Pharmacy and Pharmaceutical Sciences Commons^™

Using Hyperactive Shp-2 Mutants, A Phosphatase, To Identify Interactions With The Rna Helicase Ddx3, Devin Montgomery

Theses and Dissertations--Pharmacy

Protein-protein interactions are critical in cellular signaling pathways. This project investigates binding interactions between SHP-2, a tyrosine phosphatase, and DDX3, an RNA helicase. There is a specific focus on the impact that gain-of-function SHP-2 mutations may impact binding to DDX3 via conformational change. Using immunoprecipitation protocols as well as western blotting we were able to provide insights into SHP-2 molecular mechanisms and subsequent binding to DDX3.

Anxiolytic Intervention: Modulation Of Cell Types Due To Nicotine Withdrawal, Taylor Elder

Theses and Dissertations--Pharmacy

Nicotine use disorder (NUD) remains a leading cause of preventable disease, yet the neurobiological mechanisms underlying nicotine withdrawal are incompletely understood. Emerging evidence implicates neuroimmune signaling and astrocytic regulation of glutamate in shaping the withdrawal-associated affective phenotype. This dissertation investigated these processes using multiple nicotine administration paradigms including osmotic minipump implant, cigarette smoke exposure, and nicotine vapor inhalation to model diverse routes of human nicotine intake.

Chapter 2 examines the effects of these three modes of administration on neuroinflammation and validation of nicotine delivery. Nicotine exposure was confirmed through receptor [³H]Epibatidine nAChR binding assay and serum cotinine measurements. …

Investigating The Role Of Dynamic Signaling Of Nrg3-Erbb4 In The Prefrontal Cortex In Mediating Nicotine Withdrawal Phenotypes, Emily Prantzalos

Theses and Dissertations--Pharmacy

While several pharmacotherapies are currently available for nicotine use disorder (NUD), smoking cessation success rates remain below 10% each year. The molecular mechanisms underlying failed quit attempts continue to represent a critical gap in our knowledge. However, recent advances in understanding the genetic contributions to nicotine dependence offer a promising avenue for developing more effective cessation therapies. Prior research has identified genetic variation in Neuregulin 3 (Nrg3) and its cognate receptor ErbB4 as potential contributors to affective nicotine withdrawal (WD) phenotypes and cessation outcomes. Therefore, this dissertation aims to systematically investigate the region-specific, functional role of the Neuregulin Signaling Pathway …

Leveraging Transcriptomics And Behavior To Investigate Potential Neuronal And Glial Drivers Of Substance Use Disorder Endophenotypes, Jack V. Keady

Theses and Dissertations--Pharmacy

Over 54 million Americans needed substance use treatment in 2023. While there are many facets of substance use disorder, two major issues of interest are the negative reinforcing aspects of substances of abuse, manifesting in part as withdrawal symptomologies, and why a subset of people who use substances of abuse escalate to a use disorder. Nicotine exposure models were used to investigate the mechanisms of withdrawal endophenotypes, as smoking is the leading cause of preventable death in the United States, and a fentanyl self-administration model was used to probe individual differences in escalation phenotypes, as 80% of overdose deaths in …

The Impact Of Maternal Environmental Exposures On The Immune Landscape Of The Maternal-Fetal Interface, Heather E. True

Theses and Dissertations--Pharmacy

Maternal environmental exposures and inflammation are known to impact fetal development in utero as well as increase the risk for chronic diseases later in life. Therefore, it is essential that we understand how the maternal environment alters placental structure and immune function.

