Medical Specialties Commons^™

Platelet Glycogenolysis Is Important For Energy Production And Function, Kanakanagavalli Shravani Prakhya, Hemendra J. Vekaria, Daniёlle M. Coenen, Linda Omali, Joshua Lykins, Smita Joshi, Hammodah Rawhi Hammodah Alfar, Qing Jun Wang, Patrick G. Sullivan, Sidney W. Whiteheart

Saha Cardiovascular Research Center Faculty Publications

Although the presence of glycogen in platelets was established in the 1960s, its importance to specific functions (i.e., activation, secretion, aggregation, and clot contraction) remains unclear. Patients with glycogen storage disease often present with increased bleeding and glycogen phosphorylase (GP) inhibitors, when used as treatments for diabetes, induce bleeding in preclinical studies suggesting some role for this form of glucose in hemostasis. In the present work, we examined how glycogen mobilization affects platelet function using GP inhibitors (CP316819 and CP91149) and a battery of ex vivo assays. Blocking GP activity increased glycogen levels in resting and thrombin-activated platelets and inhibited …

The Complementary Roles Of Vamp-2, -3, And -7 In Platelet Secretion And Function, Smita Joshi, Kanakanagavalli Shravani Prakhya, Alexis N. Smith, Harry Chanzu, Ming Zhang, Sidney W. Whiteheart

Saha Cardiovascular Research Center Faculty Publications

Platelet secretion requires Soluble N-ethylmaleimide Sensitive Attachment Protein Receptors (SNAREs). Vesicle SNAREs/Vesicle-Associated Membrane Proteins (v-SNAREs/ VAMPs) on granules and t-SNAREs in plasma membranes mediate granule release. Platelet VAMP heterogeneity has complicated the assessment of how/if each is used and affects hemostasis. To address the importance of VAMP-7 (V7), we analyzed mice with global deletions of V3 and V7 together or platelet-specific deletions of V2, V3, and global deletion of V7. We measured the kinetics of cargo release, and its effects on three injury models to define the context-specific roles of these VAMPs. Loss of V7 minimally affected dense and α …

Best3-Mediated Mekk2/3 Activation: A Novel Therapeutic Target In Aortopathies, Tatiana Zyrianova, Benjamin Lopez, Kathlyn Zou, Charles Gu, Dayna Pham, Sriharsha Talapaneni, Christopher M. Waters, Riccardo Olcese, Andreas Schwingshackl

Saha Cardiovascular Research Center Faculty Publications

Influenza-A virus (IAV) infects yearly an estimated one billion people worldwide, resulting in 300,000–650,000 deaths. Preventive vaccination programs and antiviral medications represent the mainstay of therapy, but with unacceptably high morbidity and mortality rates, new targeted therapeutic approaches are urgently needed. Since inflammatory processes are commonly associated with measurable changes in the cell membrane potential (Em), we investigated whether Em hyperpolarization via TREK-1 (K2P 2.1) K þ channel activation can protect against influenza-A virus (IAV)-induced pneumonia. We infected mice with IAV, which after 5 days caused 10–15% weight loss and a decrease in spontaneous activity, representing a clinically relevant infection. …

Deep Learning, 3d Ultrastructural Analysis Reveals Quantitative Differences In Platelet And Organelle Packing In Covid-19/Sarscov2 Patient-Derived Platelets, Sagar S. Matharu, Cassidy S. Nordmann, Kurtis R. Ottman, Rahul Akkem, Douglas Palumbo, Denzel R. D. Cruz, Kenneth Campbell, Gail Sievert, Jamie L. Sturgill, James Zachary Porterfield, Smita Joshi, Hammodah Rawhi Hammodah Alfar, Chi Peng, Irina D. Pokrovskaya, Jeffrey A. Kamykowski, Jeremy P. Wood, Beth A. Garvy, Maria A. Aronova, Sidney W. Whiteheart, Richard D. Leapman, Brian Storrie

