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Full-Text Articles in Pharmacy and Pharmaceutical Sciences
Population Pharmacokinetics And Pharmacodynamics Of Piperacillin In Critically Ill Patients During The Early Phaseof Sepsis, Waroonrat Sukarnjanaset
Population Pharmacokinetics And Pharmacodynamics Of Piperacillin In Critically Ill Patients During The Early Phaseof Sepsis, Waroonrat Sukarnjanaset
Chulalongkorn University Theses and Dissertations (Chula ETD)
Background: Piperacillin/Tazobactam is frequently used for empirical treatment in patients with sepsis. Pathophysiological changes during the early phase of sepsis have significant effects on pharmacokinetic/pharmacodynamic (PK/PD) behaviors. This study aimed to characterize the population PKs of piperacillin and investigate probability of target attainment (PTA) and cumulative fraction of response (CFR) of various dosage regimens in critically ill patients during the early phase of sepsis. Methods: Forty-eight patients treated with piperacillin/tazobactam were recruited. Five blood samples were drawn before and during 0-0.5, 0.5-2, 2-4 and 4-6 or 8 hours after administration. Free piperacillin concentrations were determined using HPLC. Population PKs was …
Preparation And Antimicrobial Activity Of Caprylic Acid Emulsion For Food Coating, Raweewan Bunyovimonnat
Preparation And Antimicrobial Activity Of Caprylic Acid Emulsion For Food Coating, Raweewan Bunyovimonnat
Chulalongkorn University Theses and Dissertations (Chula ETD)
Antimicrobial food coating is the interesting method for food preservation, especially in fruits and vegetables. Previous studies have been reported that the caprylic acid had the antimicrobial activity against a variety of microorganisms. However, little research has been conducted on the use of caprylic acid in the form of food coatings. Therefore, this study aims to determine the optimum conditions for the caprylic acid emulsion preparation and to evaluate the antimicrobial activity of the emulsion, including the effect of food coatings with caprylic acid emulsion on physical properties and microbial growth inhibition. Emulsions containing 4% w/v caprylic acid (oil) and …
Carbohydrate Content Of The Medications For Epileptic Children Treated With Ketogenic Diet At King Chulalongkorn Memorial Hospital, Thanarat Sawangrit
Carbohydrate Content Of The Medications For Epileptic Children Treated With Ketogenic Diet At King Chulalongkorn Memorial Hospital, Thanarat Sawangrit
Chulalongkorn University Theses and Dissertations (Chula ETD)
The purposes of this study were to establish a database of the carbohydrate content of medications and investigate carbohydrate content of medications in epileptic children treated with ketogenic diet (KD) at King Chulalongkorn Memorial Hospital. One hundred sixty-nine KD order forms in 3 events (KD initiation, follow-up visit, and hospital re-admission) for epileptic children whose aged younger than 18 years old during 2009-2017 were selected. Clinical and nutritional data were obtained from medical records and KD order forms from the pediatric nutrition unit. The study showed that oral liquid dosage forms had the highest carbohydrate content in the formulations as …
Physicochemical, Biopharmaceutical And Anti-Hepatocellular Carcinoma Properties Of Curcumin Diethyl Disuccinate, Chawanphat Muangnoi
Physicochemical, Biopharmaceutical And Anti-Hepatocellular Carcinoma Properties Of Curcumin Diethyl Disuccinate, Chawanphat Muangnoi
Chulalongkorn University Theses and Dissertations (Chula ETD)
Curcumin ( Cur) has been reported for an anticancer activity by inducing apoptosis. However, the poor oral bioavailability of Cur due to chemical instability limits itself for further development as a chemotherapeutic agent. Previously, a succinate ester prodrug of Cur named as curcumin diethyl disuccinate (CurDD) has been designed and synthesized to enhance chemical stability of Cur. In this study, physicochemical and biopharmaceutical properties as well as in vitro and in vivo anti-hepatocellular carcinoma activities of CurDD were investigated and compared with those of Cur. CurDD could improve the stability of Cur in buffer pH 7.4 and simulated gastrointestinal fluids. …
