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Full-Text Articles in Oral Biology and Oral Pathology
Sociodemographic Characteristics, Clinicopathological Parameters And Survival Among Malaysians With Oral Squamous Cell Carcinoma (Oscc), Ghani W. M. N., Rosnah Binti Zain, Jalil N., Razak I. A., Raja-Latifah R. J., Samsuddin A. R.
Sociodemographic Characteristics, Clinicopathological Parameters And Survival Among Malaysians With Oral Squamous Cell Carcinoma (Oscc), Ghani W. M. N., Rosnah Binti Zain, Jalil N., Razak I. A., Raja-Latifah R. J., Samsuddin A. R.
Prof. Dr. Rosnah Binti Zain
Introduction: Western researchers showed that oral squamous cell carcinoma (OSCC) patients exhibit lower survival rate and poorer prognosis compared to other type of cancer where several clinicopathological parameters have been implicated in the survival of patients. This study aims to assess the association between sociodemographic and clinicopathological parameters with survival of OSCC patients. Method: Data were collected from 253 histologically diagnosed OSCC patients attending selected clinics nationwide. Survival was analyzed for relation with habits, site, disease stage and nodal involvement. Results: Mean age was 58.9 ± 12.6 years with 8.3% aged less than 40. Majority is females (61.1%) and of …
Mdm2 Splice Variants In Oral Cancer, Rosnah Binti Zain
Mdm2 Splice Variants In Oral Cancer, Rosnah Binti Zain
Prof. Dr. Rosnah Binti Zain
Introduction: MDM2 is a negative regulator of the p53 tumor suppressor protein. The presence of a variety of MDM2 splice variants have been reported in many different types of tumor and some have been shown to be associated with patient prognosis.Materials and methods: In this study, we demonstrated the occurrence of MDM2 splice variants in OSCC tissues. RNA was extracted from 45 OSCC tissues and 17 normal oral mucosa tissues and reverse transcribed into cDNA. Nested PCR was performed to amplify the MDM2 transcripts and the PCR amplicons were cloned into cloning vector pTZ57R/T and sequenced.Results: We demonstrated that 37/45 …
Microrna Profiles Of Oral Squamous Cell Carcinoma (Oscc) Using Formalin-Fixed, Paraffin Embedded (Ffpe) Tissue, Siow M. Y., Ng L. P. K., Abraham M. T., Zaini Z. M., Cheong S. C., Rosnah Binti Zain
Microrna Profiles Of Oral Squamous Cell Carcinoma (Oscc) Using Formalin-Fixed, Paraffin Embedded (Ffpe) Tissue, Siow M. Y., Ng L. P. K., Abraham M. T., Zaini Z. M., Cheong S. C., Rosnah Binti Zain
Prof. Dr. Rosnah Binti Zain
Introduction: MicroRNAs (miRNAs) have previously found to be highly tissue- or disease-specific biomarkers with clinical applicability. However, the role of miRNAs in OSCC progression has yet to be well-studied. Here, we demonstrated the use of FFPE specimens in microarray profiling and quantitative real-time PCR (qPCR) approaches to identify miRNAs that are differentially expressed in OSCC compared with non-tumour tissues.Materials and methods: Four OSCC and four non-tumour FFPE tissues were obtained from the Malaysian Oral Cancer Database and Tumour System (MOCDTBS), coordinated by OCRCC-UM and profiled using Agilent 8 × 15 k mammalian miRNA microarray. Based on microarray results, TaqMan miRNA …
Immunohistochemical Features Of Extra-Nodal Nk/T Cell Lymphoma, Muhamad H. M., Ibrahim N. I., Rosnah Binti Zain
Immunohistochemical Features Of Extra-Nodal Nk/T Cell Lymphoma, Muhamad H. M., Ibrahim N. I., Rosnah Binti Zain
Prof. Dr. Rosnah Binti Zain
NK/T cell lymphoma is a destructive extranodal lymphoproliferative disorder of a putative natural killer (NK) cell lineage. This rare malignant lymphoma is difficult to diagnose due to the histological resemblance to reactive processes hence was once considered as an inflammatory lesion known as lethal midline granuloma or non-healing granuloma. Providentially, the advance in immunophenotyping has permit characterization of NK/T-cell lymphoma, as a distinct entity from other type of lymphoma. It is most commonly involved the nasal cavity, paranasal sinuses and hard palate and as the case we reported here of a NK/T-cell lymphoma in a 29 year old Indonesian man …
High Throughput Mutational Profiling Of Signaling Molecules In Oral Cancer, Zanaruddin , S. N. S., Cheong , S. C., Rahman , Z. A. A., Abd Ghani , W. M. N. W, Mustafa , W. M. W., Rosnah Binti Zain, Kong , Y. H., Hor , S. Y., Teo , S. H.
