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Full-Text Articles in Pharmacy and Pharmaceutical Sciences
Effects Of Lipids And Surfactants On The Characteristics Of Asiatic Acid-Loaded Solid Lipid Microparticles, Jatuporn Witarat
Effects Of Lipids And Surfactants On The Characteristics Of Asiatic Acid-Loaded Solid Lipid Microparticles, Jatuporn Witarat
Chulalongkorn University Theses and Dissertations (Chula ETD)
The objective of this study was to evaluate the effects of solid lipids: beeswax and cetyl alcohol, and surfactants: Tween 80®, soybean lecithin and poloxamer 188 on the characteristics of solid lipid microparticles containing asiatic acid (AASLM). The AASLM were prepared from 10% or 15% of beeswax or cetyl alcohol together with 3% of surfactant F1-F12) by melt dispersion cum freeze-drying technique to obtain a solid dosage form. The physicochemical characteristics of the AASLM powders, including appearance, surface morphology, particle size, %labeled amount of asiatic acid (AA) and %entrapment efficiency (EE) were evaluated. The results revealed that AASLM prepared from …
Effect Of Vernonia Cinerea Lozenges As A Smoking Cessation Aid In Patients With Non-Communicable Diseases, Krittin Bunditanukul
Effect Of Vernonia Cinerea Lozenges As A Smoking Cessation Aid In Patients With Non-Communicable Diseases, Krittin Bunditanukul
Chulalongkorn University Theses and Dissertations (Chula ETD)
Background: This study aims to investigate the effect of Vernonia Cinerea (VC) lozenges on smoking cessation when compared with a placebo. Methods: This study is designed as a randomized, paralleled, double-blinded, controlled trial. Ninety-three patients who are the age 18 years or over at five hospitals and two community pharmacies were randomized to receive VC lozenges (n=45) or placebo (n=48) for 12 weeks. The primary outcome in this study is the continuous abstinence rate (CARs) at 4 and 12 weeks after the quit date. Results: There was no statistically significant difference in CARs rates at week 4 in the study …
Stimulation Of Immune Response In Mice Using The Plant-Produced Sars-Cov-2 S1 Subunit Protein Linked To The Fc Region, Chalisa Panapitakkul
Stimulation Of Immune Response In Mice Using The Plant-Produced Sars-Cov-2 S1 Subunit Protein Linked To The Fc Region, Chalisa Panapitakkul
Chulalongkorn University Theses and Dissertations (Chula ETD)
Coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 made a devastating impact worldwide, and virus management is challenging owing to a lack of specific therapies or affordable vaccines, especially in low-and middle-income countries. Hence a low-cost and efficient vaccine is necessary. In the present study, the immunogenicity of the plant-produced S1 subunit protein of SARS-CoV-2 was investigated in order to utilize it as a subunit vaccine. The SARS-CoV-2 S1 subunit protein was fused with the Fc region of human immunoglobulin (IgG1) and expressed in tobacco plants in species of Nicotiana benthamiana plants by agroinfiltration. The …
Compounds With Anti-Neuroinflammatory Activity From Aerides Falcata, Bachtiar Rivai
Compounds With Anti-Neuroinflammatory Activity From Aerides Falcata, Bachtiar Rivai
Chulalongkorn University Theses and Dissertations (Chula ETD)
In this study, a plant from the Orchidaceae family, Aerides falcata, was investigated for its chemical constituents and anti-neuroinflammatory activity. A total of ten compounds were isolated and characterized. The isolated compounds included a new compound which was named aerifalcatin and nine known compounds: n-eicosyl-trans-ferulate, denthyrsinin, 2,4-dimethoxy-3,7-dihydroxyphenanthrene, 2,7-dihydroxy-3,4,6-trimethoxyphenanthrene, 3,7-dihydroxy-2,4,6-trimethoxyphenanthrene, agrostonin, syringaresinol, trans-n-feruloyltyramine, and trans-n-coumaroyltyramine. All the isolated compounds were evaluated for their anti-neuroinflammatory activity, except for trans-n-coumaroyltyramine, which was excluded due to its insufficient amount. In vitro testing was conducted on LPS-induced BV2 microglia cells to evaluate their potential anti-neuroinflammatory activity using NO inhibition model. Minocycline, a neuroinflammatory modulator, was …
