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Articles 1 - 3 of 3
Full-Text Articles in Organisms
One-Step Hot Formamide Extraction Of Rna From Saccharomyces Cerevisiae, Daniel Shedlovskiy, Natalia Shcherbik, Dimitri G Pestov
One-Step Hot Formamide Extraction Of Rna From Saccharomyces Cerevisiae, Daniel Shedlovskiy, Natalia Shcherbik, Dimitri G Pestov
Rowan-Virtua School of Osteopathic Medicine Faculty Scholarship
Current methods for isolating RNA from budding yeast require lengthy and laborious steps such as freezing and heating with phenol, homogenization with glass beads, or enzymatic digestion of the cell wall. Here, extraction with a solution of formamide and EDTA was adapted to isolate RNA from whole yeast cells through a rapid and easily scalable procedure that does not require mechanical cell lysis, phenol, or enzymes. RNA extracted with formamide-EDTA can be directly loaded on gels for electrophoretic analysis without alcohol precipitation. A simplified protocol for downstream DNase treatment and reverse transcription reaction is also included. The formamide-EDTA extraction of …
Environmental Change Alters Personality In The Rainbow Trout, Oncorhynchus Mykiss, Ashley J. Frost, Jack S. Thomson, Charlotte Smith, Hannah C. Burton, Ben Davis, Phillip C. Watts, Lynne U. Sneddon
Environmental Change Alters Personality In The Rainbow Trout, Oncorhynchus Mykiss, Ashley J. Frost, Jack S. Thomson, Charlotte Smith, Hannah C. Burton, Ben Davis, Phillip C. Watts, Lynne U. Sneddon
Lynne Sneddon, PhD
Boldness is a personality trait that defines how individuals respond to risky situations and has clear fitness consequences. Since the adaptive value of boldness is context dependent, the benefit of a distinct personality is less clear when the environment is unpredictable. An ability to modulate behaviour can be beneficial, although as behavioural plasticity itself may be costly this depends on the levels of environmental stability. Both boldness and its plasticity are linked with physiological stress coping mechanisms, whereby animals with reduced glucocorticoid responses to stress are bolder and less flexible in behaviour. We investigated the behavioural changes made by bold …
Hovering Energetics And Thermal Balance In Anna's Hummingbirds (Calypte Anna), Dennis Evangelista, Maria J. Fernandez, Madalyn S. Berns, Aaron M. Hoover, Robert Dudley
Hovering Energetics And Thermal Balance In Anna's Hummingbirds (Calypte Anna), Dennis Evangelista, Maria J. Fernandez, Madalyn S. Berns, Aaron M. Hoover, Robert Dudley
Aaron M. Hoover
We studied the energetics of hover-feeding Anna's hummingbirds, using three different simultaneous techniques: heat loss as estimated via thermal imaging, metabolic rate as measured at a feeder mask using flow-through respirometry, and aerodynamic power estimated from wingbeat kinematic data. These three methods yielded comparable estimates of power output at ambient air temperatures ranging from 18 degrees to 26 degrees C, whereas heat imbalance at higher air temperatures (up to 34 degrees C) suggested loss by mechanisms other than convection and radiation from the body, such as evaporative cooling and enthalpy rise associated with exhaled air and excreted water and convective …