Veterinary Medicine Commons^™

Evaluation Of Wheat Blended With Corn In Finishing Diets Containing Wet Distillers Grains, C A. Coulson, B M. Boyd, B B. Conroy, Galen E. Erickson

Nebraska Beef Cattle Reports

An experiment was conducted to evaluate the effect of grain type and wet distillers grains inclusion on finishing cattle performance and carcass characteristics. It was hypothesized that a greater inclusion of wet distillers grains would help mitigate acidosis previously observed with feeding wheat. Treatments were designed as a 2 × 2 factorial arrangement, with the first factor as grain type at either 100% dry rolled corn or a 50:50 blend of dry- rolled wheat and dry rolled corn, and the second factor as wet distillers grains plus solubles (WDGS) inclusion at either 12 or 30% of diet dry matter. There …

Impact Of Feeding Aspergillus Subspecies Blend And Di! Erent Corn Processing Methods On Finishing Beef Cattle Performance And Carcass Characteristics, Stacia M. Hopfauf, Bradley M. Boyd, Levi Mcphillips, J. C. Macdonald, Galen E. Erickson

Nebraska Beef Cattle Reports

A feedlot study utilizing 320 crossbred calf- fed steers (initial body weight 588 lb) compared the effect of feeding an Aspergillus additive in either dry- rolled corn or high- moisture corn finishing diets on cattle performance and carcass characteristics. Steers were fed 0 g/steer daily or 10 g/steer daily Aspergillus for both corn processing methods. # ere were no significant interactions between corn processing method and Aspergillus. Feeding finishing cattle Aspergillus did not impact performance compared to feeding none. Cattle fed dry- rolled corn had greater final body weight, dry matter intake, and gain compared to high- moisture corn diets. …

Evaluation Of Processing Technique For High- Moisture And Dry Corn Fed To Finishing Cattle, C A. Coulson, B M. Boyd, Braden C. Troyer, Levi Mcphillips, Mitch M. Norman, Galen E. Erickson

Nebraska Beef Cattle Reports

A 134- day finishing trial was conducted to evaluate the effect of milling method and corn type on finishing cattle performance and carcass characteristics. Treatments were applied in a 2 × 3 factorial arrangement, with the first factor as milling method (Automatic Ag roller mill or hammer mill) and the second factor as corn type, either 100% dry corn, 50:50 blend of dry and high moisture corn, or 100% high moisture corn. $ ere was no interaction between milling method and corn type for carcass- adjusted final body weight, average daily gain, or dry matter intake but there was an …

Evaluating Finishing Performance Of Cattle Fed High- Moisture Corn And Steam- Flaked Corn Blends With Modified Distillers Grains, Braden C. Troyer, Zac C. Carlson, Levi Mcphillips, Andrea K. Watson, J. C. Macdonald, Galen E. Erickson

Nebraska Beef Cattle Reports

The objective of this study was to determine the impacts of feeding different inclusions of high- moisture corn or steam- flaked corn in diets with 20% modified distillers grains plus solubles. Additionally, this study was designed to quantify any associative effects when high- moisture and steam- flaked corn are blended together with modified distillers grains plus solubles. Cross-bred yearling steers (n=90; BW=777 ± 7.9 lb) were individually fed using a Calan Gate system for 168 days. Animals received one of five finishing diets containing 100% high moisture corn, one of three blends of high moisture steam- flaked, or 100% steam- …

Training Improves The Reliability Of Temperament Assessment In Cattle, Jamie T. Parham, Jessica J. Schmidt, Ronald M. Lewis

Nebraska Beef Cattle Reports

Accurate and precise measurement of docility in cattle is paramount when including temperament as a criterion for selection. The value of training individuals in assigning a docility score was evaluated by comparing the reliability of individual assessments of temperament in beef cattle before and after various instructional methods. Preceding training, participants’ assessment of cattle behavior, videoed while each heifer was restrained in a chute, was not impacted by age, gender, or pre- existing cattle handling experience. Groups of participants that received additional training were more accurate and precise in evaluating temperament, regardless of training method, compared to those without. No …

