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Association Of Ace Polymorphism And Diabetic Nephropathy In South Indian Patients, Vijay Viswanathan, Yanqing Zhu, Karthik Bala, Stephen Dunn, Chamukuttan Snehalatha, Ambady Ramachandran, Muthu Jayaraman, Kumar Sharma
Association Of Ace Polymorphism And Diabetic Nephropathy In South Indian Patients, Vijay Viswanathan, Yanqing Zhu, Karthik Bala, Stephen Dunn, Chamukuttan Snehalatha, Ambady Ramachandran, Muthu Jayaraman, Kumar Sharma
Department of Medicine Faculty Papers
Objective: To study the association of ACE gene polymorphism and diabetic nephropathy in South Indian subjects.
Setting: Outpatient clinic of a specialized hospital.
Patients: The study included 109 South Indian type 2 diabetic patients (72 males and 37 females; age 56.7±9.0 years, mean±SD). The patients were subdivided into two groups: nephropathic (n=86) and normoalbuminuric patients (n=23).
Interventions: Genomic DNA was isolated from the peripheral blood leukocytes. To determine the ACE genotype, genomic DNA was amplified by PCR initially using a flanking primer pair and, subsequently when necessary, with a primer pair that recognizes the insertion specific sequence for confirmation of …