Health Policies And Programs Facilitating Access To High-Cost Anticancer Drugs, Chanthawat Patikorn
Health Policies And Programs Facilitating Access To High-Cost Anticancer Drugs, Chanthawat Patikorn
Chulalongkorn University Theses and Dissertations (Chula ETD)
Access to anticancer drugs is limited mainly due to their high cost. To support policymakers in Thailand to develop policies and programs to facilitate better access to high-cost anticancer drugs, global and local evidence are needed. A systematic review of literature was conducted using PubMed, Embase and Web of Science between October 8-11, 2018 to identify peer-reviewed articles published in English from 2000 to 2018. Studies were included if they described health policies or programs facilitating access to high-cost anticancer drugs. Using thematic synthesis, policies and programs were summarized by themes and by income classification of countries. In addition, a …
A Study On The In Vitro Stability And Metabolism Of Curcumin Diethyl Disuccinate In Plasma, Pahweenvaj Ratnatilaka Na Bhuket
A Study On The In Vitro Stability And Metabolism Of Curcumin Diethyl Disuccinate In Plasma, Pahweenvaj Ratnatilaka Na Bhuket
Chulalongkorn University Theses and Dissertations (Chula ETD)
Stability in aqueous media such as buffers and plasma are the one of important properties of drug candidates. Curcumin diethyl disuccinate (CDD), an ester prodrug of curcumin, has been developed to improve the chemical stability of curcumin. In this study, the in vitro stability of CDD in HCl buffer (pH 1.2), acetate buffer (pH 4.5), phosphate buffer (pH 6.8, 7.4 and 8.0) and in vitro metabolism of CDD in rat, dog and human plasma were investigated at 4, 25 and 37 °C. HPLC and nonlinear regression analyses showed that the degradation of CDD in buffers was temperature-dependent and followed pseudo-1st …
Protective Effects Of Eriodictyol On Hydrogen Peroxide-Induced Cell Death In Sh-Sy5y Cells, Saowarose Thongin
Protective Effects Of Eriodictyol On Hydrogen Peroxide-Induced Cell Death In Sh-Sy5y Cells, Saowarose Thongin
Chulalongkorn University Theses and Dissertations (Chula ETD)
Oxidative stress and mitochondrial dysfunction are the major causes of neuronal death in neurodegenerative disorders. Eriodictyol is a flavonoid extracted from the whole plant of Dendrobium ellipsophyllum (thai name: Ueang Thong). The antioxidant and anti-inflammatory effects of eriodictyol have been widely reported. The present study aimed to investigate the protective effect and mechanisms of eriodictyol on hydrogen peroxide (H2O2)-induced cell death in human neuroblastoma SH-SY5Y cells. The protective effect of eriodictyol on cytotoxicity induced by H2O2 was evaluated using resazurin assay. The effects on intracellular reactive oxygen species (ROS) and mitochondria membrane potential were evaluated by 2´-7’ dichlorodihydrofluorescein diacetate (DCFH-DA) …
Effects Of 4,5,4'-Trihydroxy-3,3'-Dimethoxybibenzyl From Dendrobium Ellipsophyllum On Induction Of Apoptosis And Inhibition Of Cancer Stem-Like Cells In Human Lung Cancer H460 Cells, Anirut Hlosrichok
Chulalongkorn University Theses and Dissertations (Chula ETD)
Failure of current chemotherapeutic drugs leads to the recurrence of tumors and increased mortality in lung cancer patients. This study investigated 4,5,4'-trihydroxy-3,3'-dimethoxybibenzyl (TDB), a bibenzyl extracted from Dendrobium ellipsophyllum Tang and Wang as a novel anticancer agent for lung cancer. Selective anticancer activity of TDB against human lung cancer cells was demonstrated with a high half maximal inhibitory concentration (IC50) approximately > 300 µM in dermal papilla cells, while IC50 in human lung cancer H460 cells was approximately 100 ± 5.18 µM. Flow cytometry confirmed that TDB (50 µM) treatment for 24 h, caused significant early and late apoptosis but not …
Effect Of Major Triterpenoids Of Centella Asiatica On Neurite Outgrowth In Neuro-2a Cells, Nonthaneth Nalinratana
