Medical Specialties Commons^™

Dimarco V. Lynch Homes-Chester County, Inc.: How Far Should West Virginia Go In Extension Of Physician Liability For Transmission Of Communicable Disease, Rebecca Coffield Moore

West Virginia Law Review

No abstract provided.

Cytolytic T Lymphocytes Specific For Tumors And Infected Cells From Mice With A Retrovirus-Induced Immunodeficiency Syndrome., Jennifer G. Erbe, Kathy A. Green, Karen M. Crassi, Herbert C. Morse, W R. Green

Dartmouth Scholarship

LP-BM5 retrovirus complex-infected C57BL/6 mice develop immunodeficiency, somewhat analogous to AIDS, termed murine AIDS (MAIDS). After secondary stimulation with syngeneic B-cell lymphomas from LP-BM5-infected mice, C57BL/6 mice produced vigorous CD8+ cytotoxic T lymphocytes specific for MAIDS-associated tumors. An anti-LP-BM5 specificity was suggested because spleen and lymph node cells from LP-BM5-infected mice served as target cells in competition assays, and cells from LP-BM5, but not ecotropic, virus-infected mice functioned as secondary in vitro stimulators to generate cytotoxic T lymphocytes to MAIDS tumors.

Aids And The Homeless Of Boston, James J. O'Connell, Joan Lebow

New England Journal of Public Policy

Homeless persons with AIDS and HIV infection face significant health hazards during the daily struggle for survival on the streets and in the crowded shelters of our cities. This article offers a historical perspective on the evolution of the AIDS epidemic within the homeless population of Boston and examines the demographics, risk behaviors, and survival statistics of that epidemic. The Boston Health Care for the Homeless Program is presented as a model of service delivery that offers quality health care to homeless persons with AIDS while addressing the special needs of those bound by the immediacy of the next meal …

Altered Expression Of Adenovirus 12 Dna-Binding Protein But Not Dna Polymerase During Abortive Infection Of Hamster Cells, Lynne A. Lucher, Benjawan Khuntirat, Jiansheng Zhao, Peter C. Angeletti

Nebraska Center for Virology: Faculty Publications

Replication of human adenovirus type 12 DNA is blocked in abortively infected baby hamster kidney cells. The activity and accumulation of adenovirus 12 DNA polymerase is equivalent in infected hamster and human cell extracts. However, the accumulation of adenovirus type 12 DNA-binding protein is approximately 120-fold lower in extracts from infected hamster cells when compared to infected permissive human cells. This difference in accumulation is not because of replication of viral DNA during productive infection, since this difference is observed in the presence of hydroxyurea. The DNA-binding protein from infected hamster cells retains the ability to bind denatured DNA-cellulose. An …

Fc Receptors For Igg (Fc Gamma Rs) On Human Monocytes And Macrophages Are Not Infectivity Receptors For Human Immunodeficiency Virus Type 1 (Hiv-1): Studies Using Bispecific Antibodies To Target Hiv-1 To Various Myeloid Cell Surface Molecules, Including The Fc Gamma R., R I. Connor, N B. Dinces, A L. Howell, J L. Romet-Lemonne, J L. Pasquali, M W. Fanger

Dartmouth Scholarship

Fc gamma Rs (Fc gamma RI, Fc gamma RII, and Fc gamma RIII) are highly expressed on human mononuclear phagocytes and function in the clearance of immune complexes and opsonized pathogens. We have examined the role of Fc gamma R in mediating antibody-dependent clearance of HIV-1 by human monocytes and monocyte-derived macrophages by using bispecific antibodies (BsAbs) to independently target the virus to Fc gamma RI, Fc gamma RII, or Fc gamma RIII. Virus production was markedly reduced in monocytes cultured with strain HIV-1IIIB opsonized with BsAbs that target the virus to either Fc gamma RI or Fc gamma RII …

Effect Of Delays In Processing On The Survival Of Mycobacterium Avium-M. Intracellulare In The Isolator Blood Culture System., C. Fordham Von Reyn, Stephen Hennigan, Sandra Niemczyk, Nicholas J. Jacobs

Dartmouth Scholarship

Concentrations of Mycobacterium avium-M. intracellulare ranging from 10(-1) to 10(3) CFU/ml were added to blood, placed in Isolator tubes, and held at room temperature for intervals ranging from 4 h to 56 days before being processed (centrifugation and culture on Middlebrook 7H10 agar). At all concentrations tested, M. avium-M. intracellulare was recovered after hold times ranging from 4 h to 7 days; the number of final CFU actually increased progressively for hold times of 8 h or more. Hold times of up to 7 days did not increase the time from processing to the first appearance of visible colonies. At …

Inhibition Of Growth Of Toxoplasma Gondii In Cultured Fibroblasts By Human Recombinant Gamma Interferon., E. R. Pfefferkorn, Paul M. Guyre

Dartmouth Scholarship

The growth of Toxoplasma gondii in cultured human fibroblasts was inhibited by recombinant human gamma interferon at concentrations of 8 to 16 U/ml. The interferon was titrated by observing a total inhibition of parasite plaque formation 7 days after infection. Inhibition of the growth of T. gondii in the early days after infection was measured by marked reductions in the incorporation of radioactive uracil, a precursor that can only be used by the parasites. This assay showed that when cells were pretreated with gamma interferon for 1 day and then infected, inhibition of T. gondii growth could be readily detected …

Immunological Approaches To The Diagnosis Of Systemic Candidiasis, Gene Elizabeth Burges