High Throughput Mutational Profiling Of Signaling Molecules In Oral Cancer, Zanaruddin , S. N. S., Cheong , S. C., Rahman , Z. A. A., Abd Ghani , W. M. N. W, Mustafa , W. M. W., Rosnah Binti Zain, Kong , Y. H., Hor , S. Y., Teo , S. H.
Prof. Dr. Rosnah Binti Zain
It is well established that a plethora of mutations that occur in oncogenes and tumor-suppressor genes are the main drivers of tumorigenesis. The identification of these mutations provides important clues to the cellular processes of carcinogenesis and affords an opportunity for these genes to be used for diagnostic and therapeutic purposes. In comparison to cancers such as breast, lung and colorectal, there is a lack of systematic, large scale characterization of oncogenic mutations oralsquamous cell carcinoma (OSCC).
Genome Wide Profiling Of Tongue And Cheek Cancer Using High Resolution Array Based Cgh, Chong V. K. V., Rahman Z. A. A., Zakaria Z., Tay K. K., Ismail S. M., Rosnah Binti Zain
Genome Wide Profiling Of Tongue And Cheek Cancer Using High Resolution Array Based Cgh, Chong V. K. V., Rahman Z. A. A., Zakaria Z., Tay K. K., Ismail S. M., Rosnah Binti Zain
Prof. Dr. Rosnah Binti Zain
Introduction: Tongue and cheek cancer have different behaviors. In order to understand these behaviors, there is a need to look into the chromosomal alterations and gene pathways that maybe associated with oral cancer at these sites. Therefore, the objective of this study is to determine the differences in chromosomal aberrations and gene pathways involved in tongue and cheek cancer using high resolution array CGH. Methods: A genome wide screening with array CGH (SurePrint G3 CGH 1x1M microarray) was performed using gDNA from 20 snap frozen fresh tissues consisting of 12 tongue and 8 cheek oral squamous cell carcinomas (samples from …
Expression Of Gna12 And Its Role In Oral Cancer, Gan C. P., Rosnah Binti Zain, Abraham M. T., Patel V., Gutkind J. S., Cheong S. C., Chong C. E., Hamid S., Teo S. H.
Expression Of Gna12 And Its Role In Oral Cancer, Gan C. P., Rosnah Binti Zain, Abraham M. T., Patel V., Gutkind J. S., Cheong S. C., Chong C. E., Hamid S., Teo S. H.
Prof. Dr. Rosnah Binti Zain
Introduction: The variability of clinical outcomes in oral cancer patients and the heterogeneity of the disease are the main challenges for the improvement of current treatment modalities. Efforts in our laboratory have focused on the molecular profiling of oral cancer to understand the mechanisms underlying the disease. In a previous microarray study, we found Guanine nucleotide binding protein alpha-12 (GNA12) to be up-regulated in oral cancer. Materials and methods: In this study, we validated the expression of GNA12 at the mRNA level in 47 oral squamous cell carcinoma (OSCC) and 18 non-malignant oral mucosa tissues, by quantitative polymerase chain reaction …
Dietary Consumptions Pattern And Risk Of Oral Cancer: A Malaysian Scenario, Helen-Ng L. C., Ishak I. A., Ghani W. M. N., Marhazlinda J., Norlida A., Rosnah Binti Zain
Dietary Consumptions Pattern And Risk Of Oral Cancer: A Malaysian Scenario, Helen-Ng L. C., Ishak I. A., Ghani W. M. N., Marhazlinda J., Norlida A., Rosnah Binti Zain
Prof. Dr. Rosnah Binti Zain
Introduction: The risk of cancer has been extensively investigated with the intake of single food items but food consumption in an individual is usually complex, made up by combinations of different food items. This study aims to identify dietary patterns of Malaysians, and establishing relationship between dietary pattern and oral cancer risk. Methods A hospital-based, case-control study was conducted on 300 Malaysians who seek treatment at participating hospitals between January 2003 and December 2010. The subjects consisted of 150 cases and 150 controls that were matched for gender, age (5 years) and ethnicity. Dietary intake was measured using Food Frequency …
Effects Of Smoking On Oral Cancer Transcriptome, Rosnah Binti Zain, Karen-Ng L. P., Cheong S. C., Anwar A., Mustaffa W. M. W., Prepagaran N., Zaini Z., Merican A. F., Abraham M. T., Tay K. K., Rahman Z. A. A., Jallaludin A.
