Pharmacy and Pharmaceutical Sciences Commons^™

Paroxetine Is Pro-Inflammatory In Human Brain Microvascular Endothelial Cells, Syeda Hashimi, Sulay Patel, Omar Hassan, Bukola Odeniyi

Graduate Research Posters

BACKGROUND: Paroxetine is an antidepressant that is widely used. However, Paroxetine possesses anticholinergic properties which may contribute to cognitive decline in older adults. Surprisingly, the mechanisms of the cognitive decline of anticholinergic drugs in older adults remains elusive. A hypothesis is that a loss of cholinergic signaling is pro-inflammatory in the brain. We designed experiments to determine if Paroxetine is pro-inflammatory in Normal Human Brain Microvascular Endothelial Cells (NHBMECs).

METHOD: NHBMECs were grown at 5% CO2, 37C and 95% relative humidity until 90% confluent. NHBMECs were treated with the RNA free water as control, Paroxetine as 1.67x 10 4 mM/mol, …

Dendrimer Conjugation Enhances Tumor Penetration And Cell Kill Of Doxorubicin In 3d Coculture Lung Cancer Models, Rashed Almuqbil, Rodrigo S. Heyder, Elizabeth R. Bielski, Mikhail Durymanov, Joshua J. Reineke, Sandro R. P. Da Rocha

Graduate Research Posters

Background: Doxorubicin (DOX) is a potent chemotherapeutic widely used for solid tumors (1). Despite high efficacy in 2D cell culture, DOX efficacy does not translate to in vivo lung cancer models (2). Major side effects such as cardiotoxicity may be alleviated with nano-based drug delivery systems (nanoDDS). However, tumor penetration of DOX and DOX-nanoDDS is largely unknown and is an additional barrier to effective clinical therapy (3). Here we describe a nanoDDS capable of enhancing the penetration of DOX.

Methods: DOX was conjugated to generation 4 poly(amido-amine) dendrimers through (GFLG) tumor- liable bond. G4SA-GFLG-DOX was synthesized/characterized. spheroids were formed of …

A Pre-Transplant Blood-Based Lipid Signature For Prediction Of Antibody-Mediated Rejection In Kidney Transplant Patients, Monther Al Sultan, Daniel Contaifer Jr., Joshua Morriss, Suad Alshammari, Jeffrey Stern, Sindhura Bobba, Pamela Kimball, Anne King, Dhiren Kumar, Marlon Levy, Gaurav Gupta, Dayanjan Wijesinghe

Graduate Research Posters

Purpose. The aim of this study is to demonstrate the potential of the pre-transplant lipidome to predict post-transplant antibody-mediated rejection (AMR) in kidney transplant patients.

Methods. Patients were selected from a prospective observational cohort of a single-center adult kidney transplant center in the United States. The study included 16 kidney transplant patients who develop AMR within 2 years post-transplant and 29 stable control (SC) kidney transplant patients who did not develop AMR at any time within the post-transplant follow up. Selection of group differences on the day of transplant was determined by t-test analysis. Stepwise forward method was used to …

Afm Probing Of Amyloid-Beta 42 Dimers And Trimers, Sibaprasad Maity, Yuri L. Lyubchenko

Journal Articles: Pharmaceutical Sciences

Elucidating the molecular mechanisms in the development of such a devastating neurodegenerative disorder as Alzheimer's disease (AD) is currently one of the major challenges of molecular medicine. Evidence strongly suggests that the development of AD is due to the accumulation of amyloid β (Aβ) oligomers; therefore, understanding the molecular mechanisms defining the conversion of physiologically important monomers of Aβ proteins into neurotoxic oligomeric species is the key for the development of treatments and preventions of AD. However, these oligomers are unstable and unavailable for structural, physical, and chemical studies. We have recently developed a novel flexible nano array (FNA)-oligomer scaffold …

Exogenous Flupirtine As Potential Treatment For Cln3 Disease, Katia Maalouf, Joelle Makoukji, Sara Saab, Nadine J. Makhoul, Angelica V. Carmona, Nihar Kinarivala, Noël Ghanem, Paul C. Trippier, Rose-Mary Boustany

