Viruses Commons^™

The Structure Of A T=169d Algal Virus, Pbcv-1, At 15Å Resolution, X. Yan, V. Bowman, N. H. Olson, James Gurnon, James L. Van Etten, Michael G. Rossmann, T. S. Baker

James Van Etten Publications

Paramecium bursaria chlorella virus 1 (PBCV-1) (genus Chlorovirus, family Phycodnaviridae), infects certain unicellular, exsymbiotic, chlorella-like green algae [1]. The PBCV-1 virion (~1x109 Da) has a linear dsDNA genome (330kbp) and contains at least 100 different proteins [1, unpublished data]. A published cryo-electron microscopy and three-dimensional (3D) image reconstruction of PBCV-1 (26Å resolution) revealed that the outer icosahedral glycoprotein capsid, with a maximum diameter of 1,900Å along the five-fold axis, consists of 1680 trimeric capsomers (trimer) and 12 pentameric capsomers (pentamer) [2]. These capsomers are arranged on a T=169d (h=7, k=8) quasi-equivalent lattice. The trimers and pentamers are organized into 20 …

Functional Implication Of The Trna Genes Encoded In The Chlorella Virus Pbcv-L Genome, Da Young Lee, Michael V. Graves, James L. Van Etten, Tae-Jin Choi

James Van Etten Publications

The prototype Chlorella virus PBCV-l encodes 11 tRNA genes and over 350 protein-encoding genes in its 330 kbp genome. Initial attempts to overexpress the recombinant A189/192R protein, a putative virus attachment protein, in E. coli strain BL21(DE3) SI were unsuccessful, and multiple protein bands were detected on Western blots. However, the full-length A189/192R recombinant protein or fragments derived from it were detected when they were expressed in E. coli BL21 CodonPlus (DE3) RIL, which contains extra tRNAs. Codon usage analysis of the a1891192r gene showed highly biased usage of the AGA and AUA codons compared to genes encoded by E. …

Glycosyltransferases Encoded By Viruses, Nicolas Markine-Goriaynoff, Laurent Gillet, James L. Van Etten, Haralambos Korres, Naresh Verma, Alain Vanderplasschen

James Van Etten Publications

Studies of cellular biology in recent decades have highlighted the crucial roles of glycans in numerous important biological processes, raising the concept of glycomics that is now considered as important as genomics, transcriptomics and proteomics. For millions of years, viruses have been co-evolving with their hosts. Consequently, during this co-evolution process, viruses have acquired mechanisms to mimic, hijack or sabotage host processes that favour their replication, including mechanisms to modify the glycome. The importance of the glycome in the regulation of host–virus interactions has recently led to a new concept called ‘glycovirology’. One fascinating aspect of glycovirology is the study …

Can Large Dsdna-Containing Viruses Provide Information About The Minimal Genome Size Required To Support Life?, James L. Van Etten

James Van Etten Publications

The genomes of a few viruses, such as Bacillus megaterium phage G (670 kb) and the chlorella viruses (330 to 380 kb), are larger than the predicted minimal genome size required to support life (ca. 320 kb). A comparison of the 256 proteins predicted to be required for life with the putative 376 proteins encoded by chlorella virus PBCV-1, as well as those encoded by other large viruses, indicates that viruses lack many of these “essential” genes. Consequently, it is unlikely that viruses will aid in determining the minimal number and types of genes required for life. However, viruses may …

Infectious Pancreatic Necrosis (Ipn) Of Salmonid Fishes, Phillip E. Mcallister

United States Fish and Wildlife Service: Publications

Infectious pancreatic necrosis (IPN) is a viral disease principally associated with salmonids, although IPN and lPN-like viruses have been isolated from various nonsa1rnonid fish and marine invertebrates. Acute infection occurs in 1- to 4-month-old salmonids and can result in cumulative mortality approaching 100%. In contrast, fish 6 months old or older can undergo subclinical or inapparent infection, but experience no significant mortality. Survivors of the disease can become lifelong carriers of the virus. Epizootics of IPN have been reported worldwide, and several virus serotypes are recognized. The disease known as IPN was probably first described by M'Gonigle (1941) as acute …

Plant Viruses., G. D. Mclean, L. K. Price, J. A. Weir, J. M. Allen, P. Fievez, P. A. Portmann, R. J. Mclean, J. D. Sandow

Experimental Summaries - Plant Research

Clover viruses, 82ES38, 82AL47, 82MA19, 82BR19, 82BY29; 82BU5, 82HA9. Lupin virus, diseases. Barley yellow dwarf virus, 82AL46, 82AL51, 82B10, 82BA33, 82BR16, 82BR18, 82C29, 82E27, 82ES37, 82ES40, 82JE19, 82JE20, 82KA33, 82KA34, 82ABI3, 82MA18, 82MN22, 82MT34, 82NA32, 82WH28,82B8, 82MN17, 82E24, 82MT30, 82E25, 82MN18, 82MT31, 82B9, 82ABI2, 82BA31, 82C26, 82JE17, 82WH27, 82AL45, 82BR17, 82ES39, 82MA1, 82MA117, 82MT33,

Isolation And Enumeration Of Enteric Viruses From Primary Wastewater Sludge, Michael Wayne Nath

Biological Sciences Theses & Dissertations

The presence of large numbers of enteric viruses in wastewaters has resulted in concern regarding their significance and consequences for humans. Assessments of relative significance or consequences may most accurately be made following precise measurements of the range of virus types and amounts in wastewaters and sludges and after epidemiological considerations. In this study, results are presented pertaining to enteric virus isolation, concentration, and enumeration from primary wastewater sludges.

As an integral part of a comparison of isolation procedures, several anionic surfactants were examined for extraction of enteric viruses from primary wastewater sludges. Plaquing efficiencies for poliovirus type 2 and …

Oral History Interview: Edward C. Jackson, Edward C. Jackson

0064: Marshall University Oral History Collection

Edward C. Jackson was born on January 17, 1911, in Canfield, Braxton County, WV. In 1926, he moved to Fenwick, Nicholas County, WV, to pursue jobs in saw mills. In his interview, Mr. Jackson describes in great detail the design and structure of the log home he lived in as a child. He focuses on growing up on a farm and places emphasis on the type of work he did and food preparation and preservation. Mr. Jackson also discusses the tight-knit community in which he was raised. In the audio clip provided, Mr. Jackson discusses working in the lumber industry. …

Comparison Of Interferon Produced In Vitro By Monkey Kidney Cells Demonstrating Interference Against Polio Ii, Coxsackie A-9, Influenza A2/Hong Kong/68, And Echo-9 Viruses, Archer Duncan Farmer Jr.

Biological Sciences Theses & Dissertations

Abstract unavailable

Experimentation To Develop Procedures To Be Used In The Investigation Of The Effects Of Restriction On The Unmodified Dna Of The Bacteriophage Lamda, John Haynes

Honors Theses

The results of experimentation approximately twenty years ago with variuos bacteriophages by Ralston and Krueger¹⁶, Anderson and Felix¹, Luria and Human¹², and by Bertani and Weigle² have shown that the host range of a given phage depends directly on the bacterial strain on which the phage has last propagated. This event came to be known as host-induced modification or host-controlled variation. As this phage host range is controlled by the host environment and can be altered from one generation to another it has, according to Luria, made microbiology "the last stronghold of Lamarckism" …