Medical Specialties Commons^™

A Narrative Review On Yoga: A Potential Intervention For Augmenting Immunomodulation And Mental Health In Covid-19, Indranill Basu-Ray, Kashinath Metri, Dibbendhu Khanra, Rishab Revankar, Kavitha M Chinnaiyan, Nagaratna Raghuram, Mahesh Chandra Mishra, Bhushan Patwardhan, Manjunath Sharma, Ishwar V Basavaraddi, Akshay Anand, Shrinath Reddy, K K Deepak, Marian Levy, Sue Theus, Glenn N Levine, Holger Cramer, Gregory L Fricchione, Nagendra R Hongasandra

Faculty, Staff and Students Publications

BACKGROUND: The ongoing novel coronavirus disease 2019 (COVID-19) pandemic has a significant mortality rate of 3-5%. The principal causes of multiorgan failure and death are cytokine release syndrome and immune dysfunction. Stress, anxiety, and depression has been aggravated by the pandemic and its resultant restrictions in day-to-day life which may contribute to immune dysregulation. Thus, immunity strengthening and the prevention of cytokine release syndrome are important for preventing and minimizing mortality in COVID-19 patients. However, despite a few specific remedies that now exist for the SARS-CoV-2virus, the principal modes of prevention include vaccination, masking, and holistic healing methods, such as …