Effect Of Major Triterpenoids Of Centella Asiatica On Neurite Outgrowth In Neuro-2a Cells, Nonthaneth Nalinratana
Chulalongkorn University Theses and Dissertations (Chula ETD)
Centella asiatica has been reported to demonstrate neuroprotection and memory improvement in various animal models. Triterpenoid glycosides, madecassoside (MS), asiaticoside (AS), and their aglycones, madecassic acid (MA) and asiatic acid (AA) are considered as major neuroactive constituents in C. asiatica. In this study we aimed to compared MS, AS, MA and AA for their neurite outgrowth activities and mechanisms in Neuro-2a cells. Immunofluorescent staining against ßIII-tubulin showed that MS and AS significantly increased the percentage of neurite-bearing cells and neurite length with higher potency than MA and AA. Our mechanistic studies revealed the differences in signal activation between glycosides and …
Effect Of Ercc1, Xrcc1 And Gstp1 Polymorphisms On Clinical Response And Adverse Events Of Platinum-Based Chemotherapy In Epithelial Ovarian Cancer Patients, Salisa Liblab
Chulalongkorn University Theses and Dissertations (Chula ETD)
Platinum-based chemotherapy is the first line chemotherapy regimen for epithelial ovarian cancer (EOC). Pharmacogenomics is one factor that might affect its efficacy and toxicity. This cohort study was aimed to investigate the association between ERCC1, XRCC1 and GSTP1 polymorphisms on clinical responses and adverse events related to platinum-based chemotherapy in Thai EOC patients. Fifty-two patients with advanced EOC were enrolled in this cohort study. The mean age was 55.7 years old. Genotyping analysis of ERCC1 (C>A, rs3212986), XRCC1 (A>G, rs25487) and GSTP (A>G, rs1695) were performed which allele frequencies were found at 35.6%, 28.9% and 10.6%, respectively. …
Effect Of Bitter Gourd Extracts On The Mutagenicity Of 1-Aminopyrene Reacted With Nitrite Using Ames Test, Korkiat Panpasuk
Effect Of Bitter Gourd Extracts On The Mutagenicity Of 1-Aminopyrene Reacted With Nitrite Using Ames Test, Korkiat Panpasuk
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study was conducted to investigate the mutagenic activity of raw and cooked bitter gourd (Momordica charantia) including raw fruit extract (RW), blanching water (BW), hot water extract from blanched fruit (HB), stir-fried fruit extract (SF), and stir-fried fruit (pre-squeezed with salt) extract (SS). In addition, the effects of extracts in modifying the mutagenic activity of mutagen freshly prepared by the reaction between 1-aminopyrene and sodium nitrite and the effects when adding each extract at the beginning of the same reaction using Ames test on Salmonella typhimurium strains TA98 and TA100 without enzymatic activation were also determined. The data for …
Antioxidant And Antimutagenic Activity Of Extracts From Pericarp And Seed Of Zanthoxylum Limonella Alston, Patamawan Wipoosanapan
Antioxidant And Antimutagenic Activity Of Extracts From Pericarp And Seed Of Zanthoxylum Limonella Alston, Patamawan Wipoosanapan
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study was conducted to investigate the antioxidant and antimutagenic activity of Zanthoxylum limonella seed extract and that of Z. limonella pericarp extract. The total phenolic contents and antioxidant activity assay including DPPH assay and FRAP assay were performed. The results showed that the antioxidant activity of pericarp extract was higher than that of the seed extract. The direct mutagenicity assay was determined, with and without nitrite treatment using the Ames test towards Salmonella typhimurium strains TA98 and TA100. The mutagenic modification assay was performed by measuring the interfering effect of the pericarp extract and the seed extract on the …
Preparation And Antimicrobial Activity Of Caprylic Acid Emulsion For Food Coating, Raweewan Bunyovimonnat
Preparation And Antimicrobial Activity Of Caprylic Acid Emulsion For Food Coating, Raweewan Bunyovimonnat
Chulalongkorn University Theses and Dissertations (Chula ETD)