Journal Articles: Pharmaceutical Sciences

CLN3 disease is a fatal neurodegenerative disorder affecting children. Hallmarks include brain atrophy, accelerated neuronal apoptosis, and ceramide elevation. Treatment regimens are supportive, highlighting the importance of novel, disease-modifying drugs. Flupirtine and its new allyl carbamate derivative (compound 6) confer neuroprotective effects in CLN3-deficient cells. This study lays the groundwork for investigating beneficial effects in Cln3^Δex7/8 mice. WT/Cln3^Δex7/8 mice received flupirtine/compound 6/vehicle for 14 weeks. Short-term effect of flupirtine or compound 6 was tested using a battery of behavioral testing. For flupirtine, gene expression profiles, astrogliosis, and neuronal cell counts were determined. Flupirtine improved neurobehavioral parameters in …

Exploring Healthcare Services Utilization And Medication Adherence Of The Arab Descent Americans In South Florida, Haifa Abdulrahman Fadil

Student Theses, Dissertations and Capstones

Research into health outcomes of Arab-descent Americans (ADAs) is sparse. ADAs identify as “White,” instead of in a separate census category, contributing to their underrepresentation in the census and the limited amount of research on their health outcomes. In the current U.S. sociopolitical climate, better understandings are needed of the impact of discrimination and Arabic culture, including level of health literacy, on ADAs’ general health and health outcomes. This study employed mixed methods to explore the impact of health literacy level and perceived discrimination on HSU and medication adherence for ADAs in South Florida. In its qualitative phase, a convenience …

Chronic Liver Disease, Medha Singhal, Rajendra Prasad, Suresh Babu, Manthappa M

Digital Journal of Clinical Medicine

No abstract provided.

A Case Of Tetanus With Classical Presentations, Maaz Khan, Dr. Bhanukumar M

Digital Journal of Clinical Medicine

No abstract provided.

Airway Management In Case Of Granulomatosis With Polyangiitis, Dr. Pranay Penmetsa, Dr. Subramanian R

Digital Journal of Clinical Medicine

No abstract provided.

Multiple Sclerosis With Depression, Dr. Siri Gangadkar, Dr. Megha Tandure, Dr. Kishor M

Digital Journal of Clinical Medicine

No abstract provided.

Suicide Attempt In Relation To Covid-19 Crisis, Dr. Sreelakshmi Rajeev, Dr. Pranahith Reddy, Ms. Chinmayi K., Dr. Kishor M

Digital Journal of Clinical Medicine

No abstract provided.

A Rare Cause Of Hyponatremia, Dr. Tamanna Gupta, Dr. Pratibha Pereira

Digital Journal of Clinical Medicine

No abstract provided.

A Case Of Pancreatic Pseudocyst Following Acute Pancreatitis, Dr Hitha Suresh, Dr Madhu C P

Digital Journal of Clinical Medicine

No abstract provided.

Collagen Xii Is A Regulator Of Corneal Stroma Structure And Function, Mei Sun, Nabeel Zafrullah, Floriane Devaux, Chinda Hemmavanh, Sheila Adams, Noel M. Ziebarth, Manuel Koch, David E. Birk, Edgar M. Espana

Molecular Pharmacology & Physiology Faculty Publications

Purpose: The aim of this study was to determine the roles of collagen XII in the regulation of stromal hierarchical organization, keratocyte organization, and corneal mechanics.

Methods: The temporal and spatial expression of collagen XII at postnatal days 4, 10, 30, 90, and 150 were evaluated in wild-type (WT) mice. The role of collagen XII in hierarchical organization was analyzed by measuring fibril diameter and density, as well as stromal lamellar structure, within ultrastructural micrographs obtained from WT and collagen XII-deficient mice (Col12a1–/–). Keratocyte morphology and networks were assessed using actin staining with phalloidin and in vivo confocal …

Effects Of An Exogenous Ketone Supplement On Five‐Kilometer Running Performance, Philip J. Prins, Andrew P. Koutnik, Dominic P. D’Agostino, Christopher Q. Rogers, Jacob F. Seibert, Jillian A. Breckenridge, Daniel S. Jackson, Edward J. Ryan, Jeffrey D. Buxton, Dana L. Ault