Scintillation Light Detection In The 6-M Drift-Length Protodune Dual Phase Liquid Argon Tpc, A Abed Abud, B Abi, R Acciarri, M A Acero, M R Adames, G Adamov, M Adamowski, D Adams, M Adinolfi, A Aduszkiewicz, J Aguilar, Z Ahmad, J Ahmed, B Aimard, B Ali-Mohammadzadeh, T Alion, K Allison, S Alonso Monsalve, M Alrashed, C Alt, A Alton, R Alvarez, P Amedo, J Anderson, C Andreopoulos, M Andreotti, M Andrews, F Andrianala, S Andringa, N Anfimov, A Ankowski, M Antoniassi, M Antonova, A Antoshkin, S Antusch, A Aranda-Fernandez, L Arellano, L O Arnold, M A Arroyave, J Asaadi, L Asquith, A Aurisano, V Aushev, D Autiero, V Ayala Lara, M Ayala-Torres, F Azfar, A Back, H Back, J J Back, C Backhouse, I Bagaturia, L Bagby, N Balashov, S Balasubramanian, P Baldi, B Baller, B Bambah, F Barao, G Barenboim, P Barham Alzas, G Barker, W Barkhouse, C Barnes, G Barr, J Barranco Monarca, A Barros, N Barros, J L Barrow, A Basharina-Freshville, A Bashyal, V Basque, C Batchelor, E Batista Das Chagas, J B R Battat, F Battisti, F Bay, M C Q Bazetto, J L L Bazo Alba, J F Beacom, E Bechetoille, B Behera, C Beigbeder, L Bellantoni, G Bellettini, V Bellini, O Beltramello, N Benekos, C Benitez Montiel, F Bento Neves, J Berger, S Berkman, P Bernardini, R M Berner, A Bersani, S Bertolucci, M Betancourt, A Betancur Rodríguez, A Bevan, Y Bezawada, T J C Bezerra, A Bhardwaj, V Bhatnagar, M Bhattacharjee, D Bhattarai, S Bhuller, B Bhuyan, S Biagi, J Bian, M Biassoni, K Biery, B Bilki, M Bishai, A Bitadze, A Blake, F Blaszczyk, G C Blazey, E Blucher, J Boissevain, S Bolognesi, T Bolton, L Bomben, M Bonesini, M Bongrand, C Bonilla-Diaz, F Bonini, A Booth, F Boran, S Bordoni, A Borkum, N Bostan, P Bour, C Bourgeois, D Boyden, J Bracinik, D Braga, D Brailsford, A Branca, A Brandt, J Bremer, D Breton, C Brew, S J Brice, C Brizzolari, C Bromberg, J Brooke, A Bross, G Brunetti, M Brunetti, N Buchanan, H Budd, I Butorov, I Cagnoli, T Cai, D Caiulo, R Calabrese, P Calafiura, J Calcutt, M Calin, S Calvez, E Calvo, A Caminata, M Campanelli, D Caratelli, D Carber, J C Carceller, G Carini, B Carlus, M F Carneiro, P Carniti, I Caro Terrazas, H Carranza, T Carroll, J F Castaño Forero, A Castillo, C Castromonte, E Catano-Mur, C Cattadori, F Cavalier, G Cavallaro, F Cavanna, S Centro, G Cerati, A Cervelli, A Cervera Villanueva, M Chalifour, A Chappell, E Chardonnet, N Charitonidis, A Chatterjee, S Chattopadhyay, M S S Chavarry Neyra, H Chen, M Chen, Y Chen, Z Chen, Z Chen-Wishart, Y Cheon, D Cherdack, C Chi, S Childress, R Chirco, A Chiriacescu, G Chisnall, K Cho, S Choate, D Chokheli, P S Chong, A Christensen, D Christian, G Christodoulou, A Chukanov, M Chung, E Church, V Cicero, P Clarke, G Cline, T E Coan, A G Cocco, J A B Coelho, N Colton, E Conley, R Conley, J Conrad, M Convery, S Copello, P Cova, L Cremaldi, L Cremonesi, J I Crespo-Anadón, M Crisler, E Cristaldo, J Crnkovic, R Cross, A Cudd, C Cuesta, Y Cui, D Cussans, O Dalager, H Da Motta, L Da Silva Peres, C David, Q David, G S Davies, S Davini, J Dawson, K De, S De, P Debbins, I De Bonis, M P Decowski, A De Gouvêa, P C De Holanda, I L De Icaza Astiz, A Deisting, P De Jong, A Delbart, D Delepine, M Delgado, A Dell'acqua, N Delmonte, P De Lurgio, J R T De Mello Neto, D M Demuth, S Dennis, C Densham, G W Deptuch, A De Roeck, V De Romeri, G De Souza, R Devi, R Dharmapalan, M Dias, F Diaz, J S Díaz, S Di Domizio, L Di Giulio, P Ding, L Di Noto, G Dirkx, C Distefano, R Diurba, M Diwan, Z Djurcic, D Doering, S Dolan, F Dolek, M Dolinski, L Domine, Y Donon, D Douglas, D Douillet, A Dragone, G Drake, F Drielsma, L Duarte, D Duchesneau, K Duffy, P Dunne, B Dutta, H Duyang, O Dvornikov, D Dwyer, A Dyshkant, M Eads, A Earle, D Edmunds, J Eisch, L Emberger, S Emery, P Englezos, A Ereditato, T Erjavec, C Escobar, G Eurin, J J Evans, E Ewart, A C Ezeribe, K Fahey, A Falcone, M Fani', C Farnese, Y Farzan, D Fedoseev, J Felix, Y Feng, E Fernandez-Martinez, P Fernandez Menendez, M Fernandez Morales, F Ferraro, L Fields, P Filip, F Filthaut, M Fiorini, V Fischer, R S Fitzpatrick, W Flanagan, B Fleming, R Flight, S Fogarty, W Foreman, J Fowler, W Fox, J Franc, K Francis, D Franco, J Freeman, J Freestone, J Fried, A Friedland, F Fuentes Robayo, S Fuess, I K Furic, K Furman, A P Furmanski, A Gabrielli, A Gago, H Gallagher, A Gallas, A Gallego-Ros, N Gallice, V Galymov, E Gamberini, T Gamble, F Ganacim, R Gandhi, R Gandrajula, F Gao, S Gao, D Garcia-Gamez, M Á García-Peris, S Gardiner, D Gastler, J Gauvreau, G Ge, N Geffroy, B Gelli, A Gendotti, S Gent, Z Ghorbani-Moghaddam, P Giammaria, T Giammaria, N Giangiacomi, D Gibin, I Gil-Botella, S Gilligan, C Girerd, A K Giri, D Gnani, O Gogota, M Gold, S Gollapinni, K Gollwitzer, R A Gomes, L V Gomez Bermeo, L S Gomez Fajardo, F Gonnella, D Gonzalez-Diaz, M Gonzalez-Lopez, M C Goodman, O Goodwin, S Goswami, C Gotti, E Goudzovski, C Grace, R Gran, E Granados, P Granger, A Grant, C Grant, D Gratieri, P Green, L Greenler, J Greer, J Grenard, W C Griffith, M Groh, J Grudzinski, K Grzelak, W Gu, E Guardincerri, V Guarino, M Guarise, R Guenette, E Guerard, M Guerzoni, D Guffanti, A Guglielmi, B Guo, A Gupta, V Gupta, K K Guthikonda, R Gutierrez, P Guzowski, M M Guzzo, S Gwon, C Ha, K Haaf, A Habig, H Hadavand, R Haenni, A Hahn, J Haiston, P Hamacher-Baumann, T Hamernik, P Hamilton, J Han, D A Harris, J Hartnell, T Hartnett, J Harton, T Hasegawa, C Hasnip, R Hatcher, K W Hatfield, A Hatzikoutelis, C Hayes, K Hayrapetyan, J Hays, E Hazen, M He, A Heavey, K M Heeger, J Heise, S Henry, M A Hernandez Morquecho, K Herner, J Hewes, C Hilgenberg, T Hill, S J Hillier, A Himmel, E Hinkle, L R Hirsch, J Ho, J Hoff, A Holin, E Hoppe, G A Horton-Smith, M Hostert, A Hourlier, B Howard, R Howell, J Hoyos, I Hristova, M S Hronek, J Huang, Z Hulcher, G Iles, N Ilic, A M Iliescu, R Illingworth, G Ingratta, A Ioannisian, B Irwin, L Isenhower, R Itay, C M Jackson, V Jain, E James, W Jang, B Jargowsky, F Jediny, D Jena, Y S Jeong, C Jesús-Valls, X Ji, L Jiang, S Jiménez, A Jipa, R Johnson, W Johnson, N Johnston, B Jones, S Jones, M Judah, C K Jung, T Junk, Y Jwa, M Kabirnezhad, A Kaboth, I Kadenko, I Kakorin, A Kalitkina, D Kalra, F Kamiya, N Kaneshige, D M Kaplan, G Karagiorgi, G Karaman, A Karcher, M Karolak, Y Karyotakis, S Kasai, S P Kasetti, L Kashur, N Kazaryan, E Kearns, P Keener, K J Kelly, E Kemp, O Kemularia, W Ketchum, S H Kettell, M Khabibullin, A Khotjantsev, A Khvedelidze, D Kim, B King, B Kirby, M Kirby, J Klein, A Klustova, T Kobilarcik, K Koehler, L W Koerner, D H Koh, S Kohn, P P Koller, L Kolupaeva, D Korablev, M Kordosky, T Kosc, U Kose, V A Kostelecký, K Kothekar, R Kralik, L Kreczko, F Krennrich, I Kreslo, W Kropp, T Kroupova, S Kubota, Y Kudenko, V A Kudryavtsev, S Kulagin, J Kumar, P Kumar, P Kunze, N Kurita, C Kuruppu, V Kus, T Kutter, J Kvasnicka, D Kwak, A Lambert, B Land, C E Lane, K Lang, T Langford, M Langstaff, J Larkin, P Lasorak, D Last, A Laundrie, G Laurenti, A Lawrence, I Lazanu, R Lazur, M Lazzaroni, T Le, S Leardini, J Learned, P Lebrun, T Lecompte, C Lee, S