Saha Cardiovascular Research Center Faculty Publications

Platelets contribute to COVID-19 clinical manifestations, of which microclotting in the pulmonary vasculature has been a prominent symptom. To investigate the potential diagnostic contributions of overall platelet morphology and their α-granules and mitochondria to the understanding of platelet hyperactivation and micro-clotting, we undertook a 3D ultrastructural approach. Because differences might be small, we used the high-contrast, high-resolution technique of focused ion beam scanning EM (FIB-SEM) and employed deep learning computational methods to evaluate nearly 600 individual platelets and 30 000 included organelles within three healthy controls and three severely ill COVID-19 patients. Statistical analysis reveals that the α-granule/ mitochondrion-to-plateletvolume ratio …

Lrp1 Protects Against Excessive Superior Mesenteric Artery Remodeling By Modulating Angiotensin Ii–Mediated Signaling, Jackie M. Zhang, Dianaly T. Au, Hisashi Sawada, Michael K. Franklin, Jessica J. Moorleghen, Deborah A. Howatt, Pengjun Wang, Brittany O. Aicher, Brian Hampton, Mary Migliorini, Fenge Ni, Adam E. Mullick, Mashhood M. Wani, Areck A. Ucuzian, Hong S. Lu, Selen C. Muratoglu, Alan Daugherty, Dudley K. Strickland

Saha Cardiovascular Research Center Faculty Publications

Vascular smooth muscle cells (vSMCs) exert a critical role in sensing and maintaining vascular integrity. These cells abundantly express the low-density lipoprotein receptor–related protein 1 (LRP1), a large endocytic signaling receptor that recognizes numerous ligands, including apolipoprotein E–rich lipoproteins, proteases, and protease-inhibitor complexes. We observed the spontaneous formation of aneurysms in the superior mesenteric artery (SMA) of both male and female mice in which LRP1 was genetically deleted in vSMCs (smLRP1–/– mice). Quantitative proteomics revealed elevated abundance of several proteins in smLRP1–/– mice that are known to be induced by angiotensin II–mediated (AngII-mediated) signaling, suggesting that this pathway was dysregulated. …

Macrophage Angiotensin-Converting Enzyme Reduces Atherosclerosis By Increasing Peroxisome Proliferator-Activated Receptor Α And Fundamentally Changing Lipid Metabolism, Duoyao Cao, Zakir Khan, Xiaomo Li, Suguru Saito, Ellen A. Bernstein, Aaron R. Victor, Faizan Ahmed, Aoi O. Hoshi, Luciana C. Veiras, Tomohiro Shibata, Mingtian Che, Lei Cai, Michifumi Yamashita, Ryan E. Temel, Jorge F. Giani, Daniel J. Luthringer, Ajit S. Divakaruni, Derick Okwan-Duodu, Kenneth E. Bernstein

Saha Cardiovascular Research Center Faculty Publications

Aims The metabolic failure of macrophages to adequately process lipid is central to the aetiology of atherosclerosis. Here, we examine the role of macrophage angiotensin-converting enzyme (ACE) in a mouse model of PCSK9-induced atherosclerosis.

Methods and results Atherosclerosis in mice was induced with AAV-PCSK9 and a high-fat diet. Animals with increased macrophage ACE (ACE 10/10 mice) have a marked reduction in atherosclerosis vs. WT mice. Macrophages from both the aorta and peritoneum of ACE 10/10 express increased PPARα and have a profoundly altered phenotype to process lipids characterized by higher levels of the surface scavenger receptor CD36, increased uptake of …

Histoplasma Tympanomastoiditis: Case Report And Literature Review, Terrence Park, Gordon Love, Victoria Burke

School of Medicine Faculty Publications

Histoplasma is a dimorphic fungus capable of producing a diverse array of clinical pathology in humans dependent upon the host immune status. Acute symptomatic infection typically presents as an isolated pulmonary or nodal disease in immunocompetent patients with extra-thoracic manifestations rarely seen in this population. In this report, we describe a novel case of Histoplasma capsulatum tympanomastoiditis in an immunocompetent patient who presented with progressively worsening purulent otorrhea, vertigo, and facial nerve palsy. He was successfully managed with surgical debridement and a prolonged antifungal course.

