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Mri Relaxation Rates: A Quantitative Approach To Track Tumour Cells Expressing Maga, Anindita Sengupta
Mri Relaxation Rates: A Quantitative Approach To Track Tumour Cells Expressing Maga, Anindita Sengupta
Electronic Thesis and Dissertation Repository
Using magnetic resonance imaging, relaxation rate measurements were
performed in cancer cells overexpressing a magnetotactic bacterial gene, MagA.
Measurements of magnetic resonance relaxation rates in this expression
system is important for optimizing cell detection and specificity, for developing
quantification methods, and for refinement of gene-based iron contrast using
magnetosome associated genes. We measured the total transverse
relaxation rate (R2*), its irreversible and reversible components (R2 and R2′,
respectively) and the longitudinal relaxation rate (R1) in MDA-MB-435 tumor cells.
Clonal lines overexpressing MagA were cultured in the presence and absence of
iron supplementation, and mounted in a …