Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Biological Factors
Methionine Restriction Enhances Platinum-Based Chemotherapy-Induced Immunogenic Cell Death In Triple Negative Breast Cancer Cells, Mallory Thompson, Laura Sipe Ph.D.
Methionine Restriction Enhances Platinum-Based Chemotherapy-Induced Immunogenic Cell Death In Triple Negative Breast Cancer Cells, Mallory Thompson, Laura Sipe Ph.D.
Departmental Honors & Graduate Capstone Projects
When cancer cells are treated with chemotherapy, they can release Damage-Associated Molecular Patterns (DAMPs) as they die that can alert and engage the immune system, a process known as an immunogenic cell death (ICD). The dying cancer cell can release certain molecules, including ATP or calreticulin, that immune cells first recognize as abnormal before initiating an anti-tumor response. However, not all chemotherapies cause the release of these beneficial signals as they kill the cell. This study aims to determine whether a specific deprivation technique, called methionine restriction, enhances the ICD process and DAMP expression when used in conjunction with chemotherapy …
5-Fluorouracil Induced Oxidative Stress Is Variable Across Triple Negative Breast Cancer Cell Lines, Rachel L. Walker
5-Fluorouracil Induced Oxidative Stress Is Variable Across Triple Negative Breast Cancer Cell Lines, Rachel L. Walker
Departmental Honors & Graduate Capstone Projects
Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer with treatment limited to chemotherapy. However, not all patient tumors respond to chemotherapy. The variable cytotoxic effects of chemotherapies may be due to how cancer cells regulate reactive oxygen species (ROS). In this study, we examined two mouse breast cancer cell lines: 4T1 and EO771, vary when treated with hydrogen peroxide (H2O2) and 5-Fluorouracil (5FU), known ROS-inducing substances. In 4T1 cells H2O2 increased oxidized DNA but caused minimal cell death. While in EO771 cells that same amount of H2O2 caused complete apoptosis. Cell number, ROS, and cell death showed …