Endodontics and Endodontology Commons^™

A Multi-National Questionnaire-Based Analysis Of Dental Students’ Knowledge Of The Management Of Deep Caries And The Exposed Pulp, Venkateshbabu Nagendrababu, Arindam Dutta, Ana Arias, Aleksandar Jakovljevic, Ahmed Hieawy, Frank C. Setzer, Jugoslav Ilic, Milos Beloica, Meric Karapinar Kazandag, Nandini Suresh, Paul V. Abbott, Raghavendra M. Shetty, Srinivasan Narasimhan, Victoria Sh Yu, Vellore Kannan Gopinath, Ya Shen, Henry F. Duncan

School of Dentistry Faculty Publications

Introduction and aims: To evaluate knowledge regarding the management of deep carious lesions and exposed pulps among undergraduate and postgraduate endodontic students from ten dental institutions across ten countries, and the impact of operator (material, antibiotic prescription) and patient-related (age, symptoms) factors on their treatment protocols. Methods: An online questionnaire was distributed to evaluate student knowledge of the management of deep caries and exposed pulp related to four clinical scenarios. Simple descriptive statistics were used to describe the data and McNemar tests were employed to identify significant differences between the scenarios. The P-value was set at 5%. Results: A total …

Retreatability Of Bioceramic And Resin-Based Root Canal Sealers Using Xp Shaper Rise: An In Vitro Study, Muhammed Ahmed Omar Abouel Seoud Phd, Shehab Saber

Dentistry

Aim: The purpose of this study was to investigate in-vitro the retreatability of premixed calcium-silicate-based sealer versus resin-based sealer when combined with gutta-percha using the single-file system XP-endo Rise.Materials and methods: 26 canals in typodont acrylic Nissin teeth were instrumented with E-flex blue rotary instruments up tosize 0.35mm and taper of 4%. Root canals were divided into two groups, obturated using either hydraulic condensation techniquewith a bioceramic sealer (n=13) or continuous wave of compaction (CWC) technique with a resin-based sealer (n=13). Theretreatment procedure was done for both groups using the XP-endo Rise instruments. The time required to complete theretreatment procedures …

Post-Operative Pain After Non-Surgical Root Canal Treatment With A Single Cone/ Bio-Ceramic Sealer Based Obturation: A Randomized Clinical Trial, Shehabeldin Mohamed Saber Prof.Dr.

Dentistry

Aim: compare the post- operative pain associated with sealer-based obturation technique (SBO) using bioceramic sealer and a (CWC) obturation technique with a resin-based sealer.

Materials and methods: two-arm randomized clinical trial with 2 parallel groups 20 patients each, after shaping the canals to a minimum dimension of 30/.04, except for a single middle mesial canal, which was shaped to 25/.04 patients randomly allocated into two groups based on the obturation material used. To ensure patient privacy, each name was replaced with a case number. Patients documented their pain on a 0–10 (VAS) at: 4 hours, one day, and two days …

Intracanal Microbial Reduction After Root Canal Preparation With Different Tapered Instruments. An Ex-Vivo Study, Shehabeldin Mohamed Saber Prof.Dr.

Dentistry

Aim: The objective of this ex vivo study was to assess the dual impact of instruments taper and irrigation activation methods on bacterial reduction in infected root canals.

Materials and methods:42 extracted human molar teeth were inoculated with Enterococcus faecalis for 2 weeks to develop a mature biofilm. The samples were divided into four experimental groups and two control groups. The experimental groups underwent root canal preparation using two different instruments: XP Shaper (taper 0.04) and Reciproc Blue R25 (taper 0.08). Additionally, two different activation methods were tested: mechanical (XP Finisher) and sonic (EDDY). Bacterial reduction was assessed using Colony …

Accuracy Of Endodontic Access Guides Printed By A Costefficient 3d Printer (An In-Vitro Study), Shehabeldin Mohamed Saber Prof.Dr.

Dentistry

Aim: This study evaluated the precision and trueness of endodontic access guides fabricated using a cost-effective desktop LCD 3D

printer compared to a higher-cost SLA printer for guided endodontics.

Materials and methods: Thirty-two root canals from Nissin typodont teeth were divided into two groups: LCD and SLA. Virtual

planning was performed, and endodontic guides were fabricated using both 3D printing technologies. Pre-operative and postoperative

cone beam tomography (CBCT) scans were taken, and the linear deviation between the planned and actual access paths

was measured at two axial levels: the occlusal entry point and the canal orifice. Statistical analyses were conducted …

Retreatability Of Bioceramic And Resin-Based Root Canal Sealers Using Xp Shaper Rise: An In Vitro Study, Shehabeldin Mohamed Saber Prof.Dr.

