Medical Sciences Commons^™

Development Of A Mathematical Model Of Renal Function For Clinical Application, Barbara A. Holshouser

Loma Linda University Electronic Theses, Dissertations & Projects

This model describes the orthoiodohippurate (OIH) distribution and clearance in the renovascular system. The model is described by five compartments: the blood, left and right kidneys, bladder and the red blood cell compartment. Data for these compartments, except the RBC compartment, are collected with scintillation detectors monitoring OIH tagged with 1-131 as the radiopharmaceutical passes through each compartment. Time/ activity curves are plotted for the data and used as inputs to the model parameter estimation routine. The compartments are described by a set of first order ordinary differential equations solved using the Adam's methods. For parameter estimation, an iterative predictor-corrector …

Aspects Of Anuran Metabolism: Effects Of Chronic Hypoxia On Maximal Oxygen Uptake Rates And The Fate Of Lactic Acid, Thomas Charles Solberg

Dissertations and Theses

Some aspects of anuran metabolism are examined, with special emphasis on possible limitations to aerobic metabolism and the effect of chronic hypoxia acclimation on maximal rates of aerobic metabolism and the metabolic fate of lactic acid accumulated after anaerobic metabolism.

Effects Of The Serotonin Depletor Parachlorophenylalanine Upon Shock-Induced Aggression And Pressing Responses In Rats, Robert G. Sewell

Masters Theses

The literature concerning the effects of d, l-parachlorophenylalanine (PCPA) upon shock-induced aggression (SIA) is reviewed and found to be inconsistent. PCPA, a known serotonin depletor, has behavioral effects in a variety of other procedures which collectively suggest that PCPA should produce SIA enhancement. The present study was designed to analyze PCPA (300 mg/kg) effects upon SIA in rats restrained spatially close to an inanimate target and panel operandum. The results showed marked increases in both aggressive biting and panel pressing for several days following each PCPA treatment for each subject tested. These data were interpreted to indicate that serotonin depletion …

Muscle Catabolism During A Protein Sparing Modified Fast As Measured By Urinary 3-Methylhistidine, Judith D. Nielsen

Loma Linda University Electronic Theses, Dissertations & Projects

The urinary excretion of 3-Methylhistidine was used as an index of muscle catabolism in four groups of obese persons receiving a Protein Sparing Modified Fast (PSMF). The composition of the supplements were as follows: Group I: 75 g protein, 25 g complex carbohydrate (starch) per day; Group II: 25 g protein, 75 g complex carbohydrate (starch) per day; Group III: 75 g protein, 25 g simple carbohydrate (glucose) per day; Group IV: 25 g protein, 75 g simple carbohydrate (glucose) per day. The PSMF lasted for seven days and a 24-hour urine sample was collected at the beginning of the …

The Role Of The Parotid Salivary Glands In The Regulation Of Insulin Secretion, Kenneth L. Millard

Loma Linda University Electronic Theses, Dissertations & Projects

Stress of handling, partial or complete sialoadenectomy, and the administration of parotid hormone (PH), sodium fluoride (NaF), or guanidino-succinic acid (GSA) were examined for effects on fasting plasma glucose and insulin or effects on intravenous glucose tolerance in male Sprague-Dawley rats. Basal glucose and insulin were substantially elevated as a result of the stress of translocation in large animals, but were not altered in small animals. A rise in plasma insulin occured in control animals 20 minutes following anesthesia and the removal of a blood sample by cardiac puncture but not in animals that had been handled twice daily for …

Effects Of Withdrawing Diphenylhydantoin (Dilantin) On Matching To Sample Performance In Mentally Retarded Persons, Vicky June Davis

Masters Theses

The present study was designed to examine the effects of gradual DPH withdrawal on the matching to sample performance of three mentally retarded persons. The percentage of correct responses per session served as the dependent variable and the sample and comparison stimuli were red, green, and blue illumination of translucent response windows. The results showed that doses of diphenylhydantoin considerably lower than the suggested optimim therapeutic level can impair the performance of mentally retarded individuals on a simple discrimination task. As doses were reduced for each subject, there were increases in the percentage of correct responding with the highest percentage …

The Coronary Distribution Zone : Mathematical Modelling Of The Left Ventricular Epicardial Surface For Area Calculation, Three-Dimensional Rotaton And Display, S. Matts E. Sjölander

Loma Linda University Electronic Theses, Dissertations & Projects

A computer model of the left ventricular (LV) epicardial surface was developed from Jang’s truncated ellipsoidal model, which has a minor to major axis ratio of 6:10. The model was applied in calculating the area of a segment of the myocardium, jeopardized by an impending occlusion of its associated artery. The endangered segment of the LV muscle was also visualized in different projections by rotations in three space and graphical displays simulating the three dimensions.