Anti-Adipogenic Activity Of 4,5,4'-Trihydroxy-3,3'-Dimethoxybibenzyl From Dendrobium Ellipsophyllum In 3t3-L1 Preadipocytes, Hnin Ei Ei Khine
Anti-Adipogenic Activity Of 4,5,4'-Trihydroxy-3,3'-Dimethoxybibenzyl From Dendrobium Ellipsophyllum In 3t3-L1 Preadipocytes, Hnin Ei Ei Khine
Chulalongkorn University Theses and Dissertations (Chula ETD)
Obesity and its global prevalence have become a threat to human health due to not only the abnormal lipid accumulation in the body as a result of aberrant expression levels in adipocyte differentiation and lipogenesis but also the underdeveloped pharmacotherapy. Recently, the bioactive compounds from the natural products have gained a lot of attention due to undertaking prophylactic and therapeutic effects on obesity. Here, we probed how 4,5,4′-trihydroxy-3,3′-dimethoxybibenzyl (TDB) extracted from an Thai orchid (Dendrobium ellipsophyllum) could exert its roles on the differentiation and function of murine (3T3-L1) and human (PCS-210-010) preadipocytes and offer some implications to modulate obesity. Cytotoxic …
Free Radical Scavengers From Dendrobium Virgineum, Pongsawat Panuthai
Free Radical Scavengers From Dendrobium Virgineum, Pongsawat Panuthai
Chulalongkorn University Theses and Dissertations (Chula ETD)
Free radicals are unstable, highly reactive, molecules which can cause several degenerative diseases such as mutagenesis and carcinogenesis. This research aims to study the chemical constitutents of Dendrobium virgineum and their antioxidant activity in 3 different test models including DPPH radical scavenging assay, superoxide radical scavenging activity assay and oxygen radical absorbance capacity assay. Nine compounds were isolated and determined to be two new compounds named 2,8-dimethoxy-9,10-dihydro-4,5-phenanthrenediol and 2,5-dimethoxy-7-hydroxy-9,10-dihydro-1,4-phenanthrenequinone, together with seven known compounds, which are 2-methoxy-9,10-dihydro-4,5-phenanthrenediol, gigantol, 5-methoxy-7-hydroxy-9,10-dihydro-1,4-phenanthrenequinone, p-coumaric acid, tristin, 2,5,7-trihydroxy-4-methoxy-9,10-dihydrophenanthrene and 9,10-dihydro-2,4,7-phenanthrenetriol. The result obtained from the three testing models revealed that 2,5,7-trihydroxy-4-methoxy-9,10-dihydrophenanthrene showed strongest antioxidant activity. …
Production Of Pd-L1 Immune Checkpoint Inhibitor In Nicotiana Benthamiana, Thareeya Phetphoung
Production Of Pd-L1 Immune Checkpoint Inhibitor In Nicotiana Benthamiana, Thareeya Phetphoung
Chulalongkorn University Theses and Dissertations (Chula ETD)
Immune checkpoint antibodies disrupt the binding of receptor-ligand pairs which regulate immune response in cancer treatment. PD-1/PD-L1 pathway has become one target of the cancer immunotherapy approaches. Atezolizumab, the first FDA-approved antibody to PD-L1 in the treatment of metastatic urothelial, non-small cell lung, small cell lung, and hepatocellular cancers, is expressed in Chinese Hamster Ovary (CHO) cell lines with several limitations, i.e., high costs of production, limited-capacity yields, and pathogen risks. To overcome these drawbacks, the transient expression in Nicotiana benthamiana leaves, which provide expandable scalability and inexpensive costs, was investigated by co-infiltration of Agrobacterium tumefaciens GV3101 cultures harboring Atezolizumab …
Titania Nanosheets Induce Anticancer Activityin Human Non-Small Cell Lung Cancer Cells, Rapeepun Soonnarong
Titania Nanosheets Induce Anticancer Activityin Human Non-Small Cell Lung Cancer Cells, Rapeepun Soonnarong
Chulalongkorn University Theses and Dissertations (Chula ETD)
Metal nanomaterials can enhance the efficacy of current cancer therapies. Here, we show that Ti0.8O2 nanosheets cause cytotoxicity in several lung cancer cells but not in normal cells. The nanosheet-treated cells showed certain apoptosis characteristics. Protein analysis further indicated the activation of the p53-dependent death mechanism. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) analyses revealed the cellular uptake of the nanosheets and the induction of cell morphological change. Ti0.8O2 nanosheets induce apoptosis through a molecular mechanism involving peroxynitrite (ONOO−) generation. As peroxynitrite is known to be a potent inducer of S-nitrosylation, we further found that the nanosheets mediated …