Antimicrobial food coating is the interesting method for food preservation, especially in fruits and vegetables. Previous studies have been reported that the caprylic acid had the antimicrobial activity against a variety of microorganisms. However, little research has been conducted on the use of caprylic acid in the form of food coatings. Therefore, this study aims to determine the optimum conditions for the caprylic acid emulsion preparation and to evaluate the antimicrobial activity of the emulsion, including the effect of food coatings with caprylic acid emulsion on physical properties and microbial growth inhibition. Emulsions containing 4% w/v caprylic acid (oil) and …
Preparation And Stabilization Of Saccharide Fiber Produced By Centrifugal Spinning, Pattama Donghuntong
Preparation And Stabilization Of Saccharide Fiber Produced By Centrifugal Spinning, Pattama Donghuntong
Chulalongkorn University Theses and Dissertations (Chula ETD)
The objectives of this study were to prepare saccharide fiber via centrifugal spinning and further investigate its stability under stress condition. Different saccharides were chosen and spun via centrifugal force to form thread like matter. The results indicated that sucrose (SC) and trehalose (TH) could be fabricated as fiber whereas other saccharides were not able to form. The formation of fiber mainly involved with glass transition temperature (Tg), molten ability including stability of saccharide after melting. High Tg saccharides were a good candidate on fiber preparation. Homogeneous melting with appreciable stability was another factor affecting on fiber preparation. Decomposing after …
Steady-State Determination Of Continuous Powder Mixing By Near Infrared Spectroscopy As Process Analytical Technology, Katesaraporn Wongves
Steady-State Determination Of Continuous Powder Mixing By Near Infrared Spectroscopy As Process Analytical Technology, Katesaraporn Wongves
Chulalongkorn University Theses and Dissertations (Chula ETD)
Powder mixing is one of an important unit operation in pharmaceutical manufacturing of solid dosage forms. Continuous mixing was developed as an alternative method to reduce disadvantages of traditional batch mixing processes. This process was originally developed using horizontal cylindrical mixer equipped with Process Analytical Technology (PAT) tools for real-time monitoring. In the present study, a vertical cylindrical mixer was developed and evaluated. Near Infrared (NIR) spectra obtained by monitoring the mixing process were pretreated with Multiplicative Scatter Correction (MSC), second derivative treatment and Norris-William smoothing in the 6800 - 8700 cm-1 range before a calibration model using PLS was …
Development Of A Determinant Scale Of PharmacistS Care For Herbal And Dietary Supplement Users, Mohd Shahezwan Abd Wahab
Development Of A Determinant Scale Of PharmacistS Care For Herbal And Dietary Supplement Users, Mohd Shahezwan Abd Wahab
Chulalongkorn University Theses and Dissertations (Chula ETD)
Objective: There is a lack of instrument that is valid and reliable to measure the determinant of pharmacist's care (PCare) for herbal and dietary supplement (HDS) users. The present study aimed to develop scales to measure the determinant of PCare for HDS users and to quantify the practices. The study utilized qualitative and quantitative approaches. Methods: Three scales were developed: Direct TPB scale based on a modified Theory of Planned Behaviour (m-TPB) framework, Indirect TPB scale based on the m-TPB and beliefs elicited from community pharmacists (CPs), and PCare-HDS scale based on findings from the qualitative study. This study involved …
Taxonomy And Secondary Metabolites Of Selected Endophytic Actinomycete Strains, Nattakorn Kuncharoen
Taxonomy And Secondary Metabolites Of Selected Endophytic Actinomycete Strains, Nattakorn Kuncharoen
Chulalongkorn University Theses and Dissertations (Chula ETD)
