Dietetics and Clinical Nutrition Commons^™

Attenuating The Side Effects Of Caloric Restriction Through Exercise And Increased Protein Intake, Jay A. Petersen

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

The effects of caloric restriction (CR) on weight loss and health outcomes are documented, but few controlled studies have addressed its effect on performance. Fat free mass (FFM) is reduced during CR, which may impair performance. The purpose of this thesis was to explore the capacity of these strategies to attenuate the side-effects of calorie-restricted weight loss: Exercise, which preserves FFM during CR, may be employed to maintain performance in an energy-deficient state, and a high protein intake may work in combination with exercise to further protect FFM and performance. Two studies were utilized to address this purpose. In study …

The Relationships Between The Home Food Environment And Weight Status Among Children And Adolescents, Ages 6-17 Years, Martha J. Nepper

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

The home food environment is an important setting in regard to a child’s dietary intake and the development of obesity, since 65% to 72% of daily calories are consumed in the home. Research is beginning to explore how the home food environment may influence children’s weight status. It is suggested that homes with healthy weight children are more likely to have healthier food options available and limit access to unhealthy foods. Prior research on the influence of the home food environment on children and adolescent’s weight status is not clear, as some researchers have found that the home food environment …

Transport Of Bovine Milk Exosomes By Human Colon Carcinoma Caco-2 Cells And Rat Small Intestinal Iec-6 Cells, Tovah Wolf

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

We have reported that microRNAs (miRNAs, miRs) in bovine milk regulate human genes. In milk, many miRNAs are encapsulated in exosomes, thereby conferring protection against degradation and a pathway for intestinal transport of miRNAs. We hypothesized that the uptake of bovine exosomes in human intestinal colon carcinoma Caco-2 cells and rat primary small intestinal IEC-6 cells is mediated by endocytosis. Transport studies were carried out using fluorophore-labeled exosomes purified from bovine milk. The transport of bovine exosomes exhibited saturation kinetics at 37°C (K_m= 55.5±48.6 µg/200 µL, V_max= 0.083±0.057 ng exosomal protein x 81,750 cells⁻¹ x …

A Pilot Mixed Methods Evaluation Study Of The Effectiveness Of An After-School Cooking Club Curriculum For Middle School Students, Elisha M. Hall

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Introduction: Obesity has exponentially increased each decade, with approximately one third of the adult American population obese and another one third overweight. More startling is that these trends are rising in children as well. With economic, health, social, mental, and emotional consequences, childhood obesity has quickly risen as a significant issue to tackle. Increases in fast food consumption have been linked with overweight status, and home cooked meals are slowly becoming part of the past. The adolescent time period, in which children are slowly gaining more autonomy and conducting their activities independent from their parents, is an optimal time …

Assessment Of The Nutrition And Physical Activity Education Needs Of Child Care Providers Across Nebraska, Tracy C. Delaney

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Studies estimate 82% of children less than six years of age in the United States are enrolled in non-parental care outside of their home (1). In Nebraska 4,106 child care providers hold licensure (2). This unique child care environment offers opportunity to address food related challenges facing young children. The purpose of this study is to assess the nutrition and physical activity practices provided for children, self-efficacy, and perceived needs of child care providers across Nebraska. A gap in knowledge exist for practices of child care providers in Nebraska and the impact of Child and Adult Care Food Program (CACFP), …

Attitudes Of College Students In Relationship To Weight And Physical Activity, Emily N. Estes

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Abstract

BACKGROUND: There is a limited amount of knowledge of the impact current and goal weights of college students have on their attitudes surrounding weight and physical activity.

OBJECTIVE: To examine weight/body image and physical activity attitudes of students and whether those attitudes correlate current and goal weights of students.

SETTING: The questionnaires utilized were completed between the years 2006-2011 at the University of Nebraska—Lincoln.

PARTICIPANTS: Male and female students from the University of Nebraska—Lincoln.

