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Full-Text Articles in Viruses
Developing Coexpression Systems To Introduce Hydroxyproline Into Protein Engineered Collagen Peptides Utilizing Hydroxylase From Acanthamoeba Polyphaga Mimivirus, Jennifer Soldatich
Developing Coexpression Systems To Introduce Hydroxyproline Into Protein Engineered Collagen Peptides Utilizing Hydroxylase From Acanthamoeba Polyphaga Mimivirus, Jennifer Soldatich
Theses and Dissertations
A major challenge of developing collagen mimetic peptides (CMPs) by bacterial expression is to include hydroxyproline for biomedical applications. Coexpression of Prolyl-4-hydroxylase from A.mimivirus with CMPs was investigated. Additionally, four expression designs were created for potential applications in mammalian cells to utilize their natural ability of proline-hydroxylation by post-translational modification.
Analysis Of Single Cell Rna-Seq Data Revealed Interferon Gamma Signaling Alteration In Severe Covid Patients, Ahmed Elbaz
Analysis Of Single Cell Rna-Seq Data Revealed Interferon Gamma Signaling Alteration In Severe Covid Patients, Ahmed Elbaz
Theses and Dissertations
Background: SARS-COV2 virus detected in December 2019, and was considered a pandemic in March 2020 by the WHO. Symptoms range from asymptomatic to life threatening ones. Studying cell-cell interactions in patients' blood samples may lead to novel diagnosis and treatment approaches.
Aim: This study aims to analyze single-cell RNA sequencing data to identify differences in cell-cell communications between healthy and COVID patients and differentially expressed T-cells genes that contributed to immune system antiviral activity.
Materials and methods: Single-Cell RNA sequencing data from seven COVID patients and five healthy individuals were collected from (GEO accession GSE155673). Cell types were identified and …
The Roles Of Neutralizing Antibodies In Blocking Human Cytomegalovirus Entry Into Multiple Cell Types, Li He
Theses and Dissertations
Human cytomegalovirus (HCMV) can be transmitted through body fluids and cause serious disease in the immunologically immature or immunocompromised population. HCMV entry utilizes different mechanisms involving various viral envelope glycoproteins. gB and gH/gL/gO (trimer) are involved in fibroblast entry and gH/gL/U128-131A (pentamer) is required in addition to trimer for epithelial and endothelial cell entry. gO is heavily glycosylated and highly polymorphic in different HCMV strains. gO in the parental TB40/E strain was completely or partially replaced by gOs from other HCMV strains. A quantitative luciferase-based assay demonstrated that certain polymorphic gOs from different strains can interfere with neutralization of fibroblast …