The Antimicrobial Peptide Database Is 20 Years Old: Recent Developments And Future Directions, Guangshun Wang

Journal Articles: Pathology and Microbiology

In 2023, the Antimicrobial Peptide Database (currently available at https://aps.unmc.edu) is 20-years-old. The timeline for the APD expansion in peptide entries, classification methods, search functions, post-translational modifications, binding targets, and mechanisms of action of antimicrobial peptides (AMPs) has been summarized in our previous Protein Science paper. This article highlights new database additions and findings. To facilitate antimicrobial development to combat drug-resistant pathogens, the APD has been re-annotating the data for antibacterial activity (active, inactive, and uncertain), toxicity (hemolytic and nonhemolytic AMPs), and salt tolerance (salt sensitive and insensitive). Comparison of the respective desired and undesired AMP groups produces new knowledge …

Dietary Whey Protein Increases Brain Leukocytes In Mice Regardless Of Their Hypersensitivity Status, Dilini Ekanayake

Biomedical Sciences Posters and Presentations

Cow’s milk allergy (CMA) often manifests as milder reactions and may be linked to neurological problems. Previously, we demonstrated that C57BL/6J mice sensitized to a bovine whey allergen, β-lactoglobulin (BLG, Bos d 5), moderately increased BLG-specific IgE levels and exhibited behavioral changes without severe allergic reactions. When these non-anaphylactic CMA mice were placed on a whey-protein (WP)-containing diet for 2 weeks to simulate continuous dairy consumption, we found neuropathology indicative of neuroinflammation and cortical demyelination. Since immune cells migrate to the central nervous system (CNS) and promote neuroinflammation in demyelinating conditions such as multiple sclerosis, we hypothesized that the number …

Investigating The Potential Role Of Akkermansia Muciniphila Supplementation In Neuroinflammation: A Progress Report, Geetika Verma

Biomedical Sciences Posters and Presentations

Gut bacteria are important for proper development and function of the host immune system (1). Recent studies have provided association of altered microbiome in both food allergy and neuropsychiatric disorders (2, 3) indicating potential role of microbiome in regulating Gut-Brain-Axis. Akkermansia muciniphila belongs to the phylum Verrucomicrobia, is a commensal mucin degrading bacterial species (4). A. muciniphila protects the gut barrier by facilitating host mucus production. Since patients with food allergy have increased gut permeability, protection of intestinal barrier by increased mucus production may be beneficial in preventing allergen and pathogen infiltrations, hence, minimizing inflammation. A. muciniphila is well known …

A Comparative Study Of Clinical Profile Of Hospitalized Covid-19 Patients In Relation To Their Vaccination Status, Nisha Kaithwas, Garima Agrawal Varshney, Mohit Garg, Piyush Mishra Statistician, Soumitra Sethia

Journal of Medicine in Scientific Research

Background: The clinical picture, outcome of Covid-19 infection and vaccine effectiveness vary in different individuals. These may depend on multiple variables like age, sex, comorbidities, and immune status against the virus and in general. Objective: To describe demography, clinical profile, vaccination status and its impact on clinical outcome of hospitalized Covid-19 patients. Methods: This retrospective study was conducted on hospitalized Covid-19 patients of N.S.C.G.M.C. Khandwa, a tertiary care centre from central India. We studied 188 Covid-19 positive admitted patients’ demographic profile, clinical profile, co-morbidity, vaccination status, clinical outcome and association of these variables to their vaccination status during second Covid-19 …

Clinical Findings And Hematological Parameters In Cases Of Acute Lymphoblastic Leukemia With Thrombocytosis At Initial Diagnosis, Anurag Singh, Sanjay Mishra, Tanya Tripathi, Akanksha Singh, Rashmi Kushwaha, S.P. Verma, U.S. Singh

