Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Medical Toxicology
Aflatoxin B1 Metabolism In Mammalian Pulmonary Tissue, J. Michael Eichelberger
Aflatoxin B1 Metabolism In Mammalian Pulmonary Tissue, J. Michael Eichelberger
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Aflatoxin B1 (AFB1) is a potent dietary hepatocarcinogen and may be a lung carcinogen when inhaled. To study the relative ability of lung and liver to metabolize AFB1, a susceptible (Swiss-Webster rat) and resistant species (Syrian golden hamster) were pretreated with inducing agents in order to identify specific AFB1 metabolizing enzymes in each tissue.
Analysis of AFB1-exo-epoxide (AFBO) formation, O-dealkynation assays, and protein immunoblots demonstrated that cytochrome P450 (CYP) 1A proteins were overexpressed in both the lung and liver of hamsters pretreated with 3-methylchyolanthrene (3-MC). Only CYP1A1 was expressed in the lung …