Dentistry Commons^™

Antibiotic-Loaded Dendrimer Hydrogels In Periodontal Bone Regeneration: A Feasibility Study, Nicholas G. Yesbeck

Theses and Dissertations

Purpose: Prescription of a complete course of oral antibiotics following bone grafting procedures is a common clinical practice in surgical periodontics. Prophylactic administration of antibiotics reduces the risk of surgical site infection and systemic administration is preferred over local delivery because of short duration of action in the latter circumstance. The goal of this study is to demonstrate the ability of dendrimer hydrogels (DH) to prolong the release kinetics of antibiotics in-vitro. A secondary goal will be to analyze the effect of different particulate bone allografts on the release kinetics of the antibiotic.

Methods: Dendrimer hydrogels (DH) were synthesized …

Ck2 Phosphorylation Of Human Papillomavirus 16 E2 On Serine 23 Promotes Interaction With Topbp1 And Is Critical For E2 Plasmid Retention Function, Apurva Tadimari Prabhakar

Theses and Dissertations

Human papillomaviruses (HPVs) are the causative agents in most cervical cancers and have been implicated in a rising number of oropharyngeal cancers. HPV positive oropharyngeal cancers have increased significantly over the last two decades with no specific anti-viral therapeutic options for the treatment of HPV diseases. During its lifecycle, HPV16 encoded E2 protein interacts with host cellular factors to regulate viral transcription, replication and genome segregation/retention. Our understanding of the cellular partner proteins that E2 uses to mediate its functions remains incomplete.

Previously, an interaction between E2 and the host protein TopBP1 was identified. In earlier studies, it was also …

Il-36g Cytokine Induction In Oral Epithelial Cells Triggered By Treponema Denticola And Their Role In Chronic Periodontitis, Christopher Blake

Theses and Dissertations

Periodontal disease is a polymicrobial disease affecting 10-15% of the population. Periodontal disease affects the quality of life by reducing mastication and potentially causing tooth loss. Periodontal disease is also linked to more severe, systemic diseases such as cardiovascular disease and rheumatoid arthritis. Development of the diseased state occurs when the plaque biofilm shifts from healthy to dysbiotic. The shift to a dysbiotic biome brings with it a shift in bacterial presence, specifically an increase in gram-negative bacteria, such as T. denticola. T. denticola is a gram-negative, anaerobic spirochete whose relationship with oral epithelial cells is poorly understood. Preliminary data …

Validation Of A Screening Tool For Pediatric Obstructive Sleep Apnea: A Pilot Study, Julia A. Giardina

Theses and Dissertations

Purpose: The purpose of this study was to evaluate the Pediatric-Modified-STOP-Bang (PM-STOP-Bang) as a screening tool to assess sleep related breathing disorders (SRBD) within a pediatric population. The specific aims of this study were: 1) compare the PM-STOP-Bang scores with those from the validated Pediatric Sleep Questionnaire (PSQ), 2) determine the ability of the PM-STOP-Bang and the PSQ screening tools to accurately detect children of high risk for SRBD from a one-night home sleep apnea test (HSAT).

Methods: Orthodontic patients were screened using the PM-STOP-Bang at VCU Graduate Clinic. Patients (n=10) who were recruited and enrolled. Five were determined to …

Radiographic Comparison Using Cbct-Pai And Volumetric Analysis Of Endodontically Treated Teeth: A Retrospective Cohort Study, Jeremy Hargrove

Theses and Dissertations

Purpose: To assess the changes in CBCT-PAI score as well as 3-D volume of periapical findings associated with endodontically treated roots and to see how these findings change over time.

Methods: Retrospective chart review with the following inclusion criteria: 1. At least one tooth was endodontically treated at the endodontic private practice; 2. Patient had a recall examination and the treated tooth was asymptomatic, functional and a CBCT was exposed; 3. CBCT interpretation found a periapical finding associated with the treated tooth; 4. A follow-up recall examination was completed and CBCT exposed. Cases that fulfilled the inclusion criteria had the …

Reproducibility And Accuracy Of The Virtual Occlusal Record, Anne M. Harper

Theses and Dissertations

Purpose: To evaluate the reproducibility and accuracy of the virtual occlusal record compared to the gold standard physical bite record

Methods: Two physical bite records of PVS and two virtual bites using an iTero Element scanner were obtained from 22 participants. Intraoral scans were exported as STL files, and virtual occlusal records were created. CBCT images were taken of physical occlusal records and converted to STL files. STL files were superimposed using Geomagic Control X™ Software. Root mean square (RMS) and percent similarity within 0.25 mm tolerance were compared at a significance level of 0.05.

