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Articles 1 - 3 of 3
Full-Text Articles in Ophthalmology
Thalidomide Prevents Donor Corneal Graft Neovascularization In An Alkali Burn Model Of Corneal Angiogenesis, A. Abbas, Aga Hassan Feroze, G. F. Hyman, B. Khan
Thalidomide Prevents Donor Corneal Graft Neovascularization In An Alkali Burn Model Of Corneal Angiogenesis, A. Abbas, Aga Hassan Feroze, G. F. Hyman, B. Khan
Section of Ophthalmology
Objective: Thalidomide is a potent inhibitor of angiogenesis. We evaluated the effects of Thalidomide on corneal angiogenesis and on tissue survival of grafts in rabbit eyes with pre-existing neovascularization secondary to alkali burn.
Methods: Sixteen rabbits received alkali burns to one cornea. One month post-injury, assessments of corneal neovascularization were performed followed by corneal transplantation. Four rabbits received oral Thalidomide and ten got placebo (powdered sugar) for thirty days. Total corneal neovascularization (NV), clock hours (CH) involved in (NV), longest (NV) pedicle length (NVP) and the duration of time required for NV to develop were assessed.
Results: Thalidomide significantly decreased …
Macular Hemorrhage Secondary To Increased Intrathoracic Pressure And Difficult Intubation, A Abbas, G Hyman
Macular Hemorrhage Secondary To Increased Intrathoracic Pressure And Difficult Intubation, A Abbas, G Hyman
Section of Ophthalmology
No abstract provided.
Polymerase Chain Reaction: An Emerging Tool For Research In Pharmacology, Rishi Sharma, Manjeet Singh, Ajay Sharma
Polymerase Chain Reaction: An Emerging Tool For Research In Pharmacology, Rishi Sharma, Manjeet Singh, Ajay Sharma
Pharmacy Faculty Articles and Research
Polymerase chain reaction (PCR) is a technique that can amplify a specific DNA segment in vitro using two site specific primers that hybridise to opposite DNA strands. The method produces large amounts of specific DNA from a complex DNA template in a single enzymatic reaction within a matter of hours. Reaction mixture contains a double helix DNA, two target specific primers, dNTPs, a DNA polymerase and a Mg2+ containing buffer. PCR is carried out in three distinct steps i.e. denaturation step, annealing step and extension step, which are repeated many times. The efficiency of PCR is measured in terms of …