MUSC Theses and Dissertations

The increasing incidence of systemic candidiasis and the difficulties diagnosing the disease on the basis of clinical data and culture results have prompted investigation into immunological methods of diagnosis. Detection of anti-Candida antibodies has proved to be not sufficiently specific for diagnosis of systemic disease since antibodies are frequently found in the normal individual in whom the organism lives as a commensal and in superficially infected individuals. Our project resulted in the development of a new quantitative immunofluorescence assay for quantitation of anti-Candida antibodies which is able to differentiate quite well (p< 0.0005) between systemically infected patients and normals or superficially infected patients. We have also investigated the value of purified antigens and have shown that the use of cytoplasmic protein antigens rather than those containing cell wall polysaccharide confers greater specificity on a qualitative test for anti-Candida antibodies but that a carbohydrate-containing antigen is adequate for differentiating systemic disease when a quantitative test is used. Attempts to diagnose systemic candidiasis by detection of circulating antigen have met with varying degrees of success. We were unable to detect antigen by counter-immunoelectrophoresis, a quantitative immunofluorescence inhibition assay, or an ELISA inhibition assay in the sera of rabbits in whom a Candida endocarditis was induced or in the sera of systemically infected patients. One postulated reason for this failure is the presence of the antigen in the form of circulating immune complexes. We have, therefore, investigated the possibility of the existence of complexes containing Candida antigen and antibody in the sera of patients with systemic candidiasis. Using four screening tests, a statistically significant difference (p < 0.05) was found for immune complex levels in a group of patients and a group of normal individuals. Isolation and characterization of immune complex-containing fractions from five patients with candidiasis indicated the presence of anti-Candida antibodies and/or antigen in at least three of them. Therefore, these fungal immune complexes can be assumed to exist and may play a role in the difficulties in detecting circulating antigen and, furthermore, may be involved in the pathogenesis and progression of disseminated candidiasis.

The Protective Role Of Superoxide Dismutase Against The Beta Cell Toxicity Of Diabetogenic Drugs, Samuel Evans Gandy Iii

MUSC Theses and Dissertations

Superoxide dismutase (SOD) is a scavenger of the toxic superoxide radical and thereby protects the integrity of cell components from oxidative damage. This enzyme is an essential part of the biological defense system of aerobic cells, both directly by removing the superoxide anion and indirectly by preventing the formation of hydroxyl radical when superoxide reacts with hydrogen peroxide. The chemical diabetogenic agent streptozotocin, alloxan and Vacor are reported to directly inhibit the activity of this enzyme. The role of SOD in protection of pancreatic beta cells from the toxicity of diabetogenic drugs and the interaction of the drug with enzyme …

Sero-Epidemiology Of Q Fever With Special Reference To Complications In A Hospital Population Of Southern California, Ajit Singh Randhawa

Loma Linda University Electronic Theses, Dissertations & Projects

[Abstract Not Included]

Two Attempted Investigations Into The Complexities Of Staphylococcal Disease, Harriet M. Sands

Loma Linda University Electronic Theses, Dissertations & Projects

Two investigations were attempted to add insight into the multiple problems related to staphylococcal infections. A comparison study was made of the hospital expenses of a group of hospitalized patients whose apparently clean surgical wounds developed staphylococci, and a comparable group whose surgical wounds healed without infection.

A review of approximately one hundred medical records for the pilot study revealed three instances of staphylococcal infection in apparently clean surgical wounds. The three patients required 239 days of hospitalization at a total hospital cost of $13,702.11. The three comparable patients whose surgical wounds remained clean required 27 days hospitalization at a …

Identification Of Emotional Needs Of Patients With Infectious Hepatitis, Elizabeth Tidmore Shafqat

Loma Linda University Electronic Theses, Dissertations & Projects

It was the purpose of this study to identify the emotional needs of patients with infectious hepatitis through the use of nursing process studies. The information obtained, it was hoped, would be helpful in giving nurses better insight into the emotional needs of these patients and to give a basis for planning nursing care.

The descriptive survey was the method used; the tool, the nursing process study. Nursing care was given to six patients between the ages of 18 and 45 who had infectious hepatitis without other complications.

The nursing process studies were filled out by the investigator on each …

Evaluation Of The Photometric Assay Method To Quantitate The Bactericidal Action Of Normal And Immune Rabbit Sera On Salmonella Pullorum, Alice Margaret Rahn

Loma Linda University Electronic Theses, Dissertations & Projects

The bactericidal antibody test depends on the presence of sufficient complement, magnesium ions, and specific antibody to produce killing of certain gram negative bacilli. This study was undertaken to demonstrate whether or not Salmonella pullorum is an organism which is susceptible to this killing action.

Different amounts of test serum containing antibody, constant amounts of complement and magnesium saline diluent were placed together and held in a 37°C water bath for a one hour reaction time. During this interval, the antibody-complement system was given an opportunity to kill the susceptible bacteria. Then, a constant volume of nutrient broth was added …

Replicating Single-Cycle Adenovirus Vectors Generate Amplified Influenza Vaccine Responses, Catherine M. Crosby, William E. Matchett, Stephanie S. Anguiano-Zarate, Christopher A. Parks, Eric A. Weaver, Larry R. Pease, Richard J. Webby, Michael A. Barry

Nebraska Center for Virology: Faculty Publications

Head-to-head comparisons of conventional influenza vaccines with adenovirus (Ad) gene-based vaccines demonstrated that these viral vectors can mediate more potent protection against influenza virus infection in animal models. In most cases, Ad vaccines are engineered to be replication-defective (RD-Ad) vectors. In contrast, replication-competent Ad (RC-Ad) vaccines are markedly more potent but risk causing adenovirus diseases in vaccine recipients and health care workers. To harness antigen gene replication but avoid production of infectious virions, we developed “single-cycle” adenovirus (SC-Ad) vectors. Previous work demonstrated that SC-Ads amplify transgene expression 100-fold and produce markedly stronger and more persistent immune responses than RD-Ad vectors …