Endodontics and Endodontology Commons^™

Intracanal Microbial Reduction After Root Canal Preparation With Different Tapered Instruments. An Ex-Vivo Study, Shehabeldin Mohamed Saber Prof.Dr.

Dentistry

Aim: The objective of this ex vivo study was to assess the dual impact of instruments taper and irrigation activation methods on bacterial reduction in infected root canals.

Materials and methods:42 extracted human molar teeth were inoculated with Enterococcus faecalis for 2 weeks to develop a mature biofilm. The samples were divided into four experimental groups and two control groups. The experimental groups underwent root canal preparation using two different instruments: XP Shaper (taper 0.04) and Reciproc Blue R25 (taper 0.08). Additionally, two different activation methods were tested: mechanical (XP Finisher) and sonic (EDDY). Bacterial reduction was assessed using Colony …

Intracanal Microbial Reduction After Root Canal Preparation With Different Tapered Instruments. An Ex-Vivo Study, Hala Fayek Khalil, Shehab Eldin Moahmed Saber, Mostafa Kholy, Soha Elhady, Esraa Abdelwahab

Dentistry

Aim: The objective of this ex vivo study was to assess the dual impact of instruments taper and irrigation activation methods on bacterial reduction in infected root canals. Materials and methods:42 extracted human molar teeth were inoculated with Enterococcus faecalis for 2 weeks to develop a mature biofilm. The samples were divided into four experimental groups and two control groups. The experimental groups underwent root canal preparation using two different instruments: XP Shaper (taper 0.04) and Reciproc Blue R25 (taper 0.08). Additionally, two different activation methods were tested: mechanical (XP Finisher) and sonic (EDDY). Bacterial reduction was assessed using Colony …

The Effect Of Parabiotic Reuterin On The Expression Of Genes Involved In Candida Albicans Biofilm Formation: An Ex Vivo Study, Anastasya Muna Riad, Armelia Sari Widyarman

Journal of Dentistry Indonesia

Candida albicans has a number of properties, including resistance to various antimicrobial agents, which allow it to survive in the root canals. Lactobacillus reuteri plays a role in maintaining oral health through interactions with the oral microbiome. L. reuteri has potential as a preventive and therapeutic agent against inflammatory diseases. Objective: The aim of this study was to investigate the effect of irrigation with a reuterin-containing solution on BCR1, ACE2, EFG1, and TEC1 gene expression in C. albicans root canal biofilms. Methods: L. reuteri was cultured in MRS broth and incubated anaerobically for 24 hours at 37°C. C. albicans was …

Persistent Endodontics Pathogens Biofilm Inhibited By Lactobacillus Reuteri Indonesian Strain, Armelia Sari Widyarman, Njoo Kiky Elysia Lazaroni

Journal of Dentistry Indonesia

Lactobacillus reuteri is known as probiotics that effective to improve oral health. Objective: To analyze the effect of L. reuteri Indonesian strain, towards Enterococcus faecalis and Candida albicans biofilm growth. Methods: This study was conducted using biofilm assay. Each E. faecalis ATCC-29212 and C. albicans ATCC-10231 were cultured in Brain Heart Infusion Broth, distributed into 96 well-microplate and incubated for 24h, 37°C, anaerobic condition for E. faecalis and aerobic condition for C. albicans. L. reuteri LC382415 (Indonesian strain) was cultured in deMann Rogosa Shar pe Broth and diluted into different concentrations (10⁸,10⁶, and 10^{4 …}

A Comparative Study Of Intraradicular Enterococcus Faecalis Biofilm Removal With Three Root Canal Treatment Systems: A Scanning Electron Microscopy Evaluation, Cyrous Ardalan

Theses and Dissertations

The objective of this study was to evaluate the biofilm removal efficacy of three root canal treatment systems: ProUltra® PiezoFlow™, traditional needle irrigation, and the GentleWave® system in an ex-vivo benchtop study. Twenty-four extracted maxillary and mandibular molars were selected. Teeth were all instrumented to a master apical file size #25 with 4% taper. Teeth were then randomly divided into four experimental groups and two control groups. The root canals were inoculated with a culture of Enterococcus faecalis and incubated for five weeks to form a biofilm. Each group was then treated with one of the different root canal treatment …

Contamination Of Dental Waterlines: Efficacy Of Seven Waterline Treatments And Three In-Office Bacteria Test Kits, Adam Davis

Theses and Dissertations

This study compared seven dental unit water line (DUWL) treatments and three in-office bacteria test kits. Sodium hypochlorite (NaOCl) 1:10 in tap water weekly; 3 drops of NaOCl in 1 liter of water; Dentapure® DP 40; ICX™ tablet; Sterilex® Ultra powder; Lines™; and Selective Micro® Dental-Clean. Traditional culture technique was compared to HPC Dental Sampler; Aquasafe™ Dental Unit Water Line Test Kit; and Bacteria in Water Test Kit. Eight dental units in the Virginia Commonwealth University Graduate Endodontic Clinic were randomly assigned treatment regimens. Samples were taken weekly initially and after flushing for 1 minute. In conclusion NaOCl hypochlorite 1:10 …