Pathology Commons^™

Synovial Histopathology Across A Spectrum Of Hip Disorders: From Pre-Arthritic Femoroacetabular Impingement To Advanced Osteoarthritis, Ronan James Anderson

Electronic Thesis and Dissertation Repository

Objective: The objective of this thesis was to provide insight into early changes in osteoarthritis pathogenesis. Histopathology of synovium was investigated in femoroacetabular impingement (FAI), FAI with early hip osteoarthritis, and advanced hip osteoarthritis.

Methods: Synovium biopsies were collected from ten FAI, fourteen FAI with early osteoarthritis, and twelve advanced osteoarthritis patients. Histopathological grading allowed assessment of osteoarthritis-associated features. Microvessel density and maturity were determined through immunofluorescent labelling of CD31 and a-smooth muscle actin. Immunohistochemical staining was applied to calculate CD105⁺ microvessel density.

Results: In all groups, vascularization was prominent, with a mean [95% confidence interval] of 1.66 [1.41, …

Assessing Inflammation In The Pathology Of Knee Osteoarthritis, Zachary J. Koudys

Electronic Thesis and Dissertation Repository

Osteoarthritis (OA) is a joint disease that causes pain, stiffness, and loss of function. Inflammation of the synovium plays a role in the pathology of OA. Macrophages are the dominant immune cells in synovial tissue. Activated macrophages over-express the translocator protein (TSPO). [¹⁸F]FEPPA is a 2^nd generation positron emission tomography (PET) tracer that can target TSPO with high specificity. Hybrid [¹⁸F]FEPPA PET/MRI may enable accurate quantification of macrophage activity in vivo. In this work, [¹⁸F]FEPPA tracer uptake in knee synovial tissue was measured ex vivo using autoradiography and was validated to correlate to …