Neuroprotective Effect Of Intranasal Delivery Of Asiatic Acid Against Amyloid Beta1-42-Induced Alzheimer's Disease In Mice, Ridho Islamie
Neuroprotective Effect Of Intranasal Delivery Of Asiatic Acid Against Amyloid Beta1-42-Induced Alzheimer's Disease In Mice, Ridho Islamie
Chulalongkorn University Theses and Dissertations (Chula ETD)
Asiatic acid (AA) is an active natural compound that can be derived from Centella asiatica extract. Several pharmacological studies were reported that AA has potential as neuroprotector in the central nervous system disorders. AA penetration in the brain was limited due to its low bioavailability. Nasal delivery system of solid lipid nanoparticles (SLNs) loaded-AA was developed to increase AA penetration in the brain. The current study aimed to investigate neuroprotective effect and protective mechanisms of AA in SLNs by nasal-to-brain delivery technique against Aβ1–42 induced memory impairment by in vivo study. Male ICR mice were divided into six groups (n=11-12/group); …
Photochemical Reaction Of Renieramycins And Evaluation Of Cytotoxicity Against Lung Cancer Cells, Suwimon Sinsook
Photochemical Reaction Of Renieramycins And Evaluation Of Cytotoxicity Against Lung Cancer Cells, Suwimon Sinsook
Chulalongkorn University Theses and Dissertations (Chula ETD)
Lung cancer causes more deaths than any other type of cancers, with the majority of patients diagnosed with non-small cell lung cancer (NSCLC). Lung cancer is a difficult disease to treat because of the drug resistant problem. Therefore, there is an immediate need for effective, safe, and easily accessible anti-lung cancer drug. Ecteinascidin 743 (Trabectedin or Yondelis®), a marine-derived tetrahydroisoquinoline alkaloid, is an approved drug for treating human lung cancer. This research was studied on the semi-syntheses of new renieramycins derivatives with similar structures to above drug for discovery and development of potent NSCLC cytotoxic agents. Renieramycins was extracted from …
A Cost-Utility Analysis Comparing Transcatheter And Surgical Pulmonary Valve Replacement Among Indonesia Patients With A History Of Newborn Right Ventricular Outflow Tract Correction, Andi Nurul Annisa
Chulalongkorn University Theses and Dissertations (Chula ETD)
Transcatheter pulmonary valve replacement (TPVR) was launched since 2000. It indicated for cardiac patients who have the right ventricular outflow tract obstruction (RVOT) and a prior surgery history. TPVR have a shorter hospital stay, fewer complications, and a less-invasive method. However, TPVR is more expensive than surgical pulmonary valve replacement (SPVR). This raises access concerns for low income countries. There are no cost-effectiveness studies in Indonesia, and limited efficiency evidence due to rare disorder. It is a challenge in building the appropriate access strategies. Therefore, this study aims to investigate the cost-utility, resulted in total costs, quality-adjusted life-years (QALYs), life-years …
Financial Feasibility Study Of Incremental Modified Drugs Development By Domestic Pharmaceutical Industry, Manthana Laichapis
Financial Feasibility Study Of Incremental Modified Drugs Development By Domestic Pharmaceutical Industry, Manthana Laichapis
Chulalongkorn University Theses and Dissertations (Chula ETD)
The Thai pharmaceutical industry aims to achieve self-reliance in vaccines, drugs, and biologics as per the National Strategic Master Plan. Challenges include low research capacity and higher drug imports compared to domestic production. To promote self-reliance, more research on incrementally modified drugs (IMDs) is essential. This study analyzes the financial feasibility of developing IMD dosage forms for policy and investment decisions. A mixed-method approach, including type selection, investment models, cost structures, and financial analysis, was employed. Results favor sustained release, oro-dispersible tablet, and nasal spray formulations. IMD research and development took 7 to 13 years due to formulation complexity and …
A Situation Analysis Of The Outpatient Dispensing Service Patterns Among Hospitals In Thailand, Trisak Chetsurakarn