Molecular Pharmacology & Physiology Faculty Publications

Numerous oral ketone supplements are marketed with the claim that they will rapidly induce ketosis and improve exercise performance. The purpose of this study was to assess exercise performance time and related physiological, metabolic and perceptual responses of recreational endurance runners after ingestion of a commercially available oral ketone supplement. Recreational endurance runners (n = 10; age: 20.8 ± 1.0 years; body mass: 68.9 ± 5.6 kg; height: 175.6 ± 4.9 cm) participated in a double-blind, crossover, repeated-measures study where they were randomized to 300 mg.kg-1 body weight of an oral β-hydroxybutyrate-salt + Medium Chain Triglyceride (βHB-salt+MCT) ketone supplement or …

Ketone Bodies Attenuate Wasting In Models Of Atrophy, Andrew P. Koutnik, Angela M. Poff, Nathan P. Ward, Janine M. Deblasi, Maricel A. Soliven, Matthew A. Romero, Paul A. Roberson, Carl D. Fox, Michael D. Roberts, Dominic P. D'Agostino

Molecular Pharmacology & Physiology Faculty Publications

Background: Cancer Anorexia Cachexia Syndrome (CACS) is a distinct atrophy disease negatively influencing multiple aspects of clinical care and patient quality of life. Although it directly causes 20% of all cancer-related deaths, there are currently no model systems that encompass the entire multifaceted syndrome, nor are there any effective therapeutic treatments.

Methods: A novel model of systemic metastasis was evaluated for the comprehensive CACS (metastasis, skeletal muscle and adipose tissue wasting, inflammation, anorexia, anemia, elevated protein breakdown, hypoalbuminemia, and metabolic derangement) in both males and females. Ex vivo skeletal muscle analysis was utilized to determine ubiquitin proteasome degradation pathway activation. …

Anticancer Activity Of Cepharanthine On Non-Small Cell Lung Cancer Cells, Yaowaluck Detpichai

Chulalongkorn University Theses and Dissertations (Chula ETD)

Cepharanthine (CEP) is a biscoclaurine alkaloid, isolated from the roots of Stephania cepharanthine Hayata that possesses potent anticancer properties against several types of cancer such as hepatocellular carcinoma, colorectal cancer, leukemia and ovarian cancer. However, there is still limited information regarding to its effects on non-small cell lung cancer cells. Therefore, the objectives of the present study were to determine the cytotoxicity of CEP and its underlying mechanisms in non-small cell lung cancer cells expressing wild-type p53 and EGFR (A549) and non-small cell lung cancer cells expressing mutant p53 and EGFR (H1975). The results indicated that CEP significantly inhibited the …

Profiling Transcription And Retrotransposition Of Mouse L1 Subfamilies, Lingqi Kong

Electronic Theses and Dissertations

Transposable elements, also called jumping genes, comprise almost 45% of the human genome. In contrast, only 1% of the human genome is protein-coding sequences. The function and advantages of maintaining such massive copies of transposable elements in the human genome are still unclear. Long interspersed element 1 (L1), the most substantial group and the only active autonomous transposable element in the human genome, has revealed its unique roles in many diseases. The insertional mutagenesis induced by L1 retrotransposition events could threaten human genomic stability and generate unexpected mutations. L1 overexpression has been documented in both somatic and germline cells and, …

Anaphylaxis Diagnosis And Management In The Emergency Department Of A Tertiary Hospital In The Philippines, Michelle Joy De Vera, Iris Conela Tagaro

Ateneo School of Medicine and Public Health Publications

Background:
In the Emergency Department (ED), diagnosis and management of anaphylaxis are challenging with at least 50% of anaphylaxis episodes misdiagnosed when the diagnostic criteria of current guidelines are not used.

Objective:
Objective of our study was to assess anaphylaxis diagnosis and management in patients presenting to the ED.

Methods:
Retrospective chart review conducted on patients presenting to The Medical City Hospital ED, the Philippines from 2013–2015 was done. Cases were identified based on International Statistical Classification of Diseases, 10th revision coding for either anaphylaxis or other allergic related diagnosis. Cases fitting the definition of anaphylaxis as identified by the …

Chemosensitizing Effect Of Streptomyces Crude Extract On Cisplatin-Induced Apoptosis In Human Lung Cancer H460 Cells, Theint Myat Noe Paing

Chulalongkorn University Theses and Dissertations (Chula ETD)