Mineral Concentrations Of Forages For Livestock In Nebraska And South Dakota, Rebecca J. Kern, John W. Kern, Hannah M.G. Dorn, Carrie E. Putnam, Janna J. Block, Adele A. Harty, M. E. Drewnoski

Nebraska Beef Cattle Reports

Forage samples from Nebraska and South Dakota submitted to Ward Laboratories, Inc. from 2012– 2019 were analyzed for mineral concentrations. Samples were categorized by forage species, quality based on protein content, and mineral concentration based on requirements for lactating beef cows. ! e data indicate that copper and zinc are frequently deficient across all species and levels of forage quality, emphasizing the need for supplementation. Except for magnesium, macro- mineral deficiencies are less likely to occur when feeding high quality forages in Nebraska and South Dakota. Corn feedstuffs are particularly likely to result in mineral deficiencies if fed without mineral …

Evaluation Of Models Used To Predict Dry Matter Intake In Forage- Based Diets, Aksel Wiseman, Andrea K. Watson, Rick Stock, Terry Klopfenstein

Nebraska Beef Cattle Reports

Accurately predicting intake is critical to model performance of cattle in order to formulate diets to meet nutritional requirements. Modeling systems must be accurate in order to provide correct information to producers. Multiple studies with growing cattle consuming forage- based diets were summarized. Actual gain and weights of the cattle were used to determine predicted dry matter intake using the Beef Cattle Nutrient Requirements Model (2016). ! e predicted dry matter intakes were compared to observed dry matter intakes to determine accuracy of the prediction model. ! e model over predicted intakes at low TDN and under predicted intakes at …

Growing Calf Intake Of Hay Or Crop Residue Based Diets, Aksel Wiseman, Andrea K. Watson, Rick Stock, Terry Klopfenstein

Nebraska Beef Cattle Reports

It is important to know or predict feed intake by growing calves on forage- based diets in order to balance these diets for nutrients such as energy and protein. Several growing calf studies with forage- based diets were summarized. ! ese studies evaluated the use of crop residue as a substitute for conventional forages, primarily grass hay. Calves gained about 1.8 lb/day for all forage- based diets. Calves consumed 2.6% of body weight daily when fed hay- based diets, but those fed residue with distillers grains diets consumed only 1.6% of body weight. However, when feeding the residue with distillers …

Impact Of Biochar Supplementation In Growing Diets On Greenhouse Gas Emissions, Jessica L. Sperber, Braden C. Troyer, Levi Mcphillips, Andrea K. Watson, Galen E. Erickson

Nebraska Beef Cattle Reports

A study was conducted to evaluate the impact of feeding biochar growing diets on cattle performance and methane and carbon dioxide emissions. Two treatments were evaluated, a forage- based control diet without biochar and a diet with biochar included at 0.8% of the diet dry matter, replacing ! ne ground corn in the supplement. Pens of cattle were rotated through a two- sided emissions barn (2 pens evaluated simultaneously) to capture CH4 and CO2 production. " ere were no statistical differences in performance or gas emissions for steers fed a biochar supplemented diet compared to control. Numerically, biochar supplemented steers …

Alternative Heifer Development Systems Utilizing Corn Residue And Cover Crops, Hannah F. Speer, Hannah E. Riley, R. A. Cushman, Harvey C. Freetly, M. E. Drewnoski

Nebraska Beef Cattle Reports

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Evaluation Of Biochar On Nutrient Loss From Fresh Cattle Manure, Jessica L. Sperber, Tyler Spore, Galen E. Erickson, Andrea K. Watson