A Situation Analysis Of The Outpatient Dispensing Service Patterns Among Hospitals In Thailand, Trisak Chetsurakarn
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study aims to examine outpatient dispensing patterns in Thai hospitals, focusing on the current situation. The research explores the structures, processes, outputs, and environmental factors of alternative outpatient dispensing services. Surveys were conducted through phone interviews and in-depth interviews with pharmacy department heads using structured interviews with accurate content. Key findings from 197 participating hospitals revealed that 97.35% of interviewed hospitals offered at least one alternative outpatient dispensing service. The most popular method was home delivery (88.6%), followed by sub-district health promoting hospital medication pick-up service (45.1%), hospital refill service (44.7%), community pharmacy medication pick-up service (26.6%), and drive-thru …
Economic Burden Of Snakebite And Cost-Effectiveness Of Improving Access To Snake Antivenom In Asean Countries, Chanthawat Patikorn
Economic Burden Of Snakebite And Cost-Effectiveness Of Improving Access To Snake Antivenom In Asean Countries, Chanthawat Patikorn
Chulalongkorn University Theses and Dissertations (Chula ETD)
The Association of Southeast Asian Nations (ASEAN) countries are among the tropical regions with disproportionately high incidence of snakebite. Understanding the snakebite and antivenom market situation and burden of snakebite is crucial for developing evidence-based strategies to pursue the goal set by the World Health Organization (WHO) to halve morbidity and mortality of snakebite by 2030. Firstly, we systematically review 23 cost of illness studies and 3 economic evaluations. Economic burdens of snakebite were underestimated and not extensively studied. Majority of studies only provided direct costs of snakebite patients presented to the hospitals. Thus, hospital data should be used to …
Anti-Inflammatory Compounds In Human Peripheral Blood Mononuclear Cells From Dendrobium Species, Virunh Kongkatitham
Anti-Inflammatory Compounds In Human Peripheral Blood Mononuclear Cells From Dendrobium Species, Virunh Kongkatitham
Chulalongkorn University Theses and Dissertations (Chula ETD)
In these studies, four new compounds including three phenanthrene derivatives (dendrocrumenols A, B and D) and one fluorenone (dendrocrumenol C) and four known compounds including gigantol, 3,7-dihydroxy-2,4,8-trimethoxyphenanthrene, densiflorol B and cypripedin were isolated from Dendrobium crumenatum Sw. Each structure of isolated compounds from D. crumenatum was elucidated by spectroscopic data. These isolated compounds were then evaluated for anti-inflammatory effects in human peripheral blood mononuclear cells (PBMCs). Among the tested compounds, dendrocrumenols B and D showed the most promising anti-inflammatory effects through inhibition of tumor necrosis factor tumor (TNF) and interleukin-2 (IL-2) in monocytes and T cells. The deep immune profiling …
Chemical Constituents And Nitric Oxide Inhibitory Activity Of Capparis Micracantha And Maerua Siamensis, Sasiwimon Nukulkit
Chemical Constituents And Nitric Oxide Inhibitory Activity Of Capparis Micracantha And Maerua Siamensis, Sasiwimon Nukulkit
Chulalongkorn University Theses and Dissertations (Chula ETD)
The chemical investigation of two plants in Capparaceae family, which are Capparis micracantha and Maerua siamensis, leads to the isolation of 13 compounds. The structures of the isolates were elucidated using spectroscopy techniques and comparison of the previous reports. Of five known compounds from the stems of C. micracantha were an indole alkaloid (methyl 6-methoxy-3-indolecarbonate), a benzoic acid derivative (vanillic acid), a lignan [(−)-syringaresinol], and two stilbene dimers [(+)-ampelopsin A and (−)-pauciflorol E]. In addition, eight new indole alkaloids named (+)-maeruanitrile A, maeruanitrile B, maeroximes A-C and maeruabisindoles A-C were isolated from the roots of M. siamensis,. All isolates were …
Development Of Surrogate Sars-Cov-2 Virus Neutralization Test Method Using Plant-Produced Recombinant Proteins, Perawat Jirarojwattana