Journal of Medicine in Scientific Research

Background Acute lymphoblastic leukemia (ALL) is a bone marrow and blood neoplasm involving precursor cells, or lymphoblasts, dedicated to B/T-cell lineages. ALL usually presents with bicytopenia/pancytopenia and thrombocytopenia is the most important key parameter. Thrombocytosis has not been a well-described feature of ALL; hence, this study was planned to investigate the incidence of thrombocytosis at initial diagnosis and its correlation with clinicohematological parameters, immunophenotyping, and cytogenetic study. Aim The aim was to investigate the incidence of thrombocytosis in newly diagnosed cases of ALL and its correlation with clinicohematological parameters, immunophenotyping, and cytogenetics. Materials and methods Medical records of all patients …

Forced Expiratory Volume In The 6 S And Forced Expiratory Volume In The 1 S/Forced Expiratory Volume In The 6 S For Early Detection Of Airflow Obstruction, Therese S. Ghatas, Moheb W. Elfaizy

Journal of Medicine in Scientific Research

Introduction

Use of affordable and simple tools for screening chronic obstructive pulmonary disease (COPD) is limited. In recent years, measurement of forced expiratory volume in the 6 s (FEV6) has been researched as a substitute to replace forced vital capacity measurements. This work was performed to analyze the effectiveness of a simplified handheld forced expiratory flowmeter device and to search an optimal cutoff value of FEV1/FEV6 to detect airflow narrowing and limitation.

Patients and methods

This was a cross-section study for screening and finding of active case among undiagnosed patients with COPD using a simple handheld spirometry device, and the …

Comparison Of Loss Of Resistance Syringe Technique Versus Balloon Technique For Identification Of Epidural Space: A Prospective, Randomized Controlled Study, Isha Singha, Gayatri Vadaratti, R.D. Patel

Journal of Medicine in Scientific Research

Context Epidural anaesthesia has an important role in modern anaesthetic practice due to its safety and efficacy, and its success depends on the technique used for identification of epidural space. Aims: In this study we compared the of loss of resistance (LOR) technique versus the balloon technique for identification of the epidural space in terms of number of attempts and time taken. Also the efficacy of the two techniques in the hands of experienced and inexperienced anaesthesiologists was compared and complications noted. Settings and design Tertiary care hospital. A prospective randomized controlled study. Methods and material 164 ASA I and …

Comparative Assessment Of The Efficacy Of Onabotulinumtoxina As Monotherapy And Combined Therapy With Propiverine In Female Refractory Overactive-Bladder Syndrome, Abourjila A. Gamal Eldin, Ayman M. Gabr, Amr M. Elsofy

Journal of Medicine in Scientific Research

Objectives The aim of the study was to compare the efficacy of onabotulinumtoxinA and anticholinergic popeverine in adult females. This is a placebo-controlled study. Introduction The active ingredient, propiverine hydrochloride, blocks muscarinic receptors with a slightly higher affinity for the M3 as compared with the M2 subtype. In addition, it inhibits calcium influx and modulates intracellular calcium in the bladder smooth-muscle cells, which then leads to musculotropic spasmolysis. Patients and methods Between 2015 and 2019, 94 patients received intravesical onabotulinumtoxinA for the treatment of refractory idiopathic detrusor overactivity after its introduction into the Department of Urology of our National Institute …

Vestibular Assessment In Patients With Temporomandibular Joint Dysfunction Attending Otolaryngology And Oral Medicine Outpatient Clinics, Sanaa Mahran Mohamed Andeil, Zienab Shalaby Farid Shalaby, Anwar Moawad Elbatawy