Y Lee, G Lehmann Miotto, R Lehnert, M A Leigui De Oliveira, M Leitner, L M Lepin, S W Li, Y Li, H Liao, C S Lin, Q Lin, S Lin, R A Lineros, J Ling, A Lister, B R Littlejohn, J Liu, Y Liu, S Lockwitz, T Loew, M Lokajicek, I Lomidze, K Long, T Lord, J M Losecco, W C Louis, X-G Lu, K B Luk, B Lunday, X Luo, E Luppi, T Lux, V P Luzio, J Maalmi, D Macfarlane, A A Machado, P Machado, C T Macias, J R Macier, A Maddalena, A Madera, P Madigan, S Magill, K Mahn, A Maio, A Major, J A Maloney, G Mandrioli, R C Mandujano, J Maneira, L Manenti, S Manly, A Mann, K Manolopoulos, M Manrique Plata, V N Manyam, L Manzanillas, M Marchan, A Marchionni, W Marciano, D Marfatia, C Mariani, J Maricic, R Marie, F Marinho, A D Marino, D Marsden, M Marshak, C Marshall, J Marshall, J Marteau, J Martín-Albo, N Martinez, D A Martinez Caicedo, P Martínez Miravé, S Martynenko, V Mascagna, K Mason, A Mastbaum, F Matichard, S Matsuno, J Matthews, C Mauger, N Mauri, K Mavrokoridis, I Mawby, R Mazza, A Mazzacane, E Mazzucato, T Mcaskill, E Mccluskey, N Mcconkey, K S Mcfarland, C Mcgrew, A Mcnab, A Mefodiev, P Mehta, P Melas, O Mena, H Mendez, P Mendez, D P Méndez, A Menegolli, G Meng, M D Messier, W Metcalf, T Mettler, M Mewes, H Meyer, T Miao, G Michna, T Miedema, V Mikola, R Milincic, G Miller, W Miller, J Mills, O Mineev, A Minotti, O G Miranda, S Miryala, C S Mishra, S R Mishra, A Mislivec, M Mitchell, D Mladenov, I Mocioiu, K Moffat, N Moggi, R Mohanta, T A Mohayai, N Mokhov, J Molina, L Molina Bueno, E Montagna, A Montanari, C Montanari, D Montanari, L M Montañno Zetina, S H Moon, M Mooney, A F Moor, D Moreno, D Moretti, C Morris, C Mossey, M Mote, E Motuk, C A Moura, J Mousseau, G Mouster, W Mu, L Mualem, J Mueller, M Muether, S Mufson, F Muheim, A Muir, M Mulhearn, D Munford, H Muramatsu, S Murphy, J Musser, J Nachtman, S Nagu, M Nalbandyan, R Nandakumar, D Naples, S Narita, A Nath, A Navrer-Agasson, N Nayak, M Nebot-Guinot, K Negishi, J K Nelson, J Nesbit, M Nessi, D Newbold, M Newcomer, H Newton, R Nichol, F Nicolas-Arnaldos, A Nikolica, E Niner, K Nishimura, A Norman, A Norrick, R Northrop, P Novella, J A Nowak, M Oberling, J Ochoa-Ricoux, A Olivier, A Olshevskiy, Y Onel, Y Onishchuk, J Ott, L Pagani, G Palacio, O Palamara, S Palestini, J M Paley, M Pallavicini, C Palomares, W Panduro Vazquez, E Pantic, V Paolone, V Papadimitriou, R Papaleo, A Papanestis, S Paramesvaran, S Parke, E Parozzi, Z Parsa, M Parvu, S Pascoli, L Pasqualini, J Pasternak, J Pater, C Patrick, L Patrizii, R B Patterson, S J Patton, T Patzak, A Paudel, B Paulos, L Paulucci, Z Pavlovic, G Pawloski, D Payne, V Pec, S J M Peeters, A Pena Perez, E Pennacchio, A Penzo, O L G Peres, J Perry, D Pershey, G Pessina, G Petrillo, C Petta, R Petti, V Pia, F Piastra, L Pickering, F Pietropaolo, V L Pimentel, G Pinaroli, K Plows, R Plunkett, R Poling, F Pompa, X Pons, N Poonthottathil, F Poppi, S Pordes, J Porter, M Potekhin, R Potenza, B V K S Potukuchi, J Pozimski, M Pozzato, S Prakash, T Prakash, M Prest, S Prince, F Psihas, D Pugnere, X Qian, J L Raaf, V Radeka, J Rademacker, B Radics, A Rafique, E Raguzin, M Rai, M Rajaoalisoa, I Rakhno, A Rakotonandrasana, L Rakotondravohitra, R Rameika, M A Ramirez Delgado, B Ramson, A Rappoldi, G Raselli, P Ratoff, S Raut, R F Razakamiandra, E M Rea, J S Real, B Rebel, R Rechenmacher, M Reggiani-Guzzo, J Reichenbacher, S D Reitzner, H Rejeb Sfar, A Renshaw, S Rescia, F Resnati, M Ribas, S Riboldi, C Riccio, G Riccobene, L C J Rice, J S Ricol, A Rigamonti, Y Rigaut, E V Rincón, H Ritchie-Yates, D Rivera, A Robert, L Rochester, M Roda, P Rodrigues, M J Rodriguez Alonso, E Rodriguez Bonilla, J Rodriguez Rondon, S Rosauro-Alcaraz, M Rosenberg, P Rosier, B Roskovec, M Rossella, M Rossi, J Rout, P Roy, A Rubbia, C Rubbia, B Russell, D Ruterbories, A Rybnikov, A Saa-Hernandez, R Saakyan, S Sacerdoti, T Safford, N Sahu, K Sakashita, P Sala, N Samios, O Samoylov, M C Sanchez, V Sandberg, D A Sanders, D Sankey, S Santana, M Santos-Maldonado, N Saoulidou, P Sapienza, C Sarasty, I Sarcevic, G Savage, V Savinov, A Scaramelli, A Scarff, A Scarpelli, T Schefke, H Schellman, S Schifano, P Schlabach, D Schmitz, A W Schneider, K Scholberg, A Schukraft, E Segreto, A Selyunin, C R Senise, J Sensenig, A Sergi, D Sgalaberna, M H Shaevitz, S Shafaq, F Shaker, M Shamma, R Sharankova, H R Sharma, R Sharma, R K Sharma, T Shaw, K Shchablo, C Shepherd-Themistocleous, A Sheshukov, S Shin, I Shoemaker, D Shooltz, R Shrock, H Siegel, L Simard, J Sinclair, G Sinev, J Singh, L Singh, P Singh, V Singh, R Sipos, F W Sippach, G Sirri, A Sitraka, K Siyeon, K Skarpaas, A Smith, E Smith, P Smith, J Smolik, M Smy, E Snider, P Snopok, D Snowden-Ifft, M Soares Nunes, H Sobel, M Soderberg, S Sokolov, C J Solano Salinas, S Söldner-Rembold, S R Soleti, N Solomey, V Solovov, W E Sondheim, M Sorel, A Sotnikov, J Soto-Oton, F A Soto Ugaldi, A Sousa, K Soustruznik, F Spagliardi, M Spanu, J Spitz, N J C Spooner, K Spurgeon, M Stancari, L Stanco, C Stanford, R Stein, H M Steiner, A F Steklain Lisbôa, J Stewart, B Stillwell, J Stock, F Stocker, T Stokes, M Strait, T Strauss, L Strigari, A Stuart, J G Suarez, J M Suárez Sunción, H Sullivan, D Summers, A Surdo, V Susic, L Suter, C M Sutera, R Svoboda, B Szczerbinska, A M Szelc, H Tanaka, S Tang, A Tapia, B Tapia Oregui, A Tapper, S Tariq, E Tarpara, N Tata, E Tatar, R Tayloe, A M Teklu, P Tennessen, M Tenti, K Terao, C A Ternes, F Terranova, G Testera, T Thakore, A Thea, J L Thompson, C Thorn, S C Timm, V Tishchenko, L Tomassetti, A Tonazzo, D Torbunov, M Torti, M Tortola, F Tortorici, N Tosi, D Totani, M Toups, C Touramanis, R Travaglini, J Trevor, S Trilov, W H Trzaska, Y Tsai, Y-T Tsai, Z Tsamalaidze, K V Tsang, N Tsverava, S Tufanli, C Tull, E Tyley, M Tzanov, L Uboldi, M A Uchida, J Urheim, T Usher, S Uzunyan, M R Vagins, P Vahle, S Valder, G D A Valdiviesso, E Valencia, R Valentim, Z Vallari, E Vallazza, J W F Valle, S Vallecorsa, R Van Berg, R G Van De Water, D Vanegas Forero, D Vannerom, F Varanini, D Vargas Oliva, G Varner, J Vasel, S Vasina, G Vasseur, N Vaughan, K Vaziri, S Ventura, A Verdugo, S Vergani, M A Vermeulen, M Verzocchi, M Vicenzi, H Vieira De Souza, C Vignoli, C Vilela, B Viren, T Vrba, T Wachala, A V Waldron, M Wallbank, C Wallis, H Wang, J Wang, L Wang, M H L S Wang, X Wang, Y Wang, K Warburton, D Warner, M O Wascko, D Waters, A Watson, K Wawrowska, P Weatherly, A Weber, M Weber, H Wei, A Weinstein, D Wenman, M Wetstein, A White, L H Whitehead, D Whittington, M J Wilking, A Wilkinson, C Wilkinson, Z Williams, F Wilson, R J Wilson, W Wisniewski, J Wolcott, T Wongjirad, A Wood, K Wood, E Worcester, M Worcester, K Wresilo, C Wret, W Wu, Y Xiao, F Xie, B Yaeggy, E Yandel, G Yang, K Yang, T Yang, A Yankelevich, N Yershov, K Yonehara, Y S Yoon, T Young, B Yu, H Yu, J Yu, Y Yu, W Yuan, R Zaki, J Zalesak, L Zambelli, B Zamorano, A Zani, L Zazueta, G P Zeller, J Zennamo, K Zeug, C Zhang, S Zhang, Y Zhang, M Zhao, E Zhivun, G Zhu, E D Zimmerman, S Zucchelli, J Zuklin, V Zutshi, R Zwaska, Dune Collaboration

