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Effect Of Curcumin On Tnf-Α-Induced Cyclooxygenase-2 Gene Expression And Prostaglandin E2 Synthesis In Bone Marrow Stromal Cell Line, Varittha Thongtan

Chulalongkorn University Theses and Dissertations (Chula ETD)

The objective of this study was to investigate the effect of curcumin on TNF-α-induced expression of COX-2 and PGE2 synthesis in mouse ST2 bone marrow-derived stromal cells. ST2 cells were treated with TNF-α to determine the optimum concentration and treatment duration for inducing COX-2 expression. Then, ST2 cells were treated with TNF-α in the absence or presence of various concentration of curcumin. COX-2 expression was determined using reverse transcriptase polymerase chain reaction and PGE2 level in culture medium was measured using ELISA. TNF-α at concentrations of 1-50 ng/mL significantly increased COX-2 expression, with the maximum induction at 20 ng/mL. The …