Pharmacy and Pharmaceutical Sciences Commons^™

Formulation And Evaluation Of Fast Disintegrating Sublingual Atropine Tablets For The Potential Treatment Of Acute Organophosphates Toxicity, Alhussain Aodah

Student Theses, Dissertations and Capstones

Organophosphates (OP) are chemicals found in certain pesticides and in nerve agents such as sarin. Toxicity with these agents can lead to death. On August 2013, 1,300 civilians were killed using sarin gas in Syria by the Assad regimen. The lack of prepared medical facilities, and the availability of appropriate treatmentsignificantly contributed to the high percent of causality. Further, worldwide pesticides toxicity affects 3 million people annually. Therefore, it is imperative that antidote to treat OP toxicity are readily available in a dosage form that is acceptable cost, effective, and user friendly. Atropine sulfate (AS) is the first-line antidote forOP …

Pathophysiological Relevance Of Astroglial Angiotensin And The Endocannabinoid Signaling Systems In Shrs, Dhanush Haspula

Student Theses, Dissertations and Capstones

BACKGROUND: Spontaneously hypertensive rats (SHRs), an essential hypertension model, are characterized by pro-inflammatory states in brainstems. While Angiotensin (Ang) II, via activation of glial Ang Type 1 receptors (AT1Rs), has been shown to trigger a significant upsurge in pro-inflammatory cytokines, activation of astroglial Cannabinoid Type 1 Receptors (CB1Rs), elicits a potent anti-inflammatory response in the brain. Both brainstem AT1Rs and CB1Rs have also been reported to alter cardiovascular parameters in SHRs. Evidence of crosstalk between CB1Rs and AT1Rs has also emerged, further highlighting a need to understand their signaling interaction in cardiovascular diseases. APPROACH: The purpose of this study was …

Measuring Knowledge And Attitudes Regarding The Use Of Pharmacogenetic Testing Among Patients And Prescribers: Diffusion Of Innovation Theory, Suhaib Mohammad Muflih

Student Theses, Dissertations and Capstones

Background: Healthcare providers play a key role in patient care. Their knowledge and attitudes may play a critical role in the incorporation of pharmacogenetic (PGx) testing into routine practice. The knowledge and attitudes of patients are also equally important in determining the rate of diffusion and the adoption of PGx testing. This study aims to test Rogers’s diffusion of innovation theory to identify and evaluate the influence of knowledge, attitudes, and sociodemographic characteristics of patients and physicians on the adoption of PGx testing in current clinical settings. Method: A cross-sectional, descriptive survey design was implemented. The sample consisted of patients …

Nsu Sharks Rx-Winter, 2017, Nova Southeastern University

NSU Sharks Rx

No abstract provided.

Nsu Sharks Rx - Fall 2016, Nova Southeastern University

NSU Sharks Rx

No abstract provided.

Renin-Angiotensin System Gene Expression And Neurodegenerative Diseases., Benjamin Goldstein, Robert Charles Speth, Malav Suchin Trivedi

Faculty Articles

HYPOTHESIS: Single nucleotide polymorphisms and altered gene expression of components of the renin-angiotensin system (RAS) are associated with neurodegenerative diseases.

INTRODUCTION: Drugs that interact with the RAS have been shown to affect the course of neurodegenerative disease, suggesting that abnormalities in the RAS may contribute to neurodegenerative disease.

MATERIALS AND METHODS: A meta-analysis of genome-wide association studies and gene expression data for 14 RAS-related proteins was carried out for five neurodegenerative diseases: Alzheimer's disease, Parkinson's disease, narcolepsy, amyotrophic lateral sclerosis and multiple sclerosis.

RESULTS: No single nucleotide polymorphisms in any of the 14 RAS-related protein genes were significantly associated with …

Nsu Sharks Rx-Summer, 2016, Nova Southeastern University

NSU Sharks Rx

No abstract provided.