Development Of Surrogate Sars-Cov-2 Virus Neutralization Test Method Using Plant-Produced Recombinant Proteins, Perawat Jirarojwattana
Chulalongkorn University Theses and Dissertations (Chula ETD)
Detecting immunity against SARS-CoV-2 is vital for evaluating vaccine response and natural infection, but a conventional virus neutralization test (cVNT) requires BSL3 and live viruses, and a pseudo-virus neutralization test (pVNT) needs specialized equipment and trained professionals in BSL2. The surrogate virus neutralization test (sVNT) was developed to overcome these limitations. This study explored the use of angiotensin converting enzyme (ACE2) produced from Nicotiana benthamiana for the development of an affordable neutralizing antibodies detection assay. The results showed that the plant-produced ACE2-His can bind to the receptor binding domain (RBD) of the SARS-CoV-2, and was used to develop sVNT with …
การพัฒนาโปรแกรมการตรวจติดตามสภาวะแวดล้อมในห้องสะอาดสำหรับยาฉีดด้วยแนวทางการประเมินความเสี่ยง, กิ่งกาญจน์ ศิริมัย
การพัฒนาโปรแกรมการตรวจติดตามสภาวะแวดล้อมในห้องสะอาดสำหรับยาฉีดด้วยแนวทางการประเมินความเสี่ยง, กิ่งกาญจน์ ศิริมัย
Chulalongkorn University Theses and Dissertations (Chula ETD)
การตรวจติดตามสภาวะแวดล้อมมีบทบาทสำคัญในการสร้างความมั่นใจต่อระดับความสะอาดของสภาพแวดล้อมในระหว่างกระบวนการผลิต โดยเฉพาะอย่างยิ่งในการผลิตผลิตภัณฑ์ประเภทยาฉีด โดยควรต้องกำหนดให้มีโปรแกรมการตรวจติดตามสภาวะแวดล้อมอย่างเป็นประจำ เพื่อลดความเสี่ยงของการปนเปื้อนจากเชื้อจุลินทรีย์และอนุภาคสิ่งแปลกปลอมอื่นๆ ในการศึกษาวิจัยนี้มีวัตถุประสงค์เพื่อพัฒนาโปรแกรมการตรวจติดตามสภาวะแวดล้อมตามแนวทางการประเมินความเสี่ยงในห้องสะอาดสำหรับยาฉีดของแผนกบรรจุผลิตภัณฑ์ ศูนย์ผลิตผลิตภัณฑ์จากพลาสมา สภากาชาดไทย จำนวน 13 ห้อง แนวทางการจัดการความเสี่ยงคุณภาพอ้างอิงตาม International Council for Harmonization Q9 โดยใช้เครื่องมือ Risk ranking and filtering ในการประเมินและจัดระดับความเสี่ยงเพื่อกำหนดความถี่และตำแหน่งในการตรวจติดตามสภาวะแวดล้อม สำหรับขั้นตอนการระบุความเสี่ยง มีการประเมินความเสี่ยงเพื่อค้นหาปัจจัยที่อาจทำให้เกิดการปนเปื้อนจากเชื้อจุลินทรีย์ด้วย Fishbone diagram ในการวิเคราะห์ความเสี่ยง ได้ทำการเลือกปัจจัยที่จะส่งกระทบผลต่อคุณภาพและความปลอดภัยของผลิตภัณฑ์ ในแง่ของความรุนแรงและโอกาสที่จะเกิดการปนเปื้อน ปัจจัยที่นำมาพิจารณา ได้แก่ กิจกรรมการผลิต, ระยะเวลาที่มีการปฏิบัติงาน, ขั้นตอนกระบวนการผลิต, ความยากง่ายต่อการทำความสะอาดอุปกรณ์, ความถี่ในการทำความสะอาดและกำจัดเชื้อโรค, ข้อมูลประวัติแนวโน้มของการตรวจติดตามสภาวะแวดล้อม, จำนวนบุคลากรที่ปฏิบัติงาน และการมีท่อเดรนหรือท่อน้ำในพื้นที่ ทั้งนี้เกณฑ์และคะแนนของแต่ละปัจจัยจะถูกกำหนดและประเมินโดยคณะทำงานประเมินความเสี่ยง เพื่อคำนวณค่าความเสี่ยงและจัดระดับได้เป็น 5 ระดับ แนวทางการประเมินความเสี่ยงนำไปสู่โปรแกรมการตรวจติดตามสภาวะแวดล้อมใหม่ ซึ่งมีความถี่ในการตรวจติดตามสภาวะแวดล้อมของแต่ละพื้นที่ได้เป็นสัปดาห์ละครั้ง จำนวน 5 ห้อง สองสัปดาห์ครั้ง จำนวน 1 ห้อง และสามเดือนครั้ง จำนวน 7 ห้อง สำหรับตำแหน่งและวิธีการทดสอบที่ประเมินได้ คือ Particle count 26 ตำแหน่ง, Volumetric air sampling 26 ตำแหน่ง, Settle plate 29 ตำแหน่ง และ Contact plate 33 ตำแหน่ง ในขั้นตอนการทบทวนความเสี่ยงพบว่าหลังจากเปลี่ยนโปรแกรมการตรวจติดตามสภาวะแวดล้อมใหม่ ผลการตรวจยังคงผ่านตามเกณฑ์ที่กำหนด และเมื่อพิจารณาจากการคำนวณ %Contamination recovery rate (CRR) พบว่ายังคงผ่านตามเกณฑ์ที่กำหนดเช่นเดียวกัน ดังนั้นจึงไม่ได้มีผลกระทบต่อคุณภาพของผลิตภัณฑ์หรือระดับการปนเปื้อนในห้องสะอาด โดยรวมแล้วความสำเร็จของโปรแกรมการตรวจติดตามสภาวะแวดล้อมจะขึ้นอยู่กับประสิทธิภาพของการประเมินความเสี่ยง การวิเคราะห์ข้อมูลที่เกี่ยวข้อง และวิธีในการทดสอบตรวจติดตามสภาวะแวดล้อม อย่างไรก็ตามสิ่งที่สำคัญหลังจากที่จัดทำหรือปรับปรุงโปรแกรมการตรวจติดตามสภาวะแวดล้อมแล้วจะต้องมีการประเมินและปรับปรุงโปรแกรมอย่างต่อเนื่องสม่ำเสมอ เพื่อให้มั่นใจว่าสภาวะแวดล้อมในพื้นที่สะอาดจะคงอยู่ในสถานะที่ควบคุมได้และไม่ส่งผลกระทบต่อคุณภาพของผลิตภัณฑ์
Chemometric Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy Method For Routine Screening Of Paracetamol, Ibuprofen, And Aspirin Adulteration In Herbal Medicines, Mario Theodore
Chulalongkorn University Theses and Dissertations (Chula ETD)