Effects Of Smoking On Oral Cancer Transcriptome, Rosnah Binti Zain, Karen-Ng L. P., Cheong S. C., Anwar A., Mustaffa W. M. W., Prepagaran N., Zaini Z., Merican A. F., Abraham M. T., Tay K. K., Rahman Z. A. A., Jallaludin A.
Prof. Dr. Rosnah Binti Zain
Introduction: Oral cancer is a debilitating disease and the survival rates for these patients have not improved over the past decades. Tobacco smoking is one of the most common risk factor associated with oral cancer. Objectives: To explore and identify differential genes expression associated with tobacco smoking. Methodology: Next generation sequencing using the Illumina Genome Analyzer was done to sequence five fresh frozen oral cancer tissue samples from smoking patients, two of which were former smoker and an additional 8 samples consisting of normal oral mucosal tissues from the alveolar mucosa of non-cancer patients where 4 were smokers and another …
Combined Effects Of Isothiocyanates (Itcs) Intake, Glutathione S-Transferases (Gsts) Polymorphism And Risk Habits On Oral Squamous Cell Carcinoma (Oscc) Associated With Earlier Age Of Disease Onset, Karen-Ng L. P., Marhazlinda J., Rahman Z. A. A., Abraham M. T., Cheong S. C., Rosnah Binti Zain
Combined Effects Of Isothiocyanates (Itcs) Intake, Glutathione S-Transferases (Gsts) Polymorphism And Risk Habits On Oral Squamous Cell Carcinoma (Oscc) Associated With Earlier Age Of Disease Onset, Karen-Ng L. P., Marhazlinda J., Rahman Z. A. A., Abraham M. T., Cheong S. C., Rosnah Binti Zain
Prof. Dr. Rosnah Binti Zain
Introduction: ITCs found in cruciferous vegetables has been reported to reduce cancer risk by inducing phase II conjugating enzymes, in particular GSTs. Interestingly, these enzymes also metabolize ITCs therefore; the protective effects of ITCs would depend on the activity of GSTs. This study aimed to determine association between dietary ITCs, GSTs polymorphisms and risk habits (cigarette smoking, alcohol drinking and betel-quid chewing) with oral cancer.Method: Included in this study were 115 OSCC patients and 116 healthy subjects. Information on ITC intake from cruciferous vegetables was collected via a semi-quantitative FFQ. Peripheral blood lymphocytes were obtained for genotyping of GSTM1, GSTT1 …
Association Between Retinol And Vitamin C With The Risk Of Oral Cancer - A Case-Control Study, Vimmitra A., Razak I. A., Helen-Ng L. C., Ghani W. M. N., Abdullah N., Rosnah Binti Zain
Association Between Retinol And Vitamin C With The Risk Of Oral Cancer - A Case-Control Study, Vimmitra A., Razak I. A., Helen-Ng L. C., Ghani W. M. N., Abdullah N., Rosnah Binti Zain
Prof. Dr. Rosnah Binti Zain
Introduction: Dietary intake has been hypothesized to be associated with various diseases including oral cancer. According to studies worldwide, imbalanced diet and nutrient deficiency have been attributed to promote oral malignancies. However, the function of specific nutrients in preventing oral cancer remains largely undefined. This study aims to investigate the association between intake of retinol and vitamin C with the risk of oral cancer. Materials and methods: Dietary intake data was collected from 8 participating hospitals throughout Malaysia using a validated foodfrequency questionnaire (FFQ). Intake of retinol and vitamin C were computed using the Nutriemart software. This case-control study included …
Significant Association Of High-Risk Human Papillomavirus (Hpv) But Not Of P53 Polymorphisms With Oral Squamous Cell Carcinomas In Malaysia, Saini R., Tang T. H., Rosnah Binti Zain, Cheong S. C., Musa K. I., Saini D., Ismail A. R., Abraham M. T., Mustafa W. M. W., Santhanam J.