Faculty, Staff and Students Publications

DUNE is a dual-site experiment for long-baseline neutrino oscillation studies, neutrino astrophysics and nucleon decay searches. ProtoDUNE Dual Phase (DP) is a 6 × 6 × 6 m3 liquid argon time-projection-chamber (LArTPC) that recorded cosmic-muon data at the CERN Neutrino Platform in 2019–2020 as a prototype of the DUNE Far Detector. Charged particles propagating through the LArTPC produce ionization and scintillation light. The scintillation light signal in these detectors can provide the trigger for non-beam events. In addition, it adds precise timing capabilities and improves the calorimetry measurements. In ProtoDUNE-DP, scintillation and electroluminescence light produced by cosmic muons in the …

Triplet Therapy, Transplantation, And Maintenance Until Progression In Myeloma, Paul G Richardson, Susanna J Jacobus, Edie A Weller, Hani Hassoun, Sagar Lonial, Noopur S Raje, Eva Medvedova, Philip L Mccarthy, Edward N Libby, Peter M Voorhees, Robert Z Orlowski, Larry D Anderson, Jeffrey A Zonder, Carter P Milner, Cristina Gasparetto, Mounzer E Agha, Abdullah M Khan, David D Hurd, Krisstina Gowin, Rammurti T Kamble, Sundar Jagannath, Nitya Nathwani, Melissa Alsina, R Frank Cornell, Hamza Hashmi, Erica L Campagnaro, Astrid C Andreescu, Teresa Gentile, Michaela Liedtke, Kelly N Godby, Adam D Cohen, Thomas H Openshaw, Marcelo C Pasquini, Sergio A Giralt, Jonathan L Kaufman, Andrew J Yee, Emma Scott, Pallawi Torka, Amy Foley, Mariateresa Fulciniti, Kyle Hebert, Mehmet K Samur, Kelly Masone, Michelle E Maglio, Andrea A Zeytoonjian, Omar Nadeem, Robert L Schlossman, Jacob P Laubach, Claudia Paba-Prada, Irene M Ghobrial, Aurore Perrot, Philippe Moreau, Hervé Avet-Loiseau, Michel Attal, Kenneth C Anderson, Nikhil C Munshi, Determination Investigators

Faculty, Staff and Students Publications

BACKGROUND: In patients with newly diagnosed multiple myeloma, the effect of adding autologous stem-cell transplantation (ASCT) to triplet therapy (lenalidomide, bortezomib, and dexamethasone [RVD]), followed by lenalidomide maintenance therapy until disease progression, is unknown.

METHODS: In this phase 3 trial, adults (18 to 65 years of age) with symptomatic myeloma received one cycle of RVD. We randomly assigned these patients, in a 1:1 ratio, to receive two additional RVD cycles plus stem-cell mobilization, followed by either five additional RVD cycles (the RVD-alone group) or high-dose melphalan plus ASCT followed by two additional RVD cycles (the transplantation group). Both groups received …

The Testis-Specific E3 Ubiquitin Ligase Rnf133 Is Required For Fecundity In Mice, Kaori Nozawa, Yoshitaka Fujihara, Darius J Devlin, Ricardo E Deras, Katarzyna Kent, Irina V Larina, Kohei Umezu, Zhifeng Yu, Courtney M Sutton, Qiuji Ye, Laura K Dean, Chihiro Emori, Masahito Ikawa, Thomas X Garcia, Martin M Matzuk

Faculty, Staff and Students Publications

BACKGROUND: Ubiquitination is a post-translational modification required for a number of physiological functions regulating protein homeostasis, such as protein degradation. The endoplasmic reticulum (ER) quality control system recognizes and degrades proteins no longer needed in the ER through the ubiquitin-proteasome pathway. E2 and E3 enzymes containing a transmembrane domain have been shown to function in ER quality control. The ER transmembrane protein UBE2J1 is a E2 ubiquitin-conjugating enzyme reported to be essential for spermiogenesis at the elongating spermatid stage. Spermatids from Ube2j1 KO male mice are believed to have defects in the dislocation step of ER quality control. However, associated …

Internal Standard Triggered-Parallel Reaction Monitoring Mass Spectrometry Enables Multiplexed Quantification Of Candidate Biomarkers In Plasma, Jacob J Kennedy, Jeffrey R Whiteaker, Richard G Ivey, Aura Burian, Shrabanti Chowdhury, Chia-Feng Tsai, Tao Liu, Chenwei Lin, Oscar D Murillo, Rachel A Lundeen, Lisa A Jones, Philip R Gafken, Gary Longton, Karin D Rodland, Steven J Skates, John Landua, Pei Wang, Michael T Lewis, Amanda G Paulovich

Faculty, Staff and Students Publications

Despite advances in proteomic technologies, clinical translation of plasma biomarkers remains low, partly due to a major bottleneck between the discovery of candidate biomarkers and costly clinical validation studies. Due to a dearth of multiplexable assays, generally only a few candidate biomarkers are tested, and the validation success rate is accordingly low. Previously, mass spectrometry-based approaches have been used to fill this gap but feature poor quantitative performance and were generally limited to hundreds of proteins. Here, we demonstrate the capability of an internal standard triggered-parallel reaction monitoring (IS-PRM) assay to greatly expand the numbers of candidates that can be …

Selected Transesophageal Echocardiographic Parameters Of Left Ventricular Diastolic Function Predict Length Of Stay Following Coronary Artery Bypass Graft-A Prospective Observational Study, Samhati Mondal, Nauder Faraday, Wei Dong Gao, Sarabdeep Singh, Sachidanand Hebbar, Kimberly N Hollander, Thomas S Metkus, Lee A Goeddel, Maria Bauer, Brian Bush, Brian Cho, Stephanie Cha, Stephanie O Ibekwe, Domagoj Mladinov, Noah S Rolleri, Laeben Lester, Jochen Steppan, Rosanne Sheinberg, Nadia B Hensley, Anubhav Kapoor, Jeffrey M Dodd-O

Faculty, Staff and Students Publications

(1) Importance: Abnormal left ventricular (LV) diastolic function, with or without a diagnosis of heart failure, is a common finding that can be easily diagnosed by intra-operative transesophageal echocardiography (TEE). The association of diastolic function with duration of hospital stay after coronary artery bypass (CAB) is unknown. (2) Objective: To determine if selected TEE parameters of diastolic dysfunction are associated with length of hospital stay after coronary artery bypass surgery (CAB). (3) Design: Prospective observational study. (4) Setting: A single tertiary academic medical center. (5) Participants: Patients with normal systolic function undergoing isolated CAB from September 2017 through June 2018. …

Maternal Adipocyte Connexin43 Gap Junctions Affect Breastmilk Lactose Levels And Neonate Growth In Mice, Mingyang Huang, Anying Song, Xi Chen, Sarah Ishtiaq, Chunmei Wang, Darryl L Hadsell, Qiong A Wang, Yi Zhu

Faculty, Staff and Students Publications

Simple Summary

Breastfeeding offers many health benefits for both mothers and infants. However, overnutrition and a steady increase in obesity in the U.S. has made it harder for many mothers to produce and express breastmilk. Moreover, the quality of breastmilk from obese mothers is frequently compromised in that it contains fewer nutrients and more inflammatory components. In this study, we used mice to model this phenomenon. We found that short-term high-fat feeding at the start of breeding reduces litter size and pups’ body weight. It also impairs adipocyte remodeling during lactation. Connexin43 is the primary building block for gap junctions …