Journal of Medicine in Scientific Research

Introduction: Temporomandibular joint dysfunction (TMD) is caused by inflammation of the ligaments and muscles surrounding the joints. TMD is associated with several comorbidities, one of these comorbidities are otological problems. The otological symptoms related to TMD are tinnitus, otalgia, ear fullness, and Vertigo. Vertigo is observed in many diseases and etiologic factors should be investigated to increase the efficiency of treatment Objective Here we assess VNG and cVEMP findings in patients with TMD via objective vestibular testing tools including VNG and cVEMP recordings. Here, we compare findings of cervical vestibular evoked myogenic potentials (cVEMP) and of the caloric test, to …

Incidence And Severity Of Pulmonary Hypertension Among Patients With Chronic Kidney Disease, Mamdouh Maher

Journal of Medicine in Scientific Research

Chronic kidney disease (CKD) is a major health problem and is closely linked with cardiovascular disease. Pulmonary hypertension (PH) is considered a common comorbidity in CKD patients. In all, 100 patients were included in our study. Transthoracic echo Doppler study was performed to measure pulmonary artery systolic pressure based on tricuspid jet. The prevalence of PH in CKD patients in our study was 60%; prevalence and severity had significant association.

The hemodialyzed group showed a higher prevalence of PH with a more severe PH.

Our study had provided evidence that PH is a common comorbidity in CKD patients and is …

The Predictive Ability Of Interleukin-6 For Infection After Major Surgery, Naser T. Elshamy, Salama M. Elshennawy, Mahmoud A. Donia

Journal of Medicine in Scientific Research

Objective The aim was to evaluate the role of interleukin (IL)-6 in prediction of infection after major surgeries. Background An early sensitive and specific marker for postoperative infection would be of interest. The cytokines such as IL-6 and IL-10 have been shown to relate to the severity of postoperative sepsis and patient outcome. Patients and methods The study was conducted prospectively at Shebin Elkom Teaching hospital in the period from January 2015 to October 2016 on 50 consecutive patients undergoing elective major surgical procedures. Septic complications were recorded for 4 days postoperatively. For evaluation, patients were allocated into two groups …

Auditory Temporal Processing Abilities In Patients With Diabetes Mellitus Type 1 And Type 2, Abir Omara, Sanaa Mahran, Heba Ramadan

Journal of Medicine in Scientific Research

Introduction: Diabetes mellitus is most common chronic disease characteristic by carbohydrate metabolic disorder in which absolute or relative deficiency in insulin and various pathological changes in the body system. The central nervous system disorders is among system affected by toxic effect and hyperglycemia. Auditory temporal processing is one of the important abilities of the central nervous system which may be affected by diabetes. However, the extent and nature of the central manifestations are still unknown. Objective: The aim of this study was to compare auditory temporal processing in these patients (Diabetics type 1 and Type 2) with normal subjects through …

Prediction Of Response To Disease-Modifying Therapy In Multiple Sclerosis, Abdelrahman M. Abdalla, Azza Abd El-Nasser, Mohamed Fouad, Mahmoud S. Swelam

Journal of Medicine in Scientific Research

Background Multiple sclerosis is an idiopathic inflammatory disease of the central nervous system and the second most common cause of disability in young adults. Choosing an effective treatment is crucial to preventing disability. Aim To identify predictors of disease-modifying therapy (DMT) after 1 year of treatment. Patients and methods In this retrospective study, 150 patients with confirmed diagnosis of relapsing-remittent multiple sclerosis were recruited from the MS Unit at the Neurology Departments from both Ain-Shams University and Cairo University Student Hospital. All of the study population received either interferons or fingolimod. Modified Rio score was used to classify patients to …

An Analytical Study Of The Risk Factors Affecting The Platelet Aggregation In Patients With Sepsis, Vishnu Priya, Jitender M. Khunger, Anupma Arora

Journal of Medicine in Scientific Research

Objectives (a) To determine the correlation of platelet count and aggregation with sepsis severity and (b) to find the risk factors affecting the platelet aggregation in sepsis. Patients and methods The present observational cross-sectional study was conducted on 30 patients of sepsis over a period of 18 months. All patients underwent complete blood counts, and platelet aggregation to ADP. Pearson correlation coefficient was used to assess the association of Sequential Organ Failure Assessment (SOFA) score and aggregation to ADP and platelet. Linear regression was done to determine the risk factors affecting platelet aggregation in sepsis. A P value of less …