Results: Average …

Virtual Reality Tool For Visualizing The Oral Microbiome, Alexus M. Harris

Theses and Dissertations

There are limited teaching tools for helping both students and patients understand the oral microbiome. It is important to understand the oral microbiome as it heavily influences the health of your oral cavity and the dysbiosis of disease. The oral microbiome influences other aspects of health and serves as an indicator for health. With a greater understanding of the oral microbiome, patients can make healthier lifestyle changes and dentists can better educate their patients. For that reason, the goal of this project was to create a virtual reality tool for visualizing the oral microbiome. The assets for the VR tool …

In Vitro Evaluation Of The Solubility Of Endosequence Bc Sealer In Acidic Solutions, Inderpal S. Sappal

Theses and Dissertations

Purpose: In recent years, bioceramic sealers (BCS), such as EndoSequence BC Sealer, have gained popularity in endodontic practices. Previous research has found BCS difficult to remove from canals during retreatments when compared with other endodontic sealers. Acidic solutions have been found to inhibit the set of BCS and increase their solubility. The purpose of this study was to evaluate the efficacy of acidic solutions in the removal of BCS as compared with a traditional retreatment solvent.

Methods: Roots of extracted human single rooted teeth (N=15) were prepared into 17 mm sections, then instrumented with ProTaper Gold (PTG) rotary files up …

The Effect Of Local Anesthesia When Used In Dental Restorative Cases Under General Anesthesia On Control Of Intraoperative Physiologic Parameters And Post-Operative Comfort, Kathryn S. Skarda

Theses and Dissertations

Purpose:

To determine the effect of local anesthesia on post-operative pain and physiologic parameters intraoperatively in patients undergoing dental care under general anesthesia.

Methods:

This study was modeled as a double-blinded randomized control trial. Patients included healthy children under the age of six scheduled for restorative dental treatment under general anesthesia. Patient behavior was evaluated pre and post-operatively using the FLACC score and post-operatively using the Parental Pain Score Measure. Intraoperatively, all participants followed a standardized general anesthesia protocol and were given either local anesthetic infiltration by the dental surgeon performing the surgery or no anesthesia was utilized. Intraoperatively physiologic …

Recent Developments In Targeting Genes And Pathways By Rnai-Based Approaches In Colorectal Cancer, Asiyeh Jebelli, Behzad Baradaran, Jafar Mosafer, Amir Baghbanzadeh, Ahad Mokhtarzadeh, Lobat Tayebi

School of Dentistry Faculty Research and Publications

A wide spectrum of genetic and epigenetic variations together with environmental factors has made colorectal cancer (CRC), which involves the colon and rectum, a challenging and heterogeneous cancer. CRC cannot be effectively overcome by common conventional therapies including surgery, chemotherapy, targeted therapy, and hormone replacement which highlights the need for a rational design of novel anticancer therapy. Accumulating evidence indicates that RNA interference (RNAi) could be an important avenue to generate great therapeutic efficacy for CRC by targeting genes that are responsible for the viability, cell cycle, proliferation, apoptosis, differentiation, metastasis, and invasion of CRC cells. In this review, we …

Simultaneous Selective Enrichment Of Methylparaben, Propylparaben, And Butylparaben From Cosmetics Samples Based On Syringe-To-Syringe Magnetic Fluid Phase Microextraction, Ebrahim Alipanahpour Dil, Mehrorang Ghaedi, Arash Asfaram, Lobat Tayebi

School of Dentistry Faculty Research and Publications

Present work is the preparation of novel magnetic nanofluids based on deep eutectic solvent and used for the rapid microextraction of methylparaben (MP), propylparaben (PP), and butylparaben (BP) from cosmetics samples using syringe-to-syringe dispersive magnetic nanofluid microextraction procedure (SS-DMNF-ME). The optimization of the extraction of MP, PP, and BP was performed through central composite design (CCD). The optimum extraction conditions were assessed by optimizing pH, nanofluid volume, NaCl concentration, cycle number, and methanol volume. pH 8.0, 200 μL of magnetic nanofluid, 6% w/v of NaCl, eight cycles of injection/back injection, and 80 μL of methanol were the optimum extraction conditions, …