An In Vitro And In Vivo Investigation Of Bivalent Ligands That Display Preferential Binding And Functional Activity For Different Melanocortin Receptor Homodimers, Cody J Lensing, Katie T Freeman, Sathya M Schnell, Danielle N Adank, Robert Charles Speth, Carrie Haskell-Luevano

Faculty Articles

Pharmacological probes for the melanocortin receptors have been utilized for studying various disease states including cancer, sexual function disorders, Alzheimer's disease, social disorders, cachexia, and obesity. This study focused on the design and synthesis of bivalent ligands to target melanocortin receptor homodimers. Lead ligands increased binding affinity by 14- to 25-fold and increased cAMP signaling potency by 3- to 5-fold compared to their monovalent counterparts. Unexpectedly, different bivalent ligands showed preferences for particular melanocortin receptor subtypes depending on the linker that connected the binding scaffolds, suggesting structural differences between the various dimer subtypes. Homobivalent compound 12 possessed a functional profile …

Nsu Sharks Rx-Spring, 2016, College Of Pharmacy

NSU Sharks Rx

Volume 2, Number 1

Nsu Sharks Rx-Spring, 2016, Nova Southeastern University

NSU Sharks Rx

No abstract provided.

A 5'-Upstream Short Open Reading Frame Encoded Peptide Regulates Angiotensin Type 1a Receptor Production And Signalling Via The Β-Arrestin Pathway, Gina L. Yosten, Jun Liu, Hong Ji, Kathryn Sandberg, Robert Speth, Willis K. Samson

HPD Articles

AUG sequences and short open reading frames are commonly present in the 5'-leader sequence of G protein-coupled receptor mRNAs. The presence of these upstream AUG sequences has been demonstrated to inhibit downstream receptor translation efficiency and, most recently, receptor signal transduction. A seven amino acid peptide encoded by a short open reading frame in exon 2 of the angiotensin type 1a receptor has been shown to inhibit non-G protein-coupled signalling of angiotensin II, without altering the classical G protein-coupled pathway activated by the ligand. This finding may lead to the development of a new class of angiotensin receptor antagonists with …

Doctor Of Pharmacy-Philosophy Of Pharmacy 2016, Nova Southeastern University

Health Professions Divisions Course Catalogs and Course Descriptions

No abstract provided.

Doctor Of Pharmacy Curriculum, Nova Southeastern University

Health Professions Divisions Course Catalogs and Course Descriptions

No abstract provided.

Doctor Of Pharmacy Overview, Nova Southeastern University

Health Professions Divisions Course Catalogs and Course Descriptions

No abstract provided.

Evaluation Of Current Heparin Weight Based Protocol In Obese Patients, Marie Lafosse, Simon Leung, Carla Hawkins, Madeline Camejo

Internet Journal of Allied Health Sciences and Practice

Purpose: To evaluate the difference in achieving goal activated partial thromboplastin time (aPTT) within 24 hours utilizing the institutional heparin weight based protocol between obese and non-obese patients. Methods: Retrospective, non-randomized, open label chart review in a community based hospital. Patients age 18 years or older receiving heparin therapy for greater than or equal to 24 hours identified. Patients were excluded if the protocol was utilized for acute coronary syndrome or interrupted within the initial 24 hours of therapy. Patients were also excluded if any deviations from the protocol were identified. The primary endpoint evaluated the difference in achieving goal …

Selective C1 Lesioning Slightly Decreases Angiotensin Ii Type I Receptor Expression In The Rat Rostral Ventrolateral Medulla (Rvlm)., Erick A Bourassa, Kristen A Stedenfeld, Alan F Sved, Robert Charles Speth

Faculty Articles

Cardiovascular homeostasis is regulated in large part by the rostral ventrolateral medulla (RVLM) in mammals. Projections from the RVLM to the intermediolateral column of the thoracolumbar spinal cord innervate preganglionic neurons of the sympathetic nervous system causing elevation of blood pressure and heart rate. A large proportion, but not all, of the neurons in the RVLM contain the enzymes necessary for the production of epinephrine and are identified as the C1 cell group. Angiotensin II (Ang II) activates the RVLM acting upon AT1 receptors. To assess the proportion of AT1 receptors that are located on C1 neurons in the rat …

Nsu Sharks Rx - Fall 2015, College Of Pharmacy

NSU Sharks Rx

No abstract provided.

Advancements In Treatment For Sensorineural Hearing Loss: Implications For Rehabilitation Professionals, Shawn P. Saladin, Yasar Tasnif, Bianca Cruz, Robert B. Perez

JADARA

Rehabilitation professionals often work with individuals with sensorineural hearing loss. Sometimes the hearing loss is due to ototoxic medications that are prescribed as treatments for other conditions. An understanding of the types of ototoxic medications at the root of the sensorineural hearing loss combined with an understanding of the advancements in treatments will help the rehabilitation professional better serve consumers who fit this description.