Comparative Study Between Radiofrequency Ablation And Endovenous Laser Ablation In The Management Of Primary Great Saphenous Varicose Veins, E.M Reem, H.T Ahmed, A.A Mohammed, H.A Mohamed

Journal of Medicine in Scientific Research

Background: Varicose veins are a common presentation of chronic venous disease. Multiple risk factors have been specified by epidemiological studies such as female sex, pregnancy, obesity, and prior deep venous thrombosis. Objective This is a prospective, comparative study to compare radiofrequency (RF) ablation versus endovenous laser ablation (EVLA) in the management of primary varicose veins of great saphenous vein (GSV). Patients and methods This study was conducted to 130 patients who had primary great saphenous varicose veins who attended to the vascular department of Ain Shams University Hospitals and El-Sahel Teaching Hospital. The study is a prospective comparative clinical trial. …

A Comparison Of Robotically Assisted Needle Guidance Via The Piga Ct System And Conventional Ct For Percutaneous Lung Biopsies, Virender Malik, K Uday Bhanu, Dhanalakshmi B

Journal of Medicine in Scientific Research

Objective

The aim was to compare the speed, radiation exposure, needle adjustments, diagnostic efficacy, and complication rate using manual guidance and robotically assisted needle guidance via the PIGA computed tomography (CT) system for percutaneous lung biopsy.

Methods

A randomized interventional trial was conducted from September 2020 to March 2021 on 30 patients requiring lung biopsies for suspected malignancy. Random allocation of the 30 patients was done in group A (robot-assisted CT scan) and group B (conventional manual CT scan). The outcomes were compared with respect to the total procedural duration, time taken in the navigation of needle insertion, needle manipulations, …

Histomorphological Spectrum Of Ovarian Neoplasms In A Tertiary Care Centre, Lionel Rohit Mathew, Shalini Karthikeyan, Jaishree Tamilmani, Prema Devi Elangovan, Sridevi Vijayasankar

Journal of Medicine in Scientific Research

Introduction: Ovary is the second most common site of gynaecological malignancies in India and therefore ovarian tumours need to be studied under the microscope for accurate diagnosis and management.

Aim: This study aims to study the spectrum of ovarian tumours and calculate their incidence and age-distribution. Materials and Methods: This study was conducted in the Department of Pathology, Sri Muthukumaran Medical College, Hospital & Research Institute, Chennai, during the year 2022. Results: Among a total of 68 ovarian neoplasms studied, benign tumours (61.7%) were more common and most of the patients were in the fourth decade of life. The commonest …

Outcome Of Suture Annuloplasty (De Vega) Versus Ring Annuloplasty For Functional Tricuspid Valve Regurgitation Concomintant With Mitral Valve Surgery, Tarek Elshazly Omran, Ashraf Zahra

Journal of Medicine in Scientific Research

Background: The optimal surgical technique for functional tricuspid regurgitation (TR) repair is still a topic for ongoing research. The DeVega technique is still used frequently in developing countries. Thus, this study aimed to compare the outcomes of tricuspid valve repair using DeVega vs. ring annuloplasty for managing severe functional TR during mitral valve surgery. Methods: This retrospective chart review included 66 patients. The study included patients with simultaneous tricuspid valve repair during mitral valve intervention. The patients were recruited from 2018 to 2022. The study had two groups: the first group included 50 patients who underwent the DeVega technique (Group …

Comparative Study Between Torsional Phacoemulsification And Longitudinal Phacoemulsification In Their Effect On Corneal Endothelium Using Specular Microscopy., Tarek Ibrahim Badr