A total of 50 endophytic actinomycetes isolated from fourteen plant species collected from six provinces of Thailand including Bangkok, Chachoengsao, Chiang Mai, Nakhon Pathom, Prachinburi and Suphan Buri were taxonomically studied relied on their phenotypic and chemotaxonomic characteristics, and the 16S rRNA gene sequence analysis. The isolates were identified as members of the family Streptomycetaceae involving Streptomyces (22 isolates), and the family Micromonosporaceae including Micromonospora (25 isolates), Plantactinospora (1 isolate) and Polymorphospora (1 isolate). Based on phenotypic characteristics, whole genome sequence analysis and DNA-DNA hybridization, 5 novel actinomycete species were proposed consisting of Micromonospora globbae (strain WPS1-2T), Micromonospora azadirachtae (strain …
Development Of In Situ Gel Containing Asiaticoside-Cyclodextrin Complex For Periodontal Tissue Regeneration, Hay Man Saung Hnin Soe
Development Of In Situ Gel Containing Asiaticoside-Cyclodextrin Complex For Periodontal Tissue Regeneration, Hay Man Saung Hnin Soe
Chulalongkorn University Theses and Dissertations (Chula ETD)
Asiaticoside (AS) is active herbal compound isolated from Centella asiatica. It has the potential benefit in promoting type I collagen (COL I) synthesis and osteogenic differentiation in human periodontal ligament cells. However, it has low aqueous solubility which may hamper the bioavailability. Thus, the main objective of this study was to develop the thermoresponsive in situ gels containing AS by using cyclodextrin (CD). To determine the solubility enhancement of AS, phase-solubility profiles were investigated and revealed that CDs enhanced the aqueous solubility of AS. Hydroxypropyl-beta-CD (HPβCD) and sulfobutyl-ether-beta-CD (SBEβCD) were selected due to highly solubilization enhancement of AS. The mucoadhesive …
Chemical Composition And In Vitro Anticancer Activity Of Okra (Albelmoschus Esculentus) Seed Extract To Develop Into Polymeric Micelles, Watcharaphong Chaemsawang
Chemical Composition And In Vitro Anticancer Activity Of Okra (Albelmoschus Esculentus) Seed Extract To Develop Into Polymeric Micelles, Watcharaphong Chaemsawang
Chulalongkorn University Theses and Dissertations (Chula ETD)
The objective of this research was to study the phytochemical composition of the Okra seed extract and its in vitro anticancer activity for future pharmaceutical use. We detected active flavonoids compounds such as isoquercitrin, at an amount of 2.89 ± 1.64 % w/w. Furthermore, antioxidation testing by DPPH assay and ABTS assay, showed values of IC50 = 55.80 ug/ml and 49.04 ug/ml, respectively. The Okra seed extract’s anti-cancer activity was evaluated on cancer cell lines, indicating anticancer properties for certain forms of cancer such as cervical cancer (HeLa), liver cancer (HepG2), and most significantly for breast cancer (MCF-7). The mechanisms …
Topoisomerase I Inhibitory Compounds From The Roots Of Paphiopedilum Callosum, San Yoon Nwe
Topoisomerase I Inhibitory Compounds From The Roots Of Paphiopedilum Callosum, San Yoon Nwe
Chulalongkorn University Theses and Dissertations (Chula ETD)
Paphiopedilum callosum (Rchb.f.) Stein belongs to the Orchidaceae family. The phytochemistry and bioactivity of this plant have not been previously reported. In this study, the chromatographic separation of methanolic extract of P. callosum roots was bioassay-guided by a yeast cell-based assay. One new compound, 3′-hydroxy-2,6,5′-trimethoxystilbene (PC1) and five known compounds including 3′-hydroxy-2,5′-dimethoxystilbene (PC2), 3-methylethergalangin (PC3), galangin (PC4), 2,3′-dihydroxy-5,5′-dimethoxystilbene (PC5) and 2,3′-dihydroxy-5′-methoxystilbene (PC6) were isolated. The chemical structures of these isolated compounds were elucidated and identified on the basis of analysis spectroscopic (ESI-MS, NMR and IR), as well as comparison with those reported in the literature. Their topoisomerase I poison activity …
Neuroprotective Effects Of The Standardized Extract Of Centella Asiatica Eca233 In Rotenone-Induced ParkinsonS Disease Model, Narudol Teerapattarakan
Neuroprotective Effects Of The Standardized Extract Of Centella Asiatica Eca233 In Rotenone-Induced ParkinsonS Disease Model, Narudol Teerapattarakan
Chulalongkorn University Theses and Dissertations (Chula ETD)