Significant Association Of High-Risk Human Papillomavirus (Hpv) But Not Of P53 Polymorphisms With Oral Squamous Cell Carcinomas In Malaysia, Saini R., Tang T. H., Rosnah Binti Zain, Cheong S. C., Musa K. I., Saini D., Ismail A. R., Abraham M. T., Mustafa W. M. W., Santhanam J.
Prof. Dr. Rosnah Binti Zain
Purpose: The purpose of this study was to evaluate the role of HPV and p53 polymorphisms in oral squamous cell carcinomas (OSCC) affecting Malaysian population. Methods: We analysed frozen samples from 105 OSCC as well as 105 oral specimens derived from healthy individuals. PCR assays targeting two regions of the virus were used. PCR amplification for the analysis of p53 codon 72 arginine/proline alleles was carried out in a separate reaction. Results: HPV DNA was detected in 51.4% OSCC samples, while 24.8% controls were found to be HPV positive. HPV was found to be significantly associated with OSCC (P < 0.001, …
Factors Affecting Commencement And Cessation Of Betel Quid Chewing Behaviour In Malaysian Adults., Ghani W. M. N., Razak I. A., Yang Y. H., Talib N. A., Ikeda N., Axell T., Gupta P. C., Handa Y., Abdullah N., Rosnah Binti Zain
Factors Affecting Commencement And Cessation Of Betel Quid Chewing Behaviour In Malaysian Adults., Ghani W. M. N., Razak I. A., Yang Y. H., Talib N. A., Ikeda N., Axell T., Gupta P. C., Handa Y., Abdullah N., Rosnah Binti Zain
Prof. Dr. Rosnah Binti Zain
Background. Betel quid chewing is a common habit widely practiced in Southern Asian populations. However, variations are seen in the content of a betel quid across the different countries. Factors associated with commencement and cessation of this habit has been numerously studied. Unfortunately, data on Malaysian population is non-existent. This study aims to determine the factors associated with the inception and also cessation of betel quid chewing behaviour among Malaysian adults. Method. This study is part of a nationwide survey on oral mucosal lesions carried out among 11,697 adults in all fourteen states in Malaysia. The questionnaire included sociodemographic information …
Genetic Polymorphisms And Risk Of Oral Cancer, Zaini Z. M., Karen-Ng L. P., Cheong S. C., Gurdeep S. M., Yuen K. M., Tay K. K., Jalil N., Ismail S. M., Jallaluddin A., Rosnah Binti Zain
Genetic Polymorphisms And Risk Of Oral Cancer, Zaini Z. M., Karen-Ng L. P., Cheong S. C., Gurdeep S. M., Yuen K. M., Tay K. K., Jalil N., Ismail S. M., Jallaluddin A., Rosnah Binti Zain
Prof. Dr. Rosnah Binti Zain
Objectives: This study was done to investigate the role of single nucleotide polymorphisms(SNPs) within genes of phase I (CYP1A1) and phase II (GSTM1, GSTT1,GSTP1) of the xenobiotic metabolism and its association with oral cancer risk.Methods: An unmatched case-control study was conducted using 207 newly diagnosed oral cancer patients and 117 non-cancer subjects selected from the OCRCC database. Peripheral blood was obtained from consented individuals and the CYP1A1, GSTM1, GSTT1 and GSTP1 genotypes were determined using polymerase chain reaction (PCR) and restriction enzyme digestion (RFLP). Simple and multiple logistic regression yielding odds ratio (OR and aOR) were employed to measure the …
Selected Transcriptome Profiles Of Oral Cancer Suggestive Of Field Cancerisation Using Second Generation Sequencing, Rosnah Binti Zain, Anwar A., Karen-Ng L. P., Cheong S. C., Zaini Z., Prepagaran N., Hercus R., Idris N. M., Saidin A., Chia J. Y., Merican A. F., Abraham M. T., Tay K. K., Mustaffa W. M. W., Norain A. T., Rahman Z. A. A., Jallaludin A.