Nebraska Beef Cattle Reports

An experiment was conducted to evaluate the impact of biochar and time on manure nutrient retention. Pans were used to simulate feedlot pens with 10 replications per treatment. Biochar was included at 0, 5, or 10% of manure dry matter with 30 and 60 day durations to evaluate pan contents over time. There was a 13- percentage unit increase in organic matter losses from day 30 to 60 for pans without biochar, and a 3- percentage unit increase for pans containing biochar. The least nitrogen loss was measured on the pans without biochar harvested at 30 days. Pans harvested at …

Impact Of Masters Choice Corn Silage On Nutrient Digestion In Growing Cattle, Jiehua Xiong, Mitch M. Norman, Hannah C. Wilson, Caleb Crabtree, Galen E. Erickson

Nebraska Beef Cattle Reports

A digestion study was conducted to evaluate Masters Choice corn silage hybrids on nutrient digestibility in growing beef steers. ! e three hybrids evaluated were a conventional hybrid (CON) commonly grown in Eastern Nebraska which served as the control, Masters Choice hybrid MCT6365 RIB (MC1) that has been selected to improve " ber and starch digestion and Masters Choice hybrid MCT6733 GT3000 (MC2) selected to improve " ber digestion. Treatment diets consisted of 80% of the diet dry matter (DM) of each corn silage hybrid. Steers fed MC1 corn silage had the greatest organic matter (OM), energy digestibility, and digestible …

Pseudomonas Survive Thermal Processing And Grow During Vacuum Packaged Storage In An Emulsified Beef System, Samuel C. Watson, Rebecca A. Furbeck, Byron D. Chaves, Gary A. Sullivan

Nebraska Beef Cattle Reports

New research has suggested the ability of Pseudomonas, a common spoilage microorganism, to grow in cooked beef products stored under vacuum which challenges the traditional understanding of the role of Pseudomonas during cooked beef spoilage. Understanding the mechanisms of survival and growth of Pseudomonas in these products is crucial for improving shelf life. The objective of this experiment was to determine Pseudomonas survival in a thermally processed, emulsified cooked beef model system. After eight weeks of refrigerated storage, Pseudomonas was recovered from cooked emulsified beef, indicating the potential for Pseudomonas to survive thermal processing and cause spoilage in cooked vacuum …

Dexamethasone Serum Concentrations After Intravenous Administration In Horses During Race Training, S. Mcclure, C. K. Fenger, Bradley Brown, George Maylin, W. Duer, L. Dirikolu, Kimberly Brewer, Jacob Machin, Thomas Tobin

Maxwell H. Gluck Equine Research Center Faculty Publications

Dexamethasone (DXM) sodium phosphate is a widely used corticosteroid for inflammatory conditions in horses, regulated in racing jurisdictions in the USA by a 0.005 ng/ml serum/plasma threshold. This study seeks to describe serum concentrations of DXM at 48 and 72 h after intravenous administration of 20 mg DXM sodium phosphate over 1 to 5 days, and to identify a possible source of DXM overages. 74 horses (39 Thoroughbreds, 13 Standardbreds, 22 Quarter Horses) in active race training received 20 mg DXM sodium phosphate. Serum was collected before injection, at 48 and 72 h post last injection, and analysed by LC/MS-MS …

Zilpaterol And Ractopamine: Reaching Consensus On Trace-Level Transfers To Racehorses, Jacob Machin, Kimberly Brewer, Clara K. Fenger, Sarah White-Springer, Abelardo Morales-Briceno, Thomas Tobin

Maxwell H. Gluck Equine Research Center Faculty Publications

In this article, we review the investigation of trace amounts of b-2 adrenergic agonists, a drug class that possess significant risk to the integrity of horse racing whether intentionally used or through the inadvertent contamination of feed.