The standardized extract of Centella asiatica ECa233 consists of asiaticoside and madecassoside. Previous studies reported various pharmacological effects of, ECa233, especially in the central nervous system disorders. The present study aimed to investigate the neuroprotective effects and protective mechanisms of ECa233 against rotenone-induced neurotoxicity in rats and in neuroblastoma SH-SY5Y cells. In animal study, male Wistar rats were divided into five experimental groups (n=10); control, PD, PD-ECa10, PD-ECa30, and PD-EC100. On days 1-20, control and PD rats received 0.5% carboxymethylcellulose (CMC) (1 mL/kg, p.o.) while ECa233 10, 30 and 100 mg/kg (p.o.) were given to PD-ECa10, PD-ECa30, and PD-EC100, respectively. …
Needs Analysis And Development Of Professional Development Program For Thai Community Pharmacists, Surangkana Puengrung
Needs Analysis And Development Of Professional Development Program For Thai Community Pharmacists, Surangkana Puengrung
Chulalongkorn University Theses and Dissertations (Chula ETD)
Background: Primary care services, as recommended by the National Health Security Office (NHSO), are crucial for Thai community pharmacists. Training needs for the services and a professional development or training program however remained unexplored. Objectives: To analyze community pharmacists' training needs and develop professional development programs to meet their needs. Methods: A mixed-methods study with two phases was conducted during 2018-2019. Phase 1 was involved in the needs assessment using semi-structured interview with 10 key informants who were community pharmacists and stakeholders based on three levels (i.e. tasks, person, and organization) and needs confirmation with a self-administered questionnaire surveyed in …
การศึกษาเภสัชจลนศาสตร์ของสารออกซิเรสเวอราทรอลโดยใช้พิเพอรีนเป็นสารเพิ่มชีวปริมาณออกฤทธิ์ในหนูแรท, ธีรรินทร์ จันทร์แสง
การศึกษาเภสัชจลนศาสตร์ของสารออกซิเรสเวอราทรอลโดยใช้พิเพอรีนเป็นสารเพิ่มชีวปริมาณออกฤทธิ์ในหนูแรท, ธีรรินทร์ จันทร์แสง
Chulalongkorn University Theses and Dissertations (Chula ETD)
สาร Oxyresveratrol เป็นสารสำคัญในแก่นของต้นมะหาด โดยได้มีการศึกษาถึงฤทธิ์ทางเภสัชวิทยาของสาร Oxyresveratrol ในหลายด้าน แต่อย่างไรก็ตามสาร Oxyresveratrol เป็นสารที่มีค่าชีวปริมาณออกฤทธิ์ต่ำจึงเป็นอุปสรรคที่สำคัญในการนำมาพัฒนาเป็นยารับประทาน ดังนั้นจุดประสงค์ในการศึกษานี้ เพื่อศึกษาเภสัชจลนศาสตร์ของสาร Oxyresveratrol ที่เปลี่ยนไปเมื่อให้สาร Piperine เป็นสารเพิ่มชีวปริมาณออกฤทธิ์ในหนูแรท ซึ่งขนาดที่ให้แบ่งเป็นสาร Oxyresveratrol เดี่ยวขนาด 10 mg/kg หรือ สาร Oxyresveratrol ขนาด 10 mg/kg ให้ร่วมกับสาร Piperine 1 mg/kg โดยการบริหารยาทางหลอดเลือดดำ และ สาร Oxyresveratrol เดี่ยวขนาด 100 mg/kg หรือ สาร Oxyresveratrol ขนาด 100 mg/kg ให้ร่วมกับสาร Piperine 10 mg/kg โดยการบริหารยาทางปากจากนั้นเก็บตัวอย่างเลือด, อวัยวะ, ปัสสาวะ และ อุจจาระ ตามเวลาที่กำหนดนำไปวิเคราะห์ด้วยเครื่อง LC-MS/MS เพื่อวิเคราะห์ข้อมูลทางเภสัชจลนศาสตร์ จากผลการศึกษาพบว่าสาร Piperine สามารถเพิ่มค่าชีวปริมาณออกฤทธิ์ของสาร Oxyresveratrol ขึ้นประมาณ 2 เท่า รวมถึงสามารถช่วยเพิ่มค่าความเข้มข้นของสาร Oxyresveratrol สูงสุดในพลาสมาโดยการบริหารทางปากและ เมื่อคำนวณอัตราส่วนปริมาณยาในอวัยวะต่อพลาสมามีค่าประมาณ 10-100 เท่า ซึ่งสอดคล้องกับค่าปริมาณการกระจายยาที่สูง ประมาณ 50 L/kg ในกระบวนการเปลี่ยนแปลงสภาพของสารเมื่อได้รับร่วมกับสาร Piperine มีค่า Conversion ratio ลดลงในทั้งสองรูปแบบการบริหาร โดยสรุปสาร Piperine สามารถปรับปรุงค่าทางเภสัชจลนศาสตร์ของสาร Oxyresveratrol ได้ทั้งในทั้งสองรูปแบบการบริหารยา การศึกษาทางเภสัชจลนศาสตร์นี้จึงสามารถนำมาเป็นข้อมูลในการพัฒนาสาร Oxyresveratrol เพื่อใช้ประโยชน์ต่อไปได้
ฤทธิ์ต้านมะเร็งของเอทท็อกซี่แมนโซโนนจีต่อเซลล์มะเร็งเต้านม, พิริยา ชนสุต
ฤทธิ์ต้านมะเร็งของเอทท็อกซี่แมนโซโนนจีต่อเซลล์มะเร็งเต้านม, พิริยา ชนสุต
Chulalongkorn University Theses and Dissertations (Chula ETD)