The Use Of Mineral Trioxide Aggregate In The Treatment Of Horizontal Root Fractures: A Case Presentation And Literature Update, Elif Ballikaya, H. Cem Güngör

School of Dentistry Faculty Research and Publications

Root fracture is a rarely seen traumatic injury in permanent dentition. It has a prevalence of 0.5-7 %. Tooth survival is significantly affected by the type of healing and the location of the root fracture. Additionally, the patient’s age, stage of root development, mobility and dislocation of the coronal fragment, and diastasis between fragments following this kind of injury are among the important factors. In this case presentation, the management with MTA plug and 6-year follow-up of a horizontal root fracture was reported. A literature update regarding the use of MTA in the management of horizontal root fractures was also …

Inventing Dental Continuing Education: History Of The Dental Forum -- Milwaukee, Kenneth J. Waliszewski

School of Dentistry Faculty Research and Publications

Life-long learning is an ancient concept. The young are unaware of it. A beginning practitioner, often blinded by ego, ignores its value. The seasoned veteran, haunted by challenges, wonders how it passed him by. History can and does often teach us lessons. In 1787, Benjamin Franklin stood up to address the American Constitutional Convention and urged his fellow patriots to sign the document. At the age of 81, he was the oldest person in the group. He acknowledged having opened his mind to contrarian viewpoints from his younger colleagues. Franklin spoke these words: “If you are convinced that you know …

Bioresorbable Composite Polymeric Materials For Tissue Engineering Applications, Sakineh Hajebi, Saeed Mohammadi Nasr, Navid Rabiee, Mojtaba Bagherzadeh, Sepideh Ahmadi, Mohammad Rabiee, Mohammadreza Tahriri, Lobat Tayebi, Michael R. Hamblin

School of Dentistry Faculty Research and Publications

This review covers the development of bioresorbable polymeric composites for applications in tissue engineering. Various commercially available bioresorbable polymers are described, with emphasis on recent bioresorbable composites based on natural and synthetic polymers. Bioresorbable polymers contain hydrolyzable bonds, which are subjected to chemical degradation via either reactive hydrolysis or enzyme-catalyzed active hydrolysis. For synthetic polymers, chemical hydrolysis is the most important mode of degradation. The degradation rate can be controlled by varying the molecular weight and crystallinity. Examples of bioresorbable polymers are: polyurethane, poly(D,L)lactide, poly(lactic-co-glycolic) acid, poly(α-hydroxy acids), cross-linked polyester hydrogels, poly(orthoesters), polyanhydrides and polyethylene glycol.

Spect/Ct Correlation In The Diagnosis Of Unilateral Condilar Hyperplasia, Diego Fernando López, Valentina Borrás Ríos Borrás, Juan Manuel Muñoz, Rodrigo Cardenas-Perilla, Luis Eduardo Almeida

School of Dentistry Faculty Research and Publications

Objective:

To evaluate the correlation between metabolic bone activity measured by single photon emission computed tomography (SPECT) and the anatomic condylar characteristics acquired by computed tomography (CT), in patients with unilateral condylar hyperplasia (UCH).

Method and Materials/Patients:

Observational, descriptive study in a group of 71 patients with clinical diagnosis of UCH and indication of SPECT/CT. Bone SPECT images obtained in a gamma-camera GE Infina and processed in a station Xeleris 3 with the program Volumetrix MI Evolution for bone. CT images acquired in a PET/CT Biograph mcT20 equipment (Siemens) processed in a station Osirix V 7.5.1 (Pixmeo, Bomex, Switzerland).

Results: …

A 3d Nanostructured Calcium-Aluminum-Silicate Scaffold With Hierarchical Meso-Macroporosity For Bone Tissue Regeneration: Fabrication, Sintering Behavior, Surface Modification And In Vitro Studies, Ashkan Bigham, Amir Hamed Aghajanian, Mehdi Movahedi, Mansoureh Sattary, Mohammad Rafienia, Lobat Tayebi

School of Dentistry Faculty Research and Publications

This is a comprehensive study reporting the fabrication of highly porous Gehlenite scaffold (Ca₂Al₂SiO₇)—both with and without surface modification—for the first time. The sintering temperature of Gehlenite scaffolds was optimized. Next, the optimized Gehlenite scaffold was coated by polycaprolactone (PCL)-Forsterite (Mg₂SiO₄) nanocomposite to improve the scaffold’s brittleness and biological properties. 1375 °C was found to be the optimized sintering temperature by which the Gehlenite scaffold was consolidated. Different PCL and Forsterite concentrations were separately applied on the optimized scaffold to yield a complete nanocomposite coating without clogging the macroporous structure. …