Journal of Medicine in Scientific Research

Background The cornea is formed of 5 layers, Endothelium is that most posterior layer which is responsible for keeping cornea dehydrated. Loss of corneal endothelium is a sideeffect of many cataract surgeries that may influence postoperative visual outcome of the patients. Our study aims to compare torsional phacoemulsification and longitudinal phacoemulsification in their effect on corneal endothelium using specular microscopy. Patients and Methods We will compare the corneal endothelium parameters (central density of cells & cell loss, alterations in shape & size) afterward torsional phacoemulsification and longitudinal phacoemulsification in this prospective randomized study. 50 eyes with nuclear cataracts in grades …

Natural Cycle Versus Stimulated Or Artificial Cycle In Frozen–Thawed Embryo Transfer: A Randomized Prospective Trial, Salwa H.B. El-Wakil, Naglaa M. Moharam, Ahmed G.A. El-Seuror

Journal of Medicine in Scientific Research

Objective The goal of this study was to see if there was a difference in pregnancy outcomes between natural cycle frozen–thawed embryo transfer (NC-FET) cycles, artificial cycle–FET cycles, and stimulated cycle (SC)–FET cycles in women with regular menstrual cycles. Patients and methods This prospective randomized clinical study was conducted in Assisted Reproductive Unit (ART), The International Islamic Center for Population Studies and Research, El Hussein Hospital, Al-Azhar University, in the period of September 2019 till August 2020. Results There was a significant increase in immotile sperms in the natural group (P=0.014). Group I had much lower total count and total …

Yersinia Infection In Acute Appendicitis In Children In Shebin Elkom Teaching Hospital, Salama M. El- Shennawy, Khaled S.A. Elkelany, Mariam A. Fouad, Shimaa M.M Bebars, Faisel Goda, Abdelghany Abdelsattar Aish

Journal of Medicine in Scientific Research

Background: Yersinia enterocolotica is being recognized as an important bacterial cause of acute gastrointestinal infection. The association of yersinia entrocolotica infections with acute appendicitis has been suggested (Tae Joon Son et. al, 2009). The aim to ascertain whether yersinia entrocolotica is a possible infectious agent in appendicitis in Shebin Elkom. We try to support or to deny this suggestion. Methods: A prospective randomized study was done to 48 patients admitted to surgical department from outpatient clinic of Shebin Elkom Teaching Hospital over 14 months from January 2020 to March 2021The purpose and nature of the study were explained to all …

Should Near Systemic Pulmonary Hypertension Be A Contraindication For Rheumatic Mitral Stenosis Valve Surgery In Egyptian Population?, Ahmed Mostafa Omran, Hany Fayed

Journal of Medicine in Scientific Research

Background: pulmonary hypertension is a well known risk factor leading to mortality and morbidity in Mitral stenosis patients, in spite of long term outcome are being comparable regarding severe and mild or moderate pulmonary hypertension but definitely short term results are worse.

Objective: new management protocol to decrease the immediate postoperative differences between patients having Mitral stenosis with near systemic pulmonary hypertension compared to patients with mild or moderate pulmonary hypertension.

Patients and Methods: Prospective cohort single centre study was held in National heart institute, between 2016 and 2022, on patients undergoing Mitral valve surgery for Mitral stenosis with near …

Expression Levels Of Matrix Metalloproteinase 2 Protein In Preinvasive And Invasive Cervical Lesions And Its Clinicopathological Association: A North Indian Study, Rekha Sachan, Pushp L. Sachan, Ayushi Shukla, Malti Maurya, Munna L. Patel

Journal of Medicine in Scientific Research

Background Matrix metalloproteinase (MMPs) play an important role in development of female genital cancers and metastasis. The aim of this study was to analyze expression levels of MMP2 in various grades of cervical intraepithelial neoplasia (CIN) and squamous cell carcinoma (SCC) of cervix and its association with clinicopathological variables. Patients and methods This prospective study was carried out in a tertiary care center in Northern India over a period of 1 year. A total of 664 participants with gynecological complaints and unhealthy cervix were recruited for colposcopy. Of these, 70 biopsy samples were obtained from higher grade colposcopy lesions, where …