The area formula for the surface of the ideal model , a truncated prolate spheroid, was derived and used in producing maps for manual calculations …

Distribution Of Maternal And Fetal Blood Flow Within Cotyledons Of The Sheep Placenta, Gordon G. Power, P. Steven Dale, Paul S. Nelson

Loma Linda University Electronic Theses, Dissertations & Projects

These studies explore the distribution of blood flow among small samples of placental tissue. Labeled microspheres (14 ± 1 pm) were Injected Into the left ventricle of six un-anesthetized ewes and Into the jugular vein of the fetus In utero. A total of 3,576 samples weighing 31. +11. (S.D.) mg were taken from seventeen cotyledons and counted for flow labels. Maternal and fetal flow within the cotyledons were both normally distributed with standard deviations of 44%. The maternal/ fetal flow ratio was less than 0.5 In 9% of the cotyledon, between 0.5 and 1.5 In 70%, and greater than 1.5 …

Lipoprotein Lipase Inhibitor Removal During Ultrafiltration Of Renal Failure Patients, Alisa Minear

Loma Linda University Electronic Theses, Dissertations & Projects

Plasma samples were obtained from seven hypertriglyceridemic (>150 mg/dl) chronic renal failure patients before and after regular hemodialysis. The effect of the plasma on the lipolytic activity of bovine milk lipoprotein lipase was investigated. The lipoprotein lipase activity, as measured by release of C¹⁴-labeled free fatty acid from triglyceride, was decreased 8-67% when incubated with uremic plasma as compared to the control plasma (44 mg/dl triglyceride). The inhibition noted prior to hemodialysis did not appear to be alleviated by the dialysis but subsequent treatment with an isolated ultrafiltration technique did appear to remove an inhibitory substance as …

Toxicity Of Root Canal Filling Materials On Hela Cells And Fibroblasts, Eldon P. Carman

Loma Linda University Electronic Theses, Dissertations & Projects

A study was undertaken to determine the concentration at which a sealer reaches a point of nontoxicity. An in vitro model utilizing HeLa cells and human fibroblasts was used to observe the effect of various concentrations of eight freshly mixed and eight set root canal sealers. Evaluation of toxicity was determined by cell survival which was measured by incorporation of ³H-thymidine into the nucleic acid components of each cell type used. Each dilution tested was allowed direct contact with the cells for 24 hours before toxicity evaluation. The results indicate that all sealers at concentrations presently used are cytotoxic …

The Rate Of The Healing Process In Venous Thrombi Produced By A Sclerosing Agent With Special Attention To Endothelial Lining, Kiyoshi Tanaka

Loma Linda University Electronic Theses, Dissertations & Projects

Despite extensive clinical and experimental investigations of venous thrombosis, relatively little is known about the early phases of healing of the venous thrombus. Soon after its discovery heparin has become the most popular agent for the prevention and treatment of venous thrombosis, but there are a number of unanswered questions regarding the most satisfactory treatment for venous thrombosis.

One area of controversy has been the duration of anticoagulation therapy for acute venous thrombosis. If the anticoagulant is discontinued too soon, the thrombus may continue to propagate. Prolonged anticoagulant therapy exposes the patient to the risk of serious hemorrhage. The objective …

Characteristics Of The Recovery Of The Coenzyme A-Synthesizing Protein Complex From Saccharomyces Cerevisiae, Stanley Joseph Tarnowski Jr.

Theses and Dissertations (ETD)

A multienzyme complex contained in Bakers' yeast (Saccharomyces cerevisiae) which synthesizes CoA has been named the coenzyme A-synthesizing protein complex (CoA-SPC). The CoA-SPC has been shown to be insoluble in the crude Bakers' yeast cell lysate formed by exposing the yeast cell to ether and dry ice. Only after solubilization has this multienzyme complex been shown to catalyze the formation of bound dephospho-CoA utilizing the substrates adenosine triphosphate, D-pantothenic acid and L-cysteine. A low molecular weight component or components of the soluble fraction of the yeast cell and chloride ion appears to be responsible for the solubilization of CoA-SPC. This …

Patterns Of Fetal Lamb Regional Cerebral Blood Flow During And After Prolonged Hypoxia, Stephen Ashwal, John S. Majcher, Nestor Vain, Lawrence D. Longo

Loma Linda University Electronic Theses, Dissertations & Projects

[Abstract not included]

