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Full-Text Articles in Organisms
Site Tolerance Of The Rada Intein, Chase Taul, Christopher Lennon Dr
Site Tolerance Of The Rada Intein, Chase Taul, Christopher Lennon Dr
Honors College Theses
Inteins (intervening proteins) invade genes at the DNA level and splice out at the protein level. Once thought of as only a parasitic type of a mobile genetic element, recent work suggests a mutualistic relationship has formed in some cases within bacterial and archaeal hosts. After translation, a precursor protein is formed with the intein between two exteins. The intein is catalytic and can excise itself out through protein splicing. Intein insertion is biased towards the active site of the protein and is thought to cause inactivation of the host protein prior to splicing. Intein splicing is responsive to a …
Studies Of Entry, Reverse Transcription, And Regulation Of Splicing In Retroviruses, Timothy A. Sullivan
Studies Of Entry, Reverse Transcription, And Regulation Of Splicing In Retroviruses, Timothy A. Sullivan
Theses and Dissertations (ETD)
The study of retroviruses and their lifecycle has contributed immensely to our knowledge of the world of biology and medicine. The central dogma of the basic flow of genetic information was shattered when the discovery that retroviruses copy their RNA genome into DNA was made. The same enzyme that performs this step, reverse transcriptase (RT), also revolutionized molecular biology when it was used as a tool to generate full length cDNA clones of expressed genes. The impact of retroviruses on the medical field has been extremely exciting as the ideas of using retroviral vectors to deliver genes providing long term …