RESULTS: The mean BMI of all participants (n=2255) was 23.8 ± 4.1; for females (n=1403) 23.0 ± 4.0; and for males (n=852) 24.9 ± …

School Food Environment The Frontline For Childhood Obesity Prevention: A Mixed-Method Study Of Nutritional Competencies And Skills Of School Nutriiton Professionals In Nebraska, Zainab Rida

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

The purpose of this statewide study was to address the nutrition knowledge, attitudes, and perceptions of school foodservice personnel in Nebraska regarding offering/serving healthy school meals. Moreover, this study identified some potential barriers and avenues of action for offering/serving healthy school meals. Data obtained from this study indicates that there is a strong correlation (r= .103, p r .237, p< .01) between the foodservice staff’s self-efficacy and their practices of offering/serving healthy school meals. Fortunately, the relationship between foodservice staff practices of offering/serving healthy school meals and their self-efficacy was positive and significantly predicted practices scores, β =.237, P< 0.01. The finding of the present study also identifies many barriers including lack of time and support that face the foodservice personnel in offering/serving healthy school meals. The findings suggested that there is an urgent need of a full school approach to promote and encourage healthy eating habits among students. Future research is needed to evaluate school wellness policies regarding healthy eating practices in schools. Moreover, establish partnerships with communities and universities for intervention that target students and their parents.

Advisor: Wanda Koszewski

Impact Of The Home Food Environment On Dietary Intake, Obesity And Cardiovascular Health Of U.S. Children And Adolescents, Aged 6-19, Melissa A. Masters

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Youth obesity is an ongoing problem in the United States. Obese children and adolescents are likely to be obese as adults and have an increased risk of developing chronic diseases, including coronary heart disease, earlier in life. The multifactorial nature of obesity continues to challenge researchers and health professionals to determine methods for preventing and reducing childhood obesity. Research has suggested that obesity is a normal response to an “obesigenic” environment. Emerging as one of the most influential environments in obesity and behavior development is the home food environment. However, little is understood about the role of the home food …

Evaluation Of A Social Marketing Campaign: 4 Day Throw Away, Katie J. James

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Children are at a heightened risk for foodborne illness. Handling leftover food safely was identified as a concern from focus groups with the main food preparer in families with young children in two Midwestern states. To design, implement, and evaluate a theory-based food safety education intervention to raise awareness of the USDA guideline for handling leftover food. The food safety social marketing campaign “4 Day Throw Away” launched in towns throughout Nebraska and Iowa. The campaign included both traditional and social media methods to relay the message of discarding leftovers after four days. A total of 600 guardians of young …

Dietitian’S Problem Solving Knowledge To Promote And Support Breastfeeding, Cassandra L. Dytrych

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

As a result of ongoing efforts in the U.S. to promote breastfeeding as the normal way to nourish an infant, breastfeeding initiation rates are higher than they have been in decades. As the experts on food and nutrition through the lifecycle, registered dietitians (RDs) should be knowledgeable to promote and support breastfeeding. It is not well known if RDs have this knowledge. The purpose of this study was to determine the current knowledge of RDs about breastfeeding, the types of professional opportunities RDs have to promote and support breastfeeding, and the level of education RDs have to deal with breastfeeding …

Identification And Assessment Of Markers Of Biotin Status In Healthy Adults, Wei Kay Eng

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Human biotin requirements are unknown and the identification of reliable markers of biotin status is necessary to fill this knowledge gap. Here we used an outpatient feeding protocol to create states of biotin deficiency, sufficiency, and supplementation in 16 healthy men and women. Twenty possible markers of biotin status were assessed including the abundance of biotinylated carboxylases in lymphocytes, the expression of genes from biotin metabolism, and the urinary excretion of biotin and organic acids. Only the abundance of biotinylated 3-methylcrotonyl-CoA carboxylase (holo-MCC) and propionyl-CoA carboxylase (holo-PCC) allowed for distinguishing among all three levels of biotin intake. The urinary excretion …

Breakfast Consumption Habits Of Youth Living In Low Income Neighborhoods In A Large Three County Metropolitan Area, Elijah Dacy

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Purpose: The purpose of this study was to identify youth attitudes, frequency of, and barriers for breakfast consumption and the use of available nutrition programs by youth living in poverty or food insecure households in a large three county metropolitan area. Methods: A phone survey was developed and tested in order to collect information on breakfast consumption habits and attitudes of children, targeted as food insecure. There were no incentives for persons to participate in the survey and all information gathered was kept in a secure place at the Center for Human Nutrition. Participants who were allowed to …