Extracellular Domain Of Pept1 Interacts With Tm1 To Facilitate Substrate Transport, Jiemin Shen, Miaohui Hu, Xiao Fan, Zhenning Ren, Corinne Portioli, Xiuwen Yan, Mingqiang Rong, Ming Zhou

Faculty, Staff and Students Publications

Mammalian peptide transporters, PepT1 and PepT2, mediate uptake of small peptides and are essential for their absorption. PepT also mediates absorption of many drugs and prodrugs to enhance their bioavailability. PepT has twelve transmembrane (TM) helices that fold into an N-terminal domain (NTD, TM1–6) and a C-terminal domain (CTD, TM7–12), and has a large extracellular domain (ECD) between TM9–10. It is well-recognized that peptide transport requires movements of the NTD and CTD, but the role of the ECD in PepT1 remains unclear. Here we report the structure of horse PepT1 encircled in lipid nanodiscs and captured in the inward-open apo …

Long-Term Follow-Up For The Development Of Subsequent Malignancies In Patients Treated With Genetically Modified Iecs, David H M Steffin, Ibrahim N Muhsen, Laquisa C Hill, Carlos A Ramos, Nabil Ahmed, Meenakshi Hegde, Tao Wang, Mengfen Wu, Stephen Gottschalk, Sarah B Whittle, Premal D Lulla, Maksim Mamonkin, Bilal Omer, Rayne H Rouce, Andras Heczey, Leonid S Metelitsa, Bambi J Grilley, Catherine Robertson, Virginia Torrano, Natalia Lapteva, Adrian P Gee, Cliona M Rooney, Malcolm K Brenner, Helen E Heslop

Faculty, Staff and Students Publications

Subsequent malignancies are well-documented complications in long-term follow-up of cancer patients. Recently, genetically modified immune effector (IE) cells have shown benefit in hematologic malignancies and are being evaluated in clinical trials for solid tumors. Although the short-term complications of IE cells are well described, there is limited literature summarizing long-term follow-up, including subsequent malignancies. We retrospectively reviewed data from 340 patients treated across 27 investigator-initiated pediatric and adult clinical trials at our center. All patients received IE cells genetically modified with γ-retroviral vectors to treat relapsed and/or refractory hematologic or solid malignancies. In a cumulative 1027 years of long-term follow-up, …

Structure-Activity Relationship And Antitumor Activity Of 1,4-Pyrazine-Containing Inhibitors Of Histone Acetyltransferases P300/Cbp, Shenyou Nie, Fangrui Wu, Jingyu Wu, Xin Li, Chao Zhou, Yuan Yao, Yongcheng Song

Faculty, Staff and Students Publications

Acetylation of histone lysine residues by histone acetyltransferase (HAT) p300 and its paralog CBP play important roles in gene regulation in health and diseases. The HAT domain of p300/CBP has been found to be a potential drug target for cancer. Compound screening followed by structure-activity relationship studies yielded a novel series of 1,4-pyrazine-containing inhibitors of p300/CBP HAT with their IC50s as low as 1.4 μM. Enzyme kinetics and other studies support the most potent compound 29 is a competitive inhibitor of p300 HAT against the substrate histone. It exhibited a high selectivity for p300 and CBP, with negligible activity on …

Multifaceted Intervention To Improve P2y12 Inhibitor Adherence After Percutaneous Coronary Intervention: A Stepped Wedge Trial, P Michael Ho, Colin I O'Donnell, Marina Mccreight, Anthony A Bavry, Hayden B Bosworth, Saket Girotra, P Michael Grossman, Christian Helfrich, Faisal Latif, David Lu, Michael Matheny, Kreton Mavromatis, Jose Ortiz, Amitabh Parashar, Devona M Ratliff, Gary K Grunwald, Michael Gillette, Hani Jneid

Faculty, Staff and Students Publications

Background P2Y12 inhibitor medications are critical following percutaneous coronary intervention (PCI); however, adherence remains suboptimal. Our objective was to assess the effectiveness of a multifaceted intervention to improve P2Y12 inhibitor adherence following PCI. Methods and Results This was a modified stepped wedge trial of 52 eligible hospitals, of which 15 were randomly selected and agreed to participate (29 hospitals declined, and 8 eligible hospitals were not contacted). At each intervention hospital, patient recruitment occurred for 6 months and enrolled patients were followed up for 1 year after PCI. Three control groups were used: patients at intervention hospitals undergoing PCI (1) …

Changing Global Epidemiology Of Liver Cancer From 2010 To 2019: Nash Is The Fastest Growing Cause Of Liver Cancer, Daniel Q Huang, Amit G Singal, Yuko Kono, Darren J H Tan, Hashem B El-Serag, Rohit Loomba

Faculty, Staff and Students Publications

Liver cancer epidemiology is changing due to increasing alcohol consumption, rising prevalence of obesity, and advances in hepatitis B virus (HBV) and hepatitis C virus (HCV) treatment. However, the impact of these changes on global liver cancer burden remains unclear. We estimated global and regional temporal trends in the burden of liver cancer and the contributions of various liver disease etiologies using the methodology framework of the Global Burden of Disease study. Between 2010 and 2019, there was a 25% increase in liver cancer deaths. Age-standardized death rates (ASDRs) increased only in the Americas and remained stable or fell in …

The 5th Edition Of The World Health Organization Classification Of Haematolymphoid Tumours: Myeloid And Histiocytic/Dendritic Neoplasms, Joseph D Khoury, Eric Solary, Oussama Abla, Yassmine Akkari, Rita Alaggio, Jane F Apperley, Rafael Bejar, Emilio Berti, Lambert Busque, John K C Chan, Weina Chen, Xueyan Chen, Wee-Joo Chng, John K Choi, Isabel Colmenero, Sarah E Coupland, Nicholas C P Cross, Daphne De Jong, M Tarek Elghetany, Emiko Takahashi, Jean-Francois Emile, Judith Ferry, Linda Fogelstrand, Michaela Fontenay, Ulrich Germing, Sumeet Gujral, Torsten Haferlach, Claire Harrison, Jennelle C Hodge, Shimin Hu, Joop H Jansen, Rashmi Kanagal-Shamanna, Hagop M Kantarjian, Christian P Kratz, Xiao-Qiu Li, Megan S Lim, Keith Loeb, Sanam Loghavi, Andrea Marcogliese, Soheil Meshinchi, Phillip Michaels, Kikkeri N Naresh, Yasodha Natkunam, Reza Nejati, German Ott, Eric Padron, Keyur P Patel, Nikhil Patkar, Jennifer Picarsic, Uwe Platzbecker, Irene Roberts, Anna Schuh, William Sewell, Reiner Siebert, Prashant Tembhare, Jeffrey Tyner, Srdan Verstovsek, Wei Wang, Brent Wood, Wenbin Xiao, Cecilia Yeung, Andreas Hochhaus

Faculty, Staff and Students Publications

The upcoming 5th edition of the World Health Organization (WHO) Classification of Haematolymphoid Tumours is part of an effort to hierarchically catalogue human cancers arising in various organ systems within a single relational database. This paper summarizes the new WHO classification scheme for myeloid and histiocytic/dendritic neoplasms and provides an overview of the principles and rationale underpinning changes from the prior edition. The definition and diagnosis of disease types continues to be based on multiple clinicopathologic parameters, but with refinement of diagnostic criteria and emphasis on therapeutically and/or prognostically actionable biomarkers. While a genetic basis for defining diseases is sought …