ผู้ป่วยมะเร็งเต้านมที่มีการแสดงออกของตัวรับเอสโตรเจนในระยะลุกลามร้อยละ 40 สามารถกลับเป็นมะเร็งซ้ำเนื่องจากมีการดื้อยา tamoxifen แพทย์จึงเปลี่ยนไปใช้ยาต้านฮอร์โมนกลุ่มอื่น แต่ยังเกิดการดื้อยาและมีผลข้างเคียงที่รุนแรง ดังนั้นการนำสารจากธรรมชาติมาพัฒนาเป็นยาที่มีฤทธิ์ยับยั้งการเจริญของเซลล์มะเร็งจึงเป็นแนวทางที่อาจลดปัญหาดื้อยาได้ สารสกัดจากต้นจันทน์หอม (Mansonia Gagei Drumm) ยับยั้งการเจริญของเซลล์มะเร็งได้หลายชนิด โดย mansonone G (MG) สารสำคัญที่แยกจากสารสกัดสามารถแย่งจับกับตัวรับเอสโตรเจนได้ ในการศึกษานี้จึงทำการทดสอบฤทธิ์อนุพันธ์ MG คือ Ethoxy mansonone G (EMG) สารกึ่งสังเคราะห์ในการยับยั้งการเจริญ การลุกลามและการยับยั้งการเจริญแบบไร้การยึดเกาะ พบว่าเมื่อเพิ่มความเข้มข้นและระยะเวลาในการทดสอบ EMG สามารถยับยั้งการเจริญในเซลล์มะเร็งเต้านมชนิดที่ไม่ดื้อยา (MCF-7) และเซลล์มะเร็งเต้านมที่ดื้อต่อยาต้านฮอร์โมน (MCF-7/LCC2, MCF-7/LCC9) อย่างมีนัยสำคัญทางสถิติและมีความเป็นพิษน้อยกว่ายาเคมีบำบัดในเซลล์ไฟโปรบลาสต์ และพบว่ากลไกการออกฤทธิ์ของ EMG ในการยับยั้งการเจริญของเซลล์ MCF-7 ส่วนหนึ่งผ่านการลดการแสดงออกของยีนที่เป็นเป้าหมายของตัวรับเอสโตรเจนและสาร EMG ยังสามารถลดการแสดงออกของยีนที่เกี่ยวกับการดื้อต่อยา tamoxifen ใน MCF-7/LCC2 และ MCF-7/LCC9 นอกจากนี้ EMG ยังยับยั้งการลุกลามของ MCF-7/LCC2 และ MCF-7/LCC9 โดยลดการแสดงออกของ MMP-9 และ uPA ทั้งยังสามารถลดการเจริญแบบไร้การยึดเกาะผ่านการลดจำนวน ขนาดของ colony ใน MCF-7/LCC2 และ MCF-7/LCC9 สรุปว่าสาร EMG มีคุณสมบัติในการต้านมะเร็งเต้านมชนิดไม่ดื้อยาและชนิดที่ดื้อต่อยาต้านฮอร์โมน ต้านการลุกลาม รวมไปถึงลดการเจริญแบบไร้การยึดเกาะของ MCF-7/LCC2 และ MCF-7/LCC9 ซึ่งอาจนำไปพัฒนาเป็นยาชนิดใหม่ในการรักษาผู้ป่วยมะเร็งเต้านมที่ดื้อต่อยาต้านฮอร์โมน
เภสัชจลนศาสตร์ของตำรับยาทาผิวหนัง Finasteride ในหนูแรท, สุธีรา ภูมิภักดี
เภสัชจลนศาสตร์ของตำรับยาทาผิวหนัง Finasteride ในหนูแรท, สุธีรา ภูมิภักดี
Chulalongkorn University Theses and Dissertations (Chula ETD)
ยาฟิแนสเทอไรด์ (finasteride) เป็นยาในกลุ่ม 5-alpha reductase inhibitor ใช้รักษาโรคผมร่วงทางพันธุกรรมในเพศชาย โดยยาฟิแนสเทอไรด์อาจทำให้เกิดผลข้างเคียงหลายประการได้แก่ การเสื่อมสมรรถภาพทางเพศ ปริมาณน้ำอสุจิลดลง ดังนั้นควรมีการถูกใช้ในรูปแบบยาทาหนังศีรษะเพื่อลดผลข้างเคียงดังกล่าว โดยการศึกษานี้มีวัตถุประสงค์เพื่อศึกษาระดับยาฟิแนสเทอไรด์ในเลือดและในชั้นผิวหนัง หลังการให้ยาความแรง 0.5% ด้วยการทาผิวหนังเพียงครั้งเดียว (single dose) และแบบทาผิวหนังหลายครั้ง (repeated dose) บนพื้นที่ 4 ตารางเซนติเมตรของหนูแรทเพศผู้สายพันธุ์ Sprage-Dawley โดยมีการเก็บพลาสมาและผิวหนังภายใน 24 ชั่วโมง หลังการทายาครั้งสุดท้ายและสกัดยาออกมาเพื่อตรวจวัดระดับยาฟิแนสเทอไรด์ด้วยเครื่อง LC-MS/MS ผลการศึกษาแสดงให้เห็นว่า ระดับยาฟิแนสเทอไรด์ในพลาสมาหลังการให้ยาด้วยการทาผิวหนังเพียงครั้งเดียวมีแนวโน้มการดูดซึมเข้ากระแสเลือดเพิ่มขึ้นตามปริมาตรยาที่ทา โดยเริ่มตรวจพบระดับยาที่เวลา 4 ชั่วโมง หลังทายาในปริมาตร 300 ไมโครลิตร เพียงครั้งเดียว แต่ไม่พบระดับยาในพลาสมาของกลุ่มที่ได้รับยาปริมาตร 75 หรือ 150 ไมโครลิตร นอกจากนี้การทายาซ้ำวันละ 1 ครั้งและทายาซ้ำวันละ 2 ครั้ง ติดต่อกัน 7 วัน ไม่มีนัยสำคัญต่อระดับยาในพลาสมา ซึ่งเห็นได้ว่ายาฟิแนสเทอไรด์ถูกดูดซึมเข้ากระแสเลือดได้น้อยหลังการให้ยาด้วยการทาผิวหนัง สำหรับอัตราส่วนระหว่างระดับยาในผิวหนังและระดับยาในพลาสมาที่เวลา 24 ชั่วโมง หลังได้รับยาทางปากเพียงครั้งเดียวมีค่าประมาณ 2.6 เท่า โดยพบอัตราส่วนระหว่างระดับยาในผิวหนังบริเวณที่ไม่สัมผัสยา (control sites) และระดับยาในพลาสมาเกินกว่า 2.6 เท่า ในหนูแรทกลุ่มที่ได้รับยาทางผิวหนังทุกกลุ่ม ทั้งนี้ค่าที่ตรวจพบระดับยาในพลาสมามีความแปรปรวนสูงมาก อย่างไรก็ตามผลการศึกษานี้แสดงให้เห็นว่า ยาฟิแนสเทอไรด์สามารถสะสมอยู่ในผิวหนังชั้นหนังแท้ (dermis) และถูกดูดซึมเข้าสู่กระแสเลือดได้น้อยหลังให้ยาด้วยการทาผิวหนัง ทั้งนี้จำนวนครั้งการทาทั้งการทาซ้ำวันละ 1 ครั้งและการทาซ้ำวันละ 2 ครั้งอาจไม่มีผลอย่างมีนัยสำคัญต่อการดูดซึมยาเข้าสู่กระแสเลือด
Preparation And Antimicrobial Activity Of Caprylic Acid Emulsion For Food Coating, Raweewan Bunyovimonnat
Preparation And Antimicrobial Activity Of Caprylic Acid Emulsion For Food Coating, Raweewan Bunyovimonnat
Chulalongkorn University Theses and Dissertations (Chula ETD)
Antimicrobial food coating is the interesting method for food preservation, especially in fruits and vegetables. Previous studies have been reported that the caprylic acid had the antimicrobial activity against a variety of microorganisms. However, little research has been conducted on the use of caprylic acid in the form of food coatings. Therefore, this study aims to determine the optimum conditions for the caprylic acid emulsion preparation and to evaluate the antimicrobial activity of the emulsion, including the effect of food coatings with caprylic acid emulsion on physical properties and microbial growth inhibition. Emulsions containing 4% w/v caprylic acid (oil) and …
