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- DHA (2)
- RPE (2)
- AA (1)
- AMD (1)
- Cellular senescence (1)
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- Cones (1)
- Elovanoids (1)
- IQ motif containing GTPase activating protein (IQGAP1) (1)
- Invasive OSSN (1)
- MALDI molecular imaging (1)
- Macula (1)
- Macular degeneration (1)
- Mammalian target of rapamycin (mTOR) (1)
- Neuroprotection (1)
- Ocular surface squamous neoplasia (OSSN) (1)
- Periphery (1)
- Peroxisome (1)
- Preinvasive OSSN (1)
- Retinal pigment epithelium (1)
- Rods (1)
- VLC-PUFA (1)
- VLC-PUFAs (1)
- Yes-associated protein-1 (YAP-1) (1)
- Zambia (1)
Articles 1 - 6 of 6
Full-Text Articles in Diseases
Dha Shortage Causes The Early Degeneration Of Photoreceptors And Rpe In Mice With Peroxisomal Β-Oxidation Deficiency, Daniëlle Swinkels, Sai Kocherlakota, Yannick Das, Adriaan D. Dane, Eric J.M. Wever, Frédéric M. Vaz, Nicolas G. Bazan, Paul P. Van Veldhoven, Myriam Baes
Dha Shortage Causes The Early Degeneration Of Photoreceptors And Rpe In Mice With Peroxisomal Β-Oxidation Deficiency, Daniëlle Swinkels, Sai Kocherlakota, Yannick Das, Adriaan D. Dane, Eric J.M. Wever, Frédéric M. Vaz, Nicolas G. Bazan, Paul P. Van Veldhoven, Myriam Baes
School of Medicine Faculty Publications
PURPOSE. Patients deficient in peroxisomal β-oxidation, which is essential for the synthesis of docosahexaenoic acid (DHA, C22:6n-3) and breakdown of very-long-chain polyunsaturated fatty acids (VLC-PUFAs), both important components of photoreceptor outer segments, develop retinopathy present with retinopathy. The representative mouse model lacking the central enzyme of this pathway, multifunctional protein 2 (Mfp2−/−), also show early-onset retinal decay and cell-autonomous retinal pigment epithelium (RPE) degeneration, accompanied by reduced plasma and retinal DHA levels. In this study, we investigated whether DHA supplementation can rescue the retinal degeneration of Mfp2−/− mice. METHODS. Mfp2+/− breeding pairs and their offspring were fed a 0.12% DHA …
Rod-Specific Downregulation Of Omega-3 Very-Long-Chain Polyunsaturated Fatty Acid Pathway In Age-Related Macular Degeneration, William C. Gordon, Marie Audrey I. Kautzmann, Bokkyoo Jun, Megan L. Cothern, Zhide Fang, Nicolas G. Bazan
Rod-Specific Downregulation Of Omega-3 Very-Long-Chain Polyunsaturated Fatty Acid Pathway In Age-Related Macular Degeneration, William C. Gordon, Marie Audrey I. Kautzmann, Bokkyoo Jun, Megan L. Cothern, Zhide Fang, Nicolas G. Bazan
School of Medicine Faculty Publications
Docosahexaenoic acid (DHA; 22:6) plays a key role in vision and is the precursor for very-long-chain polyunsaturated fatty acids (VLC-PUFAs). The release of 32- and 34-carbon VLC-PUFAs and DHA from sn-1 and sn-2 of phosphatidylcholine (PC) leads to the synthesis of cell-survival mediators, the elovanoids (ELVs) and neuroprotectin D1 (NPD1), respectively. Macula and periphery from age-related macular degeneration (AMD) donor retinas were assessed for the availability of DHA-related lipids by LC-MS/MS-based lipidomic analysis and MALDI-molecular imaging. We found reduced retina DHA and VLC-PUFA pathways to synthesize omega-3 ELVs from precursors that likely resulted in altered disks and photoreceptor loss. Additionally, …
Yes-Associated Protein-1 Overexpression In Ocular Surface Squamous Neoplasia; A Potential Diagnostic Marker And Therapeutic Target, Peter Julius, Stepfanie N. Siyumbwa, Fred Maate, Phyllis Moonga, Guobin Kang, Trevor Kaile, John T. West, Charles Wood, Peter C. Angeletti