Maturation Of Equine Proximal Sesamoid Bones In Thoroughbred Horses, Angela Maria Mangine

Theses and Dissertations--Veterinary Science

Proximal sesamoid bone (PSB) injuries account for approximately 50% of all fatal catastrophic musculoskeletal injuries in Thoroughbred racehorses. In addition, PSB fractures are not uncommon in Thoroughbred foals and yearlings as a result of energetic or intense free exercise. The ability to interpret some PSB pathology assessments, however, is more difficult given the limited information published on the normal development and maturation of these paired sesamoid bones. In addition, the level of normal variation in PSB morphological and structural parameters within the Thoroughbred population as a function of age, body size, gender, and inter-animal variation are not well documented, complicating …

Structural Adaptation Of Equine Proximal Sesamoid Bones To High-Speed Exercise, Kathryn Mary Babiarz

Theses and Dissertations--Veterinary Science

Proximal sesamoid bone (PSB) fractures are one of the most commonly reported catastrophic musculoskeletal injuries (CMI) in Thoroughbred racing. Despite the seriousness of these injuries, an understanding of the structural changes that occur normally within PSBs in response to the biomechanical forces of high-speed exercise and how they relate to pathological events leading to fracture remain limited. To address this critical knowledge gap, PSBs from Thoroughbred horses were analyzed with both computed tomography (CT) and microcomputed tomography (µCT) to assess their morphological and structural parameters. Comparisons were made using samples from the left and right forelimbs of Thoroughbreds of both …

An Assessment Of The Carrier State And A Novel Marker Of Leptospira And Abortion In Central Kentucky Horses, Gloria Louise Gellin

Theses and Dissertations--Veterinary Science

Leptospirosis is a reemerging zoonotic infection of worldwide importance and affects all mammals. The bacterium is transmitted to animals and humans by urine, fetal membranes and body fluids. Leptospira shedding in the urine contaminates both soil and water, exposing both humans and animals to the bacterium. Leptospirosis in horses can cause abortion and is one of the etiologies of equine recurrent uveitis which can lead to blindness. Equine leptospiral abortion in Central Kentucky is primarily caused by serovar Pomona, with occasional cases attributed to serovar Grippotyphosa. There are a few reports in the literature attributing abortion to serovar Bratislava in …

Muscle Mass And Immune Function In The Senior Horse, Alisa Christina Herbst

Theses and Dissertations--Veterinary Science

Senior horses (≥ 15 years) represent up to one-third of the global equine population, and the proportion of old horses (≥ 20 years) in the U.S. has been steadily increasing. Aging is associated with a loss of skeletal muscle mass in horses, and while age-related muscle loss is comparingly well characterized in humans, little is currently known concerning underlying mechanisms, adverse outcomes, or the prevalence of low muscle mass in senior horses. One factor proposed to play a role in the development of age-related muscle atrophy in humans is inflamm-aging, a low-grade inflammation that affects elderly people and that has …

Predicting Tomorrow: Optimizing The Early Detection Of Disease And Disease Recovery In Dairy Calves Using Precision Technologies, Melissa Cantor

Theses and Dissertations--Animal and Food Sciences

The leading causes of morbidity and mortality in preweaned dairy calves are diarrhea and bovine respiratory disease complex (BRD). The delayed detection of these diseases in calves can also delay intervention and disease recovery. The overarching objective of this dissertation was to follow a cohort of calves daily for the first 90 days of life for naturally occurring clinical BRD bouts and diarrheal bouts. The objective answered if feeding behaviors and activity levels were different in at-risk calves during BRD development and BRD recovery from an antimicrobial intervention. Furthermore, the potential of colostrum replacer as a feeding intervention strategy to …

Science-Based Regulation Of Pharmacological Substances In Competition Horses, Jacob Machin

Theses and Dissertations--Toxicology and Cancer Biology

Current testing methodologies within equine forensic toxicology focus on arbitrary thresholds and zero-tolerance policy. Modern analytical chemistry’s limits of detection are low enough that oftentimes femtogram-per-milliliter amounts of a substance can readily be identified in both blood and urine of a horse. For most pharmacologically relevant compounds, these concentrations have no relevance to pharmacological effect. It is therefore crucial that testing methodologies to determine appropriate thresholds and cut-offs be developed that are driven by biological activity rather than arbitrary limits of detection. This dissertation looks to address this by suggesting a system of calculated Effective Plasma Concentrations by which a …