Simple and reliable screening tools for herbal medicine drug adulteration are required, especially in rural areas where complex instrumental methods like HPLC face challenges such as logistics and complex operational processes. Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) is a good identification tool in drug quality control, and its operation is much simpler compared to HPLC, as it requires less sample preparation and has a simple operation. To improve its selectivity in analyzing multicomponent samples like herbal medicine, ATR-FTIR could be combined with chemometric methods such as Linear Discriminant Analysis (LDA). In order to establish itself as a routine testing …
ปัจจัยที่มีผลต่อระยะเวลาการรอดชีวิตของผู้ป่วยมะเร็งเต้านมระยะแพร่กระจายชาวไทย, เพชรรัตน์ ทองดูศรี
ปัจจัยที่มีผลต่อระยะเวลาการรอดชีวิตของผู้ป่วยมะเร็งเต้านมระยะแพร่กระจายชาวไทย, เพชรรัตน์ ทองดูศรี
Chulalongkorn University Theses and Dissertations (Chula ETD)
มะเร็งเต้านมเป็นปัญหาสาธารณสุขที่สำคัญ ผู้ป่วยที่เป็นมะเร็งเต้านมระยะแพร่กระจายเป็นปัจจัยพยากรณ์โรคที่ไม่ดี อย่างไรก็ตาม ยังมีข้อมูลเกี่ยวกับระยะเวลาการรอดชีวิตโดยรวม ปัจจัยที่มีผลต่อระยะเวลาการรอดชีวิต และ อัตราการรอดชีวิตที่ระยะเวลา 5 ปีของผู้ป่วยมะเร็งเต้านมระยะแพร่กระจายชาวไทยอยู่จำกัด การศึกษานี้มีวัตถุประสงค์เพื่อศึกษาปัจจัยที่มีผลต่อระยะเวลาการรอดชีวิตของผู้ป่วยมะเร็งเต้านมระยะแพร่กระจาย โดยเก็บข้อมูลย้อนหลังในผู้ป่วยมะเร็งเต้านมระยะแพร่กระจายที่มารับการรักษาที่โรงพยาบาลศิริราชตั้งแต่วันที่ 1 มกราคม พ.ศ. 2555 ถึง 31 ธันวาคม พ.ศ. 2559 ในช่วงเวลาดังกล่าวมีผู้ป่วยที่มีข้อมูลครบถ้วนและสามารถประเมินข้อมูลระยะเวลาการรอดชีวิตได้จำนวน 790 ราย อายุเฉลี่ย 54+11.8 ปี ส่วนใหญ่ร้อยละ 50.5 เป็นมะเร็งเต้านมชนิด luminal A โรคมะเร็งมีการแพร่กระจายไปที่กระดูกมากที่สุดร้อยละ 70.8 รองลงมาคือที่ปอดและตับ ร้อยละ 55.7 และ 37.3 ตามลำดับ ค่ามัธยฐานของระยะเวลาการรอดชีวิตโดยรวมของผู้ป่วยคือ 21.9 เดือน อัตราการรอดชีวิตที่ 1, 3 และ 5 ปี คือร้อยละ 32.3, 14.4 และ 5.6 ตามลำดับ ผลการวิเคราะห์แบบหลายตัวแปรพบว่าปัจจัยที่มีผลต่อระยะเวลาการรอดชีวิตโดยรวมของผู้ป่วย ได้แก่ ประเภททางโมเลกุลของโรคมะเร็ง ระยะเวลาตั้งแต่ได้รับการวินิจฉัยมากกว่า 6 เดือนขึ้นไปและการแพร่กระจายของโรคมะเร็งไปที่ตับ และการได้รับยาฮอร์โมนหรือยามุ่งเป้า ขณะที่อายุ ดัชนีมวลกาย การได้รับยา chemotherapy หรือสูตรยาที่ได้รับ ไม่มีผลต่อระยะเวลาการรอดชีวิตโดยรวมของผู้ป่วยมะเร็งเต้านมระยะแพร่กระจาย ผู้ป่วยมะเร็งเต้านมระยะแพร่กระจายที่เป็นแบบตัวรับทั้งหมดเป็นบวกหรือแบบไม่มีตัวรับใดเป็นบวก, ได้รับการวินิจฉัยโรคตั้งแต่ 6 เดือนขึ้นไป, มีการแพร่กระจายของโรคมะเร็งไปที่ตับ และผู้ที่ไม่ได้รับยาฮอร์โมนหรือยามุ่งเป้ามีความเสี่ยงในการมีระยะเวลารอดชีวิตโดยรวมที่สั้นกว่า ควรได้รับการวางแผนการรักษาที่เข้มข้นอย่างเหมาะสมเพื่อเพิ่มระยะเวลาการรอดชีวิตโดยรวม
Development Of An Extraction Method Of Mucuna Pruriens Seeds For A Chemically And Physically Stable Extract, Chayarit Vilairat
Development Of An Extraction Method Of Mucuna Pruriens Seeds For A Chemically And Physically Stable Extract, Chayarit Vilairat
Chulalongkorn University Theses and Dissertations (Chula ETD)
Levodopa (L-DOPA) is a drug for the treatment of Parkinson's disease (PD). Currently, it derives from chemical synthesis and plants, especially Mucuna Pruriens (MP) seeds. The results of clinical studies found using the L-DOPA-containing MP for the treatment of PD could reduce side effects more than the synthetic one but patients must take MP powder in high amounts per a single dose which may cause inconvenience. Therefore, using the extract can help in the reduction of the dose. Unfortunately, the water extract from the MP seeds can be easily degraded physically and chemically; especially the physical appearance e.g., darkening color, …
Identification Of Potential Drug Targets For Covid 19 Patients With Cytokine Storm, Bayu Cakra Buana
Identification Of Potential Drug Targets For Covid 19 Patients With Cytokine Storm, Bayu Cakra Buana
Chulalongkorn University Theses and Dissertations (Chula ETD)
The outcome of SARS-CoV-2 infection may vary depending on gender, lifestyle, and age. The elderly is more likely to have the severe condition than the younger population. However, several young people suffer from severe lung diseases caused by cytokine dysregulation, also known as a "cytokine storm." Cytokine storms are associated with Covid-19 severity and mortality. Identification of potential cytokine storm targets is critical for improving patient survival. Transcriptomic analysis of expression data from publicly available databases could be used to identify differentially expressed genes and pathways. The list of both results was used to narrow down the potential candidates in …