Macro-And Micromechanical Modelling Of Ha-Elastin Scaffold Fabricated Using Freeze Drying Technique, Matin Mohammadzadeh Rad, Saeed Saber-Samandari, Mojtaba Sadighi, Lobat Tayebi, Mohammad Mohammadi Aghdam, Amirsalar Khandan

School of Dentistry Faculty Research and Publications

No abstract provided.

Inhibition Of Candida Albicans And Mixed Salivary Bacterial Biofilms On Antimicrobial Loaded Phosphated Poly(Methyl Methacrylate), Andrew R. Dentino, Dong Hwa Lee, Kelley Dentino, Arndt Guentsch, Mohammadreza Tahriri

School of Dentistry Faculty Research and Publications

Biofilms play a crucial role in the development of Candida-associated denture stomatitis. Inhibition of microbial adhesion to poly(methyl methacrylate) (PMMA) and phosphate containing PMMA has been examined in this work. C. albicans and mixed salivary microbial biofilms were compared on naked and salivary pre-conditioned PMMA surfaces in the presence or absence of antimicrobials (Cetylpyridinium chloride [CPC], KSL-W, Histatin 5 [His 5]). Polymers with varying amounts of phosphate (0–25%) were tested using four C. albicans oral isolates as well as mixed salivary bacteria and 24 h biofilms were assessed for metabolic activity and confirmed using Live/Dead staining and confocal microscopy. …

Zirconia Restorations: An American Dental Association Clinical Evaluators Panel Survey, Nathaniel C. Lawson, Kevin Frazier, Ana K. Bedran-Russo, Sharukh Khajotia, Jacob Park, Olivia Urquhart

School of Dentistry Faculty Research and Publications

Background

Zirconia is a relatively new dental material used for indirect dental restorations. Little is known about how dental practitioners are using this material in their practice.

Methods

A survey on zirconia restorations was developed and administered electronically through e-mail communications to the American Dental Association Clinical Evaluators (ACE) Panel on August 31, 2020. Reminders were sent to nonrespondents, and the survey closed 2 weeks after the launch date.

Results

When using zirconia for a restoration, respondents choose it to restore natural teeth (99%) more often than implants (76%). Almost all respondents (98%) use it for posterior crowns, whereas approximately …

Concurrent Application Of Conductive Biopolymeric Chitosan/ Polyvinyl Alcohol/ Mwcnts Nanofibers, Intracellular Signaling Manipulating Molecules And Electrical Stimulation For More Effective Cardiac Tissue Engineering, Ali Abedi, Behnaz Bakhshandeh, Ali Babaie, Javad Mohammadnejad, Sadaf Vahdat, Reza Mombeiny, Seyed Reza Moosavi, Javid Amini, Lobat Tayebi

School of Dentistry Faculty Research and Publications

Fabrication of appropriate electro-conductive scaffold, application of small molecules (SMs), electrical stimulation (ES), and stem cells are steps forward in cardiac tissue engineering. Herein, for the first time, all mentioned factors have been taken into account concurrently regarding the differentiation of unrestricted somatic stem cells (USSCs) into cardiac cells. To accomplish this goal, electrospun composite scaffolds made of chitosan (CS) and polyvinyl alcohol (PVA) with multi-wall carbon nanotubes (MWCNTs; ranged from 0 to 2.5% w/w) were fabricated. After analyzing mechanical, electrical, and biological properties, the best MWCNTs portion was selected. Of note, the addition of 2%w/w MWCNTs to the CS/PVA …

Current Trends In The Management Of Endodontic Emergencies, Asmi Shah

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction: The management of endodontic emergencies has changed over the last few decades and varies from practice to practice. This is due to the development of new materials, new irrigation techniques, and new evidence-based research which supports clinical success. An updated questionnaire is necessary to further advance the clinical knowledge in endodontic emergencies based on current practices. Therefore, the purpose of this research is to determine the difference practice modalities between members who are board certified, who are not board certified, and endodontic graduate residents.

Materials and Methods: In 2021, every member of the American Association of Endodontics …