Speculation: In the fetal lamb during prolonged intrauterine hypoxia, total and regional cerebral blood flows increase to the same extent without evidence of preferential shunting to critical brainstem or subcortical areas. Neuropathologic studies have indicated relative sparing of these areas during similar animal experimental or human neonatal conditions. This suggests that the pattern of hypoxic ischemic insult to the neonatal central nervous system associated with asphyxia may differ from that produced by hypoxia alone. In addition, during asphyxia these pathologic changes may result primarily from hypotension and decreased regional cerebral blood flow, or from regional metabolic derangements …

Cell Mediated Immunity By Cytotoxicity Assay And The Effect Of Corynebacterium Parvum And Radiation On Mice Bearing Herpes-Induced Tumors, Robert B. Stagg

Loma Linda University Electronic Theses, Dissertations & Projects

Cell mediated immune (CMI) cytotoxic reactivity of Balb/c mice against H238 cells, a Herpes simplex virus (HSV) Type 2 - induced sarcoma, was measured by the ¹²⁵IUdR release assay. The Balb/c mouse response to the growing HSV-induced tumor, treated with radiation and , was measured by survival rate, tumor growth and immune cytotoxicity of spleen and peritoneal exudate cells (PEC) as determined by ¹²⁵IUdR release assay. Subcutaneous (s.c.) inoculation of 1 x 10⁶ H238 cells (high dose) produced progressive tumor growth while s.c. inoculation of 1 x 10⁴ H238 cells (low dose) produced no tumors. A …

The Morphology Of The Canine Renal Lymphatic System, Kurt H. Albertine

Master's Theses

No abstract provided.

Computer Modeling Of Cardiac Electrical Activity By Polarcardiogram Patterns, Daniel K. Berry

Loma Linda University Electronic Theses, Dissertations & Projects

The objective of this thesis is to provide for the cardiologist polarcardiograms and additional diagnostic criteria which will enable him to diagnose heart disease more easily. Other contributions are those of: (a) providing a system of data acquisition and (b) analyzing Dr. Gordon Dower’s criteria for myocardial infarction using the programs provided in this thesis.

The problem inherent in the reading of a polarcardiogram is the identification of the "loop" patterns with the associated heart disease. This can be accomplished by reading and interpreting both vectorcardiograms and electrocardiograms as well as angiograms, then relating the interpretation to the polarcardiogram patterns. …

Localization Of Specific Mrnas For Human Placental Lactogen And Human Chorionic Gonadotropin-Alpha And Beta Subunits, Richard Fehn

Theses Digitization Project

No abstract provided.

Outcome Evaluation Of Medical Care Utilizing Goal Attainment Scaling, Bonnie G. Little

Dissertations and Theses

The purpose for this study was to develop guidelines for the implementation of Goal Attainment Sealing (a mental health measurement technique) in the medical care setting. As a graduate student of social work, I have become involved in the delivery of medical care and am interested in the dynamics of psycho-social variables as they affect the structure, delivery, and outcome of medical services.

Role Of Cytochrome P-450 In Methemoglobin Formation By Primaquine With Hamster Liver Microsomes, Tommy L. Hicks

Loma Linda University Electronic Theses, Dissertations & Projects

The role of cytochrome P-450 in in vitro methemoglobin formation by primaquine was investigated through a series of experiments with golden-Syrian hamsters, treated before sacrificing with phenobarbital or 20-methylcholanthrene to increase the cytochrome P-450 content or with cobaltous chloride to cause its decrease. To determine the extent of induction in the liver microsomal cytochrome P-450-dependent mixed-function oxidases, cytochrome P-450 content and formaldehyde from the 0-demethylation of p-nitroanisole were measured. A 90% increase occurred in cytochrome P-450 content with phenobarbital pretreatment and an increase of 102% was observed in p-nitroanisole 0-demethylation. Formaldehyde from primaquine increased 64%. The methylcholanthrene treatment produced a …

Restriction Endonuclease Analysis Of Bacteriophage P1 Dna And Its Derivative Hybrid Dnas, Gregory Alan Schulz

Master's Theses

No abstract provided.

An Investigation Of The Activity Of Specific Antibody Isolated Using Immunoadsorbents, Guy Joseph Agostino

Master's Theses

No abstract provided.

Computer Assisted Electron Spin Resonance Spectral Analysis Of Trapped Radicals, Philip Mook Wah Law

Loma Linda University Electronic Theses, Dissertations & Projects

A set of general purpose FORTRAN IV programs have been written during this study to assist in the analysis of complex spectra. An interactive approach is adopted, utilizing the computer and graphics terminals of the Scientific Computation Facility at Loma Linda University. Some procedures carried out are inversion, coordinates scaling, smoothing, differentiation and integration, correction for slanting baseline, addition and subtraction, and spectral simulation.