Therefore, we isolated fetal (chorionic villous) tissues from the placentas of healthy pregnant participants (control) and those with inflammatory conditions, including SARS-COV-2 infection, pregravid obesity, and opioid use disorder. Isolated placental leukocytes were subjected to a series of assays to uncover the phenotypic, functional, and transcriptional (single-cell RNA-sequencing) landscape of placental immune cells. Additional placental tissues were subjected …

Physicochemical Property Effects On Immune Modulating Polymeric Nanoparticles: Potential Applications In Spinal Cord Injury, Daniel J. Kolpek, Jaechang Kim, Hisham Mohammed, John C. Gensel, Jonghyuck Park

Markey Cancer Center Faculty Publications

Nanoparticles (NPs) offer promising potential as therapeutic agents for inflammation-related diseases, owing to their capabilities in drug delivery and immune modulation. In preclinical studies focusing on spinal cord injury (SCI), polymeric NPs have demonstrated the ability to reprogram innate immune cells. This reprogramming results in redirecting immune cells away from the injury site, downregulating pro-inflammatory signaling, and promoting a regenerative environment post-injury. However, to fully understand the mechanisms driving these effects and maximize therapeutic efficacy, it is crucial to assess NP interactions with innate immune cells. This review examines how the physicochemical properties of polymeric NPs influence their modulation of …

Table B: Impacts Of Utensil Use In Preparation, Robert Yokel

Aluminum in Beverages and Foods: A Compilation

No abstract provided.

Coffee Beans, Robert Yokel

Aluminum in Beverages and Foods: A Compilation

No abstract provided.

Creamer, Non-Dairy, Robert Yokel

Aluminum in Beverages and Foods: A Compilation

No abstract provided.

Tea Infusion, Unspecified Tea Type, Robert Yokel

Aluminum in Beverages and Foods: A Compilation

No abstract provided.

Puer Tea Infusion, Robert Yokel

Aluminum in Beverages and Foods: A Compilation

No abstract provided.

Energy, Isotonic, Sports, And Nutritional Drinks, Robert Yokel

Aluminum in Beverages and Foods: A Compilation

No abstract provided.

Butter, Robert Yokel

Aluminum in Beverages and Foods: A Compilation

No abstract provided.

Sugar, Syrup And Molasses, Robert Yokel

Aluminum in Beverages and Foods: A Compilation

No abstract provided.

Oils, Fats, And Their Products, Robert Yokel

Aluminum in Beverages and Foods: A Compilation

No abstract provided.

Pepper, Robert Yokel

Aluminum in Beverages and Foods: A Compilation

No abstract provided.

Rice, Robert Yokel

Aluminum in Beverages and Foods: A Compilation

No abstract provided.

Cookies And Snacks, Robert Yokel

Aluminum in Beverages and Foods: A Compilation

No abstract provided.

Barley, Robert Yokel

Aluminum in Beverages and Foods: A Compilation

No abstract provided.

Mango And Mango Juice, Robert Yokel

Aluminum in Beverages and Foods: A Compilation

No abstract provided.

Cherries And Cherry Juices, Robert Yokel

Aluminum in Beverages and Foods: A Compilation

No abstract provided.

Table I: Aluminum Material Age Influence On Aluminum Mobilization Into Beverages And Foods, Robert Yokel

Aluminum in Beverages and Foods: A Compilation

No abstract provided.

Table C: Ph Influence On Aluminum Mobilization Into Beverages And Foods, Robert Yokel

Aluminum in Beverages and Foods: A Compilation

No abstract provided.

Potato, Robert Yokel

Aluminum in Beverages and Foods: A Compilation

No abstract provided.

Cauliflower, Robert Yokel

Aluminum in Beverages and Foods: A Compilation

No abstract provided.

Carrot And Parsnip, Robert Yokel

Aluminum in Beverages and Foods: A Compilation

No abstract provided.

Processed Cheese, Robert Yokel

Aluminum in Beverages and Foods: A Compilation

No abstract provided.

Jellyfish, Robert Yokel

Aluminum in Beverages and Foods: A Compilation

No abstract provided.

Seaweeds, Robert Yokel

Aluminum in Beverages and Foods: A Compilation

No abstract provided.

Plums, Prunes And Their Juices, Robert Yokel

Aluminum in Beverages and Foods: A Compilation

No abstract provided.