Renieramycin T Derivative Dh_22 Induces Apoptosis In Lung Cancer Cells By Downregulation Of C-Myc Protein, Indiana Gita Anggraeni
Renieramycin T Derivative Dh_22 Induces Apoptosis In Lung Cancer Cells By Downregulation Of C-Myc Protein, Indiana Gita Anggraeni
Chulalongkorn University Theses and Dissertations (Chula ETD)
Lung cancer is a prevalent cause of death, indicated by an elevated c-Myc level. We synthesized a new derivative of renieramycin T (RT), named DH_22, and tested it for anti-cancer activities in human lung cancer cells. In the cell viability and nuclear staining tests, DH_22 was discovered to be cytotoxic, with an IC50 of around 13 μM; and found to mediate apoptosis in the lung cancer cells. Meanwhile, in a mechanistic sense, DH_22 contributed to the activation of p53-dependent apoptosis and decreased the cellular level of c-Myc. The p53-dependent mechanism was indicated by an increase of p53, an induction of …
Development Of Plant-Produced Subunit Vaccines To Elicit The Immunogenicity Against Sars-Cov-2 Variant Viruses, Narach Khorattanakulchai
Development Of Plant-Produced Subunit Vaccines To Elicit The Immunogenicity Against Sars-Cov-2 Variant Viruses, Narach Khorattanakulchai
Chulalongkorn University Theses and Dissertations (Chula ETD)
Since the emergence of the coronavirus pandemic in 2019 (COVID-19), the development of effective vaccines to combat the infection has accelerated worldwide. While the severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) with mutations in the receptor-binding domain (RBD) with high transmissibility, enhanced infectivity, and immune escape from vaccination is also predominantly emerging. Effective vaccines against variant of concern (VOC) and optimized booster vaccination strategies are thus highly required. Here, the gene encoding seven different RBD (ancestral (Wuhan), Alpha, Beta, Gamma, Kappa, Delta, and Epsilon variants) fused with the fragment crystallizable region (Fc) of human IgG1 (RBD-Fc) was constructed and cloned into …
Titania Nanosheets Induce Anticancer Activityin Human Non-Small Cell Lung Cancer Cells, Rapeepun Soonnarong
Titania Nanosheets Induce Anticancer Activityin Human Non-Small Cell Lung Cancer Cells, Rapeepun Soonnarong
Chulalongkorn University Theses and Dissertations (Chula ETD)
Metal nanomaterials can enhance the efficacy of current cancer therapies. Here, we show that Ti0.8O2 nanosheets cause cytotoxicity in several lung cancer cells but not in normal cells. The nanosheet-treated cells showed certain apoptosis characteristics. Protein analysis further indicated the activation of the p53-dependent death mechanism. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) analyses revealed the cellular uptake of the nanosheets and the induction of cell morphological change. Ti0.8O2 nanosheets induce apoptosis through a molecular mechanism involving peroxynitrite (ONOO−) generation. As peroxynitrite is known to be a potent inducer of S-nitrosylation, we further found that the nanosheets mediated …
Cholesterol-Lowering And Probiotic Properties Of Selected Lactic Acid Bacteria, Engkarat Kingkaew
Cholesterol-Lowering And Probiotic Properties Of Selected Lactic Acid Bacteria, Engkarat Kingkaew
Chulalongkorn University Theses and Dissertations (Chula ETD)
Ninety strains of lactic acid bacteria (LAB) isolated from fermented foods and bark of Tamarindus indica were screening cholesterol-lowering effects, selected and identified based on the phenotypic, chemotaxonomic characteristics and 16S rRNA gene sequence analysis. They were identified as Companilactobacillus farciminis (5 strains), C. formosensis (10 strains), C. futsaii (6 strains), C. pabuli (4 strains), Enterococcus thailandicus (2 strains), Lactiplantibacillus argentoratensis (3 strains), Lactiplantibacillus pentosus (10 strains), Lactiplantibacillus plantarum subsp. plantarum (17 strains), Lactococcus lactis subsp. lactis (2 strains), Lentilactobacillus buchneri (8 strains), Limosilactobacillus fermentum (2 strains), P. pentosaceus (12 strains) and each of En. durans, En. hirae, En. lactis, …
Antioxidant Activity Of Thunbergia Laurifolia Lindl. Ethanolic Extract In Keratinocytes, Buakotchaphan Jirabanjerdsiri
Antioxidant Activity Of Thunbergia Laurifolia Lindl. Ethanolic Extract In Keratinocytes, Buakotchaphan Jirabanjerdsiri