Selected Transcriptome Profiles Of Oral Cancer Suggestive Of Field Cancerisation Using Second Generation Sequencing, Rosnah Binti Zain, Anwar A., Karen-Ng L. P., Cheong S. C., Zaini Z., Prepagaran N., Hercus R., Idris N. M., Saidin A., Chia J. Y., Merican A. F., Abraham M. T., Tay K. K., Mustaffa W. M. W., Norain A. T., Rahman Z. A. A., Jallaludin A.
Prof. Dr. Rosnah Binti Zain
Objectives: To characterize differential gene expression between oral cancerous tissues and the normal mucosal tissues of oral cancer patients and oral mucosal tissues of cancer free patients using second generation sequencing. Methods: Five fresh frozen oral cancer tissue samples from smoking patients, with 2 samples of proximal normal were included in this study. An additional 8 samples consisting of normal oral mucosal tissues from the alveolar mucosa of non-cancer patients who had their wisdom tooth removed where 4 were smokers and another 4 were non-smokers was also included. The tissues and socio-demographic information were obtained from the Malaysian Oral Cancer …
Establishment And Characterization Of Asian Oral Cancer Cell Lines As In Vitro Models To Study A Disease Prevalent In Asia, Hamid S., Lim K. P., Rosnah Binti Zain, Ismail S. M., Lau S. H., Mustafa W. M. W., Abraham M. T., Nam N. A., Teo S. H., Cheong S. C.
Establishment And Characterization Of Asian Oral Cancer Cell Lines As In Vitro Models To Study A Disease Prevalent In Asia, Hamid S., Lim K. P., Rosnah Binti Zain, Ismail S. M., Lau S. H., Mustafa W. M. W., Abraham M. T., Nam N. A., Teo S. H., Cheong S. C.
Prof. Dr. Rosnah Binti Zain
We have established 3 cell lines ORL-48, -115 and -136 from surgically resected specimens obtained from untreated primary human oral squamous cell carcinomas of the oral cavity. The in vitro growth characteristics, epithelial origin, in vitro anchorage independency, human papillomavirus (HPV) infection, microsatellite instability status, karyotype and the status of various cell cycle regulators and gatekeepers of these cell lines were investigated. All 3 cell lines grew as monolayers with doubling times ranging between 26.4 and 40.8 h and were immortal. Karyotyping confirmed that these cell lines were of human origin with multiple random losses and gains of entire chromosomes …
Histopathologic Findings And Assessment Of Cervical Lymph Node Metastasis In Oral Squamous Cell Carcinoma Of Malaysian Patients, Ismail S. B., Rosnah Binti Zain, Kipli N. P., Abraham T., Ismail M. S., Rahman Z. A. A., Prepageran N., Jalaludin M. A. B.
Histopathologic Findings And Assessment Of Cervical Lymph Node Metastasis In Oral Squamous Cell Carcinoma Of Malaysian Patients, Ismail S. B., Rosnah Binti Zain, Kipli N. P., Abraham T., Ismail M. S., Rahman Z. A. A., Prepageran N., Jalaludin M. A. B.
Prof. Dr. Rosnah Binti Zain
Purpose: To evaluate some histopathologic parameters and relate with the occurrence of cervical lymph node metastasis in oral squamous cell carcinoma (OSCC) in Malaysian patients. Methods: A total of 44 cases of OSCC resected were included. The resected specimens were examined for the OSCC type, degree of differentiation, pattern of invasion (at the tumour invasive front), greatest dimension and the depth of stromal invasion. Where there is no neck resection, the clinical nodal status was obtained from the Oral Cancer database maintained at the University of Malaya Oral Cancer Research and Coordinating Centre (UM-OCRCC) while the histopathologic data were obtained …
Expression Of Human B-Defensin-3 In Oral Squamous Cell Carcinoma Using Tissue Microarray - A Preliminary Study, Hussaini H. M., Lim P. K., Rahman R. A., Abraham T., Cheong C. S., Rosnah Binti Zain, Ismail M. S., Nam A. N.
Expression Of Human B-Defensin-3 In Oral Squamous Cell Carcinoma Using Tissue Microarray - A Preliminary Study, Hussaini H. M., Lim P. K., Rahman R. A., Abraham T., Cheong C. S., Rosnah Binti Zain, Ismail M. S., Nam A. N.