Cellular Proliferation Of Equine Bone Marrow- And Adipose Tissue-Derived Mesenchymal Stem Cells Decline With Increasing Donor Age, Jasmin Bagge, James N. Macleod, Lise C. Berg

Maxwell H. Gluck Equine Research Center Faculty Publications

Background: Bone marrow (BM)- and adipose tissue (AT)-derived mesenchymal stem cells (MSCs) are used increasingly for autologous cell therapy in equine practice to treat musculoskeletal and other injuries. Current recommendations often call for 10–100 million MSCs per treatment, necessitating the expansion of primary cells in culture prior to therapeutic use. Of concern, human and rodent studies have shown a decline of both MSC recovery from sampled tissue and in vitro proliferative capacity with increasing donor age. This may be problematic for applications of autologous cell-based therapies in the important equine demographic of older patients.

Objectives: To investigate the effect of …

Climate Change Is Likely To Increase The Development Rate Of Anthelmintic Resistance In Equine Cyathostomins In New Zealand, Christian W. Sauermann, Dave M. Leathwick, Mark Lieffering, Martin K. Nielsen

Maxwell H. Gluck Equine Research Center Faculty Publications

Climate change is likely to influence livestock production by increasing the prevalence of diseases, including parasites. The traditional practice of controlling nematodes in livestock by the application of anthelmintics is, however, increasingly compromised by the development of resistance to these drugs in parasite populations. This study used a previously developed simulation model of the entire equine cyathostomin lifecycle to investigate the effect a changing climate would have on the development of anthelmintic resistance. Climate data from six General Circulation Models based on four different Representative Concentration Pathways was available for three New Zealand locations. These projections were used to estimate …

The Effects Of Music On Dairy Production, Anneliese Kemp

Honors College Theses

The purpose of this research is to find which genre of music dairy cows produce the most milk to in order to aid dairy farmers in yielding maximum product while also keeping their cattle as comfortable as possible. During each lactation a different genre of music will play, basic vital signs, and behavioral observations of the cows will be taken, then the amount of milk produced during that genre will be measured. First, no music will be played, base line vitals will be taken, and milk yield will be measured. This will give an estimate to how positively or negatively …

The Surveillance Of Gastrointestinal Parasitic Nematodes Of Northwest Arkansas Dairy Cattle Using Traditional And Genetic Parasitological Identification Procedures, Eva M. Wray

Graduate Theses and Dissertations

Traditional and genetic parasitological identification procedures were compared using naturaland artificial nematode parasite infections in Holstein steer calves. The traditional parasitological procedures measured fecal egg counts, coprocultures with subsequent larval collection and adult nematodes collected at necropsy. The genetic identification procedures measured ITS-2 sequences extracted from different stages of nematode development: raw feces, concentrated nematode eggs, third stage larvae and adults. The primary nematodes observed were Cooperia oncophora, Cooperia punctata and Ostertagia ostertagi. The traditional techniques were not significantly different from one another, while the genetic sequencing showed variation amongst the different procedures. The raw feces sequences showed the most …

Comparative Analysis Of Intestinal Helminth Infections In Colic And Non-Colic Control Equine Patients, Heidrun Gehlen, Nadine Wulke, Antonia Ertelt, Martin K. Nielsen, Simone Morelli, Donato Traversa, Roswitha Merle, Douglas Wilson, Georg Von Samson-Himmelstjerna

