Diseases Commons^™

A Multicenter Analysis Of Nucleic Acid Quantification Using Aqueous Humor Liquid Biopsy In Retinoblastoma: Implications For Clinical Testing, Deborah H Im, Sarah Pike, Mark W Reid, Chen-Ching Peng, Shreya Sirivolu, Hans E Grossniklaus, G Baker Hubbard, Alison H Skalet, Kellyn N Bellsmith, Carol L. Shields, Sara E Lally, Andrew W Stacey, Bibiana J Reiser, Aaron Nagiel, Rachana Shah, Liya Xu, Jesse L Berry

Wills Eye Hospital Papers

PURPOSE: Retinoblastoma (RB) is most often diagnosed with clinical features and not diagnosed with tumor biopsy. This study describes tumor-derived analyte concentrations from aqueous humor (AH) liquid biopsy and its use in clinical assays.

DESIGN: Case series study.

PARTICIPANTS: Sixty-two RB eyes from 55 children and 14 control eyes from 12 children from 4 medical centers.

METHODS: This study included 128 RB AH samples including: diagnostic (DX) samples, samples from eyes undergoing treatment (TX), samples after completing treatment (END), and during bevacizumab injection for radiation therapy after completing RB treatment (BEV). Fourteen-control AH were analyzed for unprocessed analytes (double-stranded DNA …

The Tumor Microenvironment Regulates Retinoblastoma Cell Survival, Zachary K. Goldsmith

Theses and Dissertations (ETD)

Retinoblastoma (Rb) is the most common intraocular malignancy in children comprising 4% of all pediatric tumors. Early intervention increases survival rates up to 95% in developed countries; being survival rates associated to socio-economic status. Despite the high survival rates in developed countries, preservation of the eye and vision are continuing challenges in the management of Rb. Vitreous seeds constitute the greatest challenge in treatment of Rb. The unique location of these seeds within the vitreous makes them difficult to treat. Viable seeds showed reduced proliferative capacity and metabolism. making the majority refractory to current chemotherapeutics. This prompted the development of …

Retinoblastoma Tells The Story Of Our Health Care System, Irshad Soomro, Muhammad Nadeem Khan, Suhail Muzaffar, Naila Kayani, Shahid Pervez, Akbar Shah Hussainy, Rashida Ahmed, Sheema H. Hasan

Rashida Ahmed

Objective: To review cases of retinoblastoma. Setting: Department of Pathology Aga Khan University Hospital Karachi. Method: Twenty three specimens from cases of retinoblastoma received over a period of eight years were routinely processes and stained with haematoxylin and Eosin stain Other stains were used for tuberculosis and melanin. Immunochemistry was resorted to in undifferentiated tumors. Results: Over 60% cases of retinoblastoma were diagnosed after 5 years and nine cases showed involvement of opti-nerve. Conclusion: Late diagnosis of retinoblastoma effects the stage of the tumors and the prognisis.

Ajulemic Acid Induces Cell Death In Retinoblastoma Cells, Emily A. Evans

Honors Theses

Retinoblastoma is a cancer of the retina affecting one in every 15,000-20,000 births. It is normally diagnosed in infancy or early childhood and although it is treatable, enucleation is commonly required. Other treatments include radiation, photocoagulation, or cryotherapy and are often followed by chemotherapy. In an attempt to find an alternate method of treatment that lowers the frequency of enucleation and the serious side-effects resulting from current treatments, ajulemic acid (AJA), a synthetic derivative of Cannabis, has been used to treat retinoblastoma cells. Thus far, significant cell death has occurred among retinoblastoma cells that have been treated with AJA concentrations …

La Tomografía Computarizada Y La Resonancia Magnética En Patologías Frecuentes De Órbita Y Vía Óptica. Una Revisión, Lina Marcela Acuña Bedoya, Sara Viviana Angulo Sánchez

Ciencia y Tecnología para la Salud Visual y Ocular

Objetivo: determinar las aplicaciones clínicas de la tomografía axial computarizada (TAC) y la resonancia magnética (RM) en el diagnóstico y manejo de algunas patologías frecuentes de órbita y vía óptica. Materiales y métodos: se realizó una revisión bibliométrica empleando estrategias de búsqueda en las bases de datos Pubmed y Medline, entre otras. Resultados y discusión: con la presente revisión se identificó el examen de preferencia por ciertos autores entre el 2009 y el 2012 para patologías frecuentes de órbita y vía óptica. Sin embargo, en el proceso de búsqueda se evidenció que algunas presentaron frecuencias similares durante un periodo determinado, …

Retinoblastoma Tells The Story Of Our Health Care System, Irshad Soomro, Muhammad Nadeem Khan, Suhail Muzaffar, Naila Kayani, Shahid Pervez, Akbar Shah Hussainy, Rashida Ahmed, Sheema H. Hasan

Department of Pathology and Laboratory Medicine

Objective:

To review cases of retinoblastoma.

Setting:

Department of Pathology Aga Khan University Hospital Karachi.

Method:

Twenty three specimens from cases of retinoblastoma received over a period of eight years were routinely processes and stained with haematoxylin and Eosin stain Other stains were used for tuberculosis and melanin. Immunochemistry was resorted to in undifferentiated tumors.

Results:

Over 60% cases of retinoblastoma were diagnosed after 5 years and nine cases showed involvement of opti-nerve.

Conclusion:

Late diagnosis of retinoblastoma effects the stage of the tumors and the prognisis.