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Full-Text Articles in Organisms

Analyzing Wisp Dna Sequences And Building Primers For Dna Separation, Paige Spicer, Payton Crum Apr 2024

Analyzing Wisp Dna Sequences And Building Primers For Dna Separation, Paige Spicer, Payton Crum

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A bacteriophage is a virus that infects bacteria. Bacteriophage DNA was isolated from a lysate sample, but two separate, unrelated genome sequences were returned. Plaque assays and plaque streaking were used to in an effort to separate the phages. After plaque isolation was unsuccessful, NCBI BLAST and IDT software were used to build unique primers for each of the two unrelated genomes. After receiving the primers, PCR and gel electrophoresis were run on plaques and lysate from each phage to classify each phage in the correct cluster and sub cluster as identified by the original DNA sequencing.


Using Oncodb's Oncovirus Analysis For Hbv-Related Hcc, Anna Claire Newman Apr 2024

Using Oncodb's Oncovirus Analysis For Hbv-Related Hcc, Anna Claire Newman

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Since one of the known causes of cancer is virus infection causing changes in gene regulation, being able to compare the expression and methylation of regular tumors and virus-related tumors could provide much needed information into virus induced cancer such as HBV-related HCC. OncoDB's oncovirus analysis capabilities can provide information and research opportunities that past research has not had.


Progress Towards Phage Stability For Possible Oral Phage Therapy, Erin Russo Apr 2024

Progress Towards Phage Stability For Possible Oral Phage Therapy, Erin Russo

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Oral cavities or caries is a localized deterioration of the tooth caused by an accumulation of harmful bacteria. This research intends to integrate bacteriophages onto unwaxed dental floss as a possible future preventative treatment for oral cavities. Bacteriophages are viruses that degrade the composition of bacterial populations by invading and reproducing inside bacterial hosts. The experimental framework involves soaking unwaxed floss in the bacteriophages named Phrick and Stonehill. The effectiveness of infection is measured by applying the phage-infused floss on an agar plate with Gordonia terrae CAG3, then analyzing the diameter of the plaque around the floss. This research will …


Growth And Production Of Spirulina Plantesis Biomass At The Same Light Intensity And Temperature, Makayla Miller Apr 2023

Growth And Production Of Spirulina Plantesis Biomass At The Same Light Intensity And Temperature, Makayla Miller

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No abstract provided.


The Effects Of Light Intensity On Cell Population And Oxygen Production Of Arthrospira Platensis, Sophia Ward, Cole Turner Apr 2023

The Effects Of Light Intensity On Cell Population And Oxygen Production Of Arthrospira Platensis, Sophia Ward, Cole Turner

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As space exploration becomes more advanced, scientists look to find new ways to create nourishment and oxygen to maintain long-term space travel. Arthrospira platensis, commonly known as Spirulina, could be a possible solution to these problems. This cyanobacterium converts carbon dioxide into pure oxygen and the cell structure (coiled or straight) appears to trigger different oxygen and cultivation responses. Mixed (coiled and straight, Fig. 4) and coiled (Fig. 5) Spirulina cultures were transferred into 2.5 L containers and placed in white light boxes with varying intensities of light: 15 µmol/㎡/s, 7 µmol/㎡/s, and 3 µmol/㎡/s. Each experiment lasted 72 hours …


The Effects Of Light Intensity And Cell Structure On The Cultivation Of Arthrospira Platensis, Taylor Barnhart Apr 2023

The Effects Of Light Intensity And Cell Structure On The Cultivation Of Arthrospira Platensis, Taylor Barnhart

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As scientists explore further into space, more cost-effective resources are needed for long-term space travel. An interesting solution is Arthrospira platensis, a filamentous cyanobacteria high in proteins and nutrients, and known for its helical structure. In unfavorable conditions, coiled spirulina cells become straight. Spirulina converts carbon dioxide gas into pure oxygen and the different cell structures stimulate different responses in oxygen production and cultivation. In these experiments, 2.3 L containers of pure coiled spirulina and mixed (coiled and straight) spirulina were placed into 3 incubators with different light intensities: 51µmol/m2/s, 25µmol/m2/s, 12µmol/m2/s. Each experiment length was 72 hours and the …


Bacteriophages: Paving The Road For The Future Of Medicine, Luke Brinkerhoff Apr 2022

Bacteriophages: Paving The Road For The Future Of Medicine, Luke Brinkerhoff

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Antibiotics will not be used forever by society due to antibiotic resistance. By the year 2050, it is predicted to have caused millions of casualties throughout the world. Thankfully, bacteriophages and phage therapy could be a potential solution to this problem.


New Paenibacillus Bacteria Genome Assembly, Makenna Kager Apr 2022

New Paenibacillus Bacteria Genome Assembly, Makenna Kager

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Due to DLA16’s ability to produce antibiotics it was selected for whole genome sequencing.