Dentistry

Aim: The purpose of this study was to investigate in-vitro the retreatability of premixed calcium-silicate-based sealer versus resinbased

sealer when combined with gutta-percha using the single-file system XP-endo Rise.

Materials and methods: 26 canals in typodont acrylic Nissin teeth were instrumented with E-flex blue rotary instruments up to

size 0.35mm and taper of 4%. Root canals were divided into two groups, obturated using either hydraulic condensation technique

with a bioceramic sealer (n=13) or continuous wave of compaction (CWC) technique with a resin-based sealer (n=13). The

retreatment procedure was done for both groups using the XP-endo Rise instruments. The time required …

Intracanal Microbial Reduction After Root Canal Preparation With Different Tapered Instruments. An Ex-Vivo Study, Hala Fayek Khalil, Shehab Eldin Moahmed Saber, Mostafa Kholy, Soha Elhady, Esraa Abdelwahab

Dentistry

Aim: The objective of this ex vivo study was to assess the dual impact of instruments taper and irrigation activation methods on bacterial reduction in infected root canals. Materials and methods:42 extracted human molar teeth were inoculated with Enterococcus faecalis for 2 weeks to develop a mature biofilm. The samples were divided into four experimental groups and two control groups. The experimental groups underwent root canal preparation using two different instruments: XP Shaper (taper 0.04) and Reciproc Blue R25 (taper 0.08). Additionally, two different activation methods were tested: mechanical (XP Finisher) and sonic (EDDY). Bacterial reduction was assessed using Colony …

Cytocompatibility, Osteogenic Potential, Antibacterial And Antibiofilm Efficacy Of A Calcium‑Silicate‑Based Intracanal Medication, Shehabeldin Mohamed Saber Prof.Dr., Shereen Nader

Dentistry

This study assessed the biological and antimicrobial properties of a calcium silicate-based material and calcium hydroxide (CH) when used as intracanal medications. MTT assay, osteogenic differentiation of human periodontal stem cells (hPDLSCs), cell mineralization-assay, and determination of ALP activity were assessed to investigate the biological properties. While the agar well diffusion, crystal violet (CRV) assay and LIVE/DEAD staining of dentin slices infected with a mature E. faecalis biofilm were used to assess the antimicrobial properties. Normally distributed data were analyzed using one and two-way ANOVA and post hoc Tukey test, while for non-normally distributed data Kruskal Wallis and Dunn's tests …

Ex-Vivo Evaluation Of Clinically-Set Hydraulic Sealers Used With Di!Erent Canal Dryness Protocols And Obturation Techniques: A Randomized Clinical Trial, Shehabeldin Mohamed Saber Prof.Dr.

Dentistry

Objectives This 2-part randomized parallel triple-blind clinical trial adopts a unique model assessing clinically-set hydraulic

calcium silicate-based sealers (HCSBS) after different root canal dryness protocols and obturation techniques.

Methods For the first phase of the study, 24 teeth scheduled for orthodontic extractions were allocated into four groups

according to the canal dryness protocol and the obturation technique. G1 (CLC-AHP): cold lateral compaction (CLC) with

AH Plus sealer, G2 (CLC-ES-SD): CLC with Endosequence (ES) after standard canal(s) dryness (SD); G3 (SC-ES-SD):

matching single-cone (SC) with ES after SD; G4 (SC-ES-PD): as G3 but after partial canal(s) dryness (PD). Teeth were

extracted …

Antimicrobial Efficacy Of Different Intracanal Medicaments Used In Regenerative Endodontics Against A Mature Multi-Species Biofilm Model (An Ex-Vivo Study), Shehabeldin Mohamed Saber Prof.Dr.

Dentistry

Introduction: The aim of this study was to compare the efficacy of different antimicrobial agents, including Ciprofloxacin, Doxycycline, Clindamycin, Metronidazole, Amoxicillin and Clavulanate, Triple antibiotic paste (TAP) and Calcium hydroxide (CH), as intra-canal medications for regenerative endodontics against an aged, multispecies ex-vivo biofilm model.