Pilot Phase Results Of A Prospective, Randomized Controlled Trial Of Narrowband Ultraviolet B Phototherapy In Hospitalized Covid-19 Patients, Frank H Lau, Catherine E Powell, Giacomo Adonecchi, Denise M Danos, Andrew R Dinardo, Robert J Chugden, Peter Wolf, Carmen F Castilla

Faculty, Staff and Students Publications

COVID-19 morbidity and mortality are driven by poor immune regulation. Narrowband ultraviolet B (NB-UVB) phototherapy is standard of care in a number of immune-dysregulated diseases. To assess the efficacy of NB-UVB phototherapy for improving COVID-19 outcomes in high-risk, hospitalized, we developed the Adaptive Photo-Protection Trial. This is a multi-center, prospective, double-blinded, randomized, placebo-controlled trial. The pilot phase results are reported here. Consecutive patients admitted with a positive COVID-19 PCR were screened for eligibility. Enrolled subjects were computer randomized 1:1 to NB-UVB or placebo phototherapy. Subjects were treated daily with escalating doses on 27% of their body surface area for up …

White Coat Hypertension Persistence In Children And Adolescents: The Pediatric Nephrology Research Consortium Study, Yosuke Miyashita, Coral Hanevold, Anna Faino, Julia Scher, Marc Lande, Ikuyo Yamaguchi, Joel Hernandez, Alisa Acosta, Donald J Weaver, Jason Thomas, Mahmoud Kallash, Michael Ferguson, Ketan N Patel, Andrew M South, Megan Kelton, Joseph T Flynn

Faculty, Staff and Students Publications

OBJECTIVES: To determine whether youth with white coat hypertension on initial ambulatory blood pressure monitoring (ABPM) continue to demonstrate the same pattern on repeat ABPM.

STUDY DESIGN: Retrospective longitudinal cohort study of patients referred for high blood pressure (BP) and diagnosed with white coat hypertension by ABPM who had follow-up ABPM 0.5-4.6 years later at 11 centers in the Pediatric Nephrology Research Consortium. We classified ABPM phenotype using the American Heart Association guidelines. At baseline, we classified those with hypertensive BP in the clinic as "stable white coat hypertension," and those with normal BP as "intermittent white coat hypertension." We …

Monkeypox: A Contemporary Review For Healthcare Professionals, Boghuma K Titanji, Bryan Tegomoh, Saman Nematollahi, Michael Konomos, Prathit A Kulkarni

Faculty, Staff and Students Publications

The ongoing 2022 multicountry outbreak of monkeypox is the largest in history to occur outside of Africa. Monkeypox is an emerging zoonotic disease that for decades has been viewed as an infectious disease with significant epidemic potential because of the increasing occurrence of human outbreaks in recent years. As public health entities work to contain the current outbreak, healthcare professionals globally are aiming to become familiar with the various clinical presentations and management of this infection. We present in this review an updated overview of monkeypox for healthcare professionals in the context of the ongoing outbreaks around the world.

Socio-Demographic And Trauma-Related Predictors Of Depression Within Eight Weeks Of Motor Vehicle Collision In The Aurora Study, Jutta Joormann, Samuel A Mclean, Francesca L Beaudoin, Xinming An, Jennifer S Stevens, Donglin Zeng, Thomas C Neylan, Gari Clifford, Sarah D Linnstaedt, Laura T Germine, Scott L Rauch, Paul I Musey, Phyllis L Hendry, Sophia Sheikh, Christopher W Jones, Brittany E Punches, Gregory Fermann, Lauren A Hudak, Kamran Mohiuddin, Vishnu Murty, Meghan E Mcgrath, John P Haran, Jose Pascual, Mark Seamon, David A Peak, Claire Pearson, Robert M Domeier, Paulina Sergot, Roland Merchant, Leon D Sanchez, Niels K Rathlev, William F Peacock, Steven E Bruce, Deanna Barch, Diego A Pizzagalli, Beatriz Luna, Steven E Harte, Irving Hwang, Sue Lee, Nancy Sampson, Karestan C Koenen, Kerry J Ressler, Ronald C Kessler

Faculty, Staff and Students Publications

BACKGROUND: This is the first report on the association between trauma exposure and depression from the

METHODS: We focus on participants presenting at EDs after a motor vehicle collision (MVC), which characterizes most AURORA participants, and examine associations of participant socio-demographics and MVC characteristics with 8-week depression as mediated through peritraumatic symptoms and 2-week depression.

RESULTS: Eight-week depression prevalence was relatively high (27.8%) and associated with several MVC characteristics (being passenger

CONCLUSIONS: These observations, coupled with substantial variation in the relative strength of the mediating pathways across predictors, raises the possibility of diverse and potentially complex underlying biological and psychological …

Analysis Of An Antibiotic Stewardship Program For Asymptomatic Bacteriuria In The Veterans Affairs Health Care System, Larissa Grigoryan, Aanand D Naik, Paola Lichtenberger, Christopher J Graber, Payal K Patel, Dimitri M Drekonja, Timothy P Gauthier, Bhavarth Shukla, Anne E Sales, Sarah L Krein, John N Van, Laura M Dillon, Sylvia J Hysong, Jennifer R Kramer, Annette Walder, David Ramsey, Barbara W Trautner

Faculty, Staff and Students Publications

IMPORTANCE: Antibiotic stewardship for asymptomatic bacteriuria (ASB) is an important quality improvement target. Understanding how to implement successful antibiotic stewardship interventions is limited.

OBJECTIVE: To evaluate the effectiveness of a quality improvement stewardship intervention on reducing unnecessary urine cultures and antibiotic use in patients with ASB.

DESIGN, SETTING, AND PARTICIPANTS: This interrupted time series quality improvement study was performed at the acute inpatient medical and long-term care units of 4 intervention sites and 4 comparison sites in the Veterans Affairs (VA) health care system from October 1, 2017, through April 30, 2020. Participants included the clinicians who order or collect …

Detection Accuracy Of The Cobas Hpv Assay For High-Risk Hpv In Head And Neck Fna Biopsy Specimens, Ming Guo, Abha Khanna, Agata A Tinnirello, Jessica Hwang, Ping Zhang, Li Xu, Guojun Li, Kristina R Dahlstrom, Erich M Sturgis, John Stewart

Faculty, Staff and Students Publications

BACKGROUND: This study evaluated the detection accuracy of the Cobas human papillomavirus (HPV) assay for high-risk human papillomavirus (hrHPV) and HPV-16 in head and neck fine-needle aspiration (FNA) specimens with squamous cell carcinoma.

METHODS: Head and neck FNA biopsy specimens from 2012 to 2020 were retrospectively collected. Cobas HPV testing was performed on 90 FNA specimens with valid Cervista HPV testing results. Results of Cobas HPV and Cervista HPV assays were compared. A Linear Array or SPF10-LiPA25 HPV genotyping assay resolved cases with discrepant results. The κ value and accuracy of Cobas HPV testing were calculated. The accuracy of the …

Translating Electronic Health Record-Based Patient Safety Algorithms From Research To Clinical Practice At Multiple Sites, Andrew J Zimolzak, Hardeep Singh, Daniel R Murphy, Li Wei, Sahar A Memon, Divvy K Upadhyay, Saritha Korukonda, Lisa Zubkoff, Dean F Sittig

Faculty, Staff and Students Publications

INTRODUCTION: Researchers are increasingly developing algorithms that impact patient care, but algorithms must also be implemented in practice to improve quality and safety.