Mixed Methods Analysis Of School Wellness Programs In Nebraska And Indiana: A Descriptive Study, Jessica A. Robinson

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Schools have become one of the major fronts that the battle against childhood obesity is being fought. In 2004 the Child Nutrition Act passed by Congress required all school districts receiving funds for the National School Lunch Program to create a wellness policy. Since then there have been many research projects conducted to learn more about these policies, and their implementation as programs in the schools. Thus far, no such research has been done in Nebraska or Indiana. The purpose of this descriptive study is to describe how schools in Nebraska and Indiana are implementing wellness programs in their schools. …

Assessment Of Nutrition Knowledge And Self-Efficacy Of Ncaa Athletes, Melissa M. Wallinga

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

BACKGROUND Little data has been collected on the nutrition knowledge and self efficacy of NCAA Athletes, and if Sports Nutrition Departments at Universities do positively affect the nutrition knowledge and self-efficacy of athletes. OBJECTIVES To determine and compare differences in nutrition knowledge and self-efficacy of athletes at two NCAA Division I Universities, one with a Sports Nutrition Department, and one without.

SETTING Participants completed a survey in person at their respective University.

PARTICIPANTS Male and female student athletes (n=327) from two Division I Universities, participating in men’s football, men’s wrestling, women’s soccer, or women’s swimming/diving.

RESULTS Approximately 42% of the …

Perceived Importance And Actual Intake Of Calcium And Vitamin D In Young Female Athletes, Miriam E. Zambrano

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Calcium needs increase as a child grows. Actual intake often decreases as a child gets older, leaving his/her developing bones at risk for injury during childhood and increasing the risk of developing osteoporosis later in life. The purpose of this study was to investigate relationships between psychosocial factors influencing consumption of calcium and vitamin D-rich foods and actual intakes of these nutrients. We examined the dietary calcium and vitamin D intakes, determined serum vitamin D levels, and assessed nutrition knowledge and perception of a sample of 20 young girls participating in competitive cheerleading in Omaha, Nebraska. From this information we …

Effects Of Single Nucleotide Polymorphisms In The Human Holocarboxylase Synthetase Gene On Catalytic Activity, Shingo Esaki

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Holocarboxylase synthetase (HCS) catalyzes the covalent binding of biotin to carboxylases and histones in eukaryotic cells. Biotinylated carboxylases play essential roles in the metabolism of fatty acids, amino acids, and glucose; biotinylated histones play essential roles in gene regulation and genome stability. HCS null individuals are not viable whereas HCS deficiency is linked to developmental delays and phenotypes such as short life span and low stress resistance. Greater than 2,500 single nucleotide polymorphisms (SNPs) have been reported for HCS, but the biological importance of these polymorphisms is unknown. We hypothesized that some of these SNPs impair catalytic activity and that …

Intervention To Increase Knowledge And Consumption Of Folate-Rich Foods Based On The Health Belief Model, Lindsay M. Labrosse

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Folate is important in the prevention of birth defects and in the maintenance of general health. Even after mandatory fortification, many people are still not consuming the RDA of 400 mcg/day. Consuming a diet rich in naturally high-folate foods, as opposed to supplementation, may offer additional health benefits and promote an overall healthy diet.

The purpose of this pilot study was to test the hypothesis that a learner-centered educational intervention based on the Health Belief Model (HBM) will successfully increase knowledge and consumption of folate-rich foods, while increasing positive beliefs about folate and health. A two group parallel control trial …

Farm To School: A Case Study Of Four Rural Nebraska Schools Before Initiating Locally Grown Foods In School Food Service, Morgan Swisher

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

More than 25% of children in the U.S. are overweight. Farm to School is a program that encourages schools to procure local produce and nutrition education in an effort to improve childhood nutrition. A case study involving four schools has examined the attitudes of food service managers, food service staff members, and educators who are about to incorporate this program into their schools. Food service managers were interviewed about the foods they intend to purchase, food safety, menu planning, expected costs and receiving, additional training, and staff interest. From the qualitative study, the following themes were found: “More work for …

A New Single Workbout Test To Estimate Critical Power And Anaerobic Work Capacity, Haley Bergstrom

