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Translational Medical Research Commons™
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Articles 1 - 3 of 3
Full-Text Articles in Translational Medical Research
Transgenic Mice Expressing Lipoprotein Lipase In Adipose Tissue: Absence Of The Proximal 3′-Untranslated Region Causes Translational Upregulation, Lori L. Hensley, Gouri Ranganathan, Elke M. Wagner, Brian D. Wells, Joseph C. Daniel, Diane Vu, Clay F. Semenkovich, Rudolf Zechner, Philip A. Kern
Transgenic Mice Expressing Lipoprotein Lipase In Adipose Tissue: Absence Of The Proximal 3′-Untranslated Region Causes Translational Upregulation, Lori L. Hensley, Gouri Ranganathan, Elke M. Wagner, Brian D. Wells, Joseph C. Daniel, Diane Vu, Clay F. Semenkovich, Rudolf Zechner, Philip A. Kern
Clinical and Translational Science Faculty Publications
Lipoprotein lipase (LPL) is a key enzyme in lipoprotein and adipocyte metabolism. Defects in LPL can lead to hypertriglyceridemia and the subsequent development of atherosclerosis. The mechanisms of regulation of this enzyme are complex and may occur at multiple levels of gene expression. Because the 3′-untranslated region (UTR) is involved in LPL translational regulation, transgenic mice were generated with adipose tissue expression of an LPL construct either with or without the proximal 3′-UTR and driven by the aP2 promoter. Both transgenic mouse colonies were viable and expressed the transgene, resulting in a 2-fold increase in LPL activity in white adipose …
Adiponectin Expression From Human Adipose Tissue: Relation To Obesity, Insulin Resistance, And Tumor Necrosis Factor-Α Expression, Philip A. Kern, Gina B. Di Gregorio, Tong Lu, Negah Rassouli, Gouri Ranganathan
Adiponectin Expression From Human Adipose Tissue: Relation To Obesity, Insulin Resistance, And Tumor Necrosis Factor-Α Expression, Philip A. Kern, Gina B. Di Gregorio, Tong Lu, Negah Rassouli, Gouri Ranganathan
Clinical and Translational Science Faculty Publications
Adiponectin is a 29-kDa adipocyte protein that has been linked to the insulin resistance of obesity and lipodystrophy. To better understand the regulation of adiponectin expression, we measured plasma adiponectin and adipose tissue adiponectin mRNA levels in nondiabetic subjects with varying degrees of obesity and insulin resistance. Plasma adiponectin and adiponectin mRNA levels were highly correlated with each other (r = 0.80, P < 0.001), and obese subjects expressed significantly lower levels of adiponectin. However, a significant sex difference in adiponectin expression was observed, especially in relatively lean subjects. When men and women with a BMI2were compared, women had a twofold higher percent body fat, yet their plasma adiponectin levels were 65% higher (8.6 ± 1.1 and 14.2 ± 1.6 μg/ml in men and women, respectively; P < 0.02). Plasma adiponectin had a strong association with insulin sensitivity index (SI) (r = 0.67, P < 0.0001, n = 51) that was not affected by sex, but no relation with insulin secretion. To separate the effects of obesity (BMI) from SI, subjects who were discordant for SI were matched for BMI, age, and sex. Using this approach, insulin-sensitive subjects demonstrated a twofold higher plasma level of …
Research Annual Report 2002, Children's Mercy Hospital
Research Annual Report 2002, Children's Mercy Hospital
Research Annual Reports
Annual report of the research at Children's Mercy Hospital in Kansas City MO, a pediatric medical center.