OBJECTIVE: We worked with clinical operations personnel at two US health systems to implement algorithms to proactively identify patients without timely follow-up of abnormal test results that warrant diagnostic evaluation for colorectal or lung cancer. We summarise the steps involved and lessons learned.

METHODS: Twelve sites were involved across two health systems. Implementation involved extensive software documentation, frequent communication with sites and local validation of results. Additionally, we used automated edits of existing code to adapt it …

Mir-181a Promotes Multiple Protumorigenic Functions By Targeting Tgfβr3, Vida Chitsazzadeh, Tran N Nguyen, Alvaro De Mingo Pulido, Bruna B Bittencourt, Lili Du, Charles H Adelmann, Ivannie Ortiz Rivera, Kimberly A Nguyen, Leah D Guerra, Andrew Davis, Marco Napoli, Wencai Ma, Richard Eric Davis, Kimal Rajapakshe, Cristian Coarfa, Elsa R Flores, Kenneth Y Tsai

Faculty, Staff and Students Publications

Cutaneous squamous cell carcinoma (cSCC) comprises 15‒20% of all skin cancers and has a well-defined progression sequence from precancerous actinic keratosis to invasive cSCC. To identify targets for chemoprevention, we previously reported a cross-species analysis to identify the transcriptional drivers of cSCC development and identified miR-181a as a potential oncomiR. We show that the upregulation of miR-181a promotes multiple protumorigenic properties by targeting an understudied component of TGFβ signaling, TGFβR3. miR-181a and TGFβR3 are upregulated and downregulated, respectively, in cSCC. miR-181a overexpression (OE) and TGFβR3 knockdown (KD) significantly suppresses UV-induced apoptosis in HaCaT cells and in primary normal human epidermal …

Crispr Detection Of Circulating Cell-Free Mycobacterium Tuberculosis Dna In Adults And Children, Including Children With Hiv: A Molecular Diagnostics Study, Zhen Huang, Sylvia M Lacourse, Alexander W Kay, Joshua Stern, Jaclyn N Escudero, Brady M Youngquist, Wenshu Zheng, Debrah Vambe, Muyalo Dlamini, Godwin Mtetwa, Lisa M Cranmer, Irene Njuguna, Dalton C Wamalwa, Elizabeth Maleche-Obimbo, Donald G Catanzaro, Christopher J Lyon, Grace John-Stewart, Andrew Dinardo, Anna M Mandalakas, Bo Ning, Tony Y Hu

Faculty, Staff and Students Publications

BACKGROUND: Tuberculosis remains a leading cause of global mortality, especially for adults and children living with HIV (CLHIV) underdiagnosed by sputum-based assays. Non-sputum-based assays are needed to improve tuberculosis diagnosis and tuberculosis treatment monitoring. Our aim in this study was to determine whether ultrasensitive detection of Mycobacterium tuberculosis cell-free DNA (Mtb-cfDNA) in blood can diagnose tuberculosis and evaluate tuberculosis treatment responses.

METHODS: In this molecular diagnostics study we analysed archived serum from two patient populations evaluated for tuberculosis in Eswatini and Kenya to detect Mtb-cfDNA, analysing serum from all individuals who had both sufficient serum volumes and clear diagnostic results. …

Accelerated Identification Of Disease-Causing Variants With Ultra-Rapid Nanopore Genome Sequencing, Sneha D Goenka, John E Gorzynski, Kishwar Shafin, Dianna G Fisk, Trevor Pesout, Tanner D Jensen, Jean Monlong, Pi-Chuan Chang, Gunjan Baid, Jonathan A Bernstein, Jeffrey W Christle, Karen P Dalton, Daniel R Garalde, Megan E Grove, Joseph Guillory, Alexey Kolesnikov, Maria Nattestad, Maura R Z Ruzhnikov, Mehrzad Samadi, Ankit Sethia, Elizabeth Spiteri, Christopher J Wright, Katherine Xiong, Tong Zhu, Miten Jain, Fritz J Sedlazeck, Andrew Carroll, Benedict Paten, Euan A Ashley

Faculty, Staff and Students Publications

Whole-genome sequencing (WGS) can identify variants that cause genetic disease, but the time required for sequencing and analysis has been a barrier to its use in acutely ill patients. In the present study, we develop an approach for ultra-rapid nanopore WGS that combines an optimized sample preparation protocol, distributing sequencing over 48 flow cells, near real-time base calling and alignment, accelerated variant calling and fast variant filtration for efficient manual review. Application to two example clinical cases identified a candidate variant inprioritization, and accelerates diagnostic clinical genome sequencing twofold compared with previous approaches.

Spatial Analysis Of Drug Absorption, Distribution, Metabolism, And Toxicology Using Mass Spectrometry Imaging, Michelle L Spruill, Mirjana Maletic-Savatic, Howard Martin, Feng Li, Xinli Liu

Faculty, Staff and Students Publications

Mass spectrometry imaging (MSI) is emerging as a powerful analytical tool for detection, quantification, and simultaneous spatial molecular imaging of endogenous and exogenous molecules via in situ mass spectrometry analysis of thin tissue sections without the requirement of chemical labeling. The MSI generates chemically specific and spatially resolved ion distribution information for administered drugs and metabolites, which allows numerous applications for studies involving various stages of drug absorption, distribution, metabolism, excretion, and toxicity (ADMET). MSI-based pharmacokinetic imaging analysis provides a histological context and cellular environment regarding dynamic drug distribution and metabolism processes, and facilitates the understanding of the spatial pharmacokinetics …

