Medical Physiology Commons^™

The Effects Of Cigarette Smoking And Vitamin E On The Morphology Of Mice Testes, Kathleen Grams Hall

Masters Theses

Epidemiological studies have produced evidence to support an association between cigarette smoking and abnormal sperm morphology. At the least , an effect on sperm morphology indicates the involvement of the testis. Therefore, the research was designed to survey the potential of cigarette smoking for inducing histopathology in the testes of mice, and if there was damage, would vitamin E overcome it.

In the present study the testes of C57BL/6 mive exposed to cigarette smoke and/or vitamin E and 22 control mice were histologically processed. Sections stained with hematoxylin and eosin were assessed for total histopathology and revealed statistically significant differences …

Electrophysiology Of Flounder Intestinal Mucosa. I. Conductance Properties Ofthe Cellular And Paracellular Pathways, Dan R. Halm, Edward J. Krasny Jr., Raymond A. Frizzell

Neuroscience, Cell Biology & Physiology Faculty Publications

We evaluated the conductances for ion flow across the cellular and paracellular pathways of flounder intestine using microelectrode techniques and ion-replacement studies. Apical membrane conductance properties are dominated by the presence of Ba-sensitive K channels. An elevated mucosal solution K concentration, [K]_m, depolarized the apical membrane potential (ψ_a) and, at [K]_m less than 40 mM, the K dependence of ψ_a was abolished by 1-2 mM mucosal Ba. The basolateral membrane displayed Cl conductance behavior, as evidenced by depolarization of the basolateral membrane potential (ψ_b) with reduced serosal Cl concentrations, [Cl]_s. …

Electrophysiology Of Flounder Intestinal Mucosa. Ii. Relation Of The Electrical Potential Profile To Coupled Nacl Absorption, Dan R. Halm, Edward J. Krasny Jr., Raymond A. Frizzell

Neuroscience, Cell Biology & Physiology Faculty Publications

We characterized the hyperpolarization of the electrical potential profile of flounder intestinal cells that accompanies inhibition of NaCl cotransport. Several observations indicate that hyperpolarization of ψ_a and ψ_b (Δψ_a,b) results from inhibition of NaCl entry across the apical membrane: (a) the response was elicited by replacement of mucosal solution Cl or Na by nontransported ions, and (b) mucosal bumetanide or serosal cGMP, inhibitors of NaCl influx, elicited Δψ_a,b and decreased the transepithelial potential (ψ_t) in parallel. Regardless of initial values, ψ_a and ψ_b approached the equilibrium potential for K (E^{K …}