Chulalongkorn University Theses and Dissertations (Chula ETD)
Fine particulate matter (PM2.5) can cause skin damage through the induction of oxidative stress in epidermis. Antioxidants help counteract with cellular oxidant species and maintain cell homeostasis. This study aims to examine the protective effect of Thunbergia laurifolia lindl. ethanolic extract on PM2.5-mediated oxidative stress in epidermal keratinocytes. High-performance liquid chromatography with diode array detection was conducted for quantitative analysis of rosmarinic acid content in the extract. Cell viability was detected by the 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide assay. Intracellular ROS was monitored using 2’,7’-Dichlorodihydrofluorescein diacetate. Proteins related to p62-Keap1-Nrf2 signaling pathway were examined by western blot analysis. Expressions of Nrf2 and p62 …
Effect Of 5-O-(N-Boc-L-Alanine)-Renieramycin T In The Suppression Of Lung Cancer Stem Cells, Darinthip Suksamai
Effect Of 5-O-(N-Boc-L-Alanine)-Renieramycin T In The Suppression Of Lung Cancer Stem Cells, Darinthip Suksamai
Chulalongkorn University Theses and Dissertations (Chula ETD)
Cancer stem cells (CSCs) drive aggressiveness and metastasis by utilizing stem cell-related signals. In this study, 5-O-(N-Boc-L-alanine)-renieramycin T (OBA-RT) was demonstrated to suppress CSC signals and induce apoptosis. OBA-RT exerted cytotoxic effects with a half-maximal inhibitory concentration of approximately 7 µM and mediated apoptosis as detected by annexin V/PI and nuclear staining assays. Mechanistically, OBA-RT exerted dual roles, activating p53-dependent apoptosis and concomitantly suppressing CSC signals. A p53-dependent pathway was indicated by the induction of p53 and the depletion of anti-apoptotic Mcl-1 and Bcl-2 proteins. Cleaved PARP was detected in OBA-RT-treated cells. Interestingly, OBA-RT exerted strong CSC-suppressing activity, reducing the …
Antioxidant With Antimelanogenic Activities Of Sericin Hydrolysates Optimized By Response Surface Methodology In Human Melanin-Generating Mnt-1 Cells, Keerati Joyjamras
Antioxidant With Antimelanogenic Activities Of Sericin Hydrolysates Optimized By Response Surface Methodology In Human Melanin-Generating Mnt-1 Cells, Keerati Joyjamras
Chulalongkorn University Theses and Dissertations (Chula ETD)
Sericin, a protein presenting in the wastewater from silk industry, causes water pollution and ecological problem. To increase economic value to this waste product, the optimum enzymatic condition that could create sericin hydrolysates with high antioxidant and antimelanogenic capacities was generated through response surface methodology (RSM). Response surface plots demonstrate the major role of temperature on scavenging capacity of sericin hydrolysates assessed via DPPH, FRAP and ORAC assays. Alcalase®-hydrolyzed sericin consisted of sericin-related peptides in differing amounts and smaller sizes compared with unhydrolyzed sericin, as respectively demonstrated by peptidomic and SDS-PAGE analysis. Pre-incubation with 20 mg/mL sericin hydrolysates digested by …
Development Of Mucoadhesive Polymeric Nanoparticles For Intranasal Delivery Of Favipiravir In Treating Sars-Cov-2 Infection, Khent Alcantara
Development Of Mucoadhesive Polymeric Nanoparticles For Intranasal Delivery Of Favipiravir In Treating Sars-Cov-2 Infection, Khent Alcantara
Chulalongkorn University Theses and Dissertations (Chula ETD)
Nanoparticles (NPs) have been used as an effective carrier for transmucosal delivery of drugs through their prolonged residence time and deposition, or the enhanced permeation through the mucosa. In this study, we aimed to design a nanocarrier using the combined mucoadhesive (M) properties of chitosan (CS) and alginate (ALG) polymers for the intranasal delivery of favipiravir (FVR) in treating SARS-CoV-2 infection. The optimized FVR-loaded mucoadhesive CS-coated ALG-NPs (FVR-MCS-ALG-NPs) rendered suitable size (233.5 nm), zeta potential (-21.6 mV), loading capacity (26.0%), and encapsulation efficiency (84.6%) for transmucosal delivery. Superior mucoadhesiveness and higher permeation and deposition in the porcine nasal mucosa of …