Association Of Rare Apoe Missense Variants V236e And R251g With Risk Of Alzheimer Disease, Yann Le Guen, Michael E Belloy, Benjamin Grenier-Boley, Itziar De Rojas, Atahualpa Castillo-Morales, Iris Jansen, Aude Nicolas, Céline Bellenguez, Carolina Dalmasso, Fahri Küçükali, Sarah J Eger, Katrine Laura Rasmussen, Jesper Qvist Thomassen, Jean-François Deleuze, Zihuai He, Valerio Napolioni, Philippe Amouyel, Frank Jessen, Patrick G Kehoe, Cornelia Van Duijn, Magda Tsolaki, Pascual Sánchez-Juan, Kristel Sleegers, Martin Ingelsson, Giacomina Rossi, Mikko Hiltunen, Rebecca Sims, Wiesje M Van Der Flier, Alfredo Ramirez, Ole A Andreassen, Ruth Frikke-Schmidt, Julie Williams, Agustín Ruiz, Jean-Charles Lambert, Michael D Greicius, Members Of The Eadb, Gr@Ace, Degesco, Demgene, Gerad, And Eadi Groups, Beatrice Arosio, Luisa Benussi, Anne Boland, Barbara Borroni, Paolo Caffarra, Delphine Daian, Antonio Daniele, Stéphanie Debette, Carole Dufouil, Emrah Düzel, Daniela Galimberti, Vilmantas Giedraitis, Timo Grimmer, Caroline Graff, Edna Grünblatt, Olivier Hanon, Lucrezia Hausner, Stefanie Heilmann-Heimbach, Henne Holstege, Jakub Hort, Deckert Jürgen, Teemu Kuulasmaa, Aad Van Der Lugt, Carlo Masullo, Patrizia Mecocci, Shima Mehrabian, Alexandre De Mendonça, Susanne Moebus, Benedetta Nacmias, Gael Nicolas, Robert Olaso, Goran Papenberg, Lucilla Parnetti, Florence Pasquier, Oliver Peters, Yolande A L Pijnenburg, Julius Popp, Innocenzo Rainero, Inez Ramakers, Steffi Riedel-Heller, Nikolaos Scarmeas, Philip Scheltens, Norbert Scherbaum, Anja Schneider, Davide Seripa, Hilkka Soininen, Vincenzo Solfrizzi, Gianfranco Spalletta, Alessio Squassina, John Van Swieten, Thomas J Tegos, Lucio Tremolizzo, Frans Verhey, Martin Vyhnalek, Jens Wiltfang, Mercè Boada, Pablo García-González, Raquel Puerta, Luis M Real, Victoria Álvarez, María J Bullido, Jordi Clarimon, José María García-Alberca, Pablo Mir, Fermin Moreno, Pau Pastor, Gerard Piñol-Ripoll, Laura Molina-Porcel, Jordi Pérez-Tur, Eloy Rodríguez-Rodríguez, Jose Luís Royo, Raquel Sánchez-Valle, Martin Dichgans, Dan Rujescu

Faculty, Staff and Students Publications

IMPORTANCE: The APOE ε2 and APOE ε4 alleles are the strongest protective and risk-increasing, respectively, genetic variants for late-onset Alzheimer disease (AD). However, the mechanisms linking APOE to AD-particularly the apoE protein's role in AD pathogenesis and how this is affected by APOE variants-remain poorly understood. Identifying missense variants in addition to APOE ε2 and APOE ε4 could provide critical new insights, but given the low frequency of additional missense variants, AD genetic cohorts have previously been too small to interrogate this question robustly.

OBJECTIVE: To determine whether rare missense variants on APOE are associated with AD risk.

DESIGN, SETTING, …

Postprandial Symptoms In Patients With Symptoms Of Gastroparesis: Roles Of Gastric Emptying And Accommodation, Henry P Parkman, Mark L Van Natta, Alan H Maurer, Kenneth L Koch, Madhusudan Grover, Zubair Malik, Irene Sarosiek, Thomas L Abell, Robert Bulat, Braden Kuo, Robert J Shulman, Gianrico Farrugia, Laura Miriel, James Tonascia, Frank Hamilton, Pankaj J Pasricha, Richard W Mccallum

Faculty, Staff and Students Publications

Patients often are evaluated for gastroparesis because of symptoms occurring with meals. Gastric emptying scintigraphy (GES) is used for gastroparesis diagnosis, although results are not well correlated with gastroparesis symptoms. The aim of this study is to assess relationships between gastroparesis symptoms, gastric emptying (GE), and gastric accommodation (GA). Patients with symptoms of gastroparesis completed the Patient Assessment of Upper GI Symptoms (PAGI-SYM) and recorded symptoms during GES and water load satiety test (WLST), an indirect assessment for GA. A total of 109 patients with gastroparesis symptoms were assessed. Symptom severity increased after GES meal for stomach fullness, belching, nausea, …

A Role For Insulin-Like Growth Factor 1 In The Generation Of Epileptic Spasms In A Murine Model, Carlos J Ballester-Rosado, John T Le, Trang T Lam, Carrie A Mohila, Sandi Lam, Anne E Anderson, James D Frost, John W Swann

Faculty, Staff and Students Publications

Objective: Infantile spasms are associated with a wide variety of clinical conditions, including perinatal brain injuries. We have created a model in which prolonged infusion of tetrodotoxin (TTX) into the neocortex, beginning in infancy, produces a localized lesion and reproduces the behavioral spasms, electroencephalogram (EEG) abnormalities, and drug responsiveness seen clinically. Here, we undertook experiments to explore the possibility that the growth factor IGF-1 plays a role in generating epileptic spasms.

Methods: We combined long-term video EEG recordings with quantitative immunohistochemical and biochemical analyses to unravel IGF-1's role in spasm generation. Immunohistochemistry was undertaken in surgically resected tissue from infantile …

Activating Connexin43 Gap Junctions Primes Adipose Tissue For Therapeutic Intervention, Yi Zhu, Na Li, Mingyang Huang, Xi Chen, Yu A An, Jianping Li, Shangang Zhao, Jan-Bernd Funcke, Jianhong Cao, Zhenyan He, Qingzhang Zhu, Zhuzhen Zhang, Zhao V Wang, Lin Xu, Kevin W Williams, Chien Li, Kevin Grove, Philipp E Scherer

Faculty, Staff and Students Publications

Adipose tissue is a promising target for treating obesity and metabolic diseases. However, pharmacological agents usually fail to effectively engage adipocytes due to their extraordinarily large size and insufficient vascularization, especially in obese subjects. We have previously shown that during cold exposure, connexin43 (Cx43) gap junctions are induced and activated to connect neighboring adipocytes to share limited sympathetic neuronal input amongst multiple cells. We reason the same mechanism may be leveraged to improve the efficacy of various pharmacological agents that target adipose tissue. Using an adipose tissue-specific Cx43 overexpression mouse model, we demonstrate effectiveness in connecting adipocytes to augment metabolic …

Innovation And Missed Opportunities In Internal Medicine Undergraduate Education During Covid-19: Results From A National Survey, Irene Alexandraki, Katherine J Walsh, Temple Ratcliffe, Chavon Onumah, Karen Szauter, Camilla Curren, Nora Osman, Cindy J Lai, Deborah Dewaay, Nicholas S Duca, Amy Weinstein, Nadia Ismail, Jackcy Jacob, Michael Kisielewski, Amber T Pincavage

Faculty, Staff and Students Publications

BACKGROUND: COVID-19 disrupted undergraduate clinical education when medical schools removed students from clinical rotations following AAMC recommendations. Clerkship directors (CDs) had to adapt rapidly and modify clerkship curricula. However, the scope and effects of these modifications are unknown.

OBJECTIVE: To examine the effects of the initial phase of COVID-19 on the internal medicine (IM) undergraduate clinical education.

DESIGN: A nationally representative web survey.

PARTICIPANTS: IM CDs from 137 LCME-accredited US medical schools in 2020.

MAIN MEASURES: Items (80) assessed clerkship structure and curriculum, assessment in clerkships, post-clerkship IM clinical experiences, and CD roles and support. The framework of Understanding Crisis …

Chronic Lung Allograft Dysfunction, Anupam Kumar, Puneet Singh Garcha

Faculty, Staff and Students Publications

Lung transplantation remains the only cure for selected patients with advanced irreversible lung diseases. More than 4000 lung transplants are performed worldwide annually. In the last two decades, significant advances have been made in this arena, the most impactful being a modest but improved survival of the recipients. Unfortunately majority of recipients still succumb to chronic lung allograft dysfunction (CLAD), and it remains the most common cause of death after the first year of transplantation. Below is a concise review of the current definition of CLAD, including the various phenotypes, and a brief discussion of the tools available for its …