated in sex-sorted semen AI in cattle herd, 3) Lasia spinosa Thw. (LST) supplementation for improving semen quality in bull and boar sires with poor semen quality, 4) zinc supplementation for improving semen quality in subfertile boar. Study 1: Experiment 1, …

Efficacy Of Bacillus-Based Probiotic And Nspase Supplementation On Growth Performance And Gut Health Of Broilers Fed High Level Of Palm Kernel Meals Under High Stocking Density, Nurul Imawati

Chulalongkorn University Theses and Dissertations (Chula ETD)

Palm kernel meal (PKM) is a by-product of the oil palm industry with significant potential in poultry feed, although its high content of anti-nutritional factors limits its utilization. It is hypothesized that dietary supplementation with fiber-degrading enzymes and probiotics may enhance the use of PKM. This study investigated the effects of Bacillus-based probiotics (PRO) and non-starch polysaccharide enzymes (NSPase, mannanase, and xylanase) on broiler performance and gut health when fed a high level of PKM under high stocking density (HSD, Experiment 1) and normal stocking density (NSD, Experiment 2). In Experiment 1, 4,480 mixed-sex Ross308 broilers were allocated to eight …

The Development Of A Lateral Flow Immunochromatographic Strip Test For Evaluation Of Specific Antibody Levels Against Porcine Epidemic Diarrhea Virus In Pig Milk, Patumporn Jermsutjarit

Chulalongkorn University Theses and Dissertations (Chula ETD)

Porcine epidemic diarrhea virus (PEDV) causes severe watery diarrhea in pigs of all ages, but it is particularly devastating in neonatal piglets, where it can result in 100% mortality. Therefore, lactogenic immunity is crucial for protecting against PEDV infection and reducing its virulence. One method for controlling PEDV outbreaks involves using feedback as a protocol, which entails inoculating pregnant sows with infected-PEDV intestines from piglets to enhance lactogenic immunity against PEDV. Interestingly, high serial passage of PEDV has been shown to reduce the virus's virulence. To understand the effects of serial passage, the PEDV was serially passaged both in vitro …

The Development Of A Differentiating Of Infected From Vaccinated Animals (Diva) Elisa To Detect Antibody Against Senecavirus A, Parin Watcharavongtip

Chulalongkorn University Theses and Dissertations (Chula ETD)

Senecavirus A (SVA) is the causative agent of porcine idiopathic vesicular disease (PIVD), a condition clinically indistinguishable from other vesicular diseases in pigs, such as foot and mouth disease (FMD). This similarity complicates differential diagnosis, impacting the global swine industry, including Thailand. Rapid and accurate diagnostic methods are essential for effective disease control. Therefore, developing a highly sensitive and specific diagnostic tool for detecting SVA infection is crucial for distinguishing it from other vesicular diseases and controlling its spread. In this study, we conducted a retrospective analysis of SVA emergence in diagnostic samples from 2010 to 2021 in Thailand, with …

Development Of An Endometrial Cell Culture System Of Thai Swamp Buffalo (Bubalus Bubalis) For Studying The Endometrial-Embryo Interactions, Ruchikon Jongsuwanwattana

Chulalongkorn University Theses and Dissertations (Chula ETD)

Developing and optimizing endometrial sampling techniques are critical for advancing reproductive management and research in cattle and swamp buffalo. This PhD thesis aims i) to design a novel endometrial sampling device for cows and swamp buffaloes, ii) to develop a minimally invasive endometrial cell sample collection technique in cows and buffaloes to prepare endometrial cell culture, iii) to validate the developed mixed endometrial cell culture system retrieved by brush cytology techniques in cows and swamp buffaloes and iv) to utilize the minimally invasive endometrial cell sampling technique for investigation of reproductive disease in ruminant herds. The research is consequently structured …

Proteomic Analysis Of Saliva, Serum And Tissue In Feline Mammary Tumors Using Maldi-Tof Mass Spectrometry And Lc-Mass Spectrometry/Mass Spectrometry, Weejarin Paphussaro

Chulalongkorn University Theses and Dissertations (Chula ETD)

Feline mammary carcinomas (FMC) are commonly found in female cats. Early detection is necessary to prevent both local and distant metastasis and increase survival times. However, the current protein biomarkers of FMC are limited. Several biomarkers of cancers have been discovered by mass spectrometry (MS)-based proteomics, such as matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and liquid chromatography-mass spectrometry/ mass spectrometry (LC-MS/MS) which high sensitivity and accuracy for identified biomarker in various cancer. The purpose of this study was to identify protein expression in saliva, serum and tissue in FMC using MALDI-TOF MS and LC-MS/MS. Results of …

Molecular Characterization And Immunohistochemical Investigation Using Anti-Capsid Protein Antibody Of Canine Circovirus Associated With Respiratory Diseases In Thai Dogs, Wichan Dankaona

Chulalongkorn University Theses and Dissertations (Chula ETD)

