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Full-Text Articles in Genetic Processes
Role Of Evolutionarily Conserved G4 Structures In Enhancer-Promotor Interactions, Shahem Alqudah
Role Of Evolutionarily Conserved G4 Structures In Enhancer-Promotor Interactions, Shahem Alqudah
Honors Theses
Gene regulation is governed by complex networks of interactions between enhancers and promoters, regions of the genome that are typically distant from one another yet functionally linked through chromatin looping. Recent research has revealed the significant role of Gquadruplexes (G4s) in facilitating these interactions. This thesis explores the role of long Gquadruplex regions (LG4s) in enhancer–promoter communication, proposing that these regions mediate physical interactions between enhancers and their target promoters through direct G4:G4 interactions. Using a chromatin conformation capture (EQuIP-seq), electrophoretic mobility shift assays (EMSA), and luciferase reporter assays, the study demonstrates that LG4s may drive gene expression by promoting …
A Comparative Analysis Of Nf-Κb1 Gene Regulatory Sequence Methylation In Normotensive And Hypertensive Kenyans, Aaryan Barlas Piracha
A Comparative Analysis Of Nf-Κb1 Gene Regulatory Sequence Methylation In Normotensive And Hypertensive Kenyans, Aaryan Barlas Piracha
Honors Theses
Accounting for the majority of deaths worldwide, non-communicable diseases (NCDs) present the greatest health challenge of the twenty-first century. Specifically, cardiovascular diseases (CVDs) exceed all other NCDs in annual deaths and especially affect low- and middle-income countries (LMICs). Hypertension, being the primary risk factor for CVD, affects over 75% of adults in LMICs due to inadequate health care and preventative measures. Additionally, epigenetic modifications of DNA are important mechanisms that regulate gene expression; DNA methylation, in particular, affects cytosine residues in cytosine-phosphate-guanine (CpG) islands on regulatory sequences. Previous research in our laboratory analyzed percent methylation at 8 different CpG islands …
The Use Of Embark In Teaching About Genetic Relatedness, Anna Bonvillain, Tori Trammel
The Use Of Embark In Teaching About Genetic Relatedness, Anna Bonvillain, Tori Trammel
Honors Theses
Because of the increasing importance of precision medicine, it is vital that future healthcare providers master concepts related to genetic variation taught during their undergraduate classes. However, studies have shown that physicians often lack an adequate understanding of genetics, which serves as a hindrance to effectively caring for their patients. To address this issue, we created a collaborative active-learning protocol to improve pre-health students' comprehension of key concepts such as genetic relatedness and the source of genetic variation between siblings. Our worksheet guides students to compare the genetic profiles of two canine siblings using the Embark DNA genotyping platform. Embark …