Methods: A total of one hundred fifty single-rooted human teeth were used in this study. Fifteen teeth were used to monitor the formation and maturation of a multispecies biofilm model consisting of Enterococcus faecalis, Staphylococcus epidermidis and Candida albicans. After 21 days of incubation, the other one-hundred and thirty-five teeth were divided into 9 equal …

Does Reduced Taper Canal Preparation Increase The Load Capacity Of Endodontically Treated Teeth?, Shehabeldin Mohamed Saber Prof.Dr.

Dentistry

Objectives: The aim of this in-vitro study is to assess and compare the load capacity of teeth with canals prepared using progressive taper versus regressive taper.

Methods: Twenty-seven extracted mandibular molars were categorized into three equal groups. The negative control group involved teeth that were accessed and restored with composite resin without canal preparation. The regressive canal preparation group used the TruNatomy file system, while the progressive canal preparation group employed the ProTaper Gold system. Subsequently, all samples underwent loading using a universal testing machine until tooth fracture occurred. The force required to fracture each tooth was recorded in Newton. …

Evaluation Of The Quality Of Root Canal Obturation Using Resin And Silicon-Based Sealers: A Randomized Clinical Trial, Shehabeldin Mohamed Saber Prof.Dr.

Dentistry

Aim: to assess and compare the quality of root canal obturation using AH Plus and GuttaFlow 2 in patients with symptomatic

irreversible pulpitis.

Materials and methods: This randomized clinical trial included 50 participants with mandibular molars diagnosed with

symptomatic irreversible pulpitis. Participants were randomly assigned to two groups: the AH Plus group (RBS) and the

GuttaFlow 2 group (SBS). Clinical procedures included local anesthesia, access cavity preparation under a surgical microscope,

rubber dam isolation, and root canal preparation using the ProTaper Gold system. For the RBS group, AH Plus sealer was used

with cold lateral condensation, while for the SBS …

Evaluation Of The Quality Of Root Canal Obturation Using Resin And Silicon-Based Sealers: A Randomized Clinical Trial, Kareem Darwish, Engy M. Kataia, Shehabeldin Saber

Dentistry

Aim: to assess and compare the quality of root canal obturation using AH Plus and GuttaFlow 2 in patients with symptomatic irreversible pulpitis. Materials and methods: This randomized clinical trial included 50 participants with mandibular molars diagnosed with symptomatic irreversible pulpitis. Participants were randomly assigned to two groups: the AH Plus group (RBS) and the GuttaFlow 2 group (SBS). Clinical procedures included local anesthesia, access cavity preparation under a surgical microscope, rubber dam isolation, and root canal preparation using the ProTaper Gold system. For the RBS group, AH Plus sealer was used with cold lateral condensation, while for the SBS …

Quantifying Radiographs In Endodontics: A Survey, Pavneet K. Bains, Albert F. Mcmullen Iii, Haley D. Kessler, Andrew G. Chapple

School of Dentistry Faculty Publications

Purpose: The aim of this study was to investigate the average quantity of radiographs obtained during non-surgical endodontic treatment and trends in endodontic radiography among U.S. members of the AAE. Materials & Methods: A survey was sent via e-mail to 4,269 active U.S. members of the AAE. Data were collected over a period of approximately 4 months. The number of radiographs reported for non-surgical endodontic treatment was compared across fourteen variables using Kruskal Wallace tests. Chi Square tests of Independence were also used to evaluate trends in endodontic radiography based on three primary categorical variables of interest: years of experience, …

Endodontic Microsurgery Using Dynamic Navigation And Trephine Burs, Trenton Smith, Sameer Jain, Jeffery Nick Sanders, Michael Block, Mark Odom, Albert F. Mcmullen

School of Dentistry Faculty Publications

Objectives: The use of the surgical operating microscope, CBCT imagining, biocompatible filling materials and ultrasonic instruments have improved the success and predictability of apical surgery. Despite these advances, more accurate and precise techniques for creating osteotomies and root-resections (apicoectomies) have not been widely adopted. This study highlights how dynamic navigation systems (DNS) can help avoid unnecessary dentin removal and “mitigate iatrogenic errors” such as root perforation. Methods: A total of 56 root-end resections were planned and completed on two sets of identical TrueJaw Endosurg models (DE Labs, Santa Barbra, CA, USA). One set of maxillary and mandibular jaws were used …

Stratasys Printed 3-D Mandibular Molar With Tooth Anatomy And Texture, M. Austin White, Charles E. Taylor, Edwin Kee, Albert F. Mcmullen

