Pharmacy and Pharmaceutical Sciences Commons^™

Transcriptomic Changes And The Roles Of Cannabinoid Receptors And Pparγin Developmental Toxicities Following Exposure To Δ9-Tetrahydrocannabinol And Cannabidiol, Zacharias Pandelides, Neelakanteswar Aluru, Cammi Thornton, Haley E. Watts

Faculty and Student Publications

Human consumption of cannabinoid-containing products during early life or pregnancy is rising. However, information about the molecular mechanisms involved in early life stage Δ9-tetrahydrocannabinol (THC) and cannabidiol (CBD) toxicities is critically lacking. Here, larval zebrafish (Danio rerio) were used to measure THC- and CBD-mediated changes on transcriptome and the roles of cannabinoid receptors (Cnr) 1 and 2 and peroxisome proliferator activator receptor γ(PPARγ) in developmental toxicities. Transcriptomic profiling of 96-h postfertilization (hpf) cnr+/+ embryos exposed (6 - 96 hpf) to 4 μM THC or 0.5 μM CBD showed differential expression of 904 and 1095 genes for THC and CBD, respectively, …

The Role Of Cannabinoid Receptor 2 In Zebrafish Toxicities Following Developmental Exposure To Thc Or Cbd, Haley Watts

Honors Theses

As the laws concerning the regulation of Cannabis Sativa change across the globe, maternal use of cannabinoid-containing products during pregnancy is a greater concern than ever before. Little research has been conducted on the consequences of maternal use and developmental exposure to ∆9-tetrahydrocannabinol (THC) and cannabidiol (CBD. In this study, larval zebrafish (Danio rerio) were used to measure CBD and THC-mediated adverse outcomes including effects on larval length, eye area, and behavior in the cnr^+/+ and cnr2^-/- zebrafish strains where we discovered that cannabinoid exposure results in a decrease in each of these endpoints. Adult …

Impacts Of Embryonic Exposure To Cannabidiol Or ∆9-Tetrahydrocannabinol On Zebrafish (Danio Rerio) Frailty In F0 And F1 Generations, Anika Faruque

Honors Theses

Exposure to cannabinoids during critical development periods has increased with epileptic children being commonly prescribed CBD for seizures and pregnant women taking it recreationally. Many studies have been done on the possible benefits and drawbacks of cannabinoid exposure on the human brain, but not much is known about how it can affect the developing brain long-term. To see the potential adverse effects of cannabinoid exposure during critical stages of development and discover potential developmental origins of disease in consuming cannabinoids during embryogenesis, zebrafish embryos (6-96 hours post fertilization) were exposed to varying concentrations of CBD (0.02, 0.1, 0.5 µM), THC …

Biopharmaceutical Revolution In Saudi Arabia: Progress And Development, Rihana Alzahran, Elaine Harris

Articles

Purpose This paper aimed to analyze the situation of the biopharmaceutical industry in Saudi Arabia to improve the situation in a way that achieves high levels of development similar to those in Europe, the United States of America (USA), and Japan. Methods To achieve the objective of the study, the authors surveyed 18 out of 21 Saudi pharmaceutical companies that have received licensing for manufacturing from the Saudi Food and Drug Authority (SFDA) to diagnose the situation of manufacturing in relation to biopharmaceuticals. An interview with SFDA officials was carried out as a supplement to the survey. Results Findings revealed …

High-Throughput Sequencing-Based Approaches For The Identification And Development Of Molecular Targets For Cancer Therapy, David Joel Oliver

Theses and Dissertations

Effective cancer treatment depends on the development of therapeutics that efficiently eliminate the disease without significant reduction to quality of life. Targeted therapeutics achieve these goals, but their long-term efficacy is limited by the ability of cancers to become resistant to these therapies over time. The identification of new therapeutic targets is crucial for the future of cancer treatment. In this work we present functional identification and characterization of novel targets for cancer therapy.

Loss-of-function screens are a well-founded approach for the functional identification of new therapeutic targets, and, in combination with high-throughput sequencing technology, can now be performed at …

Turning Packaging Into Profit, Rihaz Z. Chughatta

Rihaz Z Chughatta

Packaging can be an expensive function to maintain, but it doesn’t have to be. In fact, Packaging can be a source of income for a company. More accurately, what if you took a look at your company’s Profit and Loss and saw Packaging as a negative expense? That’s right, you can turn packaging into profit for your company. How do you accomplish this feat? Glad you asked … read on to find three simple ways to start this process (and satisfy your curiosity) …

Outsourcing The Packaging Function, Rihaz Z. Chughatta

Rihaz Z Chughatta

If you are currently working in the packaging department of a major corporation in the pharmaceutical, food or consumer products industry, you have probably been exposed to some form of outsourcing, which is a global trend that has emerged over the past decade, and continues to evolve, within the packaging field.