An Information Privacy Examination Of The Practices Of Pharmaceutical Companies Regarding Use Of Information Collected Through Their Websites, Shonda Dellena Brown

CCAC Theses and Dissertations

Consumers have begun to take a more proactive approach to their healthcare by accessing pharmaceutical companies Websites to obtain health and drug information, support groups, rebates, coupons, as well as free drug trials. In exchange for these benefits, companies require consumers to voluntarily disclose information. However, research has shown that consumers continue to be concerned about how their information is managed, used, and distributed by companies, especially if accessed via the Web. To date, there has been limited empirical research to examine the actual online practices of companies when it comes to privacy, especially those of pharmaceutical companies. Using Delphi …

Nsu Sharks Rx - Spring 2015, College Of Pharmacy

NSU Sharks Rx

No abstract provided.

Health Professions Division Catalog 2015-2016, Nova Southeastern University

Health Professions Divisions Course Catalogs and Course Descriptions

No abstract provided.

Doctor Of Pharmacy-Philosophy Of Pharmacy 2015, Nova Southeastern University

Health Professions Divisions Course Catalogs and Course Descriptions

No abstract provided.

Acute Repeated Intracerebroventricular Injections Of Angiotensin Ii Reduce Agonist And Antagonist Radioligand Binding In The Paraventricular Nucleus Of The Hypothalamus And Median Preoptic Nucleus In The Rat Brain, Robert C. Speth, Peter J. Vento, Eduardo J. Carrera, Luz Gonzalez-Reily, Andrea Linares, Kira Santos, Jamala D. Swindle, Derek Daniels

HPD Articles

Angiotensin II (Ang II) stimulates water and saline intakes when injected into the brain of rats. This arises from activation of the AT1 Ang II receptor subtype. Acute repeated injections, however, decrease the water intake response to Ang II without affecting saline intake. Previous studies provide evidence that Ang II-induced water intake is mediated via the classical G protein coupling pathway, whereas the saline intake caused by Ang II is mediated by an ERK 1/2 MAP kinase signaling pathway. Accordingly, the different behavioral response to repeated injections of Ang II may reflect a selective effect on G protein coupling. To …

Increased Expression Of Angiotensin Ii Type 2 Receptors In The Solitary-Vagal Complex Blunts Renovascular Hypertension, Graziela Torres Blanch, André Henrique Freiria-Oliveira, Guilherme Fleury Speretta, Eduardo J. Carrera, Hongwei Li, Robert C. Speth, Eduardo Colombari, Colin Sumners, Débora S. Colombari

HPD Articles

Angiotensin II increases and decreases arterial pressure by acting at angiotensin type 1 and type 2 receptors, respectively. Renovascular hypertensive rats exhibit a high level of activity of the peripheral and central renin-angiotensin system. Therefore, in the present study, we evaluated the effect of increasing the expression of angiotensin type 2 receptors in the solitary-vagal complex (nucleus of the solitary tract/dorsal motor nucleus of the vagus), a key brain stem region for cardiovascular regulation, on the development of renovascular hypertension. Holtzman normotensive rats were implanted with a silver clip around the left renal artery to induce 2-kidney 1-clip renovascular hypertension. …

Selective Inhibition Of Angiotensin Receptor Signaling Through Erk1/2 Pathway By A Novel Peptide, Jun Liu, Gina L. Yosten, Hong Ji, Dan Zhang, Wei Zheng, Robert C. Speth, Willis K. Samson, Kathryn Sandberg

HPD Articles

A seven-amino acid peptide (PEP7) is encoded within a short open reading frame within exon 2 (E2) in the 5'-leader sequence (5'LS) upstream of the rat ANG 1a-receptor (rAT1aR) mRNA. A chemically synthesized PEP7 markedly inhibited ANG II-induced Erk1/2 activation in cell culture by 62% compared with a scrambled PEP7 (sPEP7) [pErk1/2/Erk1/2 (AU): ANG II, 1.000 ± 0.0, ANG II+PEP7, 0.3812 ± 0.086, ANG II+sPEP7, 1.069 ± 0.18; n = 3]. Under these same conditions, PEP7 had no effect on ANG II-stimulated inositol-trisphosphate production. PEP7 also had no effect on epidermal growth factor- and phorbol methyl ester-induced Erk1/2 activation, suggesting …

The Fda Funding Crisis, Judith Alphonse, Sireesha Bellam, Marlene Fernandez, Anishka Gilbert, Lauren Roper, Antonia Zapantis, Robert C. Speth