Maxwell H. Gluck Equine Research Center Faculty Publications

All around the world, intestinal helminths constitute one of the most prevalent life-long occurring infections and re-infections affecting all horse age groups. A range of parasite species among strongyles, ascarids, and tapeworms is known to have the potential to cause colic in horses. However, there is a lack of current scientific evidence on the actual relevance of helminth infection levels in the context of colic in horses kept during prevailing epidemiological conditions. Thus, a prospective case-control study on the occurrence of intestinal helminths in a total of 620 mainly adult equine clinic patients was conducted to investigate the association between …

Meta-Analysis Of Cyathostomin Species-Specific Prevalence And Relative Abundance In Domestic Horses From 1975-2020: Emphasis On Geographical Region And Specimen Collection Method, Jennifer L. Bellaw, Martin K. Nielsen

Maxwell H. Gluck Equine Research Center Faculty Publications

BACKGROUND: Cyathostomins infect virtually all horses, and concomitant infections with 10 or more species per horse is standard. Species-specific knowledge is limited, despite potential species bias in development of disease and anthelmintic resistance. This is the first meta-analysis to examine effects of geographical region and cyathostomin collection method on reported composition of cyathostomin communities.

METHODS: Thirty-seven articles published in English in 1975 or later, in which adults of individual species were systematically enumerated, were included. Seven regions; North America, South America, eastern Europe, western Europe, northern Europe, southern Africa, and Oceania, and three cyathostomin collection methods; (i) standard necropsy recovery …

Importation Of Macrocyclic Lactone Resistant Cyathostomins On A Us Thoroughbred Farm, Martin K. Nielsen, M. Banahan, R. M. Kaplan

Maxwell H. Gluck Equine Research Center Faculty Publications

Anthelmintic resistance in equine cyathostomins is both widespread and highly prevalent in the benzimidazole and tetrahydropyrimidine classes; however, reports of resistance to macrocyclic lactone (ML) drugs are sparse and sporadic. This study reports a case of clear ML resistance in a group of Thoroughbred yearlings imported from Ireland to the US in 2019. Fecal egg count reduction (FECR) following ivermectin administered in February 2020 demonstrated 100% reduction in the US bred yearlings, but 93.5%, 70.5%, and 74.5% reduction in three groups of the imported yearlings. The two former groups were then retreated with ivermectin, yielding FECRs of 33.8% and 23.5%, …

The P-Glycoprotein Repertoire Of The Equine Parasitic Nematode Parascaris Univalens, Alexander P. Gerhard, Jürgen Krücken, Emanuel Heitlinger, I. Jana I. Janssen, Marta Basiaga, Sławomir Kornaś, Céline Beier, Martin K. Nielsen, Richard E. Davis, Jianbin Wang, Georg Von Samson-Himmelstjerna

Maxwell H. Gluck Equine Research Center Faculty Publications

P-glycoproteins (Pgp) have been proposed as contributors to the widespread macrocyclic lactone (ML) resistance in several nematode species including a major pathogen of foals, Parascaris univalens. Using new and available RNA-seq data, ten different genomic loci encoding Pgps were identified and characterized by transcriptome-guided RT-PCRs and Sanger sequencing. Phylogenetic analysis revealed an ascarid-specific Pgp lineage, Pgp-18, as well as two paralogues of Pgp-11 and Pgp-16. Comparative gene expression analyses in P. univalens and Caenorhabditis elegans show that the intestine is the major site of expression but individual gene expression patterns were not conserved between the two nematodes. In P. …

Metagenomic, Viral And Host Genetic Analyses Of Congenital Tremor In Pigs, Kylee Sutton

School of Biological Sciences: Dissertations, Theses, and Student Research

The Pestivirus genus contains several viral species having a major impact on the livestock species. While there were only four major pestiviral species for a time, recent metagenomic sequencing approaches identified additional species, such as atypical porcine pestivirus (APPV). Congenital tremor was first identified almost a hundred years ago and since still has an impact on swine health. There are two main types of congenital tremor, type A and B, with type A congenital tremor further subcategorized based on causative agent. Until recently, type A-II congenital tremor did not have a known cause. Recent viral sequencing of affected samples, including …