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

The purpose of the present study was to develop a new 3-min, single workbout test to estimate critical power (CP) and anaerobic work capacity (AWC) using the Monark cycle ergometer with the resistance based on the individual’s body weight. Twelve moderately-trained adults (6 men and 6 women; mean age ± SD = 23.2 ± 3.5 yr) performed an incremental cycle ergometer test to exhaustion. The CP and AWC were estimated from the original work limit (W_lim) versus time limit (T_lim) relationship (CP_PT) and a 3-min all-out test (CP_3min) against a fixed resistance …

Intent Of Expecting Fathers To Encourage Breastfeeding, Perceptions Of Support And Barriers To Encouraging Breastfeeding, Katrina Harwood

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Breastfeeding is well known to be the optimal feeding method for healthy infants. Although the benefits of breastfeeding have been well documented, according to the Centers for Disease Control (CDC) 2010 breastfeeding report card, breastfeeding rates at 3months, 6 months and 12 months post-partum have fallen below the objectives set by Healthy People 2010. A mother’s decision to breastfeed has been associated with a variety of factors including, income, education level, geographic location and level of breastfeeding support provided by health care professionals, her mother and the infant’s father. The father of the unborn child has been identified as being …

Regulation Of Sterol Transport By Dietary Phytosterol Esters, Trevor J. Carden

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

LDL cholesterol is associated with the development of atherosclerosis and is therefore considered an important target for intervention to prevent cardiovascular diseases. The inhibition of cholesterol absorption in the small intestine is an attractive approach to lowering plasma cholesterol, one that is exploited by drug therapy as well as dietary supplementation with plant sterols. The mechanism of action of plant sterol esters (PSE) is still incompletely understood, therefore this study was conducted to test the hypothesis that hydrolysis of plant sterol esters is necessary for their cholesterol-lowering effects to be realized.

Male Syrian hamsters were fed diets containing no PSE, …

Omega-3 Dietary Intakes, Knowledge, And Attitudes In Patients Screened For Colon Cancer, Cindy Kaminski

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Objective: The purpose of this study was to estimate Omega-3 (n-3) fatty acid intakes in colonoscopy patients and to examine knowledge and attitudes held about dietary fats.

Subjects/Methods: Men (n=32) and women (n=47) patients were recruited from a Midwestern gastroenterology clinic. A dietitian obtained informed consent, a food recall and response from a 152-item n-3 food frequency questionnaire (n-3 FFQ) at the clinic using visuals and food models. Two additional 24-h recalls, including a weekend recall, were returned by mail. A questionnaire measuring diet fatty acids knowledge, diet attitudes and beliefs was also obtained.

Results: Daily intake of n-3 fatty …

Preschool Children’S Willingness To Try And Preference For Docosahexaenoic Acid Omega-3 Foods, Bethany Joann Bettenhausen

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

During the preschool years, obesigenic eating habits are formed which are strongly associated with risk of developing chronic diseases later in life particularly cardiovascular disease. Docosahexaenoic acid (DHA) omega-3 fatty acid is a polyunsaturated fat with known benefits for heart health, brain cell structure, and retinal development. Current intakes of DHA are below recommended levels. United States (US) Midwestern children may be particularly at risk for suboptimal intakes of DHA. However, foods fortified with life’sDHA^TM, a product of Martek Biosciences and omega-3 eggs may provide culturally acceptable sources of DHA to Midwestern children.

Very little is known about …

Sensory Evaluation Of Ice Cream Made With Prebiotic Ingredients Substituted For Sugar, Jennifer M. Wood

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Prebiotics are non-digestible food ingredients that beneficially affect the host by selectively stimulating growth and/or activity of beneficial bacteria in the colon. The project objective was to determine consumer acceptability of ice cream with prebiotic ingredients substituted for part of the sugar and to determine sensory attributes of sweetness, smoothness, and vanilla flavor. A commercial ice cream mix was made substituting 0%, 10%, 20%, or 30% of the sugar for either Fructooligosaccharides (FOS) or inulin. Sensory analyses were conducted using 95 non-trained panelists. Overall consumer acceptability and sensory attributes were measured on a 175 mm anchored hedonic scale. When 10% …