Taxonomy And Secondary Metabolites Of Selected Endophytic Actinomycete Strains, Nattakorn Kuncharoen
Taxonomy And Secondary Metabolites Of Selected Endophytic Actinomycete Strains, Nattakorn Kuncharoen
Chulalongkorn University Theses and Dissertations (Chula ETD)
A total of 50 endophytic actinomycetes isolated from fourteen plant species collected from six provinces of Thailand including Bangkok, Chachoengsao, Chiang Mai, Nakhon Pathom, Prachinburi and Suphan Buri were taxonomically studied relied on their phenotypic and chemotaxonomic characteristics, and the 16S rRNA gene sequence analysis. The isolates were identified as members of the family Streptomycetaceae involving Streptomyces (22 isolates), and the family Micromonosporaceae including Micromonospora (25 isolates), Plantactinospora (1 isolate) and Polymorphospora (1 isolate). Based on phenotypic characteristics, whole genome sequence analysis and DNA-DNA hybridization, 5 novel actinomycete species were proposed consisting of Micromonospora globbae (strain WPS1-2T), Micromonospora azadirachtae (strain …
Taxonomy And Secondary Metabolites Of Selected Endophytic Actinomycete Strains, Nattakorn Kuncharoen
Taxonomy And Secondary Metabolites Of Selected Endophytic Actinomycete Strains, Nattakorn Kuncharoen
Chulalongkorn University Theses and Dissertations (Chula ETD)
A total of 50 endophytic actinomycetes isolated from fourteen plant species collected from six provinces of Thailand including Bangkok, Chachoengsao, Chiang Mai, Nakhon Pathom, Prachinburi and Suphan Buri were taxonomically studied relied on their phenotypic and chemotaxonomic characteristics, and the 16S rRNA gene sequence analysis. The isolates were identified as members of the family Streptomycetaceae involving Streptomyces (22 isolates), and the family Micromonosporaceae including Micromonospora (25 isolates), Plantactinospora (1 isolate) and Polymorphospora (1 isolate). Based on phenotypic characteristics, whole genome sequence analysis and DNA-DNA hybridization, 5 novel actinomycete species were proposed consisting of Micromonospora globbae (strain WPS1-2T), Micromonospora azadirachtae (strain …
Anticancer Effects Of Mansonone G Derivative On Human Colorectal Cancer Cells, Savinee Chanvijit
Anticancer Effects Of Mansonone G Derivative On Human Colorectal Cancer Cells, Savinee Chanvijit
Chulalongkorn University Theses and Dissertations (Chula ETD)
Mansonone G (MG), 1,2-naphthoquinone, isolated from the heartwood of Mansonia gagei Drumm (Chan-Cha-Mod), exhibited several pharmacological effects including anti-bacterial, anti-estrogenic and anti-adipogenic effects. However, anticancer activity of MG and its derivatives on colorectal cancer (CRC) has never been investigated. Therefore, the objective of this study was to investigate the cytotoxic effect of MG and its derivatives and to determine the mechanism(s) underlying cytotoxicity of the most potent MG derivative on two CRC cell lines, HCT-116 cells carrying p53 wild-type and HT-29 cells carrying p53 mutant. In the present study, MG and its derivatives could inhibit viability of HCT-116 and HT-29 …
Pharmacokinetic Study Of Cucurbitacin B From Trichosanthes Cucumerina L. In Rats, Natthaphon Hunsakunachai
Pharmacokinetic Study Of Cucurbitacin B From Trichosanthes Cucumerina L. In Rats, Natthaphon Hunsakunachai
Chulalongkorn University Theses and Dissertations (Chula ETD)
Cucurbitacin B is a triterpenoid, majorly found in Cucurbitaceae family, particularly in the Trichosanthes cucumerina L. fruits. The pharmacological properties of cucurbitacin B have been studied for decades, particularly an anti-tumor and anti-inflammatory activity. However, the pharmacokinetic profile of this compound is still limited and the investigation is needed for further pharmaceutical product development. This study aimed to investigate the pharmacokinetic profile of cucurbitacin B after administering the compound at different doses and routes to the rats. Male Wistar rats, weighed approximately 400-500 g, were treated with cucurbitacin B extracted from Trichosanthes cucumerina L. The cucurbitacin B was administered at …