Oral Biology and Oral Pathology Commons^™

Establishment And Characterization Of Asian Oral Cancer Cell Lines As In Vitro Models To Study A Disease Prevalent In Asia, Hamid S., Lim K. P., Rosnah Binti Zain, Ismail S. M., Lau S. H., Mustafa W. M. W., Abraham M. T., Nam N. A., Teo S. H., Cheong S. C.

Prof. Dr. Rosnah Binti Zain

We have established 3 cell lines ORL-48, -115 and -136 from surgically resected specimens obtained from untreated primary human oral squamous cell carcinomas of the oral cavity. The in vitro growth characteristics, epithelial origin, in vitro anchorage independency, human papillomavirus (HPV) infection, microsatellite instability status, karyotype and the status of various cell cycle regulators and gatekeepers of these cell lines were investigated. All 3 cell lines grew as monolayers with doubling times ranging between 26.4 and 40.8 h and were immortal. Karyotyping confirmed that these cell lines were of human origin with multiple random losses and gains of entire chromosomes …