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Molecular Study Of The Prevalence Of Leptospira Spp. Serovar Hardjo In Blood Samples Of Iranian Cattle And Sheep, Faham Khamesipour, Abbas Doosti, Esmaeil Omrani
Molecular Study Of The Prevalence Of Leptospira Spp. Serovar Hardjo In Blood Samples Of Iranian Cattle And Sheep, Faham Khamesipour, Abbas Doosti, Esmaeil Omrani
The Thai Journal of Veterinary Medicine
Leptospira is an emerging global public health problem which involves both animals and human. The aim of this study was to appraise the presence of Leptospira DNA in blood samples from cattle and sheep via Polymerase Chain Reaction (PCR) method in Chaharmahal VaBakhtiari and Isfahan provinces of Iran. One hundred and ninety five samples (92 blood samples of cattle and 103 blood samples of sheep) were collected randomly from clinical healthy animals. DNA was extracted from the blood samples and stored at -20°C before examination. PCR reaction was performed for detection of Leptospira DNA using specific primers for 16s rRNA …