Although cisplatin has been considered as a first-line chemotherapeutic drug for the treatment strategy of lung cancer, its curative efficacy is limited by the occurrence of drug resistance and adverse effects. The application of non-toxic natural products for sensitization of cancer cells to conventional drugs is a recent novel strategy for cancer management to tackle these problems. This study aims to investigate effective chemosensitizer produced by Streptomyces sp. on the growth inhibition of human H460 lung cancer cells. Herein, three of eight strains isolated from peat swamp forest, Yala Province, Thailand which showed good purity analyzed by 16S rRNA sequencing …

Ndrg1 And Myelin-Related Disease: Alcoholism And Chemotherapy-Induced Neuropathy, Guy Harris

Theses and Dissertations

Alcohol use disorder (AUD) is a prevalent neuropsychiatric disease with profound health, social, and economic consequences. With an estimated 50% heritability, identifying genes that engender risk and contribute to the underlying neurobiological mechanisms represents an important first step in developing effective treatments. Gene expression studies are an important source of candidate genes for studying AUD, providing windows into the molecular machinery engaged by the brain in response to ethanol. Our laboratory has implicated N-myc down-regulated gene 1 (Ndrg1) as a potential candidate gene that modulates ethanol-induced changes in myelin-related gene expression and acute sensitivity to ethanol. Analysis of …

Anticholinergic Burden And Risk Of Cognitive Impairment In Older Adults, Syeda Hashimi

Theses and Dissertations

Studies reveal that 10-27% of older adults chronically use anticholinergic medications. Increased cumulative anticholinergic burden scores are associated with increased risks of dementia. The mechanisms by which anticholinergic drugs negatively impact cognition remain elusive. However, researchers speculate that the drug’s impairment of cholinergic neurons promotes neuroinflammation.

We hypothesize that drugs with anticholinergic properties will induce inflammation in the brain. MCP1 and IL 6 are chemokines that contribute to neuroinflammation. We investigated the influence of diphenhydramine (Benadryl) on the production of MCP1 and IL-6 in Normal Human Astrocytes and Paroxetine on the production of MCP1 in Normal Human Astrocytes and Normal …

Evaluating Appropriateness And Diagnostic Stewardship Opportunities Of Multiplex Polymerase Chain Reaction Gastrointestinal Testing Within A Hospital System, Melissa O'Neal, Hanna Murray, Sangita Dash, Majdi N. Al-Hasan, Julie Ann Justo, P Brandon Bookstaver

Faculty Publications

Objective: This single-center, retrospective, observational cohort study evaluates the appropriateness of the BioFire® FilmArray® Gastrointestinal (GI) multiplex PCR panel testing at a community-teaching hospital. Methods: All adult, hospitalized patients at Prisma Health Richland Hospital with a documented GI multiplex PCR panel from 1 April 2015 through 28 February 2018 were included in the analysis. Inappropriate use of the GI panel was defined as a test obtained without documented diarrhea, greater than 2 days of hospitalization, redundant use with other diagnostic tests (e.g. PCR), or laxative use in the preceding 48 h. Antibiotic use and host variables were compared between groups …

Online Availability Of Fish Antibiotics And Documented Intent For Self-Medication, Weiwei Zhang, Austin Williams, Nicole Griffith, Jessica Gaskins, P Brandon Bookstaver

Faculty Publications

Self-medication and antibiotic utilization without healthcare oversight may lead to delayed appropriate treatment, transmission of communicable infections, untoward adverse events, and contribute to antimicrobial resistance. Previous data suggest people obtain over-the-counter (OTC) animal antibiotics for their personal use. This study examined the availability of OTC fish antibiotics online and the documented intent for self-medication. The authors conducted a web-based cross-sectional study using Google search engine to identify vendor websites selling fish antibiotics in the United States. Vendor websites were included if product information, consumer reviews, and comments were publicly available. Nine fish antibiotics were chosen due to their possibility of …

Healthcare Practitioners’ Views Of Social Media As An Educational Resource, Adam G. Pizzuti, Karan H. Patel, Erin K. Mccreary, Emily Heil, Christopher M. Bland, Eric Chinaeke, Bryan L. Love, P Brandon Bookstaver