Mechanisms Of Decreased Cholesterol Absorption Mediated By Phytosterols In The Intestinal Lumen, Andrew W. Brown

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Phytosterols and their fatty acyl esters have been known for decades to lower LDL cholesterol, making them powerful nutraceuticals in lowering cardiovascular disease risk. The mechanisms by which phytosterols lower cholesterol, though, have been incompletely characterized. Three studies were executed to examine three aspects of cholesterol and phytosterol interactions in the intestinal lumen. In the first study, the ability of pancreatic cholesterol esterase to hydrolyze phytosterol esters was examined. Pancreatic cholesterol esterase hydrolyzed phytosterol esters, but the rate of hydrolysis proved sensitive to the structures of both the sterol and ester components. In the second study, cholesterol micellarization was challenged …

Qualitative Study To Determine Quality Of Life Factors Based On Reported Efnep Sucess Stories, Megan L. Hlavacek

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

The purpose of this study was to develop an understanding of how adult participants describe quality of life during or after participation in the Expanded Food and Nutrition Education Program (EFNEP) at sites across the United States. The study analyzed success stories submitted by EFNEP staff and participants from sites in nine states from the years 2004 to 2010. A total of 1,057 success stories were analyzed.

The success stories were descriptions of the participants’ experiences with EFNEP. The stories were analyzed by highlighting words, phrases, and/or sentences, and creating a short one to three word codes that would best …

A Pilot Study Of The Relationship Of Calcium Intake And Frequency Of Injuries In High School Athletes, Jeffrey K. Ebert

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Little information is available related to adolescent calcium intake and relationships with injuries they might suffer from sport participation. To determine calcium intake of high school athletes, to assess their self reported injury rates, and to examine the relationship between the two over a 12 month period of time. Participants received a questionnaire at their school and completed it anywhere they found convenient. Adolescent athletes in the Lincoln Public School system (n=43) that participated in at least one sport in the past year. Four age groups participated in the study with sixteen year olds having a significantly higher calcium intake …

Regulation Of Atp Binding Cassette Transporter A1 And G1 Expression By Fatty Acids, Chai Siah Ku

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

High density lipoprotein (HDL) is an atheroprotective element which is critical in the reverse cholesterol transport (RCT) pathway to eliminate excess cholesterol from the periphery of the body. A low plasma HDL cholesterol levels indicate a higher risk of getting cardiovascular diseases (CVD). Formation and metabolism of HDL largely rely on ATP binding cassette transporter A1 (ABCA1) and G1 (ABCG1) that are transporters to facilitate the efflux of cellular cholesterol to outside of cells. ABCA1 in the liver and small intestine is the major contributing factor for HDL formation. On the other hand, ABCA1 and ABCG1 present in macrophages contribute …

A Qualitative Analysis Of Youth Feedback Of Nutrition School Enrichment Kits In Lincoln, Ne, Richard A. Losey

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

A Qualitative Analysis of Youth Feedback of Nutrition School Enrichment Kits in Lincoln, NE Richard A. Losey, M.S. University of Nebraska, 2010 Advisor: Wanda M. Koszewski The qualitative analysis of the responses given by students participating in classrooms that used the Nutrition Education Program‟s (NEP) school enrichment kits was the primary focus of this research. Data was collected from the participants in written form. Three major themes appeared during analysis of the data, healthy eating habits, cleanliness and change. The theme of healthy eating habits is comprised of the following sub-themes: breakfast, nutrients, label reading, Food Guide Pyramid/MyPyramid and healthy …

A Pilot Study To Determine The Effectiveness Of Probiotic Use In Elderly Patients With Antibiotic-Associated Diarrhea, Jenna L. Peate

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Long-term care settings have the majority of their patients on multiple antibiotics, and outbreaks of antibiotic-associated diarrhea and Clostridium difficile are common. Probiotics have been used with these patients to reduce these side effects. Probiotics can re-establish the composition of intestinal microflora, enhance immune response, and clear pathogens from the host which may reduce the symptoms of antibiotic-associated diarrhea. Therefore, the goal of this study was to conduct a retrospective study of the effectiveness of using probiotic in elderly patients in a long-term care facility in a Midwestern city who suffered from antibiotic-associated diarrhea. The probiotic, CulturelleTM had been administered …