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Full-Text Articles in Radiology
Marcks Regulates Growth And Radiation Sensitivity And Is A Novel Prognostic Factor For Glioma, James S. Jardoe, Joshua C. Anderson, Christine W. Duarte, Tapan Mehta, Somaira Nowsheen, Patrick H. Hicks, Alexander C. Whitley, Timothy Rohrbach, Raymond O. Mccubrey, Sherard Chiu, Tamara M. Burleson, James A. Bonner, G. Yancey Gillespie, Eddy S. Yang, Christopher D. Willey
Marcks Regulates Growth And Radiation Sensitivity And Is A Novel Prognostic Factor For Glioma, James S. Jardoe, Joshua C. Anderson, Christine W. Duarte, Tapan Mehta, Somaira Nowsheen, Patrick H. Hicks, Alexander C. Whitley, Timothy Rohrbach, Raymond O. Mccubrey, Sherard Chiu, Tamara M. Burleson, James A. Bonner, G. Yancey Gillespie, Eddy S. Yang, Christopher D. Willey
Richard Bland Faculty Works
Purpose
This study assessed whether Myristoylated Alanine Rich C-Kinase Substrate (MARCKS) can regulate glioblastoma (GBM) growth, radiation sensitivity and clinical outcome.
Experimental Design
MARCKS protein levels were analyzed in five GBM explant cell lines and eight patient-derived xenograft tumors by immunoblot, and these levels were correlated to proliferation rates and intracranial growth rates, respectively. Manipulation of MARCKS protein levels was assessed by lentiviral-mediated shRNA knockdown in the U251 cell line and MARCKS over-expression in the U87 cell line. The effect of manipulation of MARCKS on proliferation, radiation sensitivity and senescence was assessed. MARCKS gene expression was correlated with survival outcomes …