Prof. Dr. Rosnah Binti Zain
Purpose: Human b-Defensin (hBDs) is an epithelial origin, cationic antimicrobial peptides. Their expression is reported in oral mucosa, gingiva and salivary glands. However, their protective role is not well understood. It has been suggested that bacterial and viral infection are an important factors in the process of carcinogenesis. This study investigated the expression of hBD-3 in oral squamous cells carcinoma (OSCC). Methods: 29 OSCC and 23 samples of normal and dysplastic tissues were obtained from Oral Pathology diagnostic laboratory, faculty of Dentistry, UKM and UM. The tissues were arrayed and the expression of hBD-3 was analysed using immunohistochemistry technique. Results: …
Immunoexpression Of Prb And Egfr Using Tissue Microarray (Tma) Technique - A Preliminary Study, Kipli N. P., Cheong C. S., Rosnah Binti Zain, Hamid S., Lim K. P., Abraham T., Ismail M. S., Hussaini H. M.
Immunoexpression Of Prb And Egfr Using Tissue Microarray (Tma) Technique - A Preliminary Study, Kipli N. P., Cheong C. S., Rosnah Binti Zain, Hamid S., Lim K. P., Abraham T., Ismail M. S., Hussaini H. M.
Prof. Dr. Rosnah Binti Zain
Purpose: To assess the expression of pRb and EGFR in oral epithelial lesions using TMA technique. Methods: Paraffin embedded specimens were selected from the archives of the Diagnostic Laboratories at the Faculty of Dentistry, University of Malaya and Universiti Kebangsaan Malaysia. Selected epithelial areas were cored and developed into tissue microarray blocks. A total of 32 samples for pRb and 50 samples for EGFR were analysed using immunohistochemical techniques. Results: Abnormal pRb staining (1+ or less) was seen in 80% (8/10) OSCC, 38.5% (5/13) dysplastic and 22.2% (2/9) normal/hyperplastic epithelium. Positive EGFR staining was observed in 25/35 (71.4%) OSCC cases …
Oral Cancer And Precancer Research In Malaysia - The Database And Tissue Resource Bank, Rosnah Binti Zain
Oral Cancer And Precancer Research In Malaysia - The Database And Tissue Resource Bank, Rosnah Binti Zain
Prof. Dr. Rosnah Binti Zain
Introduction: Uncoordinated data collection by different research groups prompted the initiation of Malaysian oral cancer Research Initiative (MOCRI) group. Objectives: To coordinate and standardize data and tissue collection and storage, develop a minimum dataset on risk factors, intervention techniques and quality of life of oral cancer patients. Materials and Methods: A computer software programme is currently being developed to accommodate multi-centre data collection and research activities in eight hospitals. The complete dataset includes parameters on sociodemographic, clinical, pathological, quality of life measures, details of treatment methods, vital status and dietary intake. Tissues are being collected, stored and catalogued as fresh …
Immunohistochemical Localization Of Htert Protein In Oral Carcinogenesis, Kumar S., Ismail S. M., Rosnah Binti Zain, Abraham M. T., Cheong S. C.
Immunohistochemical Localization Of Htert Protein In Oral Carcinogenesis, Kumar S., Ismail S. M., Rosnah Binti Zain, Abraham M. T., Cheong S. C.
Prof. Dr. Rosnah Binti Zain
Introduction: Human telomerase reverse transcriptase (hTERT) the catalytic subunit of telomerase is associated with cellular immortalization and tumorigenesis .In situ detection of hTERT using immunohistochemistry (IHC) allows localizing the hTERT- positive cells and also overcomes the limitation of requirement of fresh tissues for molecular techniques such as Telomeric Repeat Amplification Protocol (TRAP) for telomerase/ hTERT detection. The objectives of this study are to detect hTERT protein expression using immunohistochemistry in different histological stages in oral carcinogenesis with paraffin embedded tissue samples and cultured cells and to relate the hTERT expression with different histological stages in oral carcinogenesis. Materials and Methods: …
Expression Of P53, Prb, Ki-67 And Bcl-2 In Betel Quid Induced Oral Mucosal Lesions, Kipli N. P., Kumar S. K. S., Rosnah Binti Zain, Fan C. Y., Boon P. K.
Expression Of P53, Prb, Ki-67 And Bcl-2 In Betel Quid Induced Oral Mucosal Lesions, Kipli N. P., Kumar S. K. S., Rosnah Binti Zain, Fan C. Y., Boon P. K.