School of Dentistry Faculty Publications

Introduction: We used CBCT scans of a human mandibular molar in concert with the Stratasys 3-D printer to fabricate said teeth with similar texture and hardness to enamel, dentin, and cementum. The printed tooth should also have the same crown and root anatomy, as well as root canal anatomy of the scanned tooth. Methods: One mandibular first molar was scanned (Carestream Dental CS 9600 CBCT) and printed (Stratasys 3D multi-material printer). A (Micromet 5104) hardness tester was used to test the hardness of the printed tooth. Nine combinations of two different materials (Stratasys’ Vero Pure White and Agilus 30 Clear …

Effect Of Different Calcium Hydroxide Formulations Used During Regenerative Endodontic Procedures On The Viability, Mineralization, And Dentino/Cemento/Osteogenic Differentiation Potential Of Human Periodontal Ligament Stem Cells. An In-Vitro Study., Mohamed Medhat Kataia, Muhammed Abouel Seoud, Shehab Eldin Saber, Shereen Nader, Hadeel Mousa

Dentistry

Aim: To evaluate the effect of CH and NCH on the viability, attachment, osteogenic, cementogenic and dentinogenic differentiation potential of hPDLSCs in-vitro.
Materials and Methods: Calcium Hydroxide (CH) and nano calcium hydroxide (NCH) powders were initially characterized using transmission electron microscope (TEM), X-ray Diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, and a zeta sizer instrument. Standard dentin discs were prepared, irrigated according to the Regenerative Endodontic Procedures (REP) protocol, then treated with 1 mg/ml of CH or NCH pastes, and incubated for 1 week. Untreated discs served as control. Then, they were washed off, and all discs were treated with …

Would Access Cavity Design Affect The Mechanical Behavior Of Tooth: A Finite Element Study, Mohamed Ashraf Elhusseini1, Hala Fayek Khalil, Engy M. Kataia

Dentistry

Introduction: Two distinct access cavity designs were used to access Two simulated finite element

models (FE) Lingual (LAC) and incisal (IAC)). Three experimental FE models, including the IT

model, were developed: LAC and IAC.

Methods: For every simulated model, one distinct radicular preparations was carried out of size

#30/.04. The incisal edge was subjected to an occlusal cycle load of 120 N. The maximum von

mises stresses (VMS), maximum principal stresses (MPS), and stress distribution patterns were

assessed and calculated quantitatively.

Results: The traditional access design showed slightly higher fracture resistance to loading than

the conservative incisal access cavity design. …

The Use Of Tissue Grafts Associated With Immediate Implant Placement To Achieve Better Peri-Implant Stability And Efficacy: A Systematic Review And Meta-Analysis, Enrico Maria Rondone, Bruno Leitão-Almeida, Miguel Silva Pereira, Gustavo Fernandes, Tiago Borges

MOSDOH Faculty Publications

Background: The goal of this systematic study and meta-analysis was to evaluate the efficacy of hard and/or soft tissue grafts associated with type-1 implants on healing and treatment outcomes. The primary outcomes studied were implant survival rate, pocket depth, marginal periimplant recession, bone loss, bone thickness (volumetric change), interproximal bone level, mesial and distal papilla migration, and radiographic evaluation; and the secondary parameters were Pink Esthetic Score (PES), vertical distance from implant shoulder and bone, Visual Analogue Score (VAS), Implant Stability Quotient (ISQ), and biological complications (fistulas, pain, mucositis, and peri-implantitis). Methods: The PICO strategy was used to formulate the …

Fibroblasts And Osteoblasts Behavior After Contact With Different Titanium Surfaces Used As Implant Abutment: An In Vitro Experimental Study, Jose Henrique Cavalcanti De Lima, Patricia Cristina Matos Robbs, Elena Mavropoulos Oliveira Tude, Piedad N. De Aza, Eleani Maria Da Costa, Antonio Scarano, Juan Carlos Prados-Frutos, Gustavo Fernandes, Sergio Alexandre Gehrke

MOSDOH Faculty Publications

Background: The goal of this in vitro study was to compare three different surfaces: two types of implant surfaces commercially available ([a] smooth/machined and [b] acid-treated surface) versus (c) anodized surface. Discs were manufactured with commercially pure titanium (CP) grade IV, which were subsequently analyzed by scanning microscopy and fibroblastic and osteoblastic cell cultures. Methods: Ninety-nine discs (5 × 2 mm) were manufactured in titanium grade IV and received different surface treatments: (i) Mach group: machined; (ii) AA group: double acid etch; and (iii) AN group: anodizing treatment. Three discs from each group were analyzed by Scanning Electron Microscopy (SEM) …