As an application, the programs are used to assist in the study of free radicals in gamma-irradiated single crystals of 1,2,3,- benzene tricarboxylic acid dihydrate by electron spin resonance spectroscopy. Is is found that the predominant …

A Batch Method For The Isolation Of Immunogenically Pure Igg, Iga, And Igm From Human Sera, Samuel Christopher Powell

Chemistry & Biochemistry Theses & Dissertations

A method is described for preparing an enriched fraction of the three major antibodies, immunoglobulins IgG, IgA and IgM, from normal human serum by a batch method. Selective precipitation of these proteins was carried out in successive steps using polyethylene glycol (PEG) of average molecular weight 6,000 at concentrations of 7%, 12% and 14% (16) . Zinc sulfate (10 mM) was used to remove highly immunogenic alpha₂ macroglobulin from the IgM fraction. IgA, IgM and IgG were further purified by adsorption methods using DEAE-cellulose. Quantitation and qualitation of the enriched fractions showed nearly complete separation of each of the …

The Histochemical Distribution Of Placental Calcium And Alkaline Phosphatase Activity Following Fetoplacental Dissociation In The Albino Rat, Eric Sigmond

Master's Theses

No abstract provided.

Ultrastructure And Chemical Composition Of Microconidial Walls Of Trichophyton Mentagrophytes, Christine Da-Ruh Wu

Dissertations

No abstract provided.

Similarities In Analgesia Produced By Cervical Probing And Intracranial Stimulation To The Mesencephalic Grey Matter, Kathleen Casey Westlake

Dissertations, Theses, and Masters Projects

No abstract provided.

An Analysis Of Blood Cortisol Levels In Oral Surgery Patients Under Various Levels Of Intravenous Medication, Fred L. Bunch

Loma Linda University Electronic Theses, Dissertations & Projects

This study was designed to objectively evaluate each pharmacological component of the Loma Linda intravenous sedation technique. The results were evaluated as a function of stress as measured by plasma cortisol levels.

The study was conducted on fifty patients in groups of ten. Blood samples were taken at set procedure intervals during dental extraction of third molar teeth. A component of the Loma Linda technique was deleted from each of the groups to ascertain its role in sedation and relief of stress.

Results show a trend toward reduction of plasma cortisol levels as the procedure progresses, indicating a measure of …

The Effect Of A Pufa Diet On Human Red Blood Cell Levels Of 2, 3-Dpg, Sandra A. Dassenko

Loma Linda University Electronic Theses, Dissertations & Projects

Fasting blood was collected at weekly intervals and the level of 2,3-DPG of the red blood cell was determined in 15 male subjects age 28 to 57 with serum cholesterol levels greater than 250 mg%. A lactovegetarian diet containing no cholesterol, a high level of dietary fiber and a P/S ratio of 4.0 was provided for 28 days.

During the course of the experimental period the 2,3-DPG increased by an average of 0.83> «moles/ml red blood cell which was highly significant (p^.001). This significant increase of 2,3-DPG which relates to an increased oxygen delivery capability of the red blood cells …

A Descriptive Study Of Women's Attitudes Towards Obtaining Or Not Obtaining Papanicolaou Smears, Linda J. Shults

Loma Linda University Electronic Theses, Dissertations & Projects

It was the purpose of this study to identify the attitudes of a selected group of women which influenced their participation in Papanicolaou testing. These attitudes were inferred from the reasons women gave for obtaining the test. The subjects were randomly selected from the telephone book of a medium-sized town in Southern California. The 135 women who participated in the telephone interview were considered to be the respondents in the study.

A review of the literature revealed that through the annual use of the Papanicolaou smear, approximately 11,000 deaths every year from uterine and cervical cancer could be prevented, Attitudes …

An Evaluation Of The Cytotoxicity Of Root Canal Filling Materials On Tissue Culture Cells In Vitro: Grossman's Sealer, N2 Permanent, Rickert's Sealer, And Cavit, Donald D. Antrim

Loma Linda University Electronic Theses, Dissertations & Projects

An in vitro study using radioactively-labeled tissue culture cells was conducted over a seven-month period on Grossman's sealer, Rickert's sealer, N₂ Permanent, and Cavit to determine the lasting and relative tissue toxicity of these materials.

From this study it was observed that all materials possess lasting tissue toxicity. When relative toxicity is considered, Grossman's is the most toxic, followed by N₂, Rickert's and Cavit in descending order of toxicity. Both Grossman's and N₂ would be considered highly toxic, with Rickert's displaying moderate toxicity. Cavit would be considered mild to moderate in toxicity.

The use of radioactively-labeled …