Yes-Associated Protein-1 Overexpression In Ocular Surface Squamous Neoplasia; A Potential Diagnostic Marker And Therapeutic Target, Peter Julius, Stepfanie N. Siyumbwa, Fred Maate, Phyllis Moonga, Guobin Kang, Trevor Kaile, John T. West, Charles Wood, Peter C. Angeletti
School of Graduate Studies Faculty Publications
Yes-associated protein-1 (YAP-1) is a Hippo system transcription factor, which serves as an oncogene in squamous cell carcinoma, and several solid tumors when the Hippo pathway is dysregulated. Yet, the activity of YAP-1 in ocular surface squamous neoplasia (OSSN) has not been determined. Here, we investigate the relationship between YAP-1 overexpression and OSSN. Using a cross-sectional study design, we recruited 227 OSSN patients from the University Teaching Hospitals in Lusaka, Zambia. Immunohistochemistry was used to assess YAP-1 protein overexpression in tumor tissue relative to surrounding benign squamous epithelium. OSSN patient samples (preinvasive, n = 62, 27% and invasive, n = …
7-Ketocholesterol Promotes Retinal Pigment Epithelium Senescence And Fibrosis Of Choroidal Neovascularization Via Iqgap1 Phosphorylation-Dependent Signaling, Haibo Wang, Aniket Ramshekar, Thaonhi Cung, Chris Wallace-Carrete, Chandler Zaugg, Jasmine Nguyen, Gregory J. Stoddard, M. Elizabeth Hartnett
7-Ketocholesterol Promotes Retinal Pigment Epithelium Senescence And Fibrosis Of Choroidal Neovascularization Via Iqgap1 Phosphorylation-Dependent Signaling, Haibo Wang, Aniket Ramshekar, Thaonhi Cung, Chris Wallace-Carrete, Chandler Zaugg, Jasmine Nguyen, Gregory J. Stoddard, M. Elizabeth Hartnett
School of Medicine Faculty Publications
Accumulation of 7-ketocholesterol (7KC) occurs in age-related macular degeneration (AMD) and was found previously to promote fibrosis, an untreatable cause of vision loss, partly through induction of endothelial-mesenchymal transition. To address the hypothesis that 7KC causes mesenchymal transition of retinal pigment epithelial cells (RPE), we exposed human primary RPE (hRPE) to 7KC or a control. 7KC-treated hRPE did not manifest increased mesenchymal markers, but instead maintained RPE-specific proteins and exhibited signs of senescence with increased serine phosphorylation of histone H3, serine/threonine phosphorylation of mammalian target of rapamycin (p-mTOR), p16 and p21, β-galactosidase labeling, and reduced LaminB1, suggesting senescence. The cells …
Incidence Of Total Blindness After Central Retinal Artery Occlusion With Ocular Neovascularization, Harshvardhan Chawla, Hayley J. Redrick, Joshua T. Pannell, D. Anthony Mazzulla, Joseph D. Benevento, Sidharth Puri
Incidence Of Total Blindness After Central Retinal Artery Occlusion With Ocular Neovascularization, Harshvardhan Chawla, Hayley J. Redrick, Joshua T. Pannell, D. Anthony Mazzulla, Joseph D. Benevento, Sidharth Puri
School of Medicine Faculty Publications
Annual Meeting Association for Research in Vision and Ophthalmology, ARVO 2023, April 24 - 27, 2023, New Orleans, LA
A Modified Bicanalicular Crawford Placement Method For Congenital Nasolacrimal Duct Obstruction: Reducing Need For Operative Room Removal, Ellen E. Ingram, John P. Thompson, Elizabeth M. Bozner, Armin Avdic, Charles Reeder, George S. Ellis, Austin M. Pharo
A Modified Bicanalicular Crawford Placement Method For Congenital Nasolacrimal Duct Obstruction: Reducing Need For Operative Room Removal, Ellen E. Ingram, John P. Thompson, Elizabeth M. Bozner, Armin Avdic, Charles Reeder, George S. Ellis, Austin M. Pharo
School of Medicine Faculty Publications
Annual Meeting Association for Research in Vision and Ophthalmology, ARVO 2023, April 24 - 27, 2023, New Orleans, LA