HPD Articles

The role of the Food and Drug Administration (FDA) is to ensure the safety of prescription and nonprescription drugs, dietary supplements, and the food supply, representing more than 20% of US consumer spending. The increased need to monitor imported drugs, drug products and foods, drug shortages, and compounding pharmacies bring the adequacy of FDA funding into question. Performing even at status quo cannot be accomplished if responsibilities increase without equitable funding increases: both from the federal government and fees imposed on FDA-regulated industries. Additionally, scientific advancement, new legislation, and new industries are continually increasing the FDA workload, necessitating commensurate budget …

Atypical Signaling And Functional Desensitization Response Of Mas Receptor To Peptide Ligands, Kalyan C. Tirupula, Russell Desnoyer, Robert C. Speth, Sadashiva S. Karnik

HPD Articles

MAS is a G protein-coupled receptor (GPCR) implicated in multiple physiological processes. Several physiological peptide ligands such as angiotensin-(1-7), angiotensin fragments and neuropeptide FF (NPFF) are reported to act on MAS. Studies of conventional G protein signaling and receptor desensitization upon stimulation of MAS with the peptide ligands are limited so far. Therefore, we systematically analyzed G protein signals activated by the peptide ligands. MAS-selective non-peptide ligands that were previously shown to activate G proteins were used as controls for comparison on a common cell based assay platform. Activation of MAS by the non-peptide agonist (1) increased intracellular calcium and …

Distribution Of Non-At1, Non-At2 Binding Of 125i-Sarcosine1, Isoleucine8 Angiotensin Ii In Neurolysin Knockout Mouse Brains, Robert C. Speth, Eduardo J. Carrera, Catalina Bretón, Andrea Linares, Luz Gonzalez-Reiley, Jamala D. Swindle, Kira L. Santos, Ines Schadock, Michael Bader, Vardan T. Karamyan

HPD Articles

The recent identification of a novel binding site for angiotensin (Ang) II as the peptidase neurolysin (E.C. 3.4.24.16) has implications for the renin-angiotensin system (RAS). This report describes the distribution of specific binding of 125I-Sarcosine1, Isoleucine8 Ang II (125I-SI Ang II) in neurolysin knockout mouse brains compared to wild-type mouse brains using quantitative receptor autoradiography. In the presence of p-chloromercuribenzoic acid (PCMB), which unmasks the novel binding site, widespread distribution of specific (3 µM Ang II displaceable) 125I-SI Ang II binding in 32 mouse brain regions was observed. Highest levels of binding >700 fmol/g initial wet weight were seen in …

Pharmacological Characterization Of A Novel Non-At1, Non-At2 Angiotensin Binding Site Identified As Neurolysin, Jamala D. Swindle, Kira L. Santos, Robert C. Speth

HPD Articles

The discovery of a novel non-AT1, non-AT2 binding site for angiotensins in the rodent brain and testis that is unmasked by the organomercurial compound para-chloromercuribenzoic acid (PCMB) has catalyzed efforts to purify and characterize this protein. We recently reported that this protein is neurolysin and now report upon the specificity of this binding site for various neuropeptides. Competition binding assays in rat brain and testis used (125)I-Sar(1), Ile(8) angiotensin II (Ang II) as the radioligand in the presence of saturating concentrations of AT1 and AT2 receptor antagonists and 100 μM parachloromercuribenzoate. Primary screening of 36 peptides and other compounds at …

The Effects Of Para-Chloromercuribenzoic Acid And Different Oxidative And Sulfhydryl Agents On A Novel, Non-At1, Non-At2 Angiotensin Binding Site Identified As Neurolysin, Kira L. Santos, Megan A. Vento, John W. Wright, Robert C. Speth

HPD Articles

A novel, non-AT1, non-AT2 brain binding site for angiotensin peptides that is unmasked by p-chloromercuribenzoate (PCMB) has been identified as a membrane associated variant of neurolysin. The ability of different organic and inorganic oxidative and sulfhydryl reactive agents to unmask or inhibit 125I-Sar1Ile8 angiotensin II (SI-Ang II) binding to this site was presently examined. In tissue membranes from homogenates of rat brain and testis incubated in assay buffer containing losartan (10 μM) and PD123319 (10 μM) plus 100 μM PCMB, 5 of the 39 compounds tested inhibited 125I-SI Ang II binding in brain and testis. Mersalyl acid, mercuric chloride (HgCl2) …