Prof. Dr. Rosnah Binti Zain
Introduction: Alterations in p53, pRb, KI-67 and bcl-2 have been implicated the development of oral cancer although their combined expression in betel quid induced oral mucosal lesions as not clear. The objective of this study is to investigate the expressions of p53, pRb, K1-67 and bcl-2 m oral mucosa of betel quid chewing subjects. Materials and Methods: A total of 64 tissue sections were obtained from biopsy samples of 50 subjects clinically diagnosed with betel chewer's mucosa (BCM, n=32), BCM with leukoplakia (n=12) and leukoplakia (n=6). Oral squamous cell carcinoma (OSCC, 11=10) and normal oral mucosa (n=4) section were used …
Clinical Pathological Evaluation And Risk Factors Of Oral Cancer Cases Of East Coast Of Peninsular Malaysia, Farini M. S., Azlina A., Rushdan I., Manoharan M., Rosnah Binti Zain, Samsudin A. R.
Clinical Pathological Evaluation And Risk Factors Of Oral Cancer Cases Of East Coast Of Peninsular Malaysia, Farini M. S., Azlina A., Rushdan I., Manoharan M., Rosnah Binti Zain, Samsudin A. R.
Prof. Dr. Rosnah Binti Zain
Introduction: Oral cancer is one of the common cancers in Malaysia. Tile population of east coast of Peninsular Malaysia has a different hfestyle and Malay is the predominant race. Oral cancer research in this area started since the Malaysian National Oral Cancer group was established. The aim: of this study is to evaluate the clinical pathological findings and to investigate the role of tobacco smoking, alcohol consurnption and betel quid chewing as tile risk factors among oral cancer cases in east coast of peninsular Malaysia. Materials and Methods: Fifty case records of only treated/operated cases of patients with oral cancer …
Proliferative And Apoptotic Activity At The Tumour Invasive Front In Oral Squamous Cell Carcinoma, Ngeow W. C., Rosnah Binti Zain, Abidin Z., Phaik K. S., Kumar S.
Proliferative And Apoptotic Activity At The Tumour Invasive Front In Oral Squamous Cell Carcinoma, Ngeow W. C., Rosnah Binti Zain, Abidin Z., Phaik K. S., Kumar S.
Prof. Dr. Rosnah Binti Zain
Introduction: the tumour invasive front of squamous cell carcinoma (OSCC) has long been recognized as an important area which harbour prognostic information. The aim of the present study was to describe the expression of K1-67, MDM2 and Bcl-2 at the tumour invasive front in the buccal mucosa and also to investigate whether there is any association between the expression of K1-67, MDM2 and Bcl-2 with the certain sociodemographic characteristics, clinical and histopathological parameters. Materials and Methods: The sample of this study consisted of 31 biopsy cases of OSCC from the buccal mucosa. Immunohistochemistry was used with specific antibodies to K1-67, …
Establishment And Characterization Of An Oral Squamous Carcinoma Cell Line From A Patient With No Apparent Habits Associated With Oral Cancer, Sok C. C., Hamid S., Peng L. K., Ismail S. M., Rosnah Binti Zain
Establishment And Characterization Of An Oral Squamous Carcinoma Cell Line From A Patient With No Apparent Habits Associated With Oral Cancer, Sok C. C., Hamid S., Peng L. K., Ismail S. M., Rosnah Binti Zain
Prof. Dr. Rosnah Binti Zain
Introduction: We have established a cell line ORL-48(T) from a surgically resected specimen of an untreated primary human oral squamous cell carcinona of the mandible.This patient did not appear to have any oral habits which have been reported to be associated with oral cancer Materials and Method: The in vitro growth characteristics, epithelial origin, in vitro anchorage independency, HPV infection, microsatellite instability status, karyotype and the status of various cell cycle regulators and gatekeepers of the ORL-48(T) cell line were investigated. Results: The ORL-48(T) cell line is immortal, 3T3- independent and grew as a monolayer with the doubling time of …
Oral Cancer Incidence In Kelantan, Malaysia, Ghazali N., Rosnah Binti Zain, Samsudin A. R., Rusdi A. R.
Oral Cancer Incidence In Kelantan, Malaysia, Ghazali N., Rosnah Binti Zain, Samsudin A. R., Rusdi A. R.
