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Full-Text Articles in Nucleic Acids, Nucleotides, and Nucleosides
Fluorescently Labeled Sirnas And Their Theranostic Applications In Cancer Gene Therapy, Stephen David Kozuch
Fluorescently Labeled Sirnas And Their Theranostic Applications In Cancer Gene Therapy, Stephen David Kozuch
Seton Hall University Dissertations and Theses (ETDs)
Gene therapy has emerged as a promising precision nano-medicine strategy in the treatment of numerous diseases including cancer. At the forefront of its utility are the applications of short-interfering RNA (siRNA), that silence oncogenic mRNA expression leading to cancer cell death through the RNA interference (RNAi) pathway. Despite the therapeutic potential, siRNAs are limited by poor pharmacological properties, which has hindered their translation into the clinic. Recent studies, however, have highlighted the applications of modified siRNAs, including the use of fluorescent probes and siRNA nanostructures in cancer detection and treatment. The siRNAs reported in this thesis are designed to target …
Optimization Of The Split-Spinach Aptamer For Monitoring Contiguous Rna Nanoparticle Assembly, Jack M. O'Hara
Optimization Of The Split-Spinach Aptamer For Monitoring Contiguous Rna Nanoparticle Assembly, Jack M. O'Hara
Honors Projects
The emerging field of RNA nanotechnology takes advantage of the RNA’s ability to self-assemble into exquisite structures. As nanoparticle design continues to advance and move into increasingly complex biological systems, tools to monitor their assembly and location will be of great importance. Here, a split-aptamer system is used to monitor assembly of a six-membered nanoring based on fluorescence feedback of a fluorophore. First, the split-aptamer is designed into two of the six pieces of the ring. Through mutation and deletion, we optimize the fluorescence feedback established when a six membered nanoparticle assembles, compared to partial assembly. We demonstrate that with …
Design And Syntheses Of Fluorescent Cytosine Analogues, David W. Dodd
Design And Syntheses Of Fluorescent Cytosine Analogues, David W. Dodd
Electronic Thesis and Dissertation Repository
The avid hybridization of peptide nucleic acid (PNA) to DNA and RNA along with the molecule’s biological stability has led it to be used in both antisense and antigene capacities. PNA acts against translation via a steric blockade mechanism. It is therefore reasonable to assume that increased heteroduplex stability could lead to increased potency. Two ways of doing this were explored. I) N-Terminal attachment of a platinous chloride chelating moiety to PNA complementary to Xenopus noggin was synthesized with the objective of selective, covalent platination of the target transcript in vivo. Phenotypes consistent with knockdown of the selected …