Faculty Publications

Social media is increasingly utilized as a resource in healthcare. We sought to identify perceptions of using social media as an educational tool among healthcare practitioners. An electronic survey was distributed to healthcare administrators, nurses, nurse practitioners, pharmacists, physicians, and physician assistants f hospital systems and affiliated health science schools in Georgia, Maryland, South Carolina, and Wisconsin. Survey questions evaluated respondents' use and views of social media for educational purposes and workplace accessibility using a Likert scale (1 = strongly disagree, 5 = strongly agree). Nurses (75%), pharmacists (11%), and administrators (7%) were the most frequent respondents. Facebook® (27%), Pinterest® …

Interleukin 1Β And Prostaglandin E2 Affect Expression Of Dna Methylating And Demethylating Enzymes In Human Gingival Fibroblasts, Sara Seutter, Jacquay Winfield, Alexis Esbitt, Samantha Snyder, Anastasia Magner, Kristine Kim, Craig Carcuffe, Jeremy Schmoyer, Payvand Kamrani, Jason Mercando, Seyed M Shamseddin, Kevan Green, Ruth C. Borghaei

PCOM Scholarly Works

Periodontitis is a common chronic inflammatory condition that results in increased levels of inflammatory cytokines and inflammatory mediators. In addition to oral disease and tooth loss, it also causes low-grade systemic inflammation that contributes to development of systemic conditions including cardiovascular disease, pre-term birth, diabetes and cancer. Chronic inflammation is associated with epigenetic change, and it has been suggested that such changes can alter cell phenotypes in ways that contribute to both ongoing inflammation and development of associated pathologies. Here we show that exposure of human gingival fibroblasts to IL-1β increases expression of maintenance methyltransferase DNMT1 but decreases expression of …

The Effects Of A Glp-1 Receptor Agonist On Hba1c And Weight In Comparison To Standard Therapy In A Veteran Population, Isaac Warshawsky

Undergraduate Honors Thesis Collection

Glucagon-Like Peptide 1 Receptor Agonists (GLP-1 RAs) present a novel therapy for use in Type 2 Diabetes Mellitus (T2DM) patients to reduce Hemoglobin A1c (HbA1c) and weight. GLP-1 RAs are costly medications and are not currently on the national formulary for Veterans Affairs medical centers. This study looks to explore the efficacy data in a veteran population.

Evaluation Of Attendance, Patient Satisfaction, Knowledge, And Clinical Outcomes Following Participation In A Group Diabetes Self-Management Education And Support Program In A Primary Care Clinic Setting, Jordan Rauh

Undergraduate Honors Thesis Collection

Introduction: Although group Diabetes Self-Management Education and Support (DSMES) programs have been attempted throughout Community Health Network, no evaluation of the programs had been conducted.

Objectives: The primary objectives of this study were to evaluate attendance, patient satisfaction and change in disease state knowledge following participation in six sessions of a group DSMES program led by a multidisciplinary team. The secondary objective was to evaluate clinical outcomes.

Methods: This retrospective, observational study focused on process improvement evaluating a standardized approach to a group DSMES program beginning in August 2017 within Community Health Network. Eleven courses ran six sessions lasting approximately …

Counseling On Exercise: The Pharmacist's Role, Katelyn Hettinger

Undergraduate Honors Thesis Collection

Only one in three adults in the United States meets the weekly physical activity recommendations, and only 5% of adults are active for at least 30 minutes a day.¹ Exercise is a key component of health and wellness, especially for patients already experiencing comorbid conditions. Exercise has been proven to help alleviate pain in patients experiencing anything from low back pain to fibromyalgia and has been a helpful adjunct in diabetes management.² Pharmacists are in an exciting position to advocate for changes in patients’ lifestyles daily and are in an incredibly accessible position to do so. However, exercise …

The Impact Of Nurse Stress, Job Distress, Job Satisfaction And Mood On Patient Outcomes, Madelynne Wright

Undergraduate Honors Thesis Collection

This study examined the intercorrelations among nursing home healthcare providers’ stress, job distress, job satisfaction, and mood, as well as the relationships between these factors and patient outcomes. Additionally, this study investigated the impact Butler University’s Music First! program had on nurse job perceptions. A total of 63 nurses from 9 long-term care facilities completed questionnaires asking about stress, job distress, and global job satisfaction. Nurses’ depression, anxiety and hostility were also assessed with a 15-item Profile of Mood States questionnaire. Of these 63 nurses, 20 completed the questionnaires at three different time points during the implementation of the Music …