Prof. Dr. Rosnah Binti Zain
Aim: A pilot study was carried out in the state of Kelantan, north-east of Malaysia, to determine the feasibility of using pathological and hospital databases to acquire incidence on oral cancer. Method: Data on OC patients (ICD-10: C00.3-00.5, C02-06) were obtained from incident surgical biopsy records of the two main referral centres for head and neck surgery in Kelantan namely, the Department of Pathology, Faculty of Medicine, University of Science and the Oral Surgery clinic, Kota Baru Hospital between January 1994 to December 1998. The crude and age-specific incidence rates were calculated based on the annual population census of Kelantan …
Oral Verruco-Papillary Lesions In Malaysia, Ghazali N., Rosnah Binti Zain, Shanmuhasuntharam P., Ghazali M. N., Bakri M. M.
Oral Verruco-Papillary Lesions In Malaysia, Ghazali N., Rosnah Binti Zain, Shanmuhasuntharam P., Ghazali M. N., Bakri M. M.
Prof. Dr. Rosnah Binti Zain
Some oral verruco-papillary lesions (VPL) may constitute part of the clinicopathological spectrum of proliferative verrucous leukoplakia (PVL), an aggressive form of leukoplakia. Previous studies in PVL have documented its existence in Western populations but no data exist from the South-East Asia region. Aim: To review the clinicopathological features of persistent, multifocal oral VPL in a Malaysian population. Method: Patients with multiple biopsies of oral VPL were selected from histopathological records. Seven women and three men with diverse ethnic backgrounds were identified. New sections were made and re-examined by a single pathologist. Results: A total of 45 biopsies were reviewed. The …
Periodontal Status And Treatment Needs Among Indian Estate Population, Ali T. T., Razak I. A., Rosnah Binti Zain
Periodontal Status And Treatment Needs Among Indian Estate Population, Ali T. T., Razak I. A., Rosnah Binti Zain
Prof. Dr. Rosnah Binti Zain
The aim of this study was to determine the periodontal status and treatment needs among the Indian estate population. The Community Periodontal Index of Treatment Needs was used to obtained clinical data on 208 Indian subjects residing in three estates in the state of Selangor. The prevalence of periodontal disease among the subjects was 100%. Less than 1% of the subjects had bleeding on probing as the highest CPITN score. About 97% of the subjects showed evidence of periodontal pocket of 4mm or more. Of these, half showed pocketing of 6mm or more. No statistically significant difference (p> 0.05) was …
Oral Mucosal Lesions In Malay Quid And Non-Quid Chewers In Kelantan, Raman R. A., Rahman Z. A. A., Rosnah Binti Zain, Samsuddin A. R., Razak I. A., Abdullah F., Venugopal I.
Oral Mucosal Lesions In Malay Quid And Non-Quid Chewers In Kelantan, Raman R. A., Rahman Z. A. A., Rosnah Binti Zain, Samsuddin A. R., Razak I. A., Abdullah F., Venugopal I.
Prof. Dr. Rosnah Binti Zain
The Malaysian nationwide survey on oral mucosal lesions (OML) in 1993/94 showed a low prevalence of OML in particular oral cancer and precancer (OPC) among Malays as compared to the Indians and the Indigenous people of Sabah and Sarawak. A low prevalence of quid chewing habit was also noted in the Malays in that survey. The present study was conducted to investigate whether the low prevalence of OPC in the Malays was attributed to the low prevalence of quid users among the Malays and/or whether the quid contents used were different. Two examiners were trained prior to the survey. The …
Clinicopathologic Presentations Of 18 Cases Of Traumatic Granulomas, Rosnah Binti Zain, Ti L. S.
Clinicopathologic Presentations Of 18 Cases Of Traumatic Granulomas, Rosnah Binti Zain, Ti L. S.
Prof. Dr. Rosnah Binti Zain
A retrospective study of 11 cases of traumatic granulomas was done. The objective of this study is to present the clinicopathological features of traumatic granulomas in some malaysian patients and to compare this data with data from other reported studies, which have been predominantly from Caucasian population. Biopsy records from 1985 to 1998 of 18 cases of traumatic granulomas were obtained. There were 13 male and 5 female patients. The granulomas occured in 38.9% Malay, 38.9% Chinese, 11.1% Indian and 11.1% other ethnic groups. The age of these patients range from 8 to 86 years, with mean of 41.9 ± …