Canine circovirus (CanineCV) is an emerging virus linked to various diseases in dogs, especially respiratory and gastrointestinal ailments. This thesis focuses on molecular characterization and histopathological investigation of CanineCV in dogs from Thailand, aiming to provide insights into the role of virus in canine respiratory diseases. The study involved genomic-based surveillance of CanineCV from nasal and oral swabs of infected dogs by employing polymerase chain reaction (PCR) assay. Additionally, the in situ hybridization (ISH) technique was used to identify and localize the viral genome within infected tissues. The CanineCV recombinant capsid gene (rCap) and protein (rCap) were synthesized and characterized, …

Impacts Of Pcv3 And Pcv2 On Reproductive Performance In The Vietnamese Industrial Sow Herds, Tien Tran Nam

Chulalongkorn University Theses and Dissertations (Chula ETD)

Porcine circovirus (PCV) 2 is a globally prevalent virus causing negative impacts on the swine industry. Controlling PCV2 with vaccines is a mandatory requirement in the farming procedures depending on the prevalence of the virus and farm management system including vaccination program. It should be noted that PCV2d management in the studied farms appeared under control via the effective vaccination program. Since the first descriptions of PCV3 in the US, many publications on the viral pathogenesis and effects on susceptible animals have been conducted, but complete consensus has not been reached. Therefore, an alternative approach to studying the virus is …

Use Of Ultrasonography For Evaluation Of Response To Chemotherapy In Canine Multicentric Lymphoma, Somchin Sutthigran

Chulalongkorn University Theses and Dissertations (Chula ETD)

Canine multicentric lymphoma (CML) is a common hematopoietic neoplasm in dogs that initially responds well to treatment but often relapses due to chemotherapy resistance. Evaluating the treatment response is a critical factor in helping veterinary oncologists plan and adjust chemotherapeutic protocols. This study aims to investigate the use of basic diagnostic imaging techniques, such as ultrasound (US), advanced imaging, such as computed tomography (CT) and hematologic and blood chemistry parameters, to enhance the evaluation of treatment response for this disease. This prospective observational study included 95 lymphomatous lymph nodes (LLNs) from 15 dogs with CML and 60 normal lymph nodes …

Vaccination Against Streptococcus Iniae And Bacillus Probiotic For Health Enhancement In Juvenile Barramundi (Lates Calcarifer), Pornpawit Tanpichai

Chulalongkorn University Theses and Dissertations (Chula ETD)

Juvenile Asian seabass (Lates calcarifer) with an average body weight of 10 ± 0.7 g were used to investigate the efficacy of an inactivated Streptococcus iniae vaccine and the probiotic Bacillus subtilis. Fish were intraperitoneally vaccinated with 1012 CFU fish−1 of formalin-killed S. iniae (V) or sham vaccinated (S) with sterile normal saline solution (NSS). Both groups were fed with Bacillus probiotic (5 × 109 CFU kg-1 diet) for 6 weeks. The protective efficacy of the vaccine against S. iniae examined at 4, 10 and 20 weeks post-vaccination showed that vaccinated (V) and vaccinated and probiotic (VP) groups had no …

The Impact Of Cow Behaviors During Transition Period On Reproductive And Milk Productive Performance, Aqeel Raza

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study investigates the impact of behavioral patterns during the transition and early postpartum periods on reproductive and lactation outcomes in tropical dairy cows, using the AfiTag-II biosensor to monitor activity, rest time, rest per bout, and restlessness ratio. Data from 298 prepartum and 347 postpartum Holstein Friesian cows, categorized by lactation number (1, 2, and ≥3), was analyzed. K-means clustering on 227 lactation records identified distinct behavioral groups, facilitating the assessment of reproductive traits through Cox regression and lactation curves using the Wood model. First-lactation cows had shorter prepartum rest durations (568.8 ± 5.4 min/day) compared to higher-parity cows, …

Genetic Characteristics Of Salmonella Carrying Plasmid-Associated Antimicrobial Resistance And Virulence Genes Isolated From Pigs, Pork, And Humans In The Bordering Provinces Between Thailand And Some Land Neighboring Countries, Siraphatson Wetchasirigul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Antimicrobial resistance (AMR) is a major public health threat. The issue has disproportionately affected in low- and middle-income countries including Thailand and its neighboring counties where high rates of resistant Salmonella have been continuously reported in pigs, pork, and humans. Recognizing the significant role of R plasmids in AMR distribution, knowledge on virulence genes associated plasmids is still limited. The frequency and patterns of virulence factors are still not routinely tracked. This study aimed to characterize Salmonella enterica isolates carrying plasmid-associated virulence and AMR genes from pig, pork, and human isolates in border provinces of Thailand, Lao PDR, Cambodia, and …

Evaluating The Efficacy Of An Early Vaccination Strategy After Farrowing Using A Modified Live Prrs Vaccine And Post-Weaning Challenge With Thai Hp-Prrs, Apichart Prapattanakullawong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Porcine reproductive and respiratory syndrome virus (PRRSV) is a major pathogen responsible for substantial economic losses in the global swine industry, adversely affecting both reproductive and respiratory health. In Thailand, early PRRSV infections in suckling and nursery pigs are commonly observed, necessitating the implementation of early vaccination strategies. However, early vaccination in young piglets has been controversial due to concerns about interfering maternal-derived antibodies (MDA) and the competency of the piglets' immune system. The objective of this study is to evaluate the efficacy of a modified-live virus (MLV) PRRS vaccination in early-age piglets from PRRSV-positive farms by assessing immune responses …

Genotypic Characterization And Amr Risk Assessment Of Esbl- Producing And Fluoroquinolone-Resistant Salmonella Spp. From Pork Collected In Bangkok, Thawanrut Kiatyingangsulee

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aimed to 1) determine the prevalence of ESBL-producing and fluoroquinolone-resistant Salmonella in pork, 2) investigate their genomic characteristics, and 3) estimate the risk of consumer exposure. Pork samples were collected from 58 markets across 19 districts in Bangkok. The study focused on third-generation cephalosporin resistance mediated by ESBLs and fluoroquinolone resistance, representing horizontal and vertical gene transfer, respectively. Out of 793 pork samples, 339 (42.8%) were positive for Salmonella, yielding 531 isolates from 33 serotypes. The most common were Salmonella Rissen (22.6%), Sinstorf (16.8%), and Derby (10.2%). Antimicrobial susceptibility testing showed 28.2% of isolates were ESBL producers and …

Efficacy Of Cell-Free Supernatants Based Nano-Application From Thai Probiotic Bacteria As An Alternative Preservative In Meat Against Foodborne Pathogens, Thin Zar Soe

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study evaluates whether the use of cell-free supernatants (CFS) derived from Thai probiotic bacteria as natural preservatives in meat products, provides a safer alternative to chemical preservatives. The objectives include developing a nano-encapsulation technique to enhance the stability and efficacy of CFS, and assessing the effectiveness of nano-encapsulated CFS (Nano-CFS) in maintaining meat safety and quality during storage and evaluating the antibacterial efficacy of CFS and (Nano-CFS) against common foodborne pathogens, determining the minimal inhibitory and bactericidal concentrations (MIC and MBC). The CFS was prepared from the Thai probiotic strains. Antibacterial activity was determined using agar well diffusion and …

Genomics Approach For Ex Situ Conservation Of Asian King Vulture And Steppe Eagle, Wannapol Buthasane

Chulalongkorn University Theses and Dissertations (Chula ETD)

The Asian king vulture (Sarcogyps calvus, AKV), an ecologically important forest scavenger, is an Old World vulture in the family Accipitridae. It is classified as a critically endangered species, with no genomic data currently available. This dissertation aimed to assemble a high-quality reference genome to enhance understanding of its genetic background, scavenging adaptations and genetic diversity within Thailand’s captive population. A hybrid genome assembly combining Pacific Biosciences (PacBio) long-read and MGI short-read sequencing was generated from DNA extracted from the whole blood of a female individual. Comparative genomic analyses with New World vultures (NWVs) and other Accipitridae species revealed distinct …

Ultrasonographic Renal Cortical Thickness To Abdominal Aorta Internal Diameterin Healthy Cats And Chronic Kidney Disease Cats, Run Sakulsirajit

Chulalongkorn University Theses and Dissertations (Chula ETD)

Chronic kidney disease (CKD) is a one of the most common disorder in cats, particularly in aging populations. Early detection and accurate monitoring are crucial for effective disease management. This study aims, first, to evaluate the ultrasonographic parameters, including renal cortical thickness (RCT), medullary thickness (MT), renal length (RL), abdominal aorta diameter (Ao) and ratios, including RCT/Ao, RCT/MT, RCT/RL, MT/Ao and RL/Ao compared between clinically healthy and CKD cats. Second, to evaluate the correlations of ultrasonographic parameters with blood renal functions including blood urea nitrogen (BUN), creatinine (Cr) and symmetric dimethylarginine (SDMA) and also correlation with age, body weight (BW) …

Improving Antimicrobial Susceptibility Testing Of Arcobacter Butzleri: Comparative Methods And Epidemiological Cut-Off Value (Ecoff) Determination, Myzatul Hanis Zahiyah Yusof

Chulalongkorn University Theses and Dissertations (Chula ETD)

Arcobacter butzleri is an emerging zoonotic pathogen associated with gastrointestinal illnesses in humans, yet standardized antimicrobial susceptibility testing (AST) methods and interpretive breakpoints for this microorganism remain unavailable. Broth microdilution, despite being endorsed by the CLSI-VAST subcommittee, is often impractical in several laboratories. Therefore, this study aimed to evaluate agar dilution as a more feasible alternative to broth microdilution. A total of 415 A. butzleri isolates from various meat sources in Bangkok, Thailand were confirmed by PCR and tested against ciprofloxacin, erythromycin, gentamicin, and tetracycline. AST was conducted using